Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,041,166 G→T 6.7% intergenic (+48/‑209) yedZ → / → zinT inner membrane heme subunit for periplasmic YedYZ reductase/zinc and cadmium binding protein, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,041,1660GT 6.7% 358.9 / 10.2 139intergenic (+48/‑209)yedZ/zinTinner membrane heme subunit for periplasmic YedYZ reductase/zinc and cadmium binding protein, periplasmic
Reads supporting (aligned to +/- strand):  ref base G (62/65);  new base T (4/6);  total (68/71)
Fisher's exact test for biased strand distribution p-value = 7.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.58e-01

Only 100 of 141 total aligned reads displayed.

GCTGGGCTGGCTGTACTGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCAT  >  NC_000913/2041020‑2041313
                                                                                                                                                  |                                                                                                                                                   
gctgggctggCTGTACTGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAgc                                                                                                                                                  <  2:47187/150‑1 (MQ=255)
   gggctggCTGTACTGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGcc                                                                                                                                               <  1:1446286/150‑1 (MQ=255)
    ggctggctGTACTGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGccc                                                                                                                                              >  1:720393/1‑150 (MQ=255)
        ggctgTACTGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATc                                                                                                                                          >  1:25456/1‑150 (MQ=255)
         gctgTACTGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATcc                                                                                                                                         >  1:669302/1‑150 (MQ=255)
         gctgTACTGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATAGGGCGCTCGCGCCACATcc                                                                                                                                         <  1:156056/150‑1 (MQ=255)
         gctgTACTGCTTTTAGCCTTACGGTATAAGAAGTTGAGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATATGTATAACTTCATATTGCCGACAAAGTACGCGTAATCCCTGCCGGATGTGGCGCAAGCGCCCTATcc                                                                                                                                         >  1:881380/1‑150 (MQ=255)
         gctgTACTGCTTTTAGCCTTACGGTAAAAGAAGTTGCGGTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATcc                                                                                                                                         <  1:121644/150‑1 (MQ=255)
         gctgTACTGCTTTTAGCCTGACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGATACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTAATATTGCCGACAAAGTACGCGTTATCCCTGCCAGATGTGGCGCGAGCGCCCTATcc                                                                                                                                         >  2:1103492/1‑150 (MQ=255)
          ctttACTGCTTAAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGGTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCgg                                                                                                                                       <  1:81717/147‑1 (MQ=255)
           tgTACTGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCgg                                                                                                                                       <  2:205237/150‑1 (MQ=255)
           tgTACTGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCgg                                                                                                                                       <  1:1342148/150‑1 (MQ=255)
             tACTGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCa                                                                                                                                     >  1:1259527/1‑150 (MQ=255)
              acggcgtttAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTAAACCGGTTACGCAAGCAGGGTCACAACAAATTATCTGTATAACTTCATATTCCCGACAAAGTCCGCGTTATCCCTGCCGCATAGGGCGCTCGCGCCACATCCGGCag                                                                                                                                    <  1:1652290/144‑2 (MQ=255)
              aCTGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCAt                                                                                                                                    >  2:1255198/1‑150 (MQ=255)
               cTGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCGAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATAGGGCGCTCGCGCCACATCCGGCagg                                                                                                                                   <  2:107961/150‑3 (MQ=255)
                tGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATac                                                                                                                                  >  2:177417/1‑150 (MQ=255)
                  cTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATacac                                                                                                                                <  1:500138/150‑1 (MQ=255)
                    tttAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGAGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAg                                                                                                                              <  1:926896/150‑1 (MQ=255)
                     ttAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGa                                                                                                                             <  1:1699435/150‑1 (MQ=255)
                       aGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGAta                                                                                                                           >  1:1607836/1‑149 (MQ=255)
                        gCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCg                                                                                                                          >  1:1303101/1‑150 (MQ=255)
                          cTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGCGCGCCCTATCCGGCATACCCAGATCGcc                                                                                                                        >  2:226533/1‑149 (MQ=255)
                          cTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCa                                                                                                                        <  2:720393/150‑1 (MQ=255)
                            tACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATc                                                                                                                      <  2:1107871/150‑1 (MQ=255)
                               ggTATAAGAAGTTGCGTTCCCTTTTTAACCGGCTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAgg                                                                                                                   <  1:997137/150‑1 (MQ=255)
                                  ataAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCATGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCATGTTa                                                                                                                <  2:452767/150‑1 (MQ=255)
                                  ataAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTa                                                                                                                >  2:514910/1‑150 (MQ=255)
                                  ataAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTa                                                                                                                >  1:1300984/1‑150 (MQ=255)
                                   taAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTAt                                                                                                               <  1:821913/150‑1 (MQ=255)
                                   taAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTAt                                                                                                               >  1:963506/1‑150 (MQ=255)
                                    aagaagTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATg                                                                                                              >  2:964689/1‑150 (MQ=255)
                                     agaagTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGt                                                                                                             >  1:1690589/1‑150 (MQ=255)
                                        agTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTc                                                                                                          <  1:1560506/150‑1 (MQ=255)
                                        agTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTc                                                                                                          >  2:1395103/1‑150 (MQ=255)
                                         gTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCg                                                                                                         >  1:851917/1‑150 (MQ=255)
                                          ttGAGTGCCCTTTTTAACCGGTTACGAAAGCAGTTTCACAAAAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGGGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGt                                                                                                        <  1:717638/150‑1 (MQ=255)
                                            gCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGttt                                                                                                      >  1:1019101/1‑150 (MQ=255)
                                              gTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGt                                                                                                    <  2:701807/150‑1 (MQ=255)
                                              gTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGt                                                                                                    >  1:758464/1‑150 (MQ=255)
                                               ttCCCTTATTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGtc                                                                                                   <  1:1409823/150‑1 (MQ=255)
                                                  ccTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGtcatc                                                                                                >  2:615052/1‑150 (MQ=255)
                                                    tttttAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCaa                                                                                              <  1:366722/150‑1 (MQ=255)
                                                    tttttAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCaa                                                                                              >  1:206378/1‑150 (MQ=255)
                                                              gTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCa                                                                                    <  2:822118/150‑1 (MQ=255)
                                                                  cGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCaa                                                                                >  2:1236850/1‑150 (MQ=255)
                                                                  cGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCaa                                                                                >  2:1530772/1‑150 (MQ=255)
                                                                    cAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCaaaa                                                                              >  1:271329/1‑150 (MQ=255)
                                                                        cAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAaataat                                                                          <  2:1405176/150‑1 (MQ=255)
                                                                        cAGGTTAACAACAAATTATATGTATAACTTCATATTGCCGACAAAGTAAGCGTTATAACTGCCGGATGTGGCGCGAGCGCCATATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAaataat                                                                          >  1:671098/1‑150 (MQ=255)
                                                                         aGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAatt                                                                         <  1:1255198/150‑1 (MQ=255)
                                                                          ggTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAattt                                                                        <  1:301262/150‑1 (MQ=255)
                                                                          ggTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAattt                                                                        <  1:514910/150‑1 (MQ=255)
                                                                           gTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAattta                                                                       >  1:1674016/1‑150 (MQ=255)
                                                                             tCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAatttatt                                                                     <  2:397825/150‑1 (MQ=255)
                                                                                    aaaTTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCTGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTc                                                              >  1:397192/1‑150 (MQ=255)
                                                                                    aaaTTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTc                                                              <  1:862383/150‑1 (MQ=255)
                                                                                          tCTGTATTACTTCATATTGCCGACATAGTACGCGTTATCCCTGCCGGATGTGGCGAGAGCTCAATATCCGGCATACACAGATCGCATAAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAag                                                        <  1:300194/150‑1 (MQ=255)
                                                                                          tCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAag                                                        <  1:1134219/150‑1 (MQ=255)
                                                                                            tGTATAACTTCTTATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAagat                                                      <  1:842188/150‑1 (MQ=255)
                                                                                            tGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAagat                                                      <  2:807981/150‑1 (MQ=255)
                                                                                             gTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAagata                                                     <  1:337175/150‑1 (MQ=255)
                                                                                                taACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAagatagat                                                  >  2:245217/1‑150 (MQ=255)
                                                                                                 aaCTTCATTTTGCCGACAAAGTACGCTTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAgatagata                                                 <  2:212068/150‑1 (MQ=255)
                                                                                                 aaCTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAgatagata                                                 >  2:511090/1‑150 (MQ=255)
                                                                                                  aCTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATaa                                                >  1:968681/1‑150 (MQ=255)
                                                                                                  aCTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATaa                                                >  1:1085116/1‑150 (MQ=255)
                                                                                                         atTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAAc                                         <  2:1031096/150‑1 (MQ=255)
                                                                                                           tGCCGACAAAGTACTCGTTATACCTTCCGTATGTGGCGCTAGCGACCTATACGGCATAAACAGATAGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCTAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTg                                       <  1:273470/150‑1 (MQ=255)
                                                                                                           tGCCGACAAAGTACGCGTTATCCATGACGGATTTTTATAGAGCTCCCTATCCGGCATAAACAGATCTCATCAGTTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGaaacg                                       <  1:226533/150‑4 (MQ=255)
                                                                                                             ccGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTgag                                     >  1:777084/1‑150 (MQ=255)
                                                                                                             ccGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTgag                                     <  2:861168/150‑1 (MQ=255)
                                                                                                              cGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCCTCAGGTTATGTTTCGTTTGTCATCAACCTCTAATCACCAAAATAATTTATTTTATTTCAATTAGATAGATAAGTAGAACTgaga                                    >  1:872798/1‑150 (MQ=255)
                                                                                                              cGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTgaga                                    >  2:850432/1‑150 (MQ=255)
                                                                                                                aCAAAGTACGCGTTATCCCTGCCGGATGTTGCGCGAGCGCCCTATCCGTCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGaaa                                  <  1:1005783/150‑1 (MQ=255)
                                                                                                                  aaaGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGc                                <  2:1259527/150‑1 (MQ=255)
                                                                                                                   aaGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGcc                               <  1:1019939/150‑1 (MQ=255)
                                                                                                                     gTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCAt                             >  2:1461395/1‑150 (MQ=255)
                                                                                                                          cgTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGACATGctct                        >  1:1263683/1‑150 (MQ=255)
                                                                                                                             tATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGt                     <  2:1607836/150‑1 (MQ=255)
                                                                                                                                cccTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTc                  >  2:1296248/1‑150 (MQ=255)
                                                                                                                                cccTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTc                  <  1:1579226/150‑1 (MQ=255)
                                                                                                                                 cccgCCGGATGTGGCGCGAGCTCCCTATCCGGCATACACAGATCTCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTCTTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTGcc                 <  2:848043/147‑1 (MQ=255)
                                                                                                                                 ccTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTcc                 <  2:1296171/150‑1 (MQ=255)
                                                                                                                                 ccTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTcc                 <  2:1303101/150‑1 (MQ=255)
                                                                                                                                  cTGCAGGATTTGGCGCGAGCGCACTATACTGAATACAAAGATAGCATAATGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCt                <  1:236909/150‑1 (MQ=255)
                                                                                                                                  cTGACGGATGTGGCGCTAGAGCACTATCCGGCATACACAGATCGCATTAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCATAATAATTTATTTTATTTCAATAAGATAGATAATTAGAACTGAGAAAGCCATGCTCTCGTTTCCt                <  2:881380/150‑1 (MQ=255)
                                                                                                                                  atGCCGGATAGGGCGCTCGCGCCACATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCt                >  2:703536/2‑150 (MQ=255)
                                                                                                                                   tGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTAAAATCACCAAAATAAATTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTa               <  2:672105/150‑1 (MQ=255)
                                                                                                                                    gCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCGCAAATCACCAAAATAATTTATTTTATTTCATTAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCATaa              >  1:861365/1‑150 (MQ=255)
                                                                                                                                     ccGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAag             <  1:615052/150‑1 (MQ=255)
                                                                                                                                     ccGGATAGGGCGCTCGCGCCACATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAag             >  1:1238563/1‑150 (MQ=255)
                                                                                                                                     ccGGATAGGGCGCTCGCGCCACATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAag             >  2:1619531/1‑150 (MQ=255)
                                                                                                                                      cGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAaga            <  2:834902/150‑1 (MQ=255)
                                                                                                                                       ggatagggCGCTCGCGCCACATCCGGCATACACAGATCGCATCAGGTTATGTTTAGTTTGTCATAAACCTCAAATCACCAAAAAAATTTATTTTATTTCAATAAGATAGATTAGTAGAACTGAGAAAGCGATGCTCTCGTTTCCTAagag           >  2:988727/7‑150 (MQ=255)
                                                                                                                                          tgtgGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAgttg        >  1:1550354/1‑150 (MQ=255)
                                                                                                                                           gtgGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAgttgt       >  1:802691/1‑150 (MQ=255)
                                                                                                                                             ggCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGttgttg     <  2:561914/150‑1 (MQ=255)
                                                                                                                                              gcgcGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATATTATTTCAAAAAGATAGATAAGTAGAACTGATAAACCCATCCTCTCGTTTCCTAAGAGTTGTTGc    >  2:184366/1‑150 (MQ=255)
                                                                                                                                               cgcgAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCCCCAAAATAATTTATTTTATTTAAATAAGATAGATAAGTAGAACTGAGAAAGCAATGATCTAGTTTCCTAAGAGTTGTTGCa   >  2:162828/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GCTGGGCTGGCTGTACTGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCAT  >  NC_000913/2041020‑2041313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: