Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 240,840 T→G 7.6% intergenic (+24/+19) ivy → / ← fadE inhibitor of c‑type lysozyme, periplasmic/acyl coenzyme A dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913240,8400TG 7.6% 313.1 / 11.7 111intergenic (+24/+19)ivy/fadEinhibitor of c‑type lysozyme, periplasmic/acyl coenzyme A dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (52/49);  new base G (5/4);  total (57/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 113 total aligned reads displayed.

GTCGCAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCT  >  NC_000913/240693‑240988
                                                                                                                                                   |                                                                                                                                                    
gTCGCAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCttt                                                                                                                                                    >  2:736993/1‑150 (MQ=255)
  gtcAAGAGAATCTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATTGTAAAACGTTGTTGTTCGCTGTGTTGACCGGCTGCCTGGAAAAGCATCCGGATGGCTTTAATTTTAAATATTTAGCGGATAAAGAAACGGTGCCTTTCg                                                                                                                                                  <  1:307527/148‑1 (MQ=255)
  cGCAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCg                                                                                                                                                  >  2:1683763/1‑150 (MQ=255)
    cAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGc                                                                                                                                                <  1:193418/150‑1 (MQ=255)
    cAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGc                                                                                                                                                <  2:646163/150‑1 (MQ=255)
    cAAGAGAAACTCACCTGGCTGAATGTGAACGAAGCGCTTTCGATTGATGGTAAAACGGTCTAGTTCTCGGCCTTGACCGGCAGCCTGCAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGAGTATAAAGAAACGGATCCTTTCgag                                                                                                                                                >  2:307527/1‑148 (MQ=255)
     aaGAGAAACTCACCTGGCTGAATGTGCACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCt                                                                                                                                               >  1:246884/1‑150 (MQ=255)
         gaATCTCACCTGGCGGAATGTGAACGAAGCGCTATCGAGTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGt                                                                                                                                           <  2:309954/150‑1 (MQ=255)
         gaAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGt                                                                                                                                           <  2:1098919/150‑1 (MQ=255)
         gaAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGt                                                                                                                                           >  1:896241/1‑150 (MQ=255)
         gaAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGt                                                                                                                                           >  1:134616/1‑150 (MQ=255)
         gaAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGtt                                                                                                                                          <  2:1599091/150‑1 (MQ=255)
          aaaCTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGtt                                                                                                                                          <  2:519597/150‑1 (MQ=255)
          aaaCTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGtt                                                                                                                                          <  2:1185191/150‑1 (MQ=255)
            aCTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCGAAAGGCTCCGtttc                                                                                                                                        <  2:1241384/150‑3 (MQ=255)
             cTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTAtt                                                                                                                                       <  2:903515/150‑1 (MQ=255)
              tCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAACCGGAGCCGAAAGGCTCCGtttctt                                                                                                                                      <  2:813513/150‑5 (MQ=255)
              tCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCGAAAGGCTCCGtttctt                                                                                                                                      <  2:696226/150‑5 (MQ=255)
               cACCTGTCTGAATGTGAACGATGCGCTTTCGATTGATTGTAAAACTGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCa                                                                                                                                     <  2:1629755/150‑1 (MQ=255)
                             tgAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCa                                                                                                                       >  1:554951/1‑150 (MQ=255)
                              gAACGATGCGCTTCCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCaa                                                                                                                      >  2:322016/1‑150 (MQ=255)
                               aaCGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAACTAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAAc                                                                                                                     <  1:1053113/150‑1 (MQ=255)
                                             gATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTtct                                                                                                       <  1:788759/150‑1 (MQ=255)
                                             gATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTtct                                                                                                       >  1:1588420/1‑150 (MQ=255)
                                               ttGATGGTAATACGGTGTTGTTCGCGGCGGTGACCGGCAGCCTGGAAAACCATCCGGAGCGCTTTAATTTTAAATAATTTCCGGATAAAGAAACGGAGCCTTTCGGCGCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTcc                                                                                                     <  1:1339221/150‑1 (MQ=255)
                                                  attgTAAAACGGTGTTGTTCGCGGCTTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGc                                                                                                  <  1:299454/147‑1 (MQ=255)
                                                  aTGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGc                                                                                                  <  1:1683763/150‑1 (MQ=255)
                                                  aTGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTACGCACTTTCTCCGGc                                                                                                  >  1:1709445/1‑150 (MQ=255)
                                                    ggTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGTCTTCAACTTTCCGCACTTTCTCAGGCaa                                                                                                >  1:198606/1‑150 (MQ=255)
                                                     gTAAAACGGAGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAACCCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAAc                                                                                               <  2:1088177/150‑1 (MQ=255)
                                                        aaaCGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAAAAATACGGATGGCTTAAATTTTAAATAATTAGCGGATAAAGAAACGGATCCTATCGGCTCAGTTATTCATTTACGCGGCTTCAACTTACCGCACTTTCTCCGGCAACttt                                                                                            >  1:498178/1‑150 (MQ=255)
                                                          aCGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAACGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTAc                                                                                          <  2:650369/150‑1 (MQ=255)
                                                          aCGGTGTTGTTCGCGGCGTAGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTAc                                                                                          <  2:635514/150‑1 (MQ=255)
                                                           ctgtgtTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTAcc                                                                                         <  2:1522733/148‑1 (MQ=255)
                                                           cGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTAcc                                                                                         <  1:989289/150‑1 (MQ=255)
                                                           cGGGGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCGTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTAcc                                                                                         <  1:1155054/150‑1 (MQ=255)
                                                            ggTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCg                                                                                        >  1:615310/1‑150 (MQ=255)
                                                                tttttCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCtt                                                                                    <  1:1187863/147‑1 (MQ=255)
                                                                ttgttCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCtt                                                                                    <  2:1313812/150‑1 (MQ=255)
                                                                 tgttCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTtc                                                                                   >  1:28383/1‑150 (MQ=255)
                                                                 tgttCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTAAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTtc                                                                                   >  1:1314144/1‑150 (MQ=255)
                                                                 tgttCGCGGCGTTGACCGGCAGCCTGGAAAACCATACGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCTGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTTCCGGATtc                                                                                   >  2:1622359/1‑150 (MQ=255)
                                                                   ttCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTtcgt                                                                                 >  1:1654159/1‑150 (MQ=255)
                                                                    tCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGTATAATGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTtcgtc                                                                                <  1:1410526/150‑1 (MQ=255)
                                                                    tCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTtcgtc                                                                                >  1:1529483/1‑150 (MQ=255)
                                                                       gggcgTTGACCGGCAGCCTGGAAAACGAGCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGcc                                                                             <  1:350837/149‑1 (MQ=255)
                                                                         gcgTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAg                                                                           <  1:617857/150‑1 (MQ=255)
                                                                          cgTTGACCGGCAGCCTGGTAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGc                                                                          >  2:1627306/1‑150 (MQ=255)
                                                                          cgTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGc                                                                          >  1:486492/1‑150 (MQ=255)
                                                                               aCCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGAAAAAGAAACGGAGCCTTACGGCTCCGTTATTCATTTAAGCGGCTTCAACTTTACGCACTTTCACCGGCCACTATACCGGCTTCGTCGCCAGCTCTTc                                                                     >  2:264692/1‑150 (MQ=255)
                                                                                  ggCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCATCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCgg                                                                  >  2:838600/1‑150 (MQ=255)
                                                                                   gCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGa                                                                 <  1:736993/150‑1 (MQ=255)
                                                                                        cTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCaaa                                                            >  1:433250/1‑150 (MQ=255)
                                                                                        cTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCaaa                                                            >  1:1054206/1‑150 (MQ=255)
                                                                                        cTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCaaa                                                            >  1:1622388/1‑150 (MQ=255)
                                                                                         tGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAg                                                           >  2:613480/1‑150 (MQ=255)
                                                                                         tGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAg                                                           >  2:1033365/1‑150 (MQ=255)
                                                                                         tGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAg                                                           <  1:1461317/150‑1 (MQ=255)
                                                                                         tGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTACGCACTTTATCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCaaat                                                           >  1:1273198/1‑149 (MQ=255)
                                                                                         tGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTAATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAg                                                           >  1:823499/1‑150 (MQ=255)
                                                                                               aCCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTTCCGGCTTCGTCGCCAGCTCTTCCGGAGCAAAGtgatca                                                     <  2:1594186/150‑1 (MQ=255)
                                                                                               aCCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGtcatca                                                     >  1:610486/1‑150 (MQ=255)
                                                                                                  aTCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTTTTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACg                                                  >  1:1072906/1‑150 (MQ=255)
                                                                                                  aTCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACg                                                  >  2:1527245/1‑150 (MQ=255)
                                                                                                  aTCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACg                                                  >  1:637102/1‑150 (MQ=255)
                                                                                                    ccGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGtt                                                >  2:1233293/1‑150 (MQ=255)
                                                                                                     cGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTa                                               >  1:583095/1‑150 (MQ=255)
                                                                                                      ggATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTACGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCACTTCCGGATCAAAGTCATCAACGTTaa                                              >  2:361630/1‑150 (MQ=255)
                                                                                                         tGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCTGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATAc                                           <  1:361651/150‑1 (MQ=255)
                                                                                                          ggCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACt                                          <  2:1588420/150‑1 (MQ=255)
                                                                                                           gCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATAATg                                         >  1:1435111/1‑150 (MQ=255)
                                                                                                           gCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACATTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGACATCAACGTTAATACTg                                         >  2:104395/1‑150 (MQ=255)
                                                                                                            cTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTgc                                        >  2:1323470/1‑150 (MQ=255)
                                                                                                            cTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTgc                                        <  2:610486/150‑1 (MQ=255)
                                                                                                             tttAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTgcg                                       <  2:246884/150‑1 (MQ=255)
                                                                                                             tttAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCGTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTgcg                                       <  1:927921/150‑1 (MQ=255)
                                                                                                              ttAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTgcgc                                      <  1:1089561/150‑1 (MQ=255)
                                                                                                                 aTTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCAGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGa                                   >  2:43005/1‑150 (MQ=255)
                                                                                                                    ttAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACgg                                >  2:922005/1‑150 (MQ=255)
                                                                                                                     tAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGc                               <  1:428304/150‑1 (MQ=255)
                                                                                                                       aataatTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCtt                             >  1:1706923/1‑150 (MQ=255)
                                                                                                                         taatTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTttc                           <  2:1031111/150‑1 (MQ=255)
                                                                                                                          aatTAGCGGATAAAGAAACGGCGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTttct                          <  2:1057491/150‑1 (MQ=255)
                                                                                                                          aatTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTGCGGATCAAAGTCATCAACGTTAATTCTGCGCAGACGGCTttct                          >  2:891436/1‑150 (MQ=255)
                                                                                                                          aatTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTttct                          <  1:632434/150‑1 (MQ=255)
                                                                                                                              aGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAg                      <  1:1348771/150‑1 (MQ=255)
                                                                                                                              aGCGGATAAAGAAACGGAGCCATTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAg                      <  1:394183/150‑1 (MQ=255)
                                                                                                                                 ggATAACGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCtt                   <  1:104691/150‑1 (MQ=255)
                                                                                                                                  gATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCttt                  <  2:134616/150‑1 (MQ=255)
                                                                                                                                  gATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCttt                  <  2:896241/150‑1 (MQ=255)
                                                                                                                                  gATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGAACAAAGACATCAACGTTAATACTGCGCTGACGGCTTTATTCAGAttt                  >  2:288429/1‑150 (MQ=255)
                                                                                                                                     tgaataaCGGAGCCGAAAGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCAc               >  1:800571/6‑150 (MQ=255)
                                                                                                                                     tgaataaCGGAGCCGAAAGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCAc               >  1:795167/6‑150 (MQ=255)
                                                                                                                                       aGAAACGGAGCCTTTCGCCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTATACCGGCTTCGTCGCCATCTCTTCCGGATCAAAGTCATCAACGATAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCa             <  1:204651/150‑1 (MQ=255)
                                                                                                                                          aaCGGAGCCGAAAGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCAACAGaa          >  2:1329798/1‑150 (MQ=255)
                                                                                                                                          aaCGGAGCCGAAAGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTATCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCAGATCAAAGTCATCAACGTTAATAATGCGCAGACGGCTTTCTTCCGCTTTCACCAGaa          >  1:1088727/1‑150 (MQ=255)
                                                                                                                                           aCGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTTCCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAAt         <  2:554951/150‑1 (MQ=255)
                                                                                                                                               aGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCg     <  1:1176614/150‑1 (MQ=255)
                                                                                                                                                  cTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCt  <  1:718078/150‑1 (MQ=255)
                                                                                                                                                  cTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCt  >  2:1203379/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GTCGCAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCT  >  NC_000913/240693‑240988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: