Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,019,460 G→A 100% V359I (GTC→ATC)  fliK → flagellar hook‑length control protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,019,4600GA100.0% 169.1 / NA 55V359I (GTC→ATC) fliKflagellar hook‑length control protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/32);  total (23/32)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGA  >  NC_000913/2019321‑2019605
                                                                                                                                           |                                                                                                                                                 
cATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACCGCAAACCATAAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACa                                                                                                                                         >  2:1529411/1‑150 (MQ=255)
cATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACa                                                                                                                                         >  2:1731094/1‑150 (MQ=255)
    cAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAggg                                                                                                                                     <  1:512117/150‑1 (MQ=255)
         aGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCCCAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCgtgt                                                                                                                                >  2:182805/1‑150 (MQ=255)
          gggCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACTATACGCTTCCGGTTCCCATCACTTTACAAGGGCGTGTa                                                                                                                               >  2:77375/1‑150 (MQ=255)
          gggCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTa                                                                                                                               <  1:1388663/150‑1 (MQ=255)
           ggCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTaa                                                                                                                              >  1:1720490/1‑150 (MQ=255)
            gCAAAGCAACATCAGTGGCGAAAGCTTTAGTGTTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCAAACGCCCAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGTTTCCCATCTAGTTACAAGGGCGTGTAAc                                                                                                                             >  2:1724170/1‑150 (MQ=255)
             cAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGCCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACa                                                                                                                            <  1:225460/150‑1 (MQ=255)
               aaGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCATCAGGCCGGTGCCCAGCTACTGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACTACGATACGCTTCCGTTTCCCATCTCTTTACAAGGGCGTGTAACAgg                                                                                                                          <  2:1349216/150‑1 (MQ=255)
                 gCAACATCAGTTGCGAAATCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAAAAGCAAAGCCAACTCACAGCAAACCATGAAACTCTGGCGGGGGAAGACGACTATACGCTTCCGTTTCCCATCTCTTTACAAGGGCGTGTAACAGGCa                                                                                                                        <  1:1616846/150‑1 (MQ=255)
                     cATCAGTGGATAAAGCTTTAGTGGTCAGCAGCACGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCAAGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCACATCTCTAAACAAGGACGTGTAAAAGGCAACAg                                                                                                                    >  1:564314/1‑150 (MQ=255)
                                     tttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTc                                                                                                    <  1:182805/150‑1 (MQ=255)
                                     tttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTc                                                                                                    <  1:975376/150‑1 (MQ=255)
                                        aGTGGTCAGCAGCAGGCCGCTTCCCAGCAACTGCAAAGCCAACCAACAGCAAACCATGAACCTCTGGCGGGGGATGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGcc                                                                                                 <  1:1471980/150‑1 (MQ=255)
                                           ggTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGCCGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTaa                                                                                              >  2:1026336/1‑150 (MQ=255)
                                           ggTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTaa                                                                                              >  1:1620660/1‑150 (MQ=255)
                                            gTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAAc                                                                                             <  1:601610/150‑1 (MQ=255)
                                                gcagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGCGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCa                                                                                         >  2:462396/1‑150 (MQ=255)
                                                  agcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAACCGCGTGTAACAGGCAACAGCCGCGTTGATATTTTCGCCTATCGTCaga                                                                                       >  2:169935/1‑150 (MQ=255)
                                                   gcaggcagCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATTAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTACCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCagag                                                                                      <  2:216896/150‑1 (MQ=255)
                                                    cagGCCGCTACCCAGCAACAGCAAAGCCAACGCACACCAAACCATGAACCTCTGCCGGGGGAAGACGACGATACGCTTCCGCTTCCCATCTCATTACAAGGGCGAGATACAGGCAACAGCGGCGTTCATAATTTCGCCTATCGTCAGCgg                                                                                     >  1:1323442/1‑150 (MQ=255)
                                                        ccGCTTCCCAGCAACAGCCAAGCCAACGCACAGCAACCCATGCACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCGCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGc                                                                                 <  2:150747/150‑1 (MQ=255)
                                                          gCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCAc                                                                               <  2:1720490/150‑1 (MQ=255)
                                                           cTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCAcc                                                                              <  2:651245/150‑1 (MQ=255)
                                                            ttCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCg                                                                             <  2:1241644/150‑1 (MQ=255)
                                                            ttCCCAGCAACAGCAAACCCAACGCACAGCAAACTATTAACCTCTGGCGGGGGAAGACGAGGTTACTCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGTCAACAGTGTCTTTGATATTTTCGCCTAACGTCAGAGGTAGCACCg                                                                             <  1:847460/150‑1 (MQ=255)
                                                             tCCCAGCACCAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGTAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGt                                                                            <  1:624255/150‑1 (MQ=255)
                                                               ccAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATATTCGCCTAACGTCAGAGGTAGCACCGTaa                                                                          >  1:908553/1‑150 (MQ=255)
                                                                cagcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAAt                                                                         >  2:1028278/1‑150 (MQ=255)
                                                                  gcaacctcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTTCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGGCAGAGGTAGCACCGTAATcc                                                                       <  2:1536740/150‑1 (MQ=255)
                                                                      cagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGt                                                                   >  1:469948/1‑150 (MQ=255)
                                                                       agcacAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTc                                                                  <  2:213882/150‑1 (MQ=255)
                                                                       agcaaAGCCAACGCACAGCACACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTc                                                                  <  2:219321/150‑1 (MQ=255)
                                                                       agcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTc                                                                  >  1:1160927/1‑150 (MQ=255)
                                                                            aGCCAACTCACAGCAAACCTTGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTc                                                             <  1:413219/150‑1 (MQ=255)
                                                                            aGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTc                                                             >  2:942587/1‑150 (MQ=255)
                                                                             gCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTcc                                                            <  1:890685/150‑1 (MQ=255)
                                                                              ccAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTccc                                                           >  2:486356/1‑150 (MQ=255)
                                                                              ccAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTccc                                                           >  1:444423/1‑150 (MQ=255)
                                                                                  cGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCt                                                       <  2:1264811/150‑1 (MQ=255)
                                                                                  cGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGGTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCt                                                       <  1:1523774/150‑1 (MQ=255)
                                                                                   gCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTAAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCtt                                                      >  2:1013459/1‑150 (MQ=255)
                                                                                    cacaGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATATGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCttt                                                     <  2:774478/150‑1 (MQ=255)
                                                                                        gCAAACCATGTACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTttgttg                                                 <  1:1091703/150‑1 (MQ=255)
                                                                                        gCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTttgttg                                                 <  2:1684216/150‑1 (MQ=255)
                                                                                        gCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTttgttg                                                 <  1:1028278/150‑1 (MQ=255)
                                                                                             ccATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTc                                            >  1:469705/1‑150 (MQ=255)
                                                                                                  aaCCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGAc                                       >  2:736546/1‑150 (MQ=255)
                                                                                                     ctctGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACTAGGGCGTGTAACAGGGAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCa                                    <  2:734239/150‑1 (MQ=255)
                                                                                                           cGGGGGAAGACGACGATAAGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATaa                              >  2:88309/1‑150 (MQ=255)
                                                                                                                            aCGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTa             <  1:789521/150‑1 (MQ=255)
                                                                                                                                 tCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGac        <  1:1731094/150‑1 (MQ=255)
                                                                                                                                       tCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGa  <  2:29102/150‑1 (MQ=255)
                                                                                                                                       tCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGa  <  1:964612/150‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                 
CATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGA  >  NC_000913/2019321‑2019605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑