| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,014,958 | A→G | 5.4% | K27E (AAG→GAG) ‡ | fliG → | flagellar motor switching and energizing component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,014,958 | 0 | A | G | 5.4% | 335.0 / 6.9 | 111 | K27E (AAG→GAG) ‡ | fliG | flagellar motor switching and energizing component |
| Reads supporting (aligned to +/- strand): ref base A (48/57); new base G (3/3); total (51/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01 | |||||||||||
Only 100 of 111 total aligned reads displayed.
TCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTA > NC_000913/2014811‑2015106 | ccGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGc < 2:1572955/150‑1 (MQ=255) ccGTGAAATGTCTGATAACGATCCGCGCGTGGGGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGc < 1:172795/150‑1 (MQ=255) gTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGGCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCAc < 2:923871/150‑1 (MQ=255) tGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCAcc < 1:418470/150‑1 (MQ=255) tGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCAcc < 2:1380238/150‑1 (MQ=255) tGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTcc > 2:422850/1‑150 (MQ=255) tGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGCTTGAACACctctgc < 1:1452014/150‑3 (MQ=255) gTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTccc < 1:924257/150‑1 (MQ=255) cTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGCTTGAACACCTCTgccgc < 1:1256502/150‑3 (MQ=255) gATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCg < 1:1427337/150‑1 (MQ=255) tAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTg < 2:1236152/150‑1 (MQ=255) aaCGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCACAGCGTGa > 2:1285924/1‑150 (MQ=255) cGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGCCCGGGCGGCAGAGGTGTTAAAGCACCTCTCCCAGCGTGAAg > 2:1050685/1‑150 (MQ=255) cGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAg < 1:1446333/150‑1 (MQ=255) cGATCCGCGCGGGGTGGCGCTGGGCATTCGCCAGTGGATAAATAACGATCATGAGTACCCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAg < 2:347928/150‑1 (MQ=255) cGATCAGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCTTGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCATGCACCTCTCCCAGCGTGAAg < 2:491985/150‑1 (MQ=255) aTCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTa < 1:700303/150‑1 (MQ=255) aTCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGAAGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTa < 1:1474643/150‑1 (MQ=255) tCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTAc < 2:499273/150‑1 (MQ=255) cgcgcgTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCGGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACaa < 1:266487/150‑1 (MQ=255) gcgTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAAcc < 1:1217459/150‑1 (MQ=255) gcgTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAAcc < 2:1542038/150‑1 (MQ=255) gtggtgGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCt < 1:812146/150‑1 (MQ=255) tgggggCGCTGGTCAATCGCCAGTGGATTAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTg < 1:124481/150‑1 (MQ=255) ggtggCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTgc > 2:1201501/1‑149 (MQ=255) cgcTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCt > 2:1656306/1‑150 (MQ=255) tCATTCGCCAGTGGATAAATAACGATCATGAGTAAACTGACACGCACCGATATAAGCGGCATCCTGCTGATGAGCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATg < 1:1254116/150‑1 (MQ=255) gcATTCGCCCGGGGCTAACTCACGCTCATGAGTAACCTGCCAGGCACCGATAAAAGCGTCCTCCTGCTGCTGACCATTGGCGAAGACCGGGCGGCAGAGGGGTTCAAGCACCTCTCCCAGCGTGAAGTCCAAACCCTGAGCGCTGCAATg < 2:135859/149‑1 (MQ=255) cATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGCGCGCTGCAATgg > 2:1069388/1‑150 (MQ=255) cATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATgg > 2:121761/1‑150 (MQ=255) cATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATgg < 2:799106/150‑1 (MQ=255) cATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATgg < 1:422850/150‑1 (MQ=255) tCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGa > 2:1602631/1‑150 (MQ=255) aGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTc < 1:648239/150‑1 (MQ=255) gTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCa < 2:1186303/150‑1 (MQ=255) ggATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACg > 1:176597/1‑150 (MQ=255) gATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGc > 1:350174/1‑150 (MQ=255) gATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTAAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGc > 1:1087538/1‑150 (MQ=255) ataaataaCGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCa > 1:1425547/1‑150 (MQ=255) taaataaCGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAg > 1:1462211/1‑150 (MQ=255) aaataaCGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGa < 1:527267/150‑1 (MQ=255) aaCGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGAtctc < 1:1635942/150‑1 (MQ=255) aaCGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGAtctc > 2:221284/1‑150 (MQ=255) aCGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTcc < 2:70165/150‑1 (MQ=255) cGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCca > 1:1669293/1‑150 (MQ=255) cGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCca < 1:1271408/150‑1 (MQ=255) gATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCcaa > 2:348281/1‑150 (MQ=255) aTCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAACACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGCGCGCTGCAATGGCGAACGTCCCGCAGATCGCcaac > 2:1025185/1‑150 (MQ=255) tCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCcaaca < 1:44537/150‑1 (MQ=255) tGAGTAACCTGACAGGCACCGATAAAAGCGTAATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTGCCAGCGTGAAGTACAAATCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAagc < 2:279353/150‑1 (MQ=255) gAGTAACCTGACAGGCCCCGATAAACGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGTGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAagca < 1:62950/150‑1 (MQ=255) gTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAagcagc > 2:729939/1‑150 (MQ=255) tacccggACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCt < 2:1560739/144‑1 (MQ=255) aCCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTaa > 1:72183/1‑150 (MQ=255) aCCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGACCGCTGAAATGGCGAACGTCACGCAGATCTCCAACAAGCATCTaa > 1:1148273/1‑150 (MQ=255) cAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGAtg < 1:1128259/150‑1 (MQ=255) cAGGCAACGATAAAATCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCACGCACCTCTCCCAGCGGGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGAtg < 1:1119011/150‑1 (MQ=255) aGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGAtgt < 2:778655/150‑1 (MQ=255) aGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGAtgt < 1:700175/150‑1 (MQ=255) cACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCt < 1:976972/150‑1 (MQ=255) aaaaGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACACACCCTGAGCGCGGAAATTGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGCTGTGCTGgaggag > 2:77055/1‑150 (MQ=255) aaaGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGt > 2:1183607/1‑150 (MQ=255) aaaGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCAGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGt > 2:1049334/1‑150 (MQ=255) tCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTAGCGGAGTTTGAGc < 2:1402457/150‑1 (MQ=255) cATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAACCCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCa > 2:782634/1‑150 (MQ=255) cATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCa > 1:1085140/1‑150 (MQ=255) cATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCa > 2:156300/1‑150 (MQ=255) ctgctgATGACCATTGGCGAAGCCCGGGCGGCAGAGGTGTTCAAGCCCCTCTCCCAGCGGGAAGTCCAACCCCTGAGCGCTGCACGGGCGAACGTCACGGCGATCTCCCACAAGCCGCTAACCGATGTGCGGGAGGAGTTTGAGCaagac > 1:224903/1‑149 (MQ=255) ctgctgATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCaagaa > 1:590775/1‑150 (MQ=255) tgctgATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCaagaag > 1:528970/1‑150 (MQ=255) gctgATGACCATTGGCGACGACCGGGCGGCAGAGGTGCTCAAGCACCTCTCCCAGCGTGAAGAACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGc < 1:246703/150‑1 (MQ=255) tgatgaCCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTg < 2:129103/150‑1 (MQ=255) tgatgaCCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTg < 1:1575753/150‑1 (MQ=255) atgaCCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGaa < 2:762015/150‑1 (MQ=255) tgaCCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAAc > 2:1028394/1‑150 (MQ=255) ccATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGt > 2:1377092/1‑150 (MQ=255) ttGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTg < 1:1656306/150‑1 (MQ=255) ggCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGcc < 1:1606026/150‑1 (MQ=255) ggCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGcc < 1:159252/150‑1 (MQ=255) gCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGATCGTCACGCAGATCTCCATCAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCg > 1:1282833/1‑150 (MQ=255) gCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAGCGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCg > 2:675514/1‑150 (MQ=255) gCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCg < 1:552084/150‑1 (MQ=255) cGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTTCAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGc > 1:1072791/1‑150 (MQ=255) cGAAGACAGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACACTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGCTGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGc > 1:108416/1‑150 (MQ=255) aGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACt > 1:483320/1‑150 (MQ=255) gACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTg > 2:1366801/1‑150 (MQ=255) ccGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGaa < 1:1602631/150‑1 (MQ=255) ctgggagagGTGCTTGAACACCTCTGCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTg > 1:1649673/6‑144 (MQ=255) ctgggagagGTGCTTGAACACCTCTGCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTg < 2:1649673/139‑1 (MQ=255) gcAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAAc < 1:1075143/150‑1 (MQ=255) gagagGTGCTTGAACACCTCTGCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACg > 2:1149793/2‑150 (MQ=255) ggTGTTCAAGCACCTCTCCCAGTGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTCCCGCACTGAATATCAACGCCaa > 2:491482/1‑150 (MQ=255) ggTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCaa > 1:479299/1‑150 (MQ=255) ggTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCaa > 1:1538446/1‑150 (MQ=255) tgtTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTGTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACg > 2:1033789/1‑150 (MQ=255) gtTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGa > 1:113726/1‑150 (MQ=255) gtTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGa > 1:990591/1‑150 (MQ=255) ttCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGAt < 1:1050685/150‑1 (MQ=255) cAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTa > 2:574501/1‑150 (MQ=255) cAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTa > 2:699769/1‑150 (MQ=255) | TCCGTGAAATGTCTGATAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAACCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTA > NC_000913/2014811‑2015106 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |