Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,024,609 T→G 5.6% intergenic (+18/+26) rcsA → / ← dsrB transcriptional regulator of colanic acid capsular biosynthesis/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,024,6090TG 5.6% 352.9 / 9.8 125intergenic (+18/+26)rcsA/dsrBtranscriptional regulator of colanic acid capsular biosynthesis/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (60/58);  new base G (4/3);  total (64/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.26e-01

Only 100 of 129 total aligned reads displayed.

AAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTG  >  NC_000913/2024460‑2024756
                                                                                                                                                     |                                                                                                                                                   
 aaGCCAAGACCGTTTCATCGCATTAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGGCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGtt                                                                                                                                                    <  1:129291/150‑1 (MQ=255)
   gcaaaGACCGTTTCATCGCATATAGGTAATATTTAACGTAAGATCAAAACGCATAATAAACAGGTTATCTTCCATGTCGTCCGTCTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTc                                                                                                                                                  <  1:1041690/147‑1 (MQ=255)
     cAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTccc                                                                                                                                                <  2:1345474/150‑1 (MQ=255)
      aaGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCcca                                                                                                                                               <  2:1712534/150‑1 (MQ=255)
        gACCGGTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGGGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCccacc                                                                                                                                             <  2:470749/150‑1 (MQ=255)
           cGTATCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGt                                                                                                                                          <  2:429254/150‑1 (MQ=255)
            gTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTc                                                                                                                                         <  1:268171/150‑1 (MQ=255)
            gTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGGAAACCACCAGTc                                                                                                                                         <  1:1484433/150‑1 (MQ=255)
               tCATCGCATAAAGGTAATATTAAACGTAAGAGCAAATCGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGTCTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAgg                                                                                                                                      <  2:1144226/150‑1 (MQ=255)
                 aTCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCt                                                                                                                                    <  2:349927/150‑1 (MQ=255)
                 aTCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCt                                                                                                                                    >  2:475713/1‑150 (MQ=255)
                  tCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTg                                                                                                                                   <  2:1011832/150‑1 (MQ=255)
                  tCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGGAAACCACCAGTCAgaatg                                                                                                                                   <  2:1085770/150‑5 (MQ=255)
                  acGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGATATCTACCATGGCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTg                                                                                                                                   <  2:463110/149‑1 (MQ=255)
                   cGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACAAGCGCTAACACATTCTAACTGGTGGTTTCCCACCAGTCAGGCTGa                                                                                                                                  >  2:1299916/1‑150 (MQ=255)
                    gCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGaa                                                                                                                                 >  1:719558/1‑150 (MQ=255)
                     cATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAAt                                                                                                                                <  2:292780/150‑1 (MQ=255)
                       tAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATaa                                                                                                                              <  2:1334910/150‑1 (MQ=255)
                        aaaGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAg                                                                                                                             >  1:1452666/1‑150 (MQ=255)
                          aGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTAAAATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTAAACATGCGATAACACATTATGACTGGTGGTTTCCGACAAGTCAGGCTGATTAAAAt                                                                                                                           >  1:32580/1‑150 (MQ=255)
                           ggTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGAtt                                                                                                                          >  1:650409/1‑150 (MQ=255)
                           ggTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGAtt                                                                                                                          >  2:1505863/1‑150 (MQ=255)
                             tAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTAc                                                                                                                        <  1:963684/150‑1 (MQ=255)
                               atatTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTActc                                                                                                                      <  2:278870/150‑1 (MQ=255)
                                 atTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTg                                                                                                                    >  2:898158/1‑150 (MQ=255)
                                 atTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTAACATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTg                                                                                                                    >  2:1000782/1‑150 (MQ=255)
                                   tAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTAGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCt                                                                                                                  <  1:200161/150‑1 (MQ=255)
                                    aaaCGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCtt                                                                                                                 >  1:1388196/1‑150 (MQ=255)
                                           aGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCcac                                                                                                          <  2:832346/150‑1 (MQ=255)
                                           aGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCAACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCcac                                                                                                          >  1:1021820/1‑150 (MQ=255)
                                            gATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCcaca                                                                                                         >  1:350424/1‑150 (MQ=255)
                                                aaaaCGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGa                                                                                                     <  1:1674660/150‑1 (MQ=255)
                                                  aaCGCATAATAAACTGGTTATGTACCATGTGGTCCGAGTCACGTATAATGTGACTAATGGTATTTTTGTCAAAATGCGCTAACCCATTCTGACTGGTGGTTTCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTATCCACAAAGATAc                                                                                                  <  1:295850/150‑1 (MQ=255)
                                                   aCGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATAc                                                                                                  >  1:434727/1‑150 (MQ=255)
                                                     gCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCg                                                                                                <  2:719558/150‑1 (MQ=255)
                                                         aataaACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGCTTACTCTTCTTTCTCCACAAAGATACCGTCCt                                                                                            >  2:1142540/1‑150 (MQ=255)
                                                         aataaACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCt                                                                                            >  2:467747/1‑150 (MQ=255)
                                                            aaaCAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGCTTACTCTGCTTTCTCAACAAAGATACCGTCCTGAt                                                                                         >  2:742903/1‑150 (MQ=255)
                                                            aaaCAGGTTATCTACCATGTCGTCCCACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCCCCAGTCAGTATGAATAAGATTACTATGCTTACTCCACAAATCTACACTCCTGAt                                                                                         >  2:605258/1‑150 (MQ=255)
                                                             aaCAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATg                                                                                        <  2:1067936/150‑1 (MQ=255)
                                                                aGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGccc                                                                                     <  2:857904/150‑1 (MQ=255)
                                                                   ttATCTACCATGTCGTCCGACTGACTGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGTTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGc                                                                                  <  2:534448/150‑1 (MQ=255)
                                                                    tATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCt                                                                                 <  1:1310380/150‑1 (MQ=255)
                                                                     aTCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCtt                                                                                <  2:1533220/150‑1 (MQ=255)
                                                                      tCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCCGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTc                                                                               >  1:455438/1‑150 (MQ=255)
                                                                        tACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCAt                                                                             >  2:16625/1‑150 (MQ=255)
                                                                         aCCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACGTTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCAtt                                                                            >  2:164295/1‑150 (MQ=255)
                                                                         aCCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCAtt                                                                            <  2:1668554/150‑1 (MQ=255)
                                                                         aCCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCAtt                                                                            <  2:1720709/150‑1 (MQ=255)
                                                                           cATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTaa                                                                          <  2:924207/150‑1 (MQ=255)
                                                                              gtcgtcCGACTGACGGATAATGTGACTAATGGTAATTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCCTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGa                                                                       <  1:1148012/150‑1 (MQ=255)
                                                                               tcgtcCGACTGACGGAAAATGTGACTATTCGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGAGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTCCTGTGTCATCAAAGATACCGTCCTGATGCCCTGATGAATTAAAGaa                                                                      >  1:273452/1‑150 (MQ=255)
                                                                                cgtcCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAAc                                                                     >  2:1212189/1‑150 (MQ=255)
                                                                                cgtcCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAAc                                                                     >  2:730710/1‑150 (MQ=255)
                                                                                 gtcCGACTGACTGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAAcc                                                                    <  2:495096/150‑1 (MQ=255)
                                                                                 gtcCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAAcc                                                                    <  2:577089/150‑1 (MQ=255)
                                                                                 gtcCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTATGCTTTCTCCAAAAAGATACCGTCCTGATGCACTGCTTCATTAAAGAAca                                                                    >  1:1074518/1‑149 (MQ=255)
                                                                                   ccGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAg                                                                  <  2:735289/150‑1 (MQ=255)
                                                                                       cTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGc                                                              <  1:475336/150‑1 (MQ=255)
                                                                                       cTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGGCCTGATGCCCTGCTTCATTAAAGAACCAGATGc                                                              >  1:1395666/1‑150 (MQ=255)
                                                                                         gACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCg                                                            >  1:232460/1‑150 (MQ=255)
                                                                                         gACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCg                                                            <  1:437711/150‑1 (MQ=255)
                                                                                             gATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCCCCAGTCAGGCTGAATGAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCg                                                        >  2:824385/1‑150 (MQ=255)
                                                                                             gATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCg                                                        <  1:1245836/150‑1 (MQ=255)
                                                                                              aTAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCgg                                                       <  1:630663/150‑1 (MQ=255)
                                                                                               tAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCggg                                                      <  1:1600071/150‑1 (MQ=255)
                                                                                                aaTGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGt                                                     >  1:492109/1‑150 (MQ=255)
                                                                                                  tgtgACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAg                                                   <  1:1561645/150‑1 (MQ=255)
                                                                                                  tgtgACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGGGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATTCCGAGCGGGTAg                                                   <  1:827883/150‑1 (MQ=255)
                                                                                                    tgACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTc                                                 >  1:1390063/1‑150 (MQ=255)
                                                                                                     taCTAATGGTATTTTTGTCAAAATGCGCTAACACATTCTGACTTGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCt                                                <  1:1078010/149‑1 (MQ=255)
                                                                                                      aCTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCtt                                               <  1:1525741/150‑1 (MQ=255)
                                                                                                       cTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTc                                              >  1:1309267/1‑150 (MQ=255)
                                                                                                         aaTGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCa                                            >  2:829581/1‑150 (MQ=255)
                                                                                                           tGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGc                                          <  2:1381676/150‑1 (MQ=255)
                                                                                                               aTTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGaaa                                      >  2:1488042/1‑150 (MQ=255)
                                                                                                                tttttGTCAACATGCGCTAACACATTCTGACTGGTGGTGTCCCACCAGGCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTGCATTAAAGAACCAGATGCCGAGCGGGGAGTCTTCCAGCGAAAc                                     >  2:1713954/1‑150 (MQ=255)
                                                                                                                  tttGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCa                                   <  2:498648/150‑1 (MQ=255)
                                                                                                                    tGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAgg                                 <  2:180782/150‑1 (MQ=255)
                                                                                                                     gTCAACATGAGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCATTCTCCACAAAGATACCGTCCTGCTGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAACCCAGGt                                >  2:1589873/1‑150 (MQ=255)
                                                                                                                       cAACATGCGCTAACACATTCTGACTGTTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTAc                              <  1:753721/150‑1 (MQ=255)
                                                                                                                       cAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTAc                              >  2:1145299/1‑150 (MQ=255)
                                                                                                                       cAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTAc                              >  2:1011492/1‑150 (MQ=255)
                                                                                                                        aaCATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACa                             >  2:503706/1‑150 (MQ=255)
                                                                                                                           aTGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATtg                          <  1:1532331/150‑1 (MQ=255)
                                                                                                                           aTGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATtg                          <  2:143589/150‑1 (MQ=255)
                                                                                                                              cgcTAACACATTCTGACTGGTGGGTTCCCACCAGTCAGGCGGAATTAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGGTTCATTAAAGAACCAGCTGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGc                       <  2:273452/150‑1 (MQ=255)
                                                                                                                                cTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCt                     <  2:434727/150‑1 (MQ=255)
                                                                                                                                  aaCACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGATACCAGGTACATTGTGCCTTc                   >  1:774817/1‑150 (MQ=255)
                                                                                                                                  aaCACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTc                   >  2:1662743/1‑150 (MQ=255)
                                                                                                                                   acacATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTGCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCa                  <  1:980219/150‑1 (MQ=255)
                                                                                                                                        gccTGACTGGTGGGAAACCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTaca             >  1:57378/3‑148 (MQ=255)
                                                                                                                                         tCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAAc            >  2:1602258/1‑150 (MQ=255)
                                                                                                                                         ccTGACTGGTGGGAAACCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAAc            >  1:669770/2‑150 (MQ=255)
                                                                                                                                          cTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAACct           >  2:1548778/1‑150 (MQ=255)
                                                                                                                                          cTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAAct           >  1:1277305/1‑150 (MQ=255)
                                                                                                                                          cTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAAct           <  2:350424/150‑1 (MQ=255)
                                                                                                                                              cTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAActcctc       >  2:1398527/1‑150 (MQ=255)
                                                                                                                                                   ggtTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTg  >  1:654612/1‑150 (MQ=255)
                                                                                                                                                   ggtTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTg  >  1:1352378/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTG  >  NC_000913/2024460‑2024756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: