Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,014,955 T→C 6.6% F26L (TTC→CTC) ‡ fliG → flagellar motor switching and energizing component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,014,9550TC 6.6% 308.1 / 8.4 106F26L (TTC→CTC) ‡fliGflagellar motor switching and energizing component
Reads supporting (aligned to +/- strand):  ref base T (43/56);  new base C (3/4);  total (46/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 111 total aligned reads displayed.

GTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGAT  >  NC_000913/2014808‑2015104
                                                                                                                                                   |                                                                                                                                                     
gTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTc                                                                                                                                                     <  2:404131/150‑1 (MQ=255)
gTATCCGTGAAATGTCTGATAACGATCCGAGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTc                                                                                                                                                     <  2:663148/150‑1 (MQ=255)
    ccGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGc                                                                                                                                                 <  2:1572955/150‑1 (MQ=255)
    ccGTGAAATGTCTGATAACGATCCGCGCGTGGGGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGc                                                                                                                                                 <  1:172795/150‑1 (MQ=255)
      gTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGGCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCAc                                                                                                                                               <  2:923871/150‑1 (MQ=255)
       tGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCAcc                                                                                                                                              <  2:1380238/150‑1 (MQ=255)
       tGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCAcc                                                                                                                                              <  1:418470/150‑1 (MQ=255)
            tGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTcc                                                                                                                                         >  2:422850/1‑150 (MQ=255)
            tGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGCTTGAACACctctgc                                                                                                                                         <  1:1452014/150‑3 (MQ=255)
             gTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTccc                                                                                                                                        <  1:924257/150‑1 (MQ=255)
               cTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGCTTGAACACCTCTgccgc                                                                                                                                      <  1:1256502/150‑3 (MQ=255)
                 gATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCg                                                                                                                                    <  1:1427337/150‑1 (MQ=255)
                   tAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTg                                                                                                                                  <  2:1236152/150‑1 (MQ=255)
                    aaCGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCACAGCGTGa                                                                                                                                 >  2:1285924/1‑150 (MQ=255)
                      cGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGCCCGGGCGGCAGAGGTGTTAAAGCACCTCTCCCAGCGTGAAg                                                                                                                               >  2:1050685/1‑150 (MQ=255)
                      cGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAg                                                                                                                               <  1:1446333/150‑1 (MQ=255)
                      cGATCCGCGCGGGGTGGCGCTGGGCATTCGCCAGTGGATAAATAACGATCATGAGTACCCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAg                                                                                                                               <  2:347928/150‑1 (MQ=255)
                      cGATCAGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCTTGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCATGCACCTCTCCCAGCGTGAAg                                                                                                                               <  2:491985/150‑1 (MQ=255)
                        aTCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTa                                                                                                                             <  1:700303/150‑1 (MQ=255)
                        aTCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGAAGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTa                                                                                                                             <  1:1474643/150‑1 (MQ=255)
                         tCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTAc                                                                                                                            <  2:499273/150‑1 (MQ=255)
                           cgcgcgTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCGGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACaa                                                                                                                          <  1:266487/150‑1 (MQ=255)
                              gcgTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAAcc                                                                                                                       <  2:1542038/150‑1 (MQ=255)
                              gcgTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAAcc                                                                                                                       <  1:1217459/150‑1 (MQ=255)
                                gtggtgGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCt                                                                                                                     <  1:812146/150‑1 (MQ=255)
                                 tgggggCGCTGGTCAATCGCCAGTGGATTAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTg                                                                                                                    <  1:124481/150‑1 (MQ=255)
                                  ggtggCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTgc                                                                                                                   >  2:1201501/1‑149 (MQ=255)
                                       cgcTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCt                                                                                                              >  2:1656306/1‑150 (MQ=255)
                                             tCATTCGCCAGTGGATAAATAACGATCATGAGTAAACTGACACGCACCGATATAAGCGGCATCCTGCTGATGAGCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATg                                                                                                        <  1:1254116/150‑1 (MQ=255)
                                             gcATTCGCCCGGGGCTAACTCACGCTCATGAGTAACCTGCCAGGCACCGATAAAAGCGTCCTCCTGCTGCTGACCATTGGCGAAGACCGGGCGGCAGAGGGGTTCAAGCACCTCTCCCAGCGTGAAGTCCAAACCCTGAGCGCTGCAATg                                                                                                        <  2:135859/149‑1 (MQ=255)
                                              cATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGCGCGCTGCAATgg                                                                                                       >  2:1069388/1‑150 (MQ=255)
                                              cATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATgg                                                                                                       >  2:121761/1‑150 (MQ=255)
                                              cATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATgg                                                                                                       <  2:799106/150‑1 (MQ=255)
                                              cATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATgg                                                                                                       <  1:422850/150‑1 (MQ=255)
                                                 tCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGa                                                                                                    >  2:1602631/1‑150 (MQ=255)
                                                      aGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTc                                                                                               <  1:648239/150‑1 (MQ=255)
                                                       gTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCa                                                                                              <  2:1186303/150‑1 (MQ=255)
                                                        tGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTccc                                                                                             >  2:689632/1‑148 (MQ=255)
                                                         ggATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACg                                                                                            >  1:176597/1‑150 (MQ=255)
                                                          gATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGc                                                                                           >  1:350174/1‑150 (MQ=255)
                                                          gATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTAAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGc                                                                                           >  1:1087538/1‑150 (MQ=255)
                                                           ataaataaCGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCa                                                                                          >  1:1425547/1‑150 (MQ=255)
                                                            taaataaCGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAg                                                                                         >  1:1462211/1‑150 (MQ=255)
                                                             aaataaCGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGa                                                                                        <  1:527267/150‑1 (MQ=255)
                                                                 aaCGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGAtctc                                                                                    <  1:1635942/150‑1 (MQ=255)
                                                                 aaCGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGAtctc                                                                                    >  2:221284/1‑150 (MQ=255)
                                                                  aCGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTcc                                                                                   <  2:70165/150‑1 (MQ=255)
                                                                   cGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCca                                                                                  >  1:1669293/1‑150 (MQ=255)
                                                                   cGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCca                                                                                  <  1:1271408/150‑1 (MQ=255)
                                                                    gATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCcaa                                                                                 >  2:348281/1‑150 (MQ=255)
                                                                     aTCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAACACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGCGCGCTGCAATGGCGAACGTCCCGCAGATCGCcaac                                                                                >  2:1025185/1‑150 (MQ=255)
                                                                      tCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCcaaca                                                                               <  1:44537/150‑1 (MQ=255)
                                                                         tGAGTAACCTGACAGGCACCGATAAAAGCGTAATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTGCCAGCGTGAAGTACAAATCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAagc                                                                            <  2:279353/150‑1 (MQ=255)
                                                                          gAGTAACCTGACAGGCCCCGATAAACGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGTGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAagca                                                                           <  1:62950/150‑1 (MQ=255)
                                                                            gTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAagcagc                                                                         >  2:729939/1‑150 (MQ=255)
                                                                             tacccggACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCt                                                                        <  2:1560739/144‑1 (MQ=255)
                                                                               aCCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTaa                                                                      >  1:72183/1‑150 (MQ=255)
                                                                               aCCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGACCGCTGAAATGGCGAACGTCACGCAGATCTCCAACAAGCATCTaa                                                                      >  1:1148273/1‑150 (MQ=255)
                                                                                     cAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGAtg                                                                <  1:1128259/150‑1 (MQ=255)
                                                                                     cAGGCAACGATAAAATCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCACGCACCTCTCCCAGCGGGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGAtg                                                                <  1:1119011/150‑1 (MQ=255)
                                                                                      aGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGAtgt                                                               <  2:778655/150‑1 (MQ=255)
                                                                                      aGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGAtgt                                                               <  1:700175/150‑1 (MQ=255)
                                                                                         cACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCt                                                            <  1:976972/150‑1 (MQ=255)
                                                                                                aaaaGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACACACCCTGAGCGCGGAAATTGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGCTGTGCTGgaggag                                                     >  2:77055/1‑150 (MQ=255)
                                                                                                 aaaGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGt                                                    >  2:1183607/1‑150 (MQ=255)
                                                                                                 aaaGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCAGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGt                                                    >  2:1049334/1‑150 (MQ=255)
                                                                                                       tCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTAGCGGAGTTTGAGc                                              <  2:1402457/150‑1 (MQ=255)
                                                                                                        cATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAACCCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCa                                             >  2:782634/1‑150 (MQ=255)
                                                                                                        cATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCa                                             >  2:156300/1‑150 (MQ=255)
                                                                                                        cATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCa                                             >  1:1085140/1‑150 (MQ=255)
                                                                                                            ctgctgATGACCATTGGCGAAGCCCGGGCGGCAGAGGTGTTCAAGCCCCTCTCCCAGCGGGAAGTCCAACCCCTGAGCGCTGCACGGGCGAACGTCACGGCGATCTCCCACAAGCCGCTAACCGATGTGCGGGAGGAGTTTGAGCaagac                                         >  1:224903/1‑149 (MQ=255)
                                                                                                            ctgctgATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCaagaa                                         >  1:590775/1‑150 (MQ=255)
                                                                                                             tgctgATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCaagaag                                        >  1:528970/1‑150 (MQ=255)
                                                                                                              gctgATGACCATTGGCGACGACCGGGCGGCAGAGGTGCTCAAGCACCTCTCCCAGCGTGAAGAACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGc                                       <  1:246703/150‑1 (MQ=255)
                                                                                                                tgatgaCCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTg                                     <  1:1575753/150‑1 (MQ=255)
                                                                                                                tgatgaCCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTg                                     <  2:129103/150‑1 (MQ=255)
                                                                                                                  atgaCCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGaa                                   <  2:762015/150‑1 (MQ=255)
                                                                                                                   tgaCCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAAc                                  >  2:1028394/1‑150 (MQ=255)
                                                                                                                      ccATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGt                               >  2:1377092/1‑150 (MQ=255)
                                                                                                                         ttGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTg                            <  1:1656306/150‑1 (MQ=255)
                                                                                                                           ggCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGcc                          <  1:1606026/150‑1 (MQ=255)
                                                                                                                           ggCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGcc                          <  1:159252/150‑1 (MQ=255)
                                                                                                                            gCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGATCGTCACGCAGATCTCCATCAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCg                         >  1:1282833/1‑150 (MQ=255)
                                                                                                                            gCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCg                         <  1:552084/150‑1 (MQ=255)
                                                                                                                             cGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTTCAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGc                        >  1:1072791/1‑150 (MQ=255)
                                                                                                                             cGAAGACAGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACACTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGCTGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGc                        >  1:108416/1‑150 (MQ=255)
                                                                                                                                aGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACt                     >  1:483320/1‑150 (MQ=255)
                                                                                                                                 gACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTg                    >  2:1366801/1‑150 (MQ=255)
                                                                                                                                   ccGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGaa                  <  1:1602631/150‑1 (MQ=255)
                                                                                                                                       ctgggagagGTGCTTGAACACCTCTGCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTg                    <  2:1649673/139‑1 (MQ=255)
                                                                                                                                       ctgggagagGTGCTTGAACACCTCTGCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTg                    >  1:1649673/6‑144 (MQ=255)
                                                                                                                                          gcAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAAc           <  1:1075143/150‑1 (MQ=255)
                                                                                                                                           gagagGTGCTTGAACACCTCTGCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACg          >  2:1149793/2‑150 (MQ=255)
                                                                                                                                               ggTGTTCAAGCACCTCTCCCAGTGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTCCCGCACTGAATATCAACGCCaa      >  2:491482/1‑150 (MQ=255)
                                                                                                                                               ggTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCaa      >  1:1538446/1‑150 (MQ=255)
                                                                                                                                               ggTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCaa      >  1:479299/1‑150 (MQ=255)
                                                                                                                                                 tgtTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTGTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACg    >  2:1033789/1‑150 (MQ=255)
                                                                                                                                                  gtTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGa   >  1:113726/1‑150 (MQ=255)
                                                                                                                                                  gtTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGa   >  1:990591/1‑150 (MQ=255)
                                                                                                                                                   ttCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGAt  <  1:1050685/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GTATCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGAT  >  NC_000913/2014808‑2015104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: