Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,024,612 C→A 5.1% intergenic (+21/+23) rcsA → / ← dsrB transcriptional regulator of colanic acid capsular biosynthesis/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,024,6120CA 5.1% 395.9 / 5.1 126intergenic (+21/+23)rcsA/dsrBtranscriptional regulator of colanic acid capsular biosynthesis/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (61/57);  new base A (5/2);  total (66/60)
Fisher's exact test for biased strand distribution p-value = 4.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

GCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAG  >  NC_000913/2024463‑2024760
                                                                                                                                                     |                                                                                                                                                    
gcaaaGACCGTTTCATCGCATATAGGTAATATTTAACGTAAGATCAAAACGCATAATAAACAGGTTATCTTCCATGTCGTCCGTCTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTc                                                                                                                                                      <  1:1041690/147‑1 (MQ=255)
  cAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTccc                                                                                                                                                    <  2:1345474/150‑1 (MQ=255)
   aaGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCcca                                                                                                                                                   <  2:1712534/150‑1 (MQ=255)
     gACCGGTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGGGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCccacc                                                                                                                                                 <  2:470749/150‑1 (MQ=255)
       ccGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAg                                                                                                                                               >  2:1674660/1‑150 (MQ=255)
        cGTATCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGt                                                                                                                                              <  2:429254/150‑1 (MQ=255)
         gTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTc                                                                                                                                             <  1:268171/150‑1 (MQ=255)
         gTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGGAAACCACCAGTc                                                                                                                                             <  1:1484433/150‑1 (MQ=255)
              aTCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCt                                                                                                                                        >  2:475713/1‑150 (MQ=255)
              aTCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCt                                                                                                                                        <  2:349927/150‑1 (MQ=255)
              aTCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCt                                                                                                                                        >  2:818252/1‑150 (MQ=255)
               tCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTg                                                                                                                                       <  2:1011832/150‑1 (MQ=255)
               tCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGGAAACCACCAGTCAgaatg                                                                                                                                       <  2:1085770/150‑5 (MQ=255)
               acGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGATATCTACCATGGCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTg                                                                                                                                       <  2:463110/149‑1 (MQ=255)
                cGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACAAGCGCTAACACATTCTAACTGGTGGTTTCCCACCAGTCAGGCTGa                                                                                                                                      >  2:1299916/1‑150 (MQ=255)
                 gCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGaa                                                                                                                                     >  1:719558/1‑150 (MQ=255)
                     aaaGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAg                                                                                                                                 >  1:1452666/1‑150 (MQ=255)
                     aaaGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTACCACCAGTCAGGCTGAAAAAg                                                                                                                                 >  1:352642/1‑150 (MQ=255)
                       aGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTAAAATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTAAACATGCGATAACACATTATGACTGGTGGTTTCCGACAAGTCAGGCTGATTAAAAt                                                                                                                               >  1:32580/1‑150 (MQ=255)
                        ggTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGAtt                                                                                                                              >  2:1505863/1‑150 (MQ=255)
                          tAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTAc                                                                                                                            <  1:963684/150‑1 (MQ=255)
                            atatTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTActc                                                                                                                          <  2:278870/150‑1 (MQ=255)
                              atTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTg                                                                                                                        >  2:898158/1‑150 (MQ=255)
                              atTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTAACATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTg                                                                                                                        >  2:1000782/1‑150 (MQ=255)
                                tAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCt                                                                                                                      >  2:437711/1‑150 (MQ=255)
                                        aGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCcac                                                                                                              <  2:832346/150‑1 (MQ=255)
                                        aGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCAACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCcac                                                                                                              >  1:1021820/1‑150 (MQ=255)
                                         gATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCcaca                                                                                                             >  1:350424/1‑150 (MQ=255)
                                          aTCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACaa                                                                                                            >  2:1463407/1‑150 (MQ=255)
                                             aaaaCGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGa                                                                                                         <  1:1674660/150‑1 (MQ=255)
                                               aaCGCATAATAAACTGGTTATGTACCATGTGGTCCGAGTCACGTATAATGTGACTAATGGTATTTTTGTCAAAATGCGCTAACCCATTCTGACTGGTGGTTTCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTATCCACAAAGATAc                                                                                                      <  1:295850/150‑1 (MQ=255)
                                                  gCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCg                                                                                                    <  2:719558/150‑1 (MQ=255)
                                                   cATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTAAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTAAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGt                                                                                                   >  1:143589/1‑150 (MQ=255)
                                                     taataaACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGTTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTcc                                                                                                 >  2:1204014/1‑150 (MQ=255)
                                                     taataaACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAGGATACCGTcc                                                                                                 >  1:1291220/1‑150 (MQ=255)
                                                     taataaACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACAATCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTTCTATCTCCACAAAGATACCGTcc                                                                                                 >  2:2200/1‑150 (MQ=255)
                                                      aataaACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGCTTACTCTTCTTTCTCCACAAAGATACCGTCCt                                                                                                >  2:1142540/1‑150 (MQ=255)
                                                      aataaACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCt                                                                                                >  2:467747/1‑150 (MQ=255)
                                                         aaaCAGGTTATCTACCATGTCGTCCCACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCCCCAGTCAGTATGAATAAGATTACTATGCTTACTCCACAAATCTACACTCCTGAt                                                                                             >  2:605258/1‑150 (MQ=255)
                                                          aaCAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATg                                                                                            <  2:1365284/150‑1 (MQ=255)
                                                             aGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGccc                                                                                         <  2:857904/150‑1 (MQ=255)
                                                                ttATCTACCATGTCGTCCGACTGACTGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGTTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGc                                                                                      <  2:534448/150‑1 (MQ=255)
                                                                 tATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCt                                                                                     <  1:1310380/150‑1 (MQ=255)
                                                                   tCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCCGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTc                                                                                   >  1:455438/1‑150 (MQ=255)
                                                                    cTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCa                                                                                  <  2:112220/150‑1 (MQ=255)
                                                                     tACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCAt                                                                                 >  2:16625/1‑150 (MQ=255)
                                                                      aCCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCAtt                                                                                >  2:1561645/1‑150 (MQ=255)
                                                                      aCCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCAtt                                                                                <  2:1720709/150‑1 (MQ=255)
                                                                        cATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTaa                                                                              <  2:924207/150‑1 (MQ=255)
                                                                          tGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAg                                                                            <  1:669822/150‑1 (MQ=255)
                                                                           gtcgtcCGACTGACGGATAATGTGACTAATGGTAATTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCCTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGa                                                                           <  1:1148012/150‑1 (MQ=255)
                                                                            tcgtcCGACTGACGGAAAATGTGACTATTCGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGAGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTCCTGTGTCATCAAAGATACCGTCCTGATGCCCTGATGAATTAAAGaa                                                                          >  1:273452/1‑150 (MQ=255)
                                                                             cgtcCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAAc                                                                         >  2:730710/1‑150 (MQ=255)
                                                                             cgtcCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAAc                                                                         >  2:1212189/1‑150 (MQ=255)
                                                                              gtcCGACTGACTGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAAcc                                                                        <  2:495096/150‑1 (MQ=255)
                                                                              gtcCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAAcc                                                                        <  2:577089/150‑1 (MQ=255)
                                                                              gtcCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTATGCTTTCTCCAAAAAGATACCGTCCTGATGCACTGCTTCATTAAAGAAca                                                                        >  1:1074518/1‑149 (MQ=255)
                                                                                ccGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAg                                                                      <  2:735289/150‑1 (MQ=255)
                                                                                    cTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGc                                                                  <  1:475336/150‑1 (MQ=255)
                                                                                    cTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGGCCTGATGCCCTGCTTCATTAAAGAACCAGATGc                                                                  >  1:1395666/1‑150 (MQ=255)
                                                                                      gACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCg                                                                <  2:527581/150‑1 (MQ=255)
                                                                                      gACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCg                                                                >  1:232460/1‑150 (MQ=255)
                                                                                      gACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCg                                                                <  1:437711/150‑1 (MQ=255)
                                                                                          gATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCCCCAGTCAGGCTGAATGAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCg                                                            >  2:824385/1‑150 (MQ=255)
                                                                                          gATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCg                                                            <  1:1245836/150‑1 (MQ=255)
                                                                                           aTAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCgg                                                           <  1:630663/150‑1 (MQ=255)
                                                                                            tAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCggg                                                          <  1:1600071/150‑1 (MQ=255)
                                                                                               tgtgACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAg                                                       <  1:1561645/150‑1 (MQ=255)
                                                                                               tgtgACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGGGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATTCCGAGCGGGTAg                                                       <  1:827883/150‑1 (MQ=255)
                                                                                                gtgACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGACACAGATGCCGAGCGGGTAGt                                                      >  2:342217/1‑150 (MQ=255)
                                                                                                 tgACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTc                                                     >  1:1390063/1‑150 (MQ=255)
                                                                                                  taCTAATGGTATTTTTGTCAAAATGCGCTAACACATTCTGACTTGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCt                                                    <  1:1078010/149‑1 (MQ=255)
                                                                                                  gACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCt                                                    <  2:1002333/150‑1 (MQ=255)
                                                                                                   aCTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCtt                                                   <  1:1525741/150‑1 (MQ=255)
                                                                                                      aaTGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCa                                                >  2:829581/1‑150 (MQ=255)
                                                                                                        tGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGc                                              <  2:1381676/150‑1 (MQ=255)
                                                                                                          gTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGa                                            >  1:1376043/1‑150 (MQ=255)
                                                                                                            aTTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGaaa                                          >  2:1488042/1‑150 (MQ=255)
                                                                                                             tttttGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAAc                                         >  1:1717749/1‑150 (MQ=255)
                                                                                                             tttttGTCAACATGCGCTAACACATTCTGACTGGTGGTGTCCCACCAGGCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTGCATTAAAGAACCAGATGCCGAGCGGGGAGTCTTCCAGCGAAAc                                         >  2:1713954/1‑150 (MQ=255)
                                                                                                               tttGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCa                                       <  2:498648/150‑1 (MQ=255)
                                                                                                                  gTCAACATGAGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCATTCTCCACAAAGATACCGTCCTGCTGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAACCCAGGt                                    >  2:1589873/1‑150 (MQ=255)
                                                                                                                    cAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTAc                                  >  2:1145299/1‑150 (MQ=255)
                                                                                                                    cAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTAc                                  >  2:1011492/1‑150 (MQ=255)
                                                                                                                        aTGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATtg                              <  1:1532331/150‑1 (MQ=255)
                                                                                                                           cgcTAACACATTCTGACTGGTGGGTTCCCACCAGTCAGGCGGAATTAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGGTTCATTAAAGAACCAGCTGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGc                           <  2:273452/150‑1 (MQ=255)
                                                                                                                               aaCACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGATACCAGGTACATTGTGCCTTc                       >  1:774817/1‑150 (MQ=255)
                                                                                                                               aaCACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTc                       >  2:1662743/1‑150 (MQ=255)
                                                                                                                                   caTTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTa                   <  1:475713/150‑1 (MQ=255)
                                                                                                                                     gccTGACTGGTGGGAAACCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTaca                 >  1:57378/3‑148 (MQ=255)
                                                                                                                                     gccTGACTGGTGGGAAACCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTaaa                 >  1:6332/3‑150 (MQ=255)
                                                                                                                                      tCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAAc                >  2:1602258/1‑150 (MQ=255)
                                                                                                                                      ccTGACTGGTGGGAAACCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAAc                >  1:669770/2‑150 (MQ=255)
                                                                                                                                       cTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAACct               >  2:1548778/1‑150 (MQ=255)
                                                                                                                                       cTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAAct               <  2:350424/150‑1 (MQ=255)
                                                                                                                                       cTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAAct               >  1:1277305/1‑150 (MQ=255)
                                                                                                                                           cTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAActcctc           >  2:1655184/1‑150 (MQ=255)
                                                                                                                                           cTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAActcctc           >  2:1398527/1‑150 (MQ=255)
                                                                                                                                                ggtTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTg      >  1:1352378/1‑150 (MQ=255)
                                                                                                                                                ggtTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTg      >  1:654612/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGCCAG  >  NC_000913/2024463‑2024760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: