Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,015,698 A→T 7.3% K273N (AAA→AAT fliG → flagellar motor switching and energizing component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,015,6980AT 7.3% 298.2 / 7.6 102K273N (AAA→AATfliGflagellar motor switching and energizing component
Reads supporting (aligned to +/- strand):  ref base A (53/41);  new base T (2/6);  total (55/47)
Fisher's exact test for biased strand distribution p-value = 1.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

AGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAACTGGCGAGATGGTAAT  >  NC_000913/2015550‑2015847
                                                                                                                                                    |                                                                                                                                                     
    ggCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCtt                                                                                                                                                  >  1:188271/1‑150 (MQ=255)
      cgcAGAAAATCCTCGACGAGAGGTTCCTGTTCGAGAAGCTGGTGGATGTCGACGATCGCAGCATTCAGCGGCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTgc                                                                                                                                                <  1:1163882/150‑1 (MQ=255)
      cgcAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTgc                                                                                                                                                <  1:1489495/150‑1 (MQ=255)
      cgcAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCAAGAATTTCTCgc                                                                                                                                                <  1:219538/150‑1 (MQ=255)
       gcAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTgcg                                                                                                                                               <  1:186975/150‑1 (MQ=255)
       gcAGAAAAGCATCGACGAGATGTTCCTGTTCCAGAAGCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTgcg                                                                                                                                               <  2:1256645/150‑1 (MQ=255)
        cAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCAAGAATTTCTCgcgc                                                                                                                                              <  1:1718997/150‑1 (MQ=255)
         aGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCa                                                                                                                                             >  1:408708/1‑150 (MQ=255)
          gAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCAAGAATTTCTCGCGCag                                                                                                                                            <  1:1160737/150‑2 (MQ=255)
           aaaaTCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAat                                                                                                                                           <  2:734145/150‑1 (MQ=255)
           aaaaTCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCAAGAATTTCTCGCGCagt                                                                                                                                           <  1:15962/150‑3 (MQ=255)
           aaaaTCAACGACGAGCTGTTCCTGAGCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAat                                                                                                                                           <  2:727784/150‑1 (MQ=255)
            aaaTCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGCGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAata                                                                                                                                          >  1:44470/1‑150 (MQ=255)
             aaTCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAatat                                                                                                                                         >  1:467435/1‑150 (MQ=255)
             aaTCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAatat                                                                                                                                         <  1:998255/150‑1 (MQ=255)
              atcatcGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCAAGAATTTCTCGCGCagtggc                                                                                                                                        <  1:697413/150‑6 (MQ=255)
               tcatcGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCAAGAATTTCTCGCGCagtggct                                                                                                                                       <  1:492523/150‑7 (MQ=255)
                 atcGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCg                                                                                                                                     >  2:1532239/1‑150 (MQ=255)
                 atcGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCg                                                                                                                                     >  1:490030/1‑150 (MQ=255)
                   cgacgaGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCa                                                                                                                                   >  2:1001069/1‑150 (MQ=255)
                   cgacgaGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCa                                                                                                                                   >  1:374217/1‑150 (MQ=255)
                    gacgaGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAg                                                                                                                                  <  2:771863/150‑1 (MQ=255)
                     acgaGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGc                                                                                                                                 >  2:1625225/1‑150 (MQ=255)
                         gaTGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCTAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTCCGCGAGAAATTCTTGCGCAATATTTCGCAGCGTgc                                                                                                                             >  1:321019/1‑150 (MQ=255)
                                cTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAACGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGat                                                                                                                      <  2:64181/150‑1 (MQ=255)
                                cTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGat                                                                                                                      >  1:640509/1‑150 (MQ=255)
                                cTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGat                                                                                                                      >  1:594588/1‑150 (MQ=255)
                                cTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGat                                                                                                                      <  1:918444/150‑1 (MQ=255)
                                  gTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCCGCGTGCCGCCGatat                                                                                                                    >  2:908180/1‑150 (MQ=255)
                                  gTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGatat                                                                                                                    >  1:668085/1‑150 (MQ=255)
                                     cGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGTAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCt                                                                                                                 >  2:369973/1‑150 (MQ=255)
                                     cGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCt                                                                                                                 >  2:1608141/1‑150 (MQ=255)
                                     cGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCt                                                                                                                 >  2:611877/1‑150 (MQ=255)
                                      gagaATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTg                                                                                                                >  1:322536/1‑150 (MQ=255)
                                      gagaATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTg                                                                                                                >  1:409977/1‑150 (MQ=255)
                                       agaATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTgc                                                                                                               <  2:984252/150‑1 (MQ=255)
                                                    tGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGc                                                                                                  <  2:188271/150‑1 (MQ=255)
                                                    tGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGc                                                                                                  <  2:1027954/150‑1 (MQ=255)
                                                     gTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGcc                                                                                                 >  2:28636/1‑150 (MQ=255)
                                                      tCGCCGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCAACTGCGCGAGTAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCa                                                                                                >  2:1459846/1‑150 (MQ=255)
                                                       cgacgaTCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCaa                                                                                               >  2:1474597/1‑150 (MQ=255)
                                                       cgacgaTCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCaa                                                                                               >  1:645537/1‑150 (MQ=255)
                                                          cgaTCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAATTTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCg                                                                                            >  1:669852/1‑150 (MQ=255)
                                                           gaTCGCAGCATTCAGCGTCTGTTGCAGGAAGGGGATGCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCCGCGCGACGATCTCGCCAACCGt                                                                                           <  1:483159/150‑1 (MQ=255)
                                                            aTCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTg                                                                                          >  1:1592407/1‑150 (MQ=255)
                                                             tCGCAGCATTCAGCGTCTGTTGCAGCAAGTGGATTCCGAATCGCTGTTGAGCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTgg                                                                                         <  1:1288169/150‑1 (MQ=255)
                                                               gcagcaTTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTc                                                                                       >  1:653833/1‑150 (MQ=255)
                                                                cagcaTTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTcc                                                                                      <  1:9710/150‑1 (MQ=255)
                                                                  gcaTTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCgg                                                                                    <  1:215648/150‑1 (MQ=255)
                                                                    aTTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTg                                                                                  <  1:611877/150‑1 (MQ=255)
                                                                     ttCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGc                                                                                 <  1:946592/150‑1 (MQ=255)
                                                                     ttCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGc                                                                                 >  2:626382/1‑150 (MQ=255)
                                                                     ttCAGCGTCGGTTGCAGGAAGGGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGc                                                                                 <  2:101133/150‑1 (MQ=255)
                                                                        aGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTc                                                                              <  2:392888/150‑1 (MQ=255)
                                                                           gTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGt                                                                           >  2:1381777/1‑150 (MQ=255)
                                                                               gTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCa                                                                       <  2:467435/150‑1 (MQ=255)
                                                                                ttGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAg                                                                      <  2:640509/150‑1 (MQ=255)
                                                                                ttGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAg                                                                      <  2:594588/150‑1 (MQ=255)
                                                                                  gCAGGAAGTGGATTCCGACTCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGt                                                                    <  1:526546/150‑1 (MQ=255)
                                                                                  gCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGt                                                                    >  2:583868/1‑150 (MQ=255)
                                                                                   cAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTg                                                                   <  1:1625225/150‑1 (MQ=255)
                                                                                    aGGAAGTGGATTCCGACTCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTgg                                                                  <  2:1506490/150‑1 (MQ=255)
                                                                                    aGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTgg                                                                  >  2:297478/1‑150 (MQ=255)
                                                                                      gAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCTGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGaa                                                                >  1:314521/1‑150 (MQ=255)
                                                                                       aaGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCACCCGTGGTCCGGGGCGTCTGTCGCAGGTGGaaa                                                               >  2:101271/1‑150 (MQ=255)
                                                                                         gTGGATTCCGAATCGCTGTTGATCGCTCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAAc                                                             <  1:1334521/150‑1 (MQ=255)
                                                                                         gTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAAc                                                             <  2:506455/150‑1 (MQ=255)
                                                                                            gATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCAGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGCAAAACGaa                                                          >  2:261052/1‑150 (MQ=255)
                                                                                             aTTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAAc                                                         <  2:767912/150‑1 (MQ=255)
                                                                                                        cTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGAtt                                              >  1:1079043/1‑150 (MQ=255)
                                                                                                         tGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAACACGAACAGAAAGCGATTc                                             >  1:21082/1‑150 (MQ=255)
                                                                                                         tGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTc                                             <  2:1467880/150‑1 (MQ=255)
                                                                                                         tGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTc                                             <  2:137614/150‑1 (MQ=255)
                                                                                                           ttGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTctg                                           <  1:78046/150‑1 (MQ=255)
                                                                                                            tGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTctgc                                          <  2:1684824/150‑1 (MQ=255)
                                                                                                             gATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTctgct                                         <  1:1608141/150‑1 (MQ=255)
                                                                                                             gATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTctgct                                         >  1:658423/1‑150 (MQ=255)
                                                                                                              aTCGCGCTGACAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGGGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTctgctg                                        <  1:1655208/150‑1 (MQ=255)
                                                                                                               tCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGa                                       <  1:1286348/150‑1 (MQ=255)
                                                                                                                 gcgcTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGAtt                                     >  1:821405/1‑150 (MQ=255)
                                                                                                                  cgcTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAACGCGATTCTGCTGATtg                                    >  2:1660238/1‑150 (MQ=255)
                                                                                                                     tGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTgc                                 >  1:505407/1‑150 (MQ=255)
                                                                                                                      gAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTgcg                                >  2:14335/1‑150 (MQ=255)
                                                                                                                      gAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTgcg                                >  2:1290041/1‑150 (MQ=255)
                                                                                                                       aaaGGAGCCGAGCATCCACTGCGCGAGAAATTATTGCTCAATATGTCGCAGCGTGCCGCCGATATTCTGAGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAACACGACCAGAAAGCGATTCTGCTGATTGTgcgc                               >  2:1450226/1‑150 (MQ=255)
                                                                                                                         aGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGcgccg                             >  2:516083/1‑150 (MQ=255)
                                                                                                                         aGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGcgccg                             <  1:207737/150‑1 (MQ=255)
                                                                                                                            aGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCt                          >  2:62005/1‑150 (MQ=255)
                                                                                                                             gCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCtt                         >  2:818803/1‑150 (MQ=255)
                                                                                                                               cGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCCGCTGATTGTGCGCCGCCTTGc                       >  1:604226/1‑150 (MQ=255)
                                                                                                                                  gcagcCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATATCGCCAACCGTGGTCAGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGa                    >  2:1091746/1‑150 (MQ=255)
                                                                                                                                   cagcCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGAGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGaa                   >  2:697374/1‑150 (MQ=255)
                                                                                                                                    agcCACTGCGCGAGAAATTCTTGCGCAATATGTCGCCGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTCGATAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGaaa                  >  2:380468/1‑150 (MQ=255)
                                                                                                                                     gcCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAAc                 >  2:507648/1‑150 (MQ=255)
                                                                                                                                       tattGCGCAAGAATTTCTCGCGCAGTATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAACTg               >  1:391260/4‑150 (MQ=255)
                                                                                                                                        aCTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAACTgg              <  1:347836/150‑1 (MQ=255)
                                                                                                                                            cgcgAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAACTGGCgag          >  2:1415253/1‑150 (MQ=255)
                                                                                                                                               gagaAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAACTGGCGAGATg       <  2:408708/150‑1 (MQ=255)
                                                                                                                                                agaAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAACTGGCGAGATgg      <  1:1081276/150‑1 (MQ=255)
                                                                                                                                                    aTTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAACTGGCGAGATGGTAAt  <  1:682267/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGCTGATTGTGCGCCGCCTTGCCGAAACTGGCGAGATGGTAAT  >  NC_000913/2015550‑2015847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: