Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 240,843 C→A 6.7% intergenic (+27/+16) ivy → / ← fadE inhibitor of c‑type lysozyme, periplasmic/acyl coenzyme A dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913240,8430CA 6.7% 315.1 / 8.5 112intergenic (+27/+16)ivy/fadEinhibitor of c‑type lysozyme, periplasmic/acyl coenzyme A dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (52/52);  new base A (5/3);  total (57/55)
Fisher's exact test for biased strand distribution p-value = 7.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.47e-01

Only 100 of 113 total aligned reads displayed.

CGCAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCAT  >  NC_000913/240695‑240992
                                                                                                                                                    |                                                                                                                                                     
gtcAAGAGAATCTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATTGTAAAACGTTGTTGTTCGCTGTGTTGACCGGCTGCCTGGAAAAGCATCCGGATGGCTTTAATTTTAAATATTTAGCGGATAAAGAAACGGTGCCTTTCg                                                                                                                                                      <  1:307527/148‑1 (MQ=255)
cGCAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCg                                                                                                                                                      >  2:1683763/1‑150 (MQ=255)
  cAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGc                                                                                                                                                    <  1:193418/150‑1 (MQ=255)
  cAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGc                                                                                                                                                    <  2:646163/150‑1 (MQ=255)
  cAAGAGAAACTCACCTGGCTGAATGTGAACGAAGCGCTTTCGATTGATGGTAAAACGGTCTAGTTCTCGGCCTTGACCGGCAGCCTGCAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGAGTATAAAGAAACGGATCCTTTCgag                                                                                                                                                    >  2:307527/1‑148 (MQ=255)
   aaGAGAAACTCACCTGGCTGAATGTGCACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCt                                                                                                                                                   >  1:246884/1‑150 (MQ=255)
       gaATCTCACCTGGCGGAATGTGAACGAAGCGCTATCGAGTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGt                                                                                                                                               <  2:309954/150‑1 (MQ=255)
       gaAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGt                                                                                                                                               >  1:896241/1‑150 (MQ=255)
       gaAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGt                                                                                                                                               <  2:1098919/150‑1 (MQ=255)
       gaAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGt                                                                                                                                               >  1:134616/1‑150 (MQ=255)
       gaAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGtt                                                                                                                                              <  2:1599091/150‑1 (MQ=255)
        aaaCTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGtt                                                                                                                                              <  2:519597/150‑1 (MQ=255)
        aaaCTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGtt                                                                                                                                              <  2:1185191/150‑1 (MQ=255)
          aCTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCGAAAGGCTCCGtttc                                                                                                                                            <  2:1241384/150‑3 (MQ=255)
           cTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTAtt                                                                                                                                           <  2:903515/150‑1 (MQ=255)
            tCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAACCGGAGCCGAAAGGCTCCGtttctt                                                                                                                                          <  2:813513/150‑5 (MQ=255)
            tCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCGAAAGGCTCCGtttctt                                                                                                                                          <  2:696226/150‑5 (MQ=255)
             cACCTGTCTGAATGTGAACGATGCGCTTTCGATTGATTGTAAAACTGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCa                                                                                                                                         <  2:1629755/150‑1 (MQ=255)
                           tgAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCa                                                                                                                           >  1:554951/1‑150 (MQ=255)
                            gAACGATGCGCTTCCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCaa                                                                                                                          >  2:322016/1‑150 (MQ=255)
                             aaCGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAACTAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAAc                                                                                                                         <  1:1053113/150‑1 (MQ=255)
                                           gATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTtct                                                                                                           >  1:1588420/1‑150 (MQ=255)
                                             ttGATGGTAATACGGTGTTGTTCGCGGCGGTGACCGGCAGCCTGGAAAACCATCCGGAGCGCTTTAATTTTAAATAATTTCCGGATAAAGAAACGGAGCCTTTCGGCGCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTcc                                                                                                         <  1:1339221/150‑1 (MQ=255)
                                                attgTAAAACGGTGTTGTTCGCGGCTTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGc                                                                                                      <  1:299454/147‑1 (MQ=255)
                                                aTGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGc                                                                                                      <  1:1683763/150‑1 (MQ=255)
                                                aTGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTACGCACTTTCTCCGGc                                                                                                      >  1:1709445/1‑150 (MQ=255)
                                                  ggTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGTCTTCAACTTTCCGCACTTTCTCAGGCaa                                                                                                    >  1:198606/1‑150 (MQ=255)
                                                   gTAAAACGGAGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAACCCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAAc                                                                                                   <  2:1088177/150‑1 (MQ=255)
                                                      aaaCGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAAAAATACGGATGGCTTAAATTTTAAATAATTAGCGGATAAAGAAACGGATCCTATCGGCTCAGTTATTCATTTACGCGGCTTCAACTTACCGCACTTTCTCCGGCAACttt                                                                                                >  1:498178/1‑150 (MQ=255)
                                                        aCGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAACGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTAc                                                                                              <  2:650369/150‑1 (MQ=255)
                                                        aCGGTGTTGTTCGCGGCGTAGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTAc                                                                                              <  2:635514/150‑1 (MQ=255)
                                                         ctgtgtTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTAcc                                                                                             <  2:1522733/148‑1 (MQ=255)
                                                         cGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTAcc                                                                                             <  1:989289/150‑1 (MQ=255)
                                                         cGGGGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCGTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTAcc                                                                                             <  1:1155054/150‑1 (MQ=255)
                                                          ggTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCg                                                                                            >  1:615310/1‑150 (MQ=255)
                                                              tttttCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCtt                                                                                        <  1:1187863/147‑1 (MQ=255)
                                                              ttgttCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCtt                                                                                        <  2:1313812/150‑1 (MQ=255)
                                                               tgttCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTtc                                                                                       >  1:28383/1‑150 (MQ=255)
                                                               tgttCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTAAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTtc                                                                                       >  1:1314144/1‑150 (MQ=255)
                                                               tgttCGCGGCGTTGACCGGCAGCCTGGAAAACCATACGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCTGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTTCCGGATtc                                                                                       >  2:1622359/1‑150 (MQ=255)
                                                                 ttCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTtcgt                                                                                     >  1:1654159/1‑150 (MQ=255)
                                                                  tCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGTATAATGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTtcgtc                                                                                    <  1:1410526/150‑1 (MQ=255)
                                                                  tCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTtcgtc                                                                                    >  1:1529483/1‑150 (MQ=255)
                                                                     gggcgTTGACCGGCAGCCTGGAAAACGAGCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGcc                                                                                 <  1:350837/149‑1 (MQ=255)
                                                                       gcgTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAg                                                                               <  1:617857/150‑1 (MQ=255)
                                                                        cgTTGACCGGCAGCCTGGTAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGc                                                                              >  2:1627306/1‑150 (MQ=255)
                                                                        cgTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGc                                                                              >  1:486492/1‑150 (MQ=255)
                                                                           tGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGctct                                                                           >  2:1024608/1‑150 (MQ=255)
                                                                             aCCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGAAAAAGAAACGGAGCCTTACGGCTCCGTTATTCATTTAAGCGGCTTCAACTTTACGCACTTTCACCGGCCACTATACCGGCTTCGTCGCCAGCTCTTc                                                                         >  2:264692/1‑150 (MQ=255)
                                                                                ggCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCATCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCgg                                                                      >  2:838600/1‑150 (MQ=255)
                                                                                 gCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGa                                                                     <  1:736993/150‑1 (MQ=255)
                                                                                      cTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCaaa                                                                >  1:1622388/1‑150 (MQ=255)
                                                                                      cTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCaaa                                                                >  1:433250/1‑150 (MQ=255)
                                                                                      cTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCaaa                                                                >  1:1054206/1‑150 (MQ=255)
                                                                                       tGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAg                                                               >  2:1033365/1‑150 (MQ=255)
                                                                                       tGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAg                                                               >  2:613480/1‑150 (MQ=255)
                                                                                       tGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAg                                                               <  1:1461317/150‑1 (MQ=255)
                                                                                       tGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTACGCACTTTATCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCaaat                                                               >  1:1273198/1‑149 (MQ=255)
                                                                                       tGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTAATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAg                                                               >  1:823499/1‑150 (MQ=255)
                                                                                             aCCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTTCCGGCTTCGTCGCCAGCTCTTCCGGAGCAAAGtgatca                                                         <  2:1594186/150‑1 (MQ=255)
                                                                                             aCCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGtcatca                                                         >  1:610486/1‑150 (MQ=255)
                                                                                                aTCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTTTTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACg                                                      >  1:1072906/1‑150 (MQ=255)
                                                                                                aTCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACg                                                      >  1:637102/1‑150 (MQ=255)
                                                                                                aTCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACg                                                      >  2:1527245/1‑150 (MQ=255)
                                                                                                  ccGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGtt                                                    >  2:1233293/1‑150 (MQ=255)
                                                                                                   cGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTa                                                   >  1:583095/1‑150 (MQ=255)
                                                                                                    ggATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTACGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCACTTCCGGATCAAAGTCATCAACGTTaa                                                  >  2:361630/1‑150 (MQ=255)
                                                                                                       tGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCTGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATAc                                               <  1:361651/150‑1 (MQ=255)
                                                                                                        ggCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACt                                              <  2:1588420/150‑1 (MQ=255)
                                                                                                         gCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATAATg                                             >  1:1435111/1‑150 (MQ=255)
                                                                                                         gCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACATTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGACATCAACGTTAATACTg                                             >  2:104395/1‑150 (MQ=255)
                                                                                                          cTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTgc                                            >  2:1323470/1‑150 (MQ=255)
                                                                                                          cTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTgc                                            <  2:610486/150‑1 (MQ=255)
                                                                                                           tttAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTgcg                                           <  2:246884/150‑1 (MQ=255)
                                                                                                           tttAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCGTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTgcg                                           <  1:927921/150‑1 (MQ=255)
                                                                                                            ttAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTgcgc                                          <  1:1089561/150‑1 (MQ=255)
                                                                                                               aTTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCAGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGa                                       >  2:43005/1‑150 (MQ=255)
                                                                                                                  ttAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACgg                                    >  2:922005/1‑150 (MQ=255)
                                                                                                                   tAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGc                                   <  1:428304/150‑1 (MQ=255)
                                                                                                                     aataatTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCtt                                 >  1:1706923/1‑150 (MQ=255)
                                                                                                                        aatTAGCGGATAAAGAAACGGCGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTttct                              <  2:1057491/150‑1 (MQ=255)
                                                                                                                        aatTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTGCGGATCAAAGTCATCAACGTTAATTCTGCGCAGACGGCTttct                              >  2:891436/1‑150 (MQ=255)
                                                                                                                        aatTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTttct                              <  1:632434/150‑1 (MQ=255)
                                                                                                                            aGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAg                          <  1:1348771/150‑1 (MQ=255)
                                                                                                                            aGCGGATAAAGAAACGGAGCCATTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAg                          <  1:394183/150‑1 (MQ=255)
                                                                                                                               ggATAACGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCtt                       <  1:104691/150‑1 (MQ=255)
                                                                                                                                gATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCttt                      <  2:134616/150‑1 (MQ=255)
                                                                                                                                gATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCttt                      <  2:896241/150‑1 (MQ=255)
                                                                                                                                gATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGAACAAAGACATCAACGTTAATACTGCGCTGACGGCTTTATTCAGAttt                      >  2:288429/1‑150 (MQ=255)
                                                                                                                                   tgaataaCGGAGCCGAAAGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCAc                   >  1:800571/6‑150 (MQ=255)
                                                                                                                                   tgaataaCGGAGCCGAAAGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCAc                   >  1:795167/6‑150 (MQ=255)
                                                                                                                                     aGAAACGGAGCCTTTCGCCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTATACCGGCTTCGTCGCCATCTCTTCCGGATCAAAGTCATCAACGATAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCa                 <  1:204651/150‑1 (MQ=255)
                                                                                                                                        aaCGGAGCCGAAAGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCAACAGaa              >  2:1329798/1‑150 (MQ=255)
                                                                                                                                        aaCGGAGCCGAAAGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTATCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCAGATCAAAGTCATCAACGTTAATAATGCGCAGACGGCTTTCTTCCGCTTTCACCAGaa              >  1:1088727/1‑150 (MQ=255)
                                                                                                                                         aCGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTTCCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAAt             <  2:554951/150‑1 (MQ=255)
                                                                                                                                             aGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCg         <  1:1176614/150‑1 (MQ=255)
                                                                                                                                                cTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCt      <  1:718078/150‑1 (MQ=255)
                                                                                                                                                cTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCt      >  2:1203379/1‑150 (MQ=255)
                                                                                                                                                   tCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCa   <  2:9686/150‑1 (MQ=255)
                                                                                                                                                    cGGCTCCGTTATTCATTTACGCGGCATCAACTTTCCGCACTTTCTCCGGCATATTTACCGGCTACGACCCCAGCTCTTCCCGATCAAAGTCATAAACGTTAATAATCAGCAGACGGCTTTCTTCAGCTTTCGCCACCATAGAGGcatcat  >  2:1152939/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGCAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCAT  >  NC_000913/240695‑240992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: