Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,032,336 G→C 7.9% intergenic (‑19/+48) dcm ← / ← yedJ DNA cytosine methyltransferase/putative HD superfamily phosphohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,032,3360GC 7.9% 349.1 / 16.7 113intergenic (‑19/+48)dcm/yedJDNA cytosine methyltransferase/putative HD superfamily phosphohydrolase
Reads supporting (aligned to +/- strand):  ref base G (59/45);  new base C (6/3);  total (65/48)
Fisher's exact test for biased strand distribution p-value = 7.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.31e-01

Only 100 of 114 total aligned reads displayed.

GATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAAT  >  NC_000913/2032188‑2032484
                                                                                                                                                    |                                                                                                                                                    
gATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTga                                                                                                                                                     >  2:1114589/1‑150 (MQ=255)
      cACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGc                                                                                                                                               >  2:1604869/1‑150 (MQ=255)
      cACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGc                                                                                                                                               <  1:1345520/150‑1 (MQ=255)
       aCCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAATTTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGcc                                                                                                                                              <  2:1529295/150‑1 (MQ=255)
          tACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTCAGATGGCCggt                                                                                                                                           <  1:1291974/150‑3 (MQ=255)
           acacCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACa                                                                                                                                          >  2:91392/1‑150 (MQ=255)
            cacCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTCAGGTGGCCggtga                                                                                                                                         <  2:557184/150‑5 (MQ=255)
            cacCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTCAGATGGCCggtga                                                                                                                                         <  1:1333191/150‑5 (MQ=255)
              ccATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGt                                                                                                                                       >  1:1149983/1‑150 (MQ=255)
              ccATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGt                                                                                                                                       >  1:664371/1‑150 (MQ=255)
              ccATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGt                                                                                                                                       >  2:1474838/1‑150 (MQ=255)
               cATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGtt                                                                                                                                      >  2:754140/1‑150 (MQ=255)
               cATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGtt                                                                                                                                      >  2:747690/1‑150 (MQ=255)
                  tAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAAc                                                                                                                                   >  2:352541/1‑150 (MQ=255)
                    aGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACAt                                                                                                                                 <  1:1231255/150‑1 (MQ=255)
                    aGCTGCCCCACCAACGTATTAACATCAAAAATTAGCAGCAGTTTCACCAGCATTGCCTGAGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACAt                                                                                                                                 <  1:1141767/150‑1 (MQ=255)
                     gCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATa                                                                                                                                <  2:16165/150‑1 (MQ=255)
                     gCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATa                                                                                                                                <  2:621253/150‑1 (MQ=255)
                       tGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAAt                                                                                                                              >  1:943371/1‑150 (MQ=255)
                            caccaACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAg                                                                                                                         <  2:1323301/150‑1 (MQ=255)
                            caccaACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAg                                                                                                                         <  2:1638414/150‑1 (MQ=255)
                             accaACGTTTTAACATCATAAATTTGCCGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGc                                                                                                                        <  2:1452118/150‑1 (MQ=255)
                             accaACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGc                                                                                                                        <  2:1458836/150‑1 (MQ=255)
                             accaACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGc                                                                                                                        <  1:993816/150‑1 (MQ=255)
                              ccaACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGcc                                                                                                                       >  2:1345284/1‑150 (MQ=255)
                               caACGTTTTAACATCATAAATTTGCAGCAGTTTCTCAAGCATTGCCTGAGCGGCATTACCGGTCCTGTATGAATAGGTAACTGATATATTTTCCTGCATAGATTTAACCTGCCATCTGAGATGGACTACATATTAAAATAATTACAGCCa                                                                                                                      >  2:1457698/1‑150 (MQ=255)
                                  cGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCAttt                                                                                                                   >  1:40093/1‑150 (MQ=255)
                                     tttAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAgg                                                                                                                >  1:157332/1‑150 (MQ=255)
                                     tttAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAgg                                                                                                                <  1:282939/150‑1 (MQ=255)
                                       tAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGc                                                                                                              <  1:1447002/150‑1 (MQ=255)
                                             catAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTAGAGAGATATTTTCCGTCATAGATTTCACCGGCCATCTGAGATGGACGACATTTTAACATAATTACAGCCATTTTGGGCCAcgcg                                                                                                        >  2:231650/1‑150 (MQ=255)
                                             catAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCAcgcg                                                                                                        >  2:618282/1‑150 (MQ=255)
                                               tAAATTTGCAGCAGTTTCTCTAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCt                                                                                                      <  1:664386/150‑1 (MQ=255)
                                                  aTTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTcag                                                                                                   <  1:1671143/150‑1 (MQ=255)
                                                       cagcagTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTcagccagc                                                                                              >  1:1603979/1‑150 (MQ=255)
                                                       cagcagTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTcagccagc                                                                                              >  2:1713675/1‑150 (MQ=255)
                                                        agcagTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCg                                                                                             <  1:1550607/150‑1 (MQ=255)
                                                        agcagTTTCTCCAGCATTGCCGGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCg                                                                                             <  1:868694/150‑1 (MQ=255)
                                                         gcagTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCgg                                                                                            <  1:756873/150‑1 (MQ=255)
                                                          cagTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGa                                                                                           <  1:986432/150‑1 (MQ=255)
                                                           agTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGAt                                                                                          <  1:1511975/150‑1 (MQ=255)
                                                            gTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATg                                                                                         <  2:28945/150‑1 (MQ=255)
                                                            gTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATg                                                                                         >  1:1712281/1‑150 (MQ=255)
                                                            gTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATg                                                                                         >  1:1334361/1‑150 (MQ=255)
                                                                  ccAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATCGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACg                                                                                   >  1:827870/1‑150 (MQ=255)
                                                                  ccAGCATTGCCTGTGCGGCATTCCCGGTGCTGTAGGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCAGACAGGTTAACATACTTACAGCCATTTAGGGCCACGCGCTCAGCCCGCggaggacaccg                                                                                   >  1:1410923/1‑145 (MQ=255)
                                                                     gCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCAt                                                                                >  2:1314070/1‑150 (MQ=255)
                                                                     gCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCAt                                                                                >  1:173657/1‑150 (MQ=255)
                                                                       aTTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGCCAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCt                                                                              >  2:1346830/1‑150 (MQ=255)
                                                                       aTTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCt                                                                              >  1:1440324/1‑150 (MQ=255)
                                                                          gCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTAtg                                                                           <  1:91392/150‑2 (MQ=255)
                                                                          gCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATc                                                                           >  1:751707/1‑150 (MQ=255)
                                                                          gCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATc                                                                           >  1:33182/1‑150 (MQ=255)
                                                                              gtgCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCt                                                                       >  2:576251/1‑150 (MQ=255)
                                                                                gCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTtg                                                                     <  2:861202/150‑1 (MQ=255)
                                                                                 cGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTtgt                                                                    >  1:925627/1‑150 (MQ=255)
                                                                                  ggCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTtgtg                                                                   <  1:1307230/150‑1 (MQ=255)
                                                                                  ggCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTtgtg                                                                   <  1:717072/150‑1 (MQ=255)
                                                                                       tCCCGGTGCTGTATGAATAGTTTTCTGATATATTTTCCTCCATAGATTTCACCGGCCATCTGATATGGCCGACAGGTTAACATAATTACCGGCATTTTGGGCTAAGCGCTCAGCCATCGGATGAAAACGCATCTAGCACCTTGTGATcta                                                              <  2:464652/150‑3 (MQ=255)
                                                                                           ggTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACAcc                                                          >  2:845377/1‑150 (MQ=255)
                                                                                                 gTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCAtt                                                    >  2:396452/1‑150 (MQ=255)
                                                                                                 gTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCAtt                                                    <  2:157332/150‑1 (MQ=255)
                                                                                                 gTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGAAATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCAACTATCACCTTGTGCTCGACACCTTCAta                                                    >  2:132724/1‑149 (MQ=255)
                                                                                                    tGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATtctc                                                 >  2:1579840/1‑150 (MQ=255)
                                                                                                      aaTCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTccc                                               >  1:1000252/1‑150 (MQ=255)
                                                                                                         cGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTcccccc                                            >  2:1046644/1‑149 (MQ=255)
                                                                                                          ggTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGc                                           >  2:205673/1‑150 (MQ=255)
                                                                                                          ggTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGc                                           >  1:458602/1‑150 (MQ=255)
                                                                                                          ggTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGc                                           >  1:762528/1‑150 (MQ=255)
                                                                                                             tACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAg                                        >  1:1350240/1‑150 (MQ=255)
                                                                                                               cTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGtt                                      >  2:223813/1‑150 (MQ=255)
                                                                                                                tGATATATTTTCCTGCATAGATTTCACCGGCCATATGAGATGGACGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTc                                     >  1:1323826/1‑150 (MQ=255)
                                                                                                                 gATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGCCAGGTTAACATAATTACAGCCATTTAGGGCCCCGCGCTCCGCCAGCGGATGAACCCGCATCTATCACCTTGTGATCGCCCCCTGCATTCTCCCCCGCCAGTTCg                                    >  1:79779/1‑150 (MQ=255)
                                                                                                                  atatatTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCgg                                   <  1:757997/150‑1 (MQ=255)
                                                                                                                     tatTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATACTTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCAc                                <  1:1522574/150‑1 (MQ=255)
                                                                                                                     tatTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCAc                                >  1:1678520/1‑150 (MQ=255)
                                                                                                                            cTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCtt                         >  2:787299/1‑150 (MQ=255)
                                                                                                                            cTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCtt                         >  1:21951/1‑150 (MQ=255)
                                                                                                                              gCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCg                       >  2:1450020/1‑150 (MQ=255)
                                                                                                                              gCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCg                       >  2:1128464/1‑150 (MQ=255)
                                                                                                                               cATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGc                      <  1:396452/150‑1 (MQ=255)
                                                                                                                               cATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGc                      >  2:1186945/1‑150 (MQ=255)
                                                                                                                               cATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGc                      >  1:1170643/1‑150 (MQ=255)
                                                                                                                                aTAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGcc                     <  1:1114589/150‑1 (MQ=255)
                                                                                                                                aTAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGcc                     <  1:1604869/150‑1 (MQ=255)
                                                                                                                                aTAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGcc                     >  1:1193349/1‑150 (MQ=255)
                                                                                                                                 tAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCa                    <  1:747690/150‑1 (MQ=255)
                                                                                                                                   gATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACCCCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATa                  >  1:15653/1‑150 (MQ=255)
                                                                                                                                    aacctgtcGGCCATCTCAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCCCGCGCTCAGCCAGCGGATGAAAACGCATCTATCGCCTTGTGATCGCCACCTTCATTCTCCCCCGCCAGGTCGGCACTGAGCTTCGCCATaa                 >  1:805923/8‑150 (MQ=255)
                                                                                                                                       ctgtcGGCCATCTCAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCCTCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACt              >  2:267682/5‑150 (MQ=255)
                                                                                                                                       ctgtcGGCCATCTCAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACt              >  1:1130263/5‑150 (MQ=255)
                                                                                                                                         gtcGGCCATCTCAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCg            >  1:421351/3‑150 (MQ=255)
                                                                                                                                         gtcGGCCATCTCAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACCGAGCTTCGCCATAAACTCg            >  1:967925/3‑150 (MQ=255)
                                                                                                                                          ccGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGa           >  2:557405/1‑150 (MQ=255)
                                                                                                                                           cGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGAc          <  2:1149983/150‑1 (MQ=255)
                                                                                                                                            ggCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACt         <  1:845377/150‑1 (MQ=255)
                                                                                                                                            ggCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACt         <  1:414359/150‑1 (MQ=255)
                                                                                                                                                aTCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTAAGCCAGCTGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTaaaa     <  2:943371/150‑1 (MQ=255)
                                                                                                                                                  cTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTaaaaaa   <  2:421357/150‑1 (MQ=255)
                                                                                                                                                   tGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTaaaaact  >  1:506671/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAAT  >  NC_000913/2032188‑2032484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: