Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,944,241 T→C 8.7% intergenic (+42/+105) znuB → / ← ruvB zinc ABC transporter permease/ATP‑dependent DNA helicase, component of RuvABC resolvasome

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,944,2410TC 8.7% 189.7 / 8.0 69intergenic (+42/+105)znuB/ruvBzinc ABC transporter permease/ATP‑dependent DNA helicase, component of RuvABC resolvasome
Reads supporting (aligned to +/- strand):  ref base T (41/22);  new base C (2/4);  total (43/26)
Fisher's exact test for biased strand distribution p-value = 1.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.69e-01

GCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCG  >  NC_000913/1944093‑1944390
                                                                                                                                                    |                                                                                                                                                     
gCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTa                                                                                                                                                      >  1:668972/1‑150 (MQ=255)
  ggtgtcaCCTTTTCAGCGGTGTCCGATACGCCGGCGGGGACGCCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCCGTCTGATGCACAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATc                                                                                                                                                    <  1:258336/144‑1 (MQ=255)
     ttAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGa                                                                                                                                                 >  1:676798/1‑150 (MQ=255)
      tAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGAc                                                                                                                                                <  2:1377528/150‑1 (MQ=255)
         ccTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATAAGGCGCTCGCGCCGCATCCGAcaaa                                                                                                                                             <  1:1383922/150‑4 (MQ=255)
          cTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGGTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGACCACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTAc                                                                                                                                            >  2:1119522/1‑150 (MQ=255)
           ttttCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTCTGTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATAAGGCGCTCGCGCCGCATCCGAcaaatg                                                                                                                                           <  1:845302/150‑6 (MQ=255)
           ttttCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATAAGGCGCTCGCGCCGCATCCGAcaaatg                                                                                                                                           <  1:572980/150‑6 (MQ=255)
           ttttCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATAAGGCGCTCGCGCCGCATCCGAc                                                                                                                                                <  1:1328602/145‑1 (MQ=255)
           ttttCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATAAGGCGCTCGCGCCGCATCCGAc                                                                                                                                                >  2:1328602/1‑145 (MQ=255)
                 gcgGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCgg                                                                                                                                     <  1:445869/150‑1 (MQ=255)
                    gTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGAAGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGCAACCTGCCCGAGCGCCTTATCCGACCTCCGGATCGGTAt                                                                                                                                  >  2:864744/1‑150 (MQ=255)
                        aCGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATTAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATctct                                                                                                                              <  2:195918/150‑1 (MQ=255)
                          gATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTgg                                                                                                                            >  2:525715/1‑150 (MQ=255)
                           aTACGCCGTCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGGCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGt                                                                                                                           <  2:878221/150‑1 (MQ=255)
                           aTACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGt                                                                                                                           >  2:1140509/1‑150 (MQ=255)
                               gCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTc                                                                                                                       >  1:1001198/1‑150 (MQ=255)
                                gcggcggGGCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTcc                                                                                                                      <  2:1392270/149‑1 (MQ=255)
                                ccGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGTACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTcc                                                                                                                      <  2:290259/150‑1 (MQ=255)
                                      ggTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTaa                                                                                                                >  2:1706733/1‑150 (MQ=255)
                                         ccGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTATTCTATCGCTGAACACATTTGTCGGAGGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGAc                                                                                                             <  1:1074690/150‑1 (MQ=255)
                                            tCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGAcgcg                                                                                                          <  1:1429707/150‑1 (MQ=255)
                                              ggtggtGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGTATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGaa                                                                                                        <  1:1584934/150‑1 (MQ=255)
                                                tggtgCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACa                                                                                                      >  2:1430717/1‑150 (MQ=255)
                                                       aTGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGc                                                                                               >  2:1698711/1‑150 (MQ=255)
                                                             ggCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAgg                                                                                         <  2:1479605/150‑1 (MQ=255)
                                                              gCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGc                                                                                        <  2:472316/150‑1 (MQ=255)
                                                                  tGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAAAACATTTGTCGGATTCGGCGCGAGCGCCTTATCCGACCTACGGTACGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCataa                                                                                    >  1:198582/1‑149 (MQ=255)
                                                                   gTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATAtt                                                                                   >  1:1666743/1‑150 (MQ=255)
                                                                   gTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATAtt                                                                                   >  1:1660242/1‑150 (MQ=255)
                                                                     tatttattCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTTTCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGc                                                                                 >  1:1175951/1‑150 (MQ=255)
                                                                     tatttattCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGAGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGc                                                                                 >  1:36549/1‑150 (MQ=255)
                                                                       tttattCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCa                                                                               <  2:1432908/150‑1 (MQ=255)
                                                                       tttattCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACATACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATTTTGCCa                                                                               <  2:1703980/150‑1 (MQ=255)
                                                                                aGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGAt                                                                      >  2:1126575/1‑150 (MQ=255)
                                                                                    tgatgaAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGTTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATgcgg                                                                  >  2:1538951/1‑150 (MQ=255)
                                                                                    tgatgaAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATgcgg                                                                  >  1:1282695/1‑150 (MQ=255)
                                                                                    tgatgaAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATgcgg                                                                  <  2:1728218/150‑1 (MQ=255)
                                                                                     gatgaAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATgcggc                                                                 >  1:633142/1‑150 (MQ=255)
                                                                                      atgaAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGCACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATgcggcg                                                                >  2:1647709/1‑150 (MQ=255)
                                                                                      atgaAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATgcggcg                                                                >  1:1200282/1‑150 (MQ=255)
                                                                                       tgaAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGgcgc                                                               >  1:1204860/1‑150 (MQ=255)
                                                                                         aaaaaGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCgcga                                                             >  2:138937/1‑150 (MQ=255)
                                                                                          aaaaGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCgcgag                                                            >  2:77700/1‑150 (MQ=255)
                                                                                                aGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCa                                                      >  2:1498585/1‑150 (MQ=255)
                                                                                                aGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCGGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGCCCa                                                      >  2:1281771/1‑150 (MQ=255)
                                                                                                 ggCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCaa                                                     >  1:626279/1‑150 (MQ=255)
                                                                                                    cAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATcc                                                  <  2:1480864/150‑1 (MQ=255)
                                                                                                       cTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGAc                                               >  1:1259699/1‑150 (MQ=255)
                                                                                                         aaTCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATACGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGCGCGACCAATCCGACtt                                             >  1:52801/1‑150 (MQ=255)
                                                                                                           tCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTAc                                           >  1:1724504/1‑150 (MQ=255)
                                                                                                            cTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGTGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACg                                          >  2:944530/1‑150 (MQ=255)
                                                                                                             tATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACgg                                         <  1:999126/150‑1 (MQ=255)
                                                                                                              aTCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTCAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGCGCGCCCAATCCGACGTACGGc                                        >  1:206854/1‑150 (MQ=255)
                                                                                                                     aaCACATTTGTCGGATGCGGCGCGAGCGCCGTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTg                                 <  1:851268/150‑1 (MQ=255)
                                                                                                                        acaTTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCATGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAAGCCGCCTGGCGGCATTTCTggcg                              >  1:213782/1‑150 (MQ=38)
                                                                                                                          gtaggTCGGATAAGGCGCTCGCGCCGCATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTggcggc                            >  2:691871/5‑150 (MQ=39)
                                                                                                                           tttGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGgcggcg                           <  2:1722487/150‑1 (MQ=255)
                                                                                                                           tttGTCGGAGGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGgcggcg                           <  2:41523/150‑1 (MQ=255)
                                                                                                                               tCGGATGCGGCGCGAGCGCCGTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTAt                       <  2:1355882/150‑1 (MQ=255)
                                                                                                                                 ggATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGc                     <  2:1197623/150‑1 (MQ=255)
                                                                                                                                      cggcgCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAg                >  1:894564/1‑150 (MQ=255)
                                                                                                                                        gcgcGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTAGGCCAAAGTg              >  1:1176946/1‑150 (MQ=255)
                                                                                                                                          gcgAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGAt            >  2:631334/1‑150 (MQ=255)
                                                                                                                                             aGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTcc         >  1:28680/1‑150 (MQ=255)
                                                                                                                                             aGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTcc         >  1:118167/1‑150 (MQ=255)
                                                                                                                                                gcCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACg      >  2:977834/1‑150 (MQ=255)
                                                                                                                                                 ccTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCCCGc     >  2:1693180/1‑150 (MQ=255)
                                                                                                                                                  cTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGcc    >  2:33715/1‑150 (MQ=255)
                                                                                                                                                    tATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCcg  >  2:1603417/1‑150 (MQ=255)
                                                                                                                                                    tATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCcg  >  1:895326/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCG  >  NC_000913/1944093‑1944390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: