Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,988,050 A→G 5.2% intergenic (‑91/‑172) azuC ← / → yecR acid‑inducible small membrane‑associated protein/lipoprotein, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,988,0500AG 5.2% 355.5 / 6.4 116intergenic (‑91/‑172)azuC/yecRacid‑inducible small membrane‑associated protein/lipoprotein, function unknown
Reads supporting (aligned to +/- strand):  ref base A (54/56);  new base G (4/2);  total (58/58)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 119 total aligned reads displayed.

TCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTGCTTT  >  NC_000913/1987901‑1988199
                                                                                                                                                     |                                                                                                                                                     
tCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACa                                                                                                                                                       >  1:1581220/1‑150 (MQ=255)
tCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTAAAATATGACAACCATCACAAAAATCAATCTTTATGTGATACa                                                                                                                                                       <  2:638357/150‑1 (MQ=255)
          tCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACTTaa                                                                                                                                             >  2:301437/1‑150 (MQ=255)
           cTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATaaa                                                                                                                                            >  2:928912/1‑150 (MQ=255)
             tGCAATAGTTATTGAACATTCTTTTCAGGATTTAGCGCAGTATCATCGCGGCACTCCGACCATTTGTTATTCAGGTGTTATTGTCTTTGCGCTTATAATATGACAAACATCACAAAAATCAATCTATATGTGATACATATCACATAAATa                                                                                                                                          >  2:1542768/1‑150 (MQ=255)
              gCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATTTGTATCACATaaagat                                                                                                                                         <  1:795092/150‑4 (MQ=255)
               cAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATAcc                                                                                                                                        >  2:1474455/1‑150 (MQ=255)
               cAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATAcc                                                                                                                                        >  1:1159078/1‑150 (MQ=255)
               cAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATAcc                                                                                                                                        >  1:512041/1‑150 (MQ=255)
                 aTAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTTTACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCAGATAAATAcccc                                                                                                                                      <  2:937474/150‑1 (MQ=255)
                    gTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCttt                                                                                                                                   <  2:1690347/150‑1 (MQ=255)
                       aTTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTCATACAAATCACATAAATACCCCTTTaaa                                                                                                                                >  2:235780/1‑149 (MQ=255)
                        ttGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATg                                                                                                                               <  2:751076/150‑1 (MQ=255)
                         tGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGt                                                                                                                              <  1:662068/150‑1 (MQ=255)
                         tGAACATACTTTTCAGGATATTGCGCAGTTTCATCGCGTCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCTCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGt                                                                                                                              <  1:229928/150‑1 (MQ=255)
                           aaCATACTTTTCAGGATTTTTCGCATTTTCATCGCGGCACTCCGACCATTTTTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTta                                                                                                                            <  1:495847/150‑1 (MQ=255)
                            aCATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTtat                                                                                                                           <  1:1033935/150‑1 (MQ=255)
                             cATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTtata                                                                                                                          >  1:314587/1‑150 (MQ=255)
                             cATACTTTTCAGGATTTTGCGCAGGTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTtata                                                                                                                          <  2:1524228/150‑1 (MQ=255)
                              aTACTTTTCAGGATTTTGTGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATaa                                                                                                                         <  1:395149/150‑1 (MQ=255)
                              aTACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATaa                                                                                                                         >  1:83488/1‑150 (MQ=255)
                              aTACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTACGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATTTGATACAAATCACATTAATACCCCTTTAATGTTATaa                                                                                                                         >  1:1386153/1‑150 (MQ=255)
                                  ttttCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAAt                                                                                                                     >  2:497874/1‑150 (MQ=255)
                                   tttCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACATGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATg                                                                                                                    <  2:1564426/150‑1 (MQ=255)
                                   tttCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATg                                                                                                                    <  2:1175405/150‑1 (MQ=255)
                                      cAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACCACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATtata                                                                                                                 >  1:1053983/1‑150 (MQ=255)
                                      cAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATa                                                                                                                 <  1:572385/150‑1 (MQ=255)
                                      cAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATa                                                                                                                 >  2:326218/1‑150 (MQ=255)
                                       aGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATaa                                                                                                                <  1:1665458/150‑1 (MQ=255)
                                       aGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATaa                                                                                                                >  1:1179889/1‑150 (MQ=255)
                                       aGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATaa                                                                                                                >  1:1571010/1‑150 (MQ=255)
                                        ggATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAAt                                                                                                               <  1:238244/150‑1 (MQ=255)
                                         gATTTTGAGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATc                                                                                                              <  1:1173090/150‑1 (MQ=255)
                                          aTTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCa                                                                                                             <  2:1198669/150‑1 (MQ=255)
                                             ttGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCaaaa                                                                                                          <  1:1629489/150‑1 (MQ=255)
                                                cgcAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAGTGATAATCAAAAAAc                                                                                                       <  1:718063/150‑1 (MQ=255)
                                                cgcAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAAc                                                                                                       <  1:1442708/150‑1 (MQ=255)
                                                cgcAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATAAAAAAAc                                                                                                       >  1:821243/1‑150 (MQ=255)
                                                 gcAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACa                                                                                                      >  1:1093213/1‑150 (MQ=255)
                                                  cAGTTTCATCGAGGCAATCAGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAg                                                                                                     <  1:228433/150‑1 (MQ=255)
                                                     tttCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGccc                                                                                                  <  2:1505989/150‑1 (MQ=255)
                                                      ttCATCGCGGCACTCCGTCCATTTGTTATTCAGGTGTTATTGTCTTTGAGCTTATATTATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGcccc                                                                                                 <  2:36241/150‑1 (MQ=255)
                                                      ttCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTAAAAAAATGATAATCAAAAAACAGcccc                                                                                                 >  1:1292659/1‑150 (MQ=255)
                                                         aTCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCt                                                                                              >  2:750976/1‑150 (MQ=255)
                                                         aTCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCt                                                                                              >  2:570505/1‑150 (MQ=255)
                                                            gcgGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTAtt                                                                                           >  1:1231024/1‑150 (MQ=255)
                                                            gcgGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTAtt                                                                                           >  2:1080243/1‑150 (MQ=255)
                                                             cgGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTAttt                                                                                          <  2:1266997/150‑1 (MQ=255)
                                                             cgGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTAttt                                                                                          >  1:1185557/1‑150 (MQ=255)
                                                             cgGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTAttt                                                                                          >  1:1192387/1‑150 (MQ=255)
                                                               gCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCt                                                                                        >  1:1732467/1‑150 (MQ=255)
                                                                cACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTg                                                                                       <  2:1707225/150‑1 (MQ=255)
                                                                  atCCGTCGATTTGTTATACATGTGTTATTGTCTTTGCTCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGac                                                                                     <  1:1060312/149‑1 (MQ=255)
                                                                  atCCGATAATTTGTTATACAGGTGTTATTGTCTTTGCGATTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCACTTTAATGTAATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGac                                                                                     <  1:1040441/149‑1 (MQ=255)
                                                                   tCCGACCATTTGTTATACATGTGTTTTTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGaca                                                                                    <  1:684403/150‑1 (MQ=255)
                                                                   tCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAAACATCACAAAAATCAATCTTTATGTGATACAATTCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGaca                                                                                    <  2:86920/150‑1 (MQ=255)
                                                                       aCCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTa                                                                                >  1:494035/1‑150 (MQ=255)
                                                                         cATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACa                                                                              >  2:1504497/1‑150 (MQ=255)
                                                                         cATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCACCCTATTTCTGACACCTACa                                                                              >  2:1563959/1‑150 (MQ=255)
                                                                          aTTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAg                                                                             <  1:1291260/150‑1 (MQ=255)
                                                                            ttGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGAt                                                                           <  2:1079160/150‑1 (MQ=255)
                                                                             tGTTATACAGGGGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAATTCACATAAAGTGCCCTTTAATGTGTTAAAAATGATAATCATAAAACAGCCCACCTATTTATGACACCTACAGATg                                                                          >  2:1169225/1‑150 (MQ=255)
                                                                                tataCAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCa                                                                       >  1:1627069/1‑150 (MQ=255)
                                                                                    cAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGaa                                                                   >  2:783621/1‑150 (MQ=255)
                                                                                    cAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGaa                                                                   <  1:1496268/150‑1 (MQ=255)
                                                                                        tgtTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAg                                                               <  2:209900/150‑1 (MQ=255)
                                                                                         tttATTGTCTTTTCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAgc                                                              <  2:652906/149‑1 (MQ=255)
                                                                                           tATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAgcgc                                                            >  2:222774/1‑150 (MQ=255)
                                                                                            aTTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGcc                                                           >  2:699332/1‑150 (MQ=255)
                                                                                                 cTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCa                                                      <  1:1649741/150‑1 (MQ=255)
                                                                                                   ttGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAgg                                                    <  1:21065/150‑1 (MQ=255)
                                                                                                       gcTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTc                                                <  1:948407/150‑1 (MQ=255)
                                                                                                        cTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCt                                               >  2:892181/1‑150 (MQ=255)
                                                                                                          tataATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCttt                                             >  2:666733/1‑150 (MQ=255)
                                                                                                          tataATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCttt                                             >  2:569100/1‑150 (MQ=255)
                                                                                                           ataataTGACAACCATCACAAAAATCAATCTTTATGTGATACGAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTAtttt                                            >  2:648013/1‑150 (MQ=255)
                                                                                                            taataTGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTc                                           <  2:1571010/150‑1 (MQ=255)
                                                                                                            taataTGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCACCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCAAGGCGTGTTTTc                                           >  2:711532/1‑150 (MQ=255)
                                                                                                                atGACAACCATCACAAAAATCACTCTTTATGTGATACAAATCACATAAATACCCCTTTATTGTTATAAAAATGATAATCAAATAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGc                                       <  2:322072/150‑1 (MQ=255)
                                                                                                                 tGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAACGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGcc                                      >  1:1565361/1‑150 (MQ=255)
                                                                                                                       ccATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTc                                <  2:83488/150‑1 (MQ=255)
                                                                                                                         aTCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCgc                              <  2:379821/150‑1 (MQ=255)
                                                                                                                            acaAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCgcagc                           >  2:446811/1‑150 (MQ=255)
                                                                                                                            acaAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCgcagc                           >  2:555435/1‑150 (MQ=255)
                                                                                                                               aaaaTCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGACCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACt                        >  1:446763/1‑150 (MQ=255)
                                                                                                                                  atcaatcTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTa                     <  2:512041/150‑1 (MQ=255)
                                                                                                                                  atcaatcTTTATGTGATACAAATCACATAAATAACCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTa                     <  2:213567/150‑1 (MQ=255)
                                                                                                                                   tcaatcTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTaa                    >  1:1301039/1‑150 (MQ=255)
                                                                                                                                   tcaatcTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTaa                    <  1:575939/150‑1 (MQ=255)
                                                                                                                                    gggtatttATGTGATTTGTATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAAc                   >  1:991497/6‑150 (MQ=255)
                                                                                                                                    caatcTTAATGTGATACAAATCACATAAATACCCCTGTAATGTTATAAAAATGAAAATCAAAAAACCGCCAACATATTTCTGCCCCCGAGAGATGGCAAGAAATAGCGACTGCCATGCGTCGTTTCCGACCATTGTCGCAGCACTGTAAc                   >  2:668882/1‑150 (MQ=255)
                                                                                                                                      gtatttATGTGATTTGTATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAAcgc                 >  2:1447315/4‑150 (MQ=255)
                                                                                                                                        atttATGTGATACAAATCAAATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGTCGACTTTTCCGGCCATTGTCGCAGCACTGTAACGCGt               >  2:1575242/2‑150 (MQ=255)
                                                                                                                                          ttATGTGATTTGTATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGAAACGCGTaa             >  2:1657350/1‑150 (MQ=255)
                                                                                                                                               tgATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAg        <  1:1474455/150‑1 (MQ=255)
                                                                                                                                               tgATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGAAACGCGTAAAATAg        >  1:1080818/1‑150 (MQ=255)
                                                                                                                                                gATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGt       >  2:1461568/1‑150 (MQ=255)
                                                                                                                                                 aTACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTg      <  1:569100/150‑1 (MQ=255)
                                                                                                                                                  tACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGGCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTACCGCGTAAAATAGTGc     >  2:50767/1‑150 (MQ=255)
                                                                                                                                                     aaaTCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGACCCCCTATTTCTGCCGCCTACAGATGGCAAGAAATAGCTCCTGCCAGGCGTCTTTTCCGGCAATTGTCGCAGAACTGTAAAGCGTAAAATAGTGCttt  >  2:1317194/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTGCTTT  >  NC_000913/1987901‑1988199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: