Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,944,233 G→T 9.0% intergenic (+34/+113) znuB → / ← ruvB zinc ABC transporter permease/ATP‑dependent DNA helicase, component of RuvABC resolvasome

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,944,2330GT 9.0% 176.9 / 5.1 66intergenic (+34/+113)znuB/ruvBzinc ABC transporter permease/ATP‑dependent DNA helicase, component of RuvABC resolvasome
Reads supporting (aligned to +/- strand):  ref base G (36/24);  new base T (2/4);  total (38/28)
Fisher's exact test for biased strand distribution p-value = 3.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

AGTGACTGGCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGAT  >  NC_000913/1944085‑1944380
                                                                                                                                                    |                                                                                                                                                   
aGTGACTGGCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGa                                                                                                                                                    >  1:1631855/1‑150 (MQ=255)
  tGACTGGCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAgc                                                                                                                                                  <  1:1412249/150‑1 (MQ=255)
     cTGGCGGTTTAACCTTTTCCGCGGTTTACGATAGGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGcc                                                                                                                                               <  2:1514090/150‑1 (MQ=255)
        gCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTa                                                                                                                                            >  1:668972/1‑150 (MQ=255)
          ggtgtcaCCTTTTCAGCGGTGTCCGATACGCCGGCGGGGACGCCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCCGTCTGATGCACAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATc                                                                                                                                          <  1:258336/144‑1 (MQ=255)
             ttAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGa                                                                                                                                       >  1:676798/1‑150 (MQ=255)
              tAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGAc                                                                                                                                      <  2:1377528/150‑1 (MQ=255)
                 ccTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATAAGGCGCTCGCGCCGCATCCGAcaaa                                                                                                                                   <  1:1383922/150‑4 (MQ=255)
                  cTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGGTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGACCACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTAc                                                                                                                                  >  2:1119522/1‑150 (MQ=255)
                   ttttCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTCTGTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATAAGGCGCTCGCGCCGCATCCGAcaaatg                                                                                                                                 <  1:845302/150‑6 (MQ=255)
                   ttttCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATAAGGCGCTCGCGCCGCATCCGAcaaatg                                                                                                                                 <  1:572980/150‑6 (MQ=255)
                   ttttCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATAAGGCGCTCGCGCCGCATCCGAc                                                                                                                                      <  1:1328602/145‑1 (MQ=255)
                   ttttCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATAAGGCGCTCGCGCCGCATCCGAc                                                                                                                                      >  2:1328602/1‑145 (MQ=255)
                         gcgGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCgg                                                                                                                           <  1:445869/150‑1 (MQ=255)
                            gTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGAAGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGCAACCTGCCCGAGCGCCTTATCCGACCTCCGGATCGGTAt                                                                                                                        >  2:864744/1‑150 (MQ=255)
                                aCGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATTAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATctct                                                                                                                    <  2:195918/150‑1 (MQ=255)
                                  gATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTgg                                                                                                                  >  2:525715/1‑150 (MQ=255)
                                   aTACGCCGTCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGGCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGt                                                                                                                 <  2:878221/150‑1 (MQ=255)
                                   aTACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGt                                                                                                                 >  2:1140509/1‑150 (MQ=255)
                                       gCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTc                                                                                                             >  1:1001198/1‑150 (MQ=255)
                                        gcggcggGGCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTcc                                                                                                            <  2:1392270/149‑1 (MQ=255)
                                        ccGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGTACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTcc                                                                                                            <  2:290259/150‑1 (MQ=255)
                                              ggTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTaa                                                                                                      >  2:1706733/1‑150 (MQ=255)
                                                 ccGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTATTCTATCGCTGAACACATTTGTCGGAGGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGAc                                                                                                   <  1:1074690/150‑1 (MQ=255)
                                                    tCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGAcgcg                                                                                                <  1:1429707/150‑1 (MQ=255)
                                                      ggtggtGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGTATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGaa                                                                                              <  1:1584934/150‑1 (MQ=255)
                                                        tggtgCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACa                                                                                            >  2:1430717/1‑150 (MQ=255)
                                                               aTGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGc                                                                                     >  2:1698711/1‑150 (MQ=255)
                                                                     ggCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAgg                                                                               <  2:1479605/150‑1 (MQ=255)
                                                                      gCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGc                                                                              <  2:472316/150‑1 (MQ=255)
                                                                          tGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAAAACATTTGTCGGATTCGGCGCGAGCGCCTTATCCGACCTACGGTACGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCataa                                                                          >  1:198582/1‑149 (MQ=255)
                                                                           gTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATAtt                                                                         >  1:1660242/1‑150 (MQ=255)
                                                                           gTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATAtt                                                                         >  1:1666743/1‑150 (MQ=255)
                                                                             tatttattCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTTTCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGc                                                                       >  1:1175951/1‑150 (MQ=255)
                                                                             tatttattCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGAGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGc                                                                       >  1:36549/1‑150 (MQ=255)
                                                                               tttattCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCa                                                                     <  2:1432908/150‑1 (MQ=255)
                                                                               tttattCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACATACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATTTTGCCa                                                                     <  2:1703980/150‑1 (MQ=255)
                                                                                        aGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGAt                                                            >  2:1126575/1‑150 (MQ=255)
                                                                                            tgatgaAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGTTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATgcgg                                                        >  2:1538951/1‑150 (MQ=255)
                                                                                            tgatgaAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATgcgg                                                        >  1:1282695/1‑150 (MQ=255)
                                                                                            tgatgaAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATgcgg                                                        <  2:1728218/150‑1 (MQ=255)
                                                                                             gatgaAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATgcggc                                                       >  1:633142/1‑150 (MQ=255)
                                                                                              atgaAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGCACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATgcggcg                                                      >  2:1647709/1‑150 (MQ=255)
                                                                                              atgaAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATgcggcg                                                      >  1:1200282/1‑150 (MQ=255)
                                                                                               tgaAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGgcgc                                                     >  1:1204860/1‑150 (MQ=255)
                                                                                                 aaaaaGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCgcga                                                   >  2:138937/1‑150 (MQ=255)
                                                                                                  aaaaGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCgcgag                                                  >  2:77700/1‑150 (MQ=255)
                                                                                                        aGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCa                                            >  2:1498585/1‑150 (MQ=255)
                                                                                                        aGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCGGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGCCCa                                            >  2:1281771/1‑150 (MQ=255)
                                                                                                         ggCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCaa                                           >  1:626279/1‑150 (MQ=255)
                                                                                                            cAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATcc                                        <  2:1480864/150‑1 (MQ=255)
                                                                                                               cTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGAc                                     >  1:1259699/1‑150 (MQ=255)
                                                                                                                 aaTCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATACGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGCGCGACCAATCCGACtt                                   >  1:52801/1‑150 (MQ=255)
                                                                                                                   tCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTAc                                 >  1:1724504/1‑150 (MQ=255)
                                                                                                                    cTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGTGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACg                                >  2:944530/1‑150 (MQ=255)
                                                                                                                     tATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACgg                               <  1:999126/150‑1 (MQ=255)
                                                                                                                      aTCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTCAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGCGCGCCCAATCCGACGTACGGc                              >  1:206854/1‑150 (MQ=255)
                                                                                                                             aaCACATTTGTCGGATGCGGCGCGAGCGCCGTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTg                       <  1:851268/150‑1 (MQ=255)
                                                                                                                                acaTTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCATGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAAGCCGCCTGGCGGCATTTCTggcg                    >  1:213782/1‑150 (MQ=38)
                                                                                                                                  gtaggTCGGATAAGGCGCTCGCGCCGCATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTggcggc                  >  2:691871/5‑150 (MQ=39)
                                                                                                                                   tttGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGgcggcg                 <  2:1722487/150‑1 (MQ=255)
                                                                                                                                   tttGTCGGAGGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGgcggcg                 <  2:41523/150‑1 (MQ=255)
                                                                                                                                       tCGGATGCGGCGCGAGCGCCGTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTAt             <  2:1355882/150‑1 (MQ=255)
                                                                                                                                         ggATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGc           <  2:1197623/150‑1 (MQ=255)
                                                                                                                                              cggcgCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAg      >  1:894564/1‑150 (MQ=255)
                                                                                                                                                gcgcGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTAGGCCAAAGTg    >  1:1176946/1‑150 (MQ=255)
                                                                                                                                                  gcgAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGAt  >  2:631334/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AGTGACTGGCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGAT  >  NC_000913/1944085‑1944380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: