Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,041,173 C→A 7.0% intergenic (+55/‑202) yedZ → / → zinT inner membrane heme subunit for periplasmic YedYZ reductase/zinc and cadmium binding protein, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,041,1730CA 7.0% 385.1 / 12.3 139intergenic (+55/‑202)yedZ/zinTinner membrane heme subunit for periplasmic YedYZ reductase/zinc and cadmium binding protein, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (64/64);  new base A (5/6);  total (69/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 142 total aligned reads displayed.

GGCTGGCTGTACTGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATA  >  NC_000913/2041024‑2041322
                                                                                                                                                     |                                                                                                                                                     
     gctgTACTGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATcc                                                                                                                                                  >  1:669302/1‑150 (MQ=255)
     gctgTACTGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATAGGGCGCTCGCGCCACATcc                                                                                                                                                  <  1:156056/150‑1 (MQ=255)
     gctgTACTGCTTTTAGCCTTACGGTATAAGAAGTTGAGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATATGTATAACTTCATATTGCCGACAAAGTACGCGTAATCCCTGCCGGATGTGGCGCAAGCGCCCTATcc                                                                                                                                                  >  1:881380/1‑150 (MQ=255)
     gctgTACTGCTTTTAGCCTTACGGTAAAAGAAGTTGCGGTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATcc                                                                                                                                                  <  1:121644/150‑1 (MQ=255)
     gctgTACTGCTTTTAGCCTGACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGATACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTAATATTGCCGACAAAGTACGCGTTATCCCTGCCAGATGTGGCGCGAGCGCCCTATcc                                                                                                                                                  >  2:1103492/1‑150 (MQ=255)
      ctttACTGCTTAAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGGTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCgg                                                                                                                                                <  1:81717/147‑1 (MQ=255)
       tgTACTGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCgg                                                                                                                                                <  2:205237/150‑1 (MQ=255)
         tACTGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCa                                                                                                                                              >  1:1259527/1‑150 (MQ=255)
          aCTGCATTTAGCCTTACGGTATAAGCAGATGCGTTCCCATTTTAACCGGTTACGCAAGCAGGATCACAACAAATTATCTGTATCACTTCATATTCCCGACAAAGTACGCGGTATCCCTGCCGGAAAGGGCGCTCGCGCCACATCCGGCag                                                                                                                                             <  1:825880/150‑2 (MQ=255)
             gCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATaca                                                                                                                                          >  1:558552/1‑150 (MQ=255)
              cTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATacac                                                                                                                                         <  1:500138/150‑1 (MQ=255)
                 ttAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGa                                                                                                                                      <  1:1699435/150‑1 (MQ=255)
                   aGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGAta                                                                                                                                    >  1:1607836/1‑149 (MQ=255)
                    gCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCg                                                                                                                                   >  1:1303101/1‑150 (MQ=255)
                     ccTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGc                                                                                                                                  >  1:861168/1‑150 (MQ=255)
                      cTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGCGCGCCCTATCCGGCATACCCAGATCGcc                                                                                                                                 >  2:226533/1‑149 (MQ=255)
                      cTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCa                                                                                                                                 <  2:720393/150‑1 (MQ=255)
                           ggTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACTAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAgg                                                                                                                            <  1:727796/150‑1 (MQ=255)
                           ggTATAAGAAGTTGCGTTCCCTTTTTAACCGGCTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAgg                                                                                                                            <  1:997137/150‑1 (MQ=255)
                              ataAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCATGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCATGTTa                                                                                                                         <  2:452767/150‑1 (MQ=255)
                              ataAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTa                                                                                                                         >  1:1300984/1‑150 (MQ=255)
                              ataAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTa                                                                                                                         >  2:509931/1‑150 (MQ=255)
                               taAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTAt                                                                                                                        >  1:963506/1‑150 (MQ=255)
                                    agTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTc                                                                                                                   <  1:1560506/150‑1 (MQ=255)
                                      ttGAGTGCCCTTTTTAACCGGTTACGAAAGCAGTTTCACAAAAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGGGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGt                                                                                                                 <  1:717638/150‑1 (MQ=255)
                                        gCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGttt                                                                                                               >  1:1019101/1‑150 (MQ=255)
                                         cGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTg                                                                                                              <  2:910498/150‑1 (MQ=255)
                                          gTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGt                                                                                                             <  2:701807/150‑1 (MQ=255)
                                          gTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGt                                                                                                             >  1:758464/1‑150 (MQ=255)
                                           ttCCCTTATTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGtc                                                                                                            <  1:1409823/150‑1 (MQ=255)
                                            tCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGtca                                                                                                           <  2:722689/150‑1 (MQ=255)
                                                tttttAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCaa                                                                                                       <  1:366722/150‑1 (MQ=255)
                                                tttttAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCaa                                                                                                       >  1:206378/1‑150 (MQ=255)
                                                         ggTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATc                                                                                              >  2:108814/1‑150 (MQ=255)
                                                         ggTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATc                                                                                              >  1:998944/1‑150 (MQ=255)
                                                          gTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCa                                                                                             >  2:1261162/1‑150 (MQ=255)
                                                          gTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCa                                                                                             <  2:822118/150‑1 (MQ=255)
                                                              cGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCaa                                                                                         >  2:1236850/1‑150 (MQ=255)
                                                                cAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCaaaa                                                                                       >  1:271329/1‑150 (MQ=255)
                                                                    cAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAaataat                                                                                   <  2:1405176/150‑1 (MQ=255)
                                                                     aGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAatt                                                                                  <  1:1255198/150‑1 (MQ=255)
                                                                      ggTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAattt                                                                                 <  1:301262/150‑1 (MQ=255)
                                                                       gTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAattta                                                                                >  1:1674016/1‑150 (MQ=255)
                                                                         tCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAatttatt                                                                              <  2:397825/150‑1 (MQ=255)
                                                                            caacaaATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAAtttatttta                                                                           >  2:119445/1‑150 (MQ=255)
                                                                            caacaaATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAAtttatttta                                                                           >  2:591198/1‑150 (MQ=255)
                                                                            caacaaATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCCGCATACACAGATAGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAAtatatttta                                                                           >  1:1685053/1‑150 (MQ=255)
                                                                                aaaTTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCTGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTc                                                                       >  1:397192/1‑150 (MQ=255)
                                                                                aaaTTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTc                                                                       <  1:717139/150‑1 (MQ=255)
                                                                                      tCTGTATTACTTCATATTGCCGACATAGTACGCGTTATCCCTGCCGGATGTGGCGAGAGCTCAATATCCGGCATACACAGATCGCATAAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAag                                                                 <  1:300194/150‑1 (MQ=255)
                                                                                      tCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAag                                                                 <  1:1134219/150‑1 (MQ=255)
                                                                                        tGTATAACTTCTTATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAagat                                                               <  1:842188/150‑1 (MQ=255)
                                                                                         gTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAagata                                                              <  1:337175/150‑1 (MQ=255)
                                                                                             aaCTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAgatagata                                                          >  2:511090/1‑150 (MQ=255)
                                                                                              aCTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCTATAAGATAGATaa                                                         >  1:878313/1‑150 (MQ=255)
                                                                                              aCTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATaa                                                         >  2:199819/1‑150 (MQ=255)
                                                                                              aCTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATaa                                                         >  1:1085116/1‑150 (MQ=255)
                                                                                                ttCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGt                                                       <  2:234235/150‑1 (MQ=255)
                                                                                                     atTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCACCCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAAc                                                  >  1:1622096/1‑150 (MQ=255)
                                                                                                     atTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAAc                                                  <  2:1031096/150‑1 (MQ=255)
                                                                                                      ttGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACt                                                 <  2:1650039/150‑1 (MQ=255)
                                                                                                       tGCCGACAAAGTACTCGTTATACCTTCCGTATGTGGCGCTAGCGACCTATACGGCATAAACAGATAGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCTAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTg                                                <  1:273470/150‑1 (MQ=255)
                                                                                                       tGCCGACAAAGTACGCGTTATCCATGACGGATTTTTATAGAGCTCCCTATCCGGCATAAACAGATCTCATCAGTTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGaaacg                                                <  1:226533/150‑4 (MQ=255)
                                                                                                          cGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCCTCAGGTTATGTTTCGTTTGTCATCAACCTCTAATCACCAAAATAATTTATTTTATTTCAATTAGATAGATAAGTAGAACTgaga                                             >  1:872798/1‑150 (MQ=255)
                                                                                                          cGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTgaga                                             <  1:791343/150‑1 (MQ=255)
                                                                                                          cGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTgaga                                             >  2:850432/1‑150 (MQ=255)
                                                                                                            aCAAAGTACGCGTTATCCCTGCCGGATGTTGCGCGAGCGCCCTATCCGTCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGaaa                                           <  1:1005783/150‑1 (MQ=255)
                                                                                                              aaaGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGc                                         <  2:1259527/150‑1 (MQ=255)
                                                                                                               aaGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGcc                                        <  1:1019939/150‑1 (MQ=255)
                                                                                                                 gTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCAt                                      >  2:1461395/1‑150 (MQ=255)
                                                                                                                   aCGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGc                                    <  2:823057/150‑1 (MQ=255)
                                                                                                                      cgTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGctct                                 >  1:1405671/1‑150 (MQ=255)
                                                                                                                      cgTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGctct                                 >  1:1451765/1‑150 (MQ=255)
                                                                                                                      cgTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGACATGctct                                 >  1:1263683/1‑150 (MQ=255)
                                                                                                                         tATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGt                              <  2:1607836/150‑1 (MQ=255)
                                                                                                                            cccTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTc                           <  1:1579226/150‑1 (MQ=255)
                                                                                                                             cccgCCGGATGTGGCGCGAGCTCCCTATCCGGCATACACAGATCTCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTCTTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTGcc                          <  2:848043/147‑1 (MQ=255)
                                                                                                                             ccTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTcc                          <  2:1296171/150‑1 (MQ=255)
                                                                                                                             ccTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTcc                          <  2:1303101/150‑1 (MQ=255)
                                                                                                                              cTGCAGGATTTGGCGCGAGCGCACTATACTGAATACAAAGATAGCATAATGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCt                         <  1:236909/150‑1 (MQ=255)
                                                                                                                              cTGACGGATGTGGCGCTAGAGCACTATCCGGCATACACAGATCGCATTAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCATAATAATTTATTTTATTTCAATAAGATAGATAATTAGAACTGAGAAAGCCATGCTCTCGTTTCCt                         <  2:881380/150‑1 (MQ=255)
                                                                                                                               tGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTAAAATCACCAAAATAAATTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTa                        <  2:672105/150‑1 (MQ=255)
                                                                                                                                gCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCGCAAATCACCAAAATAATTTATTTTATTTCATTAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCATaa                       >  1:861365/1‑150 (MQ=255)
                                                                                                                                 ccGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAag                      <  1:615052/150‑1 (MQ=255)
                                                                                                                                 ccGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAag                      <  2:1300984/150‑1 (MQ=255)
                                                                                                                                 ccGGATAGGGCGCTCGCGCCACATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAag                      >  1:1238563/1‑150 (MQ=255)
                                                                                                                                   ggatagggCGCTCGCGCCACATCCGGCATACACAGATCGCATCAGGTTATGTTTAGTTTGTCATAAACCTCAAATCACCAAAAAAATTTATTTTATTTCAATAAGATAGATTAGTAGAACTGAGAAAGCGATGCTCTCGTTTCCTAagag                    >  2:988727/7‑150 (MQ=255)
                                                                                                                                   ggATTTGGCGCGAGCGCCCTATCCGTCATACACAGATCTCATCAGGTTATTTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATATCAATAATATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAagag                    <  2:1570875/150‑1 (MQ=255)
                                                                                                                                       gtgGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAgttgt                >  1:802691/1‑150 (MQ=255)
                                                                                                                                       gtgGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAgttgt                <  2:851917/150‑1 (MQ=255)
                                                                                                                                         ggCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGttgttg              <  2:561914/150‑1 (MQ=255)
                                                                                                                                         ggCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGttgttg              >  1:948512/1‑150 (MQ=255)
                                                                                                                                          gcgcGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGc             <  1:245217/150‑1 (MQ=255)
                                                                                                                                          gcgcGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATATTATTTCAAAAAGATAGATAAGTAGAACTGATAAACCCATCCTCTCGTTTCCTAAGAGTTGTTGc             >  2:184366/1‑150 (MQ=255)
                                                                                                                                           cgcgAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCCCCAAAATAATTTATTTTATTTAAATAAGATAGATAAGTAGAACTGAGAAAGCAATGATCTAGTTTCCTAAGAGTTGTTGCa            >  2:162828/1‑150 (MQ=255)
                                                                                                                                            gcgAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTAATTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCAt           >  2:699116/1‑150 (MQ=255)
                                                                                                                                                 cgcCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGc      <  2:984663/150‑1 (MQ=255)
                                                                                                                                                 cgcCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATGTTGc      >  2:1529095/1‑150 (MQ=255)
                                                                                                                                                    ccTATCCGGAATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTAAAATCACCAAAATAATTTATATTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCtat   <  2:292590/150‑1 (MQ=255)
                                                                                                                                                     cTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCtata  <  1:511090/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGCTGGCTGTACTGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATA  >  NC_000913/2041024‑2041322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: