Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,982,504 G→A 10.5% intergenic (‑117/+50) otsB ← / ← araH trehalose‑6‑phosphate phosphatase, biosynthetic/L‑arabinose ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,982,5040GA10.5% 268.2 / 15.2 95intergenic (‑117/+50)otsB/araHtrehalose‑6‑phosphate phosphatase, biosynthetic/L‑arabinose ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (35/50);  new base A (4/6);  total (39/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.89e-01

GTTCAGGGGTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATT  >  NC_000913/1982357‑1982652
                                                                                                                                                   |                                                                                                                                                     
gTTCAGGGGTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGc                                                                                                                                                     <  2:382743/150‑1 (MQ=255)
gTTCAGGGGTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGATTTACTGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGACCAGCCATCCGGTATGGc                                                                                                                                                     >  2:918530/1‑150 (MQ=255)
 ttCTGGGGTTTCGGTTATCGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGGTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCg                                                                                                                                                    <  2:155062/150‑1 (MQ=255)
  tCAGGGGTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTGTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCgg                                                                                                                                                   <  2:322179/150‑1 (MQ=255)
       ggTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAATAGCCAACCGCCATAACGGTTGGc                                                                                                                                              <  1:1339936/150‑1 (MQ=255)
       ggTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGc                                                                                                                                              >  2:1668852/1‑150 (MQ=255)
       ggTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGc                                                                                                                                              <  1:1714860/150‑1 (MQ=255)
       ggTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGCCATAACGGTTGGc                                                                                                                                              <  2:437818/150‑1 (MQ=255)
       ggTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGCCATAACGGTTGGc                                                                                                                                              <  1:29741/150‑1 (MQ=255)
        gtatCGGTTAACGGTGCTGTCACCCGGTTCTCCTCCATCTCTTTTCTTATTGTGATGCGCAGAACGCGCATTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCt                                                                                                                                             <  2:751583/147‑1 (MQ=255)
          ttCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGCCATAACGGTTGGCtgt                                                                                                                                           <  2:482124/150‑3 (MQ=255)
           tCGGTTCACGGCTCTGTCACCCGGTGCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATTGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGCCATAACGGTTGGCtgtt                                                                                                                                          <  2:1556408/150‑4 (MQ=255)
           tCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTAtg                                                                                                                                          <  1:588897/150‑1 (MQ=255)
            cGGTTTACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAATAGCCAACCGCCATAACGGTTGGCtgttc                                                                                                                                         <  2:1235507/150‑5 (MQ=255)
            cGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTAtgg                                                                                                                                         >  1:411011/1‑150 (MQ=255)
             tgTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTAtggt                                                                                                                                        >  2:1324285/2‑150 (MQ=255)
             ggTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGTGGTTGGCTAtggt                                                                                                                                        >  2:159418/1‑150 (MQ=255)
              gTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTAtggtg                                                                                                                                       >  2:189217/1‑150 (MQ=255)
               ttAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTAtggtgg                                                                                                                                      <  2:1445001/150‑1 (MQ=255)
               ttAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTAtggtgg                                                                                                                                      <  1:726224/150‑1 (MQ=255)
                  aCGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGCCGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGaa                                                                                                                                   <  1:1005945/150‑1 (MQ=255)
                  aCGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGaa                                                                                                                                   <  2:868843/150‑1 (MQ=255)
                  aCGGTTCAGGCACCCGGTGCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCGTTACAGACATGTAAGTATCGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGACCAGCCAACCGTTATGGCGGTTGGCTATGGTGGGaa                                                                                                                                   <  1:169122/150‑1 (MQ=255)
                     gTTGTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTCTGTTGCGCAGAACGCGCTTTACAGACATGTTAGTATAGACAGTGAGCCGGGGGTCGCCATTTGCAACGTAGTACAGCCAACCGTTATGTCGGTTGGCTATTGTGGGaaaat                                                                                                                                <  2:689507/150‑2 (MQ=255)
                                cGGGTCCCCTCCAACTCATTTCTTATTGTGGTGCCCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAAttg                                                                                                                     <  1:143092/150‑1 (MQ=255)
                                       cctcctTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGa                                                                                                             >  1:817862/1‑150 (MQ=255)
                                        ctcctTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGGCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGaa                                                                                                            >  2:1173616/1‑150 (MQ=255)
                                             tctcTTTTCTTATTGTGTTGCGCAGAACTCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGAAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAg                                                                                                       <  1:522985/150‑1 (MQ=255)
                                             tctcTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAg                                                                                                       <  2:1431544/150‑1 (MQ=255)
                                             tctcTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAg                                                                                                       <  2:1212484/150‑1 (MQ=255)
                                               tctTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCa                                                                                                     <  2:566862/150‑1 (MQ=255)
                                                ctTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCAt                                                                                                    <  2:761589/150‑1 (MQ=255)
                                                ctTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCAt                                                                                                    >  2:618284/1‑150 (MQ=255)
                                                  tttCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCa                                                                                                  >  2:666121/1‑150 (MQ=255)
                                                   ttCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAg                                                                                                 >  1:488622/1‑150 (MQ=255)
                                                    tCTTATTGTGTTGCGCAGAACGCGCTTTACTGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGa                                                                                                <  1:945969/150‑1 (MQ=255)
                                                      ttATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACa                                                                                              >  1:951172/1‑150 (MQ=255)
                                                         ttGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTg                                                                                           <  2:411011/150‑1 (MQ=255)
                                                         ttGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTg                                                                                           <  1:451603/150‑1 (MQ=255)
                                                         ttGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTg                                                                                           <  2:484564/150‑1 (MQ=255)
                                                               tGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTc                                                                                     <  2:975020/150‑1 (MQ=255)
                                                                gcgcTGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCg                                                                                    <  1:28807/150‑1 (MQ=255)
                                                                gcgcAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCg                                                                                    <  2:899816/150‑1 (MQ=255)
                                                                gcgcAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCg                                                                                    >  1:1527354/1‑150 (MQ=255)
                                                                   cAGAACGCGCTTTACAGACATGTACGTATCGACAGTGTGCCGGGGGTCACCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAACCGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCtt                                                                                 <  2:951121/150‑1 (MQ=255)
                                                                       aCGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCATCGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTg                                                                             <  2:149503/150‑1 (MQ=255)
                                                                       aCGCGATTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCAATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTg                                                                             <  2:1255991/150‑1 (MQ=255)
                                                                          cgcTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCtt                                                                          <  2:601077/150‑1 (MQ=255)
                                                                           gcTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGTGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTg                                                                         <  1:159418/150‑1 (MQ=255)
                                                                           gcTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTg                                                                         >  2:1712815/1‑150 (MQ=255)
                                                                                aCAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGCGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTACGCTTTTTGCTTGTAACg                                                                    >  2:366578/1‑150 (MQ=255)
                                                                                 cAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACgg                                                                   <  1:1094597/150‑1 (MQ=255)
                                                                                 cAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCAGTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCTGACAGTGCGTTTCGCTTTTTGCTTGTAACgg                                                                   >  1:216196/1‑150 (MQ=255)
                                                                                  aGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGt                                                                  <  2:1625884/150‑1 (MQ=255)
                                                                                    aCATTTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCg                                                                <  2:1519294/150‑1 (MQ=255)
                                                                                       tGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAg                                                             >  1:1552457/1‑150 (MQ=255)
                                                                                       tGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAg                                                             <  1:1596128/150‑1 (MQ=255)
                                                                                        ttAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGa                                                            <  1:1690339/149‑1 (MQ=255)
                                                                                        gttaGTATCGACAGTGTGCCGGGGGTCGGCATTTGCCACGTAGAATAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCAGCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGa                                                            <  1:1681323/147‑1 (MQ=255)
                                                                                        gTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGa                                                            >  1:1269524/1‑150 (MQ=255)
                                                                                           aGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCa                                                         >  1:491463/1‑150 (MQ=255)
                                                                                           aGTATAGACAGTGGGCCGGGGGTCGCCATTTGCAACGAAGAACAGCCAACAGTTATGTCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCa                                                         <  1:1308444/150‑1 (MQ=255)
                                                                                            gTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCTACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCAc                                                        >  2:110979/1‑150 (MQ=255)
                                                                                              taaGACAGTGTGACGTGGGTCGCCATTTGCAACGAAGAACAGCCACCCGTAATGGCGGTTGGCTATGGTGTGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCg                                                      <  2:255931/148‑1 (MQ=255)
                                                                                              ataGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCg                                                      <  1:1019435/150‑1 (MQ=255)
                                                                                              ataGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCg                                                      <  1:1025696/150‑1 (MQ=255)
                                                                                               taGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGc                                                     >  1:1324575/1‑150 (MQ=255)
                                                                                                 gACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTg                                                   >  1:1286798/1‑150 (MQ=255)
                                                                                                 gACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTg                                                   >  1:1630086/1‑150 (MQ=255)
                                                                                                     gtgtGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCcag                                               >  2:388708/1‑150 (MQ=255)
                                                                                                         gACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGGGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAgg                                           <  1:1545370/150‑1 (MQ=255)
                                                                                                               ggTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATAACCGCTGCCAGCAGGATTAAg                                     >  2:971540/1‑150 (MQ=255)
                                                                                                                gTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGTTTCGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGc                                    >  1:1559394/1‑150 (MQ=255)
                                                                                                                gTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGc                                    <  2:1652061/150‑1 (MQ=255)
                                                                                                                gTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGAGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGc                                    <  1:258007/150‑1 (MQ=255)
                                                                                                                   gCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCcgc                                 >  1:25271/1‑150 (MQ=255)
                                                                                                                      aTTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAAc                             <  1:1668852/150‑1 (MQ=255)
                                                                                                                        ttGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAAcc                            <  1:1637797/150‑1 (MQ=255)
                                                                                                                         tGCAACGAAGAACAGCCAACAGTTATGGCGGTTGGCTATGTTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACTGTCGAAGATCACCTCTGCCAGCAGGATAAAGCCGCGAAccc                           >  2:1125197/1‑149 (MQ=255)
                                                                                                                          gCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGaacaac                          >  1:515436/1‑150 (MQ=255)
                                                                                                                          gCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGAGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCAc                          >  2:272266/1‑150 (MQ=255)
                                                                                                                           cAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACg                         >  1:1247872/1‑150 (MQ=255)
                                                                                                                           cAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACg                         <  2:1527354/150‑1 (MQ=255)
                                                                                                                            aaCGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACg                         <  2:1662604/150‑1 (MQ=255)
                                                                                                                             aCGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCAACAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCTCGAACCACGTa                       >  1:1255806/1‑150 (MQ=255)
                                                                                                                               gaagaaCAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTGTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCCGGATGAAGCCGCGAACCACGTACt                     >  2:1535751/1‑150 (MQ=255)
                                                                                                                                   aaCAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTgcgc                 <  1:1497270/150‑1 (MQ=255)
                                                                                                                                    ataGCCAACCGCCATAACGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGAAGGATTAAGCCGCGAACCACGTACTGcgcg                >  1:899163/3‑150 (MQ=255)
                                                                                                                                    ataGCCAACCGCCATAACGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCAAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGcgcg                >  2:94506/3‑150 (MQ=255)
                                                                                                                                     cAGCCAACCGTTATGGCTGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGa               <  2:1667993/150‑1 (MQ=255)
                                                                                                                                      aGCCAACCGCCATAACGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGaa              >  2:1377421/1‑150 (MQ=255)
                                                                                                                                       gCCAACCGCCATAACGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGGTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGAGTAAGCAGCGAACCACGTACTGAGCGaaa             >  2:134254/1‑150 (MQ=255)
                                                                                                                                           aCCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGgag         >  1:1400693/1‑150 (MQ=255)
                                                                                                                                                tATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAata    <  1:1712815/150‑1 (MQ=255)
                                                                                                                                                  tGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCAGCGAACAACGTACTGCGCGAAAGGAGAAATAtt  >  1:1495983/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GTTCAGGGGTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCTTTACAGACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATT  >  NC_000913/1982357‑1982652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: