Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,948,179 T→A 9.1% intergenic (‑28/+1) yebC ← / ← nudB UPF0082 family protein/dihydroneopterin triphosphate pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,948,1790TA 9.1% 276.7 / 12.8 104intergenic (‑28/+1)yebC/nudBUPF0082 family protein/dihydroneopterin triphosphate pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base T (46/48);  new base A (6/3);  total (52/52)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

Only 100 of 105 total aligned reads displayed.

CCACCGCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAGACAGAAC  >  NC_000913/1948030‑1948327
                                                                                                                                                     |                                                                                                                                                    
ccACCGCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGt                                                                                                                                                      >  2:1409444/1‑150 (MQ=255)
      cccAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGc                                                                                                                                                <  1:1101383/150‑1 (MQ=255)
      cccAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGc                                                                                                                                                <  1:239248/150‑1 (MQ=255)
          gCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCg                                                                                                                                            >  2:361582/1‑150 (MQ=255)
          gCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGAACAGGCAggg                                                                                                                                            <  2:480746/150‑3 (MQ=255)
          gCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGAACAGGCAggg                                                                                                                                            <  1:366390/150‑3 (MQ=255)
            ttAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGtt                                                                                                                                          >  1:1440174/1‑150 (MQ=255)
            ttAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGAACAGGCAgggct                                                                                                                                          <  2:944892/150‑5 (MQ=255)
              aGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTaa                                                                                                                                        <  2:1468741/150‑1 (MQ=255)
               cccGCGCTTACCACCTCACCAATGATATTAGTGAAGATTTTACCGCGCTTAGAAACCTGCGCAGCATTAGGATGTATGGTGTTGTCCGAATTACTATTACCTGCCAGAAAAATATCTAGAGATTGCCCTGCCTGTTCGGGAAGCGTTTAt                                                                                                                                       >  2:1620670/2‑150 (MQ=255)
                 cgcgGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTa                                                                                                                                     <  1:1668956/150‑1 (MQ=255)
                 cgcgGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTa                                                                                                                                     <  2:478234/150‑1 (MQ=255)
                       tACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACt                                                                                                                               >  1:488007/1‑150 (MQ=255)
                           aGCTCACGAATGATTTTAGTGTAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTc                                                                                                                           <  2:527778/150‑1 (MQ=255)
                            gCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCa                                                                                                                          >  2:447424/1‑150 (MQ=255)
                               cACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCGTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCAATAAAAATATCTCCAGATAGCCCTGACTGTTAAGGAAGCGTTAATTAAAAAATGTTCAATc                                                                                                                       >  2:266054/1‑150 (MQ=255)
                                  gAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGcc                                                                                                                    >  1:160499/1‑150 (MQ=255)
                                  gAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGcc                                                                                                                    >  1:422746/1‑150 (MQ=255)
                                  gAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGcc                                                                                                                    <  2:1372797/150‑1 (MQ=255)
                                  gAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGcc                                                                                                                    <  2:486782/150‑1 (MQ=255)
                                  gAATGATTTTAGGGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGcc                                                                                                                    <  2:151130/150‑1 (MQ=255)
                                   aaTGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCt                                                                                                                   <  1:1027450/150‑1 (MQ=255)
                                    aTGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTg                                                                                                                  <  2:1209670/150‑1 (MQ=255)
                                     tGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTGCAACCggcagg                                                                                                                 <  1:1534851/150‑6 (MQ=255)
                                       aTTTTAGTGAAGATTTTACCGCGCTGAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCg                                                                                                               >  1:175500/1‑150 (MQ=255)
                                         tttAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGt                                                                                                             <  1:1387683/150‑1 (MQ=255)
                                         tttAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGt                                                                                                             <  1:1203073/150‑1 (MQ=255)
                                            aGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTTAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGc                                                                                                          <  1:407561/150‑1 (MQ=255)
                                            aGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTTTTCAATCGCCTGCAGGTTGc                                                                                                          >  2:136463/1‑150 (MQ=255)
                                             gTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCt                                                                                                         >  1:74006/1‑150 (MQ=255)
                                              tGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCGGCCGGTTGCTc                                                                                                        <  1:924406/150‑1 (MQ=255)
                                              tGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCACTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTc                                                                                                        >  2:395922/1‑150 (MQ=255)
                                               gAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTcc                                                                                                       >  2:947303/1‑150 (MQ=255)
                                               gAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTcc                                                                                                       >  2:437648/1‑150 (MQ=255)
                                               gAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTcc                                                                                                       >  2:64067/1‑150 (MQ=255)
                                                aaGTTTTTACCGGGCTTAGCATCCTGCGCAGCTTTACGTTGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCa                                                                                                      <  1:1133805/150‑1 (MQ=255)
                                                  gATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAgg                                                                                                    <  1:515431/150‑1 (MQ=255)
                                                  gATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAgg                                                                                                    <  2:1646977/150‑1 (MQ=255)
                                                   aTTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGa                                                                                                   >  1:550310/1‑150 (MQ=255)
                                                      ttACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACtt                                                                                                >  1:771032/1‑150 (MQ=255)
                                                         ccGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGt                                                                                             >  2:1519093/1‑150 (MQ=255)
                                                           gcgcTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGa                                                                                           <  1:1340275/150‑1 (MQ=255)
                                                             gcTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAgc                                                                                         <  2:1206506/150‑1 (MQ=255)
                                                              cTTAGCATCCTGCGGAGCTTTACGATGTCTGGTGATGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAgcg                                                                                        <  1:108460/150‑1 (MQ=255)
                                                                    aTCCTGCGCAGCTTTACGATGTCTGGTGTTGACCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGcgccgccg                                                                                  >  2:469307/1‑150 (MQ=255)
                                                                     tCCTGCGCAGCTTTACGCAGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGGTAAATACAAACTGTTCACTCGCCTGCCGGTTGCTCCAGGACTTAGTGAGcgccgccgc                                                                                 <  2:1518867/150‑1 (MQ=255)
                                                                         gcgcAGCTTTACGATGTCTGGGGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCgcagca                                                                             <  2:920797/150‑1 (MQ=255)
                                                                            cAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGGCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGgc                                                                          <  2:131118/150‑1 (MQ=255)
                                                                              gCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGgcgc                                                                        >  1:1478816/1‑150 (MQ=255)
                                                                              gCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGgcgc                                                                        >  1:604304/1‑150 (MQ=255)
                                                                               cTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCa                                                                       >  1:1344854/1‑150 (MQ=255)
                                                                                  tACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCa                                                                    <  1:572806/150‑1 (MQ=255)
                                                                                    cGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAg                                                                  <  1:1610723/150‑1 (MQ=255)
                                                                                     gATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGc                                                                 >  2:1105562/1‑150 (MQ=255)
                                                                                     gATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGc                                                                 <  1:158033/150‑1 (MQ=255)
                                                                                      aTGTCTGGTGGTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTACAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGcc                                                                <  2:119916/150‑1 (MQ=255)
                                                                                        gTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCAc                                                              <  2:1019390/150‑1 (MQ=255)
                                                                                          cTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCTAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACtt                                                            >  2:264617/1‑150 (MQ=255)
                                                                                           tGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTg                                                           >  1:1099748/1‑150 (MQ=255)
                                                                                             gtgtTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTa                                                         <  1:1409444/150‑1 (MQ=255)
                                                                                              tgtTGGCCCATTTACTATGACCGGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTaa                                                        >  2:349783/1‑150 (MQ=255)
                                                                                               gtTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAg                                                       <  1:64067/150‑1 (MQ=255)
                                                                                                   gCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAg                                                   >  1:1063175/1‑150 (MQ=255)
                                                                                                   gCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAg                                                   >  1:535583/1‑150 (MQ=255)
                                                                                                   gCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACAGTTCAATCGCCTGCAGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCCGTCGCATCAAGCCACTTGTAAGCCAg                                                   >  1:101990/1‑150 (MQ=255)
                                                                                                    cccATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGa                                                  >  1:931241/1‑150 (MQ=255)
                                                                                                     ccATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGAt                                                 >  1:554709/1‑150 (MQ=255)
                                                                                                      cATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATg                                                <  2:488007/150‑1 (MQ=255)
                                                                                                      cATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATg                                                >  2:1133341/1‑150 (MQ=255)
                                                                                                      cATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATg                                                <  2:748343/150‑1 (MQ=255)
                                                                                                           aCTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAg                                           <  2:771032/150‑1 (MQ=255)
                                                                                                             tATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTg                                         <  2:1297647/150‑1 (MQ=255)
                                                                                                                gACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGaaa                                      <  1:447424/150‑1 (MQ=255)
                                                                                                                  ccTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAAc                                    <  1:361582/150‑1 (MQ=255)
                                                                                                                   cTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCTCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACg                                   >  1:1497541/1‑150 (MQ=255)
                                                                                                                   cTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACg                                   >  2:1070705/1‑150 (MQ=255)
                                                                                                                    tGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGcc                                                                <  2:1514791/120‑1 (MQ=255)
                                                                                                                    tGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGcc                                                                >  1:1514791/1‑120 (MQ=255)
                                                                                                                     gCCATAAAAATATCTCCAGATAGCCCTGCCTGCTCAGGCAGCGTTAATAACAAACTGTTCAATCGCCTGCCGGTGGCTCCAGGACTTAGTGAGCGCCGCCTCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGAt                                 <  1:118396/150‑1 (MQ=255)
                                                                                                                       cATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCt                               >  1:1310449/1‑150 (MQ=255)
                                                                                                                          aaaaaTATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGcc                            >  2:926127/1‑150 (MQ=255)
                                                                                                                          aaaaaTATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGcc                            <  2:554709/150‑1 (MQ=255)
                                                                                                                                  ccccAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGc                    >  1:770421/3‑150 (MQ=255)
                                                                                                                                   tcCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCACTCGACTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCAGCTCGTGCg                   <  1:1468592/150‑1 (MQ=255)
                                                                                                                                   tcCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCg                   >  2:535244/1‑150 (MQ=255)
                                                                                                                                    ccAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCAGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCgc                  >  1:1015429/1‑149 (MQ=255)
                                                                                                                                        aTAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGGTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAgc              <  1:859419/150‑1 (MQ=255)
                                                                                                                                         tAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAgcg             >  2:1278170/1‑150 (MQ=255)
                                                                                                                                          aacgctgcctgAACAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGCAgcgc            >  2:1542973/5‑150 (MQ=255)
                                                                                                                                          aacgctgcctgAACAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAgcgc            >  2:471480/5‑150 (MQ=255)
                                                                                                                                          aacgctgcctgAACAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAgcgc            >  2:445328/5‑150 (MQ=255)
                                                                                                                                          aacgctgcctgAACAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAgcgc            >  2:1602311/5‑150 (MQ=255)
                                                                                                                                          aacgctgcctgAACAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAgcgc            >  2:1070862/5‑150 (MQ=255)
                                                                                                                                           acgctgcctgAACAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCa           >  1:1432859/4‑150 (MQ=255)
                                                                                                                                            cccTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGTGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCaa          <  1:857038/150‑1 (MQ=255)
                                                                                                                                             cctgcctgTTCAGTCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAg         <  2:1020069/150‑1 (MQ=255)
                                                                                                                                             cctgcctgTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAg         >  1:469981/1‑150 (MQ=255)
                                                                                                                                             cctgcctgTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAg         >  2:952502/1‑150 (MQ=255)
                                                                                                                                                gcctgTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAGACa      <  1:1519093/150‑1 (MQ=255)
                                                                                                                                                    gTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAGACAGAAc  <  1:1133341/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCACCGCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAGACAGAAC  >  NC_000913/1948030‑1948327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: