Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,041,174 T→C 5.6% intergenic (+56/‑201) yedZ → / → zinT inner membrane heme subunit for periplasmic YedYZ reductase/zinc and cadmium binding protein, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,041,1740TC 5.6% 430.0 / 10.1 141intergenic (+56/‑201)yedZ/zinTinner membrane heme subunit for periplasmic YedYZ reductase/zinc and cadmium binding protein, periplasmic
Reads supporting (aligned to +/- strand):  ref base T (65/68);  new base C (4/4);  total (69/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 142 total aligned reads displayed.

GGCTGTACTGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATAT  >  NC_000913/2041028‑2041323
                                                                                                                                                  |                                                                                                                                                     
 gctgTACTGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATcc                                                                                                                                                   >  1:669302/1‑150 (MQ=255)
 gctgTACTGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATAGGGCGCTCGCGCCACATcc                                                                                                                                                   <  1:156056/150‑1 (MQ=255)
 gctgTACTGCTTTTAGCCTTACGGTATAAGAAGTTGAGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATATGTATAACTTCATATTGCCGACAAAGTACGCGTAATCCCTGCCGGATGTGGCGCAAGCGCCCTATcc                                                                                                                                                   >  1:881380/1‑150 (MQ=255)
 gctgTACTGCTTTTAGCCTTACGGTAAAAGAAGTTGCGGTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATcc                                                                                                                                                   <  1:121644/150‑1 (MQ=255)
 gctgTACTGCTTTTAGCCTGACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGATACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTAATATTGCCGACAAAGTACGCGTTATCCCTGCCAGATGTGGCGCGAGCGCCCTATcc                                                                                                                                                   >  2:1103492/1‑150 (MQ=255)
  ctttACTGCTTAAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGGTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCgg                                                                                                                                                 <  1:81717/147‑1 (MQ=255)
   tgTACTGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCgg                                                                                                                                                 <  2:205237/150‑1 (MQ=255)
     tACTGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCa                                                                                                                                               >  1:1259527/1‑150 (MQ=255)
      aCTGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCAt                                                                                                                                              >  2:1255198/1‑150 (MQ=255)
       cTGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCGAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATAGGGCGCTCGCGCCACATCCGGCagg                                                                                                                                             <  2:107961/150‑3 (MQ=255)
         gCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATaca                                                                                                                                           >  1:558552/1‑150 (MQ=255)
          cTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATacac                                                                                                                                          <  1:500138/150‑1 (MQ=255)
            tttAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGAGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAg                                                                                                                                        <  1:926896/150‑1 (MQ=255)
             ttAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGa                                                                                                                                       <  1:1699435/150‑1 (MQ=255)
               aGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGAta                                                                                                                                     >  1:1607836/1‑149 (MQ=255)
                gCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCg                                                                                                                                    >  1:1303101/1‑150 (MQ=255)
                  cTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGCGCGCCCTATCCGGCATACCCAGATCGcc                                                                                                                                  >  2:226533/1‑149 (MQ=255)
                  cTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCa                                                                                                                                  <  2:720393/150‑1 (MQ=255)
                    tACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATc                                                                                                                                <  2:1107871/150‑1 (MQ=255)
                          ataAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCATGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCATGTTa                                                                                                                          <  2:452767/150‑1 (MQ=255)
                          ataAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTa                                                                                                                          >  2:509931/1‑150 (MQ=255)
                          ataAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTa                                                                                                                          >  1:1300984/1‑150 (MQ=255)
                           taAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTAt                                                                                                                         <  1:821913/150‑1 (MQ=255)
                           taAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTAt                                                                                                                         >  1:963506/1‑150 (MQ=255)
                                agTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTc                                                                                                                    >  2:1395103/1‑150 (MQ=255)
                                agTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTc                                                                                                                    <  1:1560506/150‑1 (MQ=255)
                                 gTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCg                                                                                                                   >  1:851917/1‑150 (MQ=255)
                                  ttGAGTGCCCTTTTTAACCGGTTACGAAAGCAGTTTCACAAAAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGGGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGt                                                                                                                  <  1:717638/150‑1 (MQ=255)
                                    gCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGttt                                                                                                                >  1:1019101/1‑150 (MQ=255)
                                     cGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTg                                                                                                               <  2:910498/150‑1 (MQ=255)
                                      gTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGt                                                                                                              <  2:701807/150‑1 (MQ=255)
                                      gTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGt                                                                                                              >  1:758464/1‑150 (MQ=255)
                                       ttCCCTTATTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGtc                                                                                                             <  1:1409823/150‑1 (MQ=255)
                                        tCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGtca                                                                                                            <  2:722689/150‑1 (MQ=255)
                                            tttttAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCaa                                                                                                        <  1:366722/150‑1 (MQ=255)
                                            tttttAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCaa                                                                                                        >  1:206378/1‑150 (MQ=255)
                                                     ggTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATc                                                                                               >  1:998944/1‑150 (MQ=255)
                                                      gTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCa                                                                                              <  2:822118/150‑1 (MQ=255)
                                                      gTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCa                                                                                              >  2:1261162/1‑150 (MQ=255)
                                                          cGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCaa                                                                                          >  2:1236850/1‑150 (MQ=255)
                                                          cGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCaa                                                                                          >  2:1530772/1‑150 (MQ=255)
                                                            cAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCaaaa                                                                                        >  1:271329/1‑150 (MQ=255)
                                                                 aGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAatt                                                                                   <  1:1255198/150‑1 (MQ=255)
                                                                  ggTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAattt                                                                                  <  1:301262/150‑1 (MQ=255)
                                                                   gTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAattta                                                                                 >  1:1674016/1‑150 (MQ=255)
                                                                     tCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAatttatt                                                                               <  2:397825/150‑1 (MQ=255)
                                                                        caacaaATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAAtttatttta                                                                            >  2:591198/1‑150 (MQ=255)
                                                                        caacaaATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCCGCATACACAGATAGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAAtatatttta                                                                            >  1:1685053/1‑150 (MQ=255)
                                                                            aaaTTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCTGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTc                                                                        >  1:397192/1‑150 (MQ=255)
                                                                            aaaTTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTc                                                                        <  1:862383/150‑1 (MQ=255)
                                                                            aaaTTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTc                                                                        <  1:717139/150‑1 (MQ=255)
                                                                                  tCTGTATTACTTCATATTGCCGACATAGTACGCGTTATCCCTGCCGGATGTGGCGAGAGCTCAATATCCGGCATACACAGATCGCATAAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAag                                                                  <  1:300194/150‑1 (MQ=255)
                                                                                  tCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAag                                                                  <  1:1134219/150‑1 (MQ=255)
                                                                                    tGTATAACTTCTTATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAagat                                                                <  1:842188/150‑1 (MQ=255)
                                                                                     gTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAagata                                                               <  1:337175/150‑1 (MQ=255)
                                                                                         aaCTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAgatagata                                                           >  2:511090/1‑150 (MQ=255)
                                                                                          aCTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCTATAAGATAGATaa                                                          >  1:878313/1‑150 (MQ=255)
                                                                                          aCTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATaa                                                          >  1:968681/1‑150 (MQ=255)
                                                                                          aCTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATaa                                                          >  1:1085116/1‑150 (MQ=255)
                                                                                          aCTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATaa                                                          >  2:199819/1‑150 (MQ=255)
                                                                                            ttCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGt                                                        <  2:234235/150‑1 (MQ=255)
                                                                                                 atTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCACCCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAAc                                                   >  1:1622096/1‑150 (MQ=255)
                                                                                                 atTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAAc                                                   <  2:1031096/150‑1 (MQ=255)
                                                                                                  ttGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACt                                                  <  2:1650039/150‑1 (MQ=255)
                                                                                                   tGCCGACAAAGTACTCGTTATACCTTCCGTATGTGGCGCTAGCGACCTATACGGCATAAACAGATAGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCTAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTg                                                 <  1:273470/150‑1 (MQ=255)
                                                                                                   tGCCGACAAAGTACGCGTTATCCATGACGGATTTTTATAGAGCTCCCTATCCGGCATAAACAGATCTCATCAGTTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGaaacg                                                 <  1:226533/150‑4 (MQ=255)
                                                                                                     ccGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTgag                                               >  1:777084/1‑150 (MQ=255)
                                                                                                      cGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTgaga                                              >  2:850432/1‑150 (MQ=255)
                                                                                                        aCAAAGTACGCGTTATCCCTGCCGGATGTTGCGCGAGCGCCCTATCCGTCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGaaa                                            <  1:1005783/150‑1 (MQ=255)
                                                                                                           aaGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGcc                                         <  1:1019939/150‑1 (MQ=255)
                                                                                                             gTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCAt                                       >  2:1461395/1‑150 (MQ=255)
                                                                                                               aCGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGc                                     <  2:823057/150‑1 (MQ=255)
                                                                                                                  cgTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGctct                                  >  1:1405671/1‑150 (MQ=255)
                                                                                                                  cgTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGctct                                  >  1:1451765/1‑150 (MQ=255)
                                                                                                                  cgTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGACATGctct                                  >  1:1263683/1‑150 (MQ=255)
                                                                                                                     tATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGt                               <  2:1607836/150‑1 (MQ=255)
                                                                                                                        cccTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTc                            >  2:1296248/1‑150 (MQ=255)
                                                                                                                        cccTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTc                            <  1:1579226/150‑1 (MQ=255)
                                                                                                                         cccgCCGGATGTGGCGCGAGCTCCCTATCCGGCATACACAGATCTCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTCTTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTGcc                           <  2:848043/147‑1 (MQ=255)
                                                                                                                         ccTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTcc                           <  2:1303101/150‑1 (MQ=255)
                                                                                                                         ccTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTcc                           <  2:1296171/150‑1 (MQ=255)
                                                                                                                          cTGCAGGATTTGGCGCGAGCGCACTATACTGAATACAAAGATAGCATAATGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCt                          <  1:236909/150‑1 (MQ=255)
                                                                                                                          cTGACGGATGTGGCGCTAGAGCACTATCCGGCATACACAGATCGCATTAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCATAATAATTTATTTTATTTCAATAAGATAGATAATTAGAACTGAGAAAGCCATGCTCTCGTTTCCt                          <  2:881380/150‑1 (MQ=255)
                                                                                                                           tGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTAAAATCACCAAAATAAATTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTa                         <  2:672105/150‑1 (MQ=255)
                                                                                                                            gCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCGCAAATCACCAAAATAATTTATTTTATTTCATTAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCATaa                        >  1:861365/1‑150 (MQ=255)
                                                                                                                             ccGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAag                       <  1:615052/150‑1 (MQ=255)
                                                                                                                             ccGGATAGGGCGCTCGCGCCACATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAag                       >  1:1238563/1‑150 (MQ=255)
                                                                                                                               ggatagggCGCTCGCGCCACATCCGGCATACACAGATCGCATCAGGTTATGTTTAGTTTGTCATAAACCTCAAATCACCAAAAAAATTTATTTTATTTCAATAAGATAGATTAGTAGAACTGAGAAAGCGATGCTCTCGTTTCCTAagag                     >  2:988727/7‑150 (MQ=255)
                                                                                                                               ggATTTGGCGCGAGCGCCCTATCCGTCATACACAGATCTCATCAGGTTATTTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATATCAATAATATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAagag                     <  2:1570875/150‑1 (MQ=255)
                                                                                                                                   gtgGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAgttgt                 >  1:802691/1‑150 (MQ=255)
                                                                                                                                   gtgGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAgttgt                 <  2:851917/150‑1 (MQ=255)
                                                                                                                                     ggCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGttgttg               <  2:561914/150‑1 (MQ=255)
                                                                                                                                      gcgcGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGc              <  1:245217/150‑1 (MQ=255)
                                                                                                                                      gcgcGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATATTATTTCAAAAAGATAGATAAGTAGAACTGATAAACCCATCCTCTCGTTTCCTAAGAGTTGTTGc              >  2:184366/1‑150 (MQ=255)
                                                                                                                                       cgcgAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCCCCAAAATAATTTATTTTATTTAAATAAGATAGATAAGTAGAACTGAGAAAGCAATGATCTAGTTTCCTAAGAGTTGTTGCa             >  2:162828/1‑150 (MQ=255)
                                                                                                                                        gcgAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTAATTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCAt            >  2:699116/1‑150 (MQ=255)
                                                                                                                                             cgcCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGc       <  2:984663/150‑1 (MQ=255)
                                                                                                                                             cgcCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATGTTGc       >  2:1529095/1‑150 (MQ=255)
                                                                                                                                                ccTATCCGGAATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTAAAATCACCAAAATAATTTATATTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCtat    <  2:292590/150‑1 (MQ=255)
                                                                                                                                                 cTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCtata   <  1:511090/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GGCTGTACTGCTTTTAGCCTTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATAT  >  NC_000913/2041028‑2041323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: