Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,928,799 A→C 9.6% L14R (CTT→CGT)  ptrB ← protease II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,928,7990AC 9.6% 331.1 / 24.7 128L14R (CTT→CGT) ptrBprotease II
Reads supporting (aligned to +/- strand):  ref base A (55/60);  new base C (6/6);  total (62/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-02

Only 100 of 133 total aligned reads displayed.

CCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGAT  >  NC_000913/1928650‑1928948
                                                                                                                                                     |                                                                                                                                                     
ccTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGaa                                                                                                                                                       <  1:627067/150‑1 (MQ=255)
ccTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCAAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGACAGTAGTAATTATCGATGCGCGTATCGCCATGaa                                                                                                                                                       >  1:680963/1‑150 (MQ=255)
 cTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACATCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGaac                                                                                                                                                      >  2:210178/1‑149 (MQ=255)
   ttAAGATGCGATCCTGCAAGGCTTGGTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCg                                                                                                                                                    <  2:58373/150‑1 (MQ=255)
     aaGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTc                                                                                                                                                  >  2:382364/1‑150 (MQ=255)
       gATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCAt                                                                                                                                                >  1:1535629/1‑150 (MQ=255)
        aTGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATg                                                                                                                                               >  2:771974/1‑150 (MQ=255)
           cGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCCGCCAGTAGTAATTATCGATGCGCGTAAAGCCATGAAGCGTCATgcag                                                                                                                                            >  2:335545/1‑147 (MQ=255)
           cGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGACGCTTCATGGCg                                                                                                                                            <  2:29887/150‑1 (MQ=255)
           cGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGACGCTTCATGGCg                                                                                                                                            <  2:53628/150‑1 (MQ=255)
           cGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAATCGTCATGGCg                                                                                                                                            >  1:1036023/1‑150 (MQ=255)
           cGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGATGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGACGCTTCATGGCg                                                                                                                                            <  1:1067582/150‑1 (MQ=255)
            gATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGt                                                                                                                                           >  2:1595434/1‑150 (MQ=255)
             aTCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCAGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGACGCTTCATGGCgat                                                                                                                                          <  2:1679074/150‑3 (MQ=255)
               ccTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGACGCTTCATGGCgatac                                                                                                                                        <  1:1273375/150‑5 (MQ=255)
               ccTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTggg                                                                                                                                        <  1:1572439/150‑1 (MQ=255)
                cTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTgggg                                                                                                                                       >  1:361822/1‑150 (MQ=255)
                cTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGGCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTgggg                                                                                                                                       <  2:309317/150‑1 (MQ=255)
                    aaGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATg                                                                                                                                   <  2:919722/150‑1 (MQ=255)
                           gttgtGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgcgggcgg                                                                                                                            <  2:1725997/150‑1 (MQ=255)
                             tgtgAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCt                                                                                                                          >  1:960951/1‑150 (MQ=255)
                                 cggCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTg                                                                                                                      <  1:1013014/149‑1 (MQ=255)
                                  ggCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTgg                                                                                                                     >  2:225025/1‑150 (MQ=255)
                                  ggCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTgg                                                                                                                     >  1:1080638/1‑150 (MQ=255)
                                    ccATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTa                                                                                                                   >  2:1281767/1‑150 (MQ=255)
                                     cATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAg                                                                                                                  >  2:486513/1‑150 (MQ=255)
                                       tCACCCGATGACCGTCACTATTTTCTTGTTGCAGGTAGTCCAGGACTGCTGGCTGAGAACGCGTAGCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCa                                                                                                                <  1:102580/150‑1 (MQ=255)
                                       tCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGTAATGCGGGCGGCTTTTGGTAGCa                                                                                                                <  2:1221977/150‑1 (MQ=255)
                                        cACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCAt                                                                                                               >  2:490581/1‑150 (MQ=255)
                                        cACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCAt                                                                                                               >  2:771220/1‑150 (MQ=255)
                                          cccGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGt                                                                                                             >  2:1262252/1‑150 (MQ=255)
                                             gATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTAt                                                                                                          <  1:382364/150‑1 (MQ=255)
                                              aTGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTAtt                                                                                                         <  2:1579067/150‑1 (MQ=255)
                                                gACCGTAACTATTTTCTTGTTGCCGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGt                                                                                                       <  1:627030/150‑1 (MQ=255)
                                                  ccGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttc                                                                                                     >  1:1182682/1‑150 (MQ=255)
                                                  ccGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttc                                                                                                     <  1:757392/150‑1 (MQ=255)
                                                   cGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttct                                                                                                    >  2:1286830/1‑150 (MQ=255)
                                                   cGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttct                                                                                                    <  2:1226507/150‑1 (MQ=255)
                                                   cGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttct                                                                                                    <  2:539525/150‑1 (MQ=255)
                                                      aaCTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttctttc                                                                                                 <  2:1535414/150‑1 (MQ=255)
                                                        cTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTg                                                                                               >  2:366198/1‑150 (MQ=255)
                                                            tttCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTg                                                                                           <  2:1025269/150‑1 (MQ=255)
                                                            tttCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTg                                                                                           <  2:960951/150‑1 (MQ=255)
                                                            tttCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGGCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTg                                                                                           <  2:1643333/150‑1 (MQ=255)
                                                             ttCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGa                                                                                          <  1:1672710/150‑1 (MQ=255)
                                                                  gttgCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACAt                                                                                     <  1:1233136/150‑1 (MQ=255)
                                                                  gttgCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACAt                                                                                     <  1:1227058/150‑1 (MQ=255)
                                                                    tgCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCt                                                                                   >  1:231063/1‑150 (MQ=255)
                                                                     gCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCtt                                                                                  <  2:333802/150‑1 (MQ=255)
                                                                     gCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCCTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCtt                                                                                  <  2:612876/150‑1 (MQ=255)
                                                                      cAGTTAGTCCACGACTTCTGGCTGAGTACGCGGATCGTCCCGCACCGAGTAGTAATTATCGATGAGCGATTCGCAATGAAGCGTCAAGGCGTGGGGAATGCGGGCGGCTTTTGGAAGCATGTTATTGTTGTTTCTGGTTGAAACATCTta                                                                                 <  2:1363732/150‑1 (MQ=255)
                                                                      cAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTta                                                                                 <  2:1639655/150‑1 (MQ=255)
                                                                      cAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTta                                                                                 <  2:1363729/150‑1 (MQ=255)
                                                                      cAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTAGCGATGCGCGTATCGCCATGAGGCGTCATGGCGGGGGGAATGCGGGCGGCTTTTGGGAGCATGTTATTGTTCGTTCTGGTTGTAACATGttt                                                                                 >  1:1434089/1‑149 (MQ=255)
                                                                        ggTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTtata                                                                               >  2:1432789/1‑150 (MQ=255)
                                                                        ggTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCGTTTGGTAGCATGGTATTGTTCGTTCTGGTTGAAACATCTtata                                                                               >  1:1235163/1‑150 (MQ=255)
                                                                         gTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATaa                                                                              >  1:831125/1‑150 (MQ=255)
                                                                          tAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAg                                                                             <  2:383247/150‑1 (MQ=255)
                                                                            gTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGTCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAggg                                                                           <  1:470980/150‑1 (MQ=255)
                                                                            gTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAggg                                                                           <  1:524938/150‑1 (MQ=255)
                                                                              ccAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCGGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTg                                                                         >  1:1481243/1‑150 (MQ=255)
                                                                              ccAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTg                                                                         >  2:853829/1‑150 (MQ=255)
                                                                                aGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGc                                                                       <  1:27215/150‑1 (MQ=255)
                                                                                 ggACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCa                                                                      <  1:1511047/150‑1 (MQ=255)
                                                                                    cTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCaaaa                                                                   >  2:597316/1‑150 (MQ=255)
                                                                                    cTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCaaaa                                                                   >  2:667782/1‑150 (MQ=255)
                                                                                     ttCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAAc                                                                  <  1:1417483/150‑1 (MQ=255)
                                                                                     ttCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAAc                                                                  >  1:1460439/1‑150 (MQ=255)
                                                                                     ttCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAAc                                                                  <  1:464237/150‑1 (MQ=255)
                                                                                           cTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCg                                                            >  1:291531/1‑150 (MQ=255)
                                                                                              agaACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCgggg                                                         >  1:639442/1‑150 (MQ=255)
                                                                                              agaACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTATTTCTGGTTGAAACATCTTAAAAGGGTGGCAAAACTCACCgggg                                                         >  1:1134746/1‑150 (MQ=255)
                                                                                               gaACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGa                                                        >  1:1402095/1‑150 (MQ=255)
                                                                                                  cgcgTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGc                                                     >  1:8421/1‑150 (MQ=255)
                                                                                                     gtctCGTCCCGCAGCCAGAAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTAGTGTTCTTTCTGGTTGAAACATCTTATAAGCGTGGCAAAACTCACCGGGGATGCAAg                                                  <  2:1168246/147‑1 (MQ=255)
                                                                                                      tATCGTCCCGAAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGAGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGAAAAACTCACCGGGGATGAAAGc                                                 >  1:1523805/1‑150 (MQ=255)
                                                                                                       aTCGTCCCGCAGCCAGTCGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCGTTTGGTAGCATGTTATTGTTCTTTCTGGGTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCg                                                <  1:873798/150‑1 (MQ=255)
                                                                                                       aTCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGTCAAAACTAACCGGGGATGCAAGCg                                                >  2:1137784/1‑150 (MQ=255)
                                                                                                          gTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCAGGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGTTGGCAAAACTCACCGGGGATGCAAGCGaaa                                             >  1:293198/1‑150 (MQ=255)
                                                                                                           tCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAAc                                            <  1:1053306/150‑1 (MQ=255)
                                                                                                            cccGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACCTCTTATAAGGGTGGCAAAACTCACCGGCGATGCAAGCGAAACa                                           >  2:107121/1‑150 (MQ=255)
                                                                                                               gCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGGGTCTTGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAggg                                        <  1:210178/150‑1 (MQ=255)
                                                                                                                cAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCTTGGCGTGGGGAATGCGAGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAgggg                                       <  1:80720/150‑1 (MQ=255)
                                                                                                                    cAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTc                                   >  1:890260/1‑150 (MQ=255)
                                                                                                                          taATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCt                             >  1:1590866/1‑150 (MQ=255)
                                                                                                                            aTTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAACCTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTa                           >  2:446437/1‑150 (MQ=255)
                                                                                                                             ttATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAg                          <  2:1080638/150‑1 (MQ=255)
                                                                                                                             ttATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAg                          <  2:890260/150‑1 (MQ=255)
                                                                                                                              tATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAga                         >  2:1181652/1‑150 (MQ=255)
                                                                                                                                  gATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAgatgat                     >  1:383208/1‑150 (MQ=255)
                                                                                                                                      cgcgTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTCATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACa                 >  1:969394/1‑150 (MQ=255)
                                                                                                                                         cccacGCCATGACGCTTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGt              >  2:533281/5‑150 (MQ=255)
                                                                                                                                          ccacGCCATGACGCTTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTa             >  2:495136/4‑150 (MQ=255)
                                                                                                                                           cacGCCATGACGCTTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTat            >  2:836469/3‑149 (MQ=255)
                                                                                                                                           cacGCCATGACGCTTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTaa            >  1:1147397/3‑150 (MQ=255)
                                                                                                                                           aTCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTaa            <  2:12103/150‑1 (MQ=255)
                                                                                                                                              gCCATGAAGCGGCATGGCGTGGGGAATGAGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAATACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATgg         <  1:302524/150‑1 (MQ=255)
                                                                                                                                                  tGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGcgcg     >  2:180842/1‑150 (MQ=255)
                                                                                                                                                     aGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTGCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGat  >  2:812325/1‑150 (MQ=255)
                                                                                                                                                     aGCGTCATGGCGTGGGGAATGCGCGCGGCTTTTGGAAGCATGTTATTGTTCTTTCTGGTTGAACCATCTTATAAGGGGGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGat  <  1:225025/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGAT  >  NC_000913/1928650‑1928948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: