Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,057,597 T→G 7.8% intergenic (+23/+430) yeeN → / ← asnW UPF0082 family protein/tRNA‑Asn

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,057,5970TG 7.8% 423.7 / 20.9 153intergenic (+23/+430)yeeN/asnWUPF0082 family protein/tRNA‑Asn
Reads supporting (aligned to +/- strand):  ref base T (71/68);  new base G (7/5);  total (80/73)
Fisher's exact test for biased strand distribution p-value = 7.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-01

Only 100 of 155 total aligned reads displayed.

AGAAATGATTGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCA  >  NC_000913/2057448‑2057745
                                                                                                                                                     |                                                                                                                                                    
aGAAATGATTGATCAATCTGAAGTTGAGCTTTCCCCATAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGAAAATCTCTAATTATCTTTTAAAGAAATCTGTCt                                                                                                                                                      <  2:1663292/150‑1 (MQ=255)
     tGATTGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACg                                                                                                                                                 >  2:1225652/1‑150 (MQ=255)
      gATTGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACgg                                                                                                                                                >  1:1626072/1‑150 (MQ=255)
       aTTGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGc                                                                                                                                               <  2:682210/150‑1 (MQ=255)
         tGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAg                                                                                                                                             >  1:432/1‑150 (MQ=255)
          gCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGa                                                                                                                                            >  2:586028/1‑150 (MQ=255)
            tCATTCTGAAGTTGAGCTTTCACCATAAGTTTTAGAAATCTTTGAATGGCTTGTTGATGCCATTGAAGATGACGACGATTTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGAtt                                                                                                                                          <  1:331715/150‑1 (MQ=255)
            tCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGAtt                                                                                                                                          <  2:505641/150‑1 (MQ=255)
            tCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCATTTAAAGAAATCTGTCTTTACGGCAGAtt                                                                                                                                          <  1:604096/150‑1 (MQ=255)
              aaTCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGAttta                                                                                                                                        >  1:1660274/1‑149 (MQ=255)
              aaTCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGAttta                                                                                                                                        >  1:119773/1‑149 (MQ=255)
               aTCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGATGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCt                                                                                                                                       >  2:1715101/1‑150 (MQ=255)
               aTCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCt                                                                                                                                       >  2:738608/1‑150 (MQ=255)
                tCTGACGTTGAGCTCTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCAAAACGTCGCAAATCTCTAATTATCTTTAAAAGAAATCTGTCTTTACGGCAGATTTCtt                                                                                                                                      <  1:275840/150‑1 (MQ=255)
                tCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCtt                                                                                                                                      <  2:751086/150‑1 (MQ=255)
                 cTGCAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCttt                                                                                                                                     <  1:750360/150‑1 (MQ=255)
                 cTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCttt                                                                                                                                     >  1:1692098/1‑150 (MQ=255)
                 cTGAAGTTGAGCTTTCCCCACAAGCTTTAGAAATCTTTGAAGGGCTTGATGATGCCCTTGAAGATGACGTCCATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCttt                                                                                                                                     <  1:837857/150‑1 (MQ=255)
                 atGAAGTTGAGCTTTCCCCATATGATTTATAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGAAGACGATGTACAAAAAGTTTATCATAACGTAGCATATCTCTAATTATCTTTTTAAGAAATCTGTCTTTACGGCAGATTTCttt                                                                                                                                     <  1:724407/149‑1 (MQ=255)
                    aaGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAAt                                                                                                                                  <  1:1622739/150‑1 (MQ=255)
                     aGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGCCGTAAAGACAGATTTCTTTaaaa                                                                                                                                 <  2:53354/150‑3 (MQ=39)
                      gTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAAtct                                                                                                                                >  1:1722113/1‑150 (MQ=255)
                        tGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCa                                                                                                                              >  1:396104/1‑150 (MQ=255)
                         gAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCat                                                                                                                             <  2:101109/150‑1 (MQ=255)
                           gCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCatat                                                                                                                           <  2:611075/150‑1 (MQ=255)
                             tttCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATaa                                                                                                                         >  1:288634/1‑150 (MQ=255)
                               tCCCCAGAAGATTTAGAAATCTTTGAAGTGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAAtt                                                                                                                       <  1:1212827/150‑1 (MQ=255)
                               tCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAAtt                                                                                                                       <  1:1291994/150‑1 (MQ=255)
                                  ccAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCtt                                                                                                                    <  2:1487432/150‑1 (MQ=255)
                                  ccAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCtt                                                                                                                    >  1:574345/1‑150 (MQ=255)
                                    agaagaTTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTtat                                                                                                                  <  2:282248/150‑1 (MQ=255)
                                    agaagaTTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTtat                                                                                                                  >  1:103931/1‑150 (MQ=255)
                                     gaagaTTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTtata                                                                                                                 <  1:566463/150‑1 (MQ=255)
                                        gaTTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATaaaa                                                                                                              >  2:1284037/1‑150 (MQ=255)
                                              gAAATCTTTGAAGGGCTTGTTGATGCCCTTTAAGAAGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCATAACCGCAAAAAACTATAATCTCATATAAAACAAATAAAAaaaata                                                                                                        >  1:1014028/1‑150 (MQ=255)
                                               aaaTCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatataa                                                                                                       >  2:562269/1‑150 (MQ=255)
                                                aaTCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAaatataat                                                                                                      <  1:739342/150‑1 (MQ=255)
                                                   cTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATAtt                                                                                                   >  1:876095/1‑150 (MQ=255)
                                                    tttGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTc                                                                                                  >  1:1170141/1‑150 (MQ=255)
                                                     ttGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCa                                                                                                 <  2:1692098/150‑1 (MQ=255)
                                                       gAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCTAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCaaa                                                                                               >  2:1221365/1‑149 (MQ=255)
                                                       gAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAAc                                                                                               >  2:212286/1‑150 (MQ=255)
                                                       gAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAAc                                                                                               <  1:1198185/150‑1 (MQ=255)
                                                         aGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTc                                                                                             >  2:1444213/1‑150 (MQ=255)
                                                          gggCTTGTTGATGGCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAATTCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAAGTCg                                                                                            <  2:1589545/150‑1 (MQ=255)
                                                           ggCTTGTTGCTGCACTTGAAGATGACGACGACGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTTTCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATATAAAATATAATATTCAACTAGt                                                                                           >  2:1129476/1‑150 (MQ=255)
                                                            gCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTc                                                                                          >  1:831490/1‑150 (MQ=255)
                                                            gCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTATACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTc                                                                                          >  1:19849/1‑150 (MQ=255)
                                                             gttgttgATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCa                                                                                         <  2:672385/149‑1 (MQ=255)
                                                              ttgttgATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCat                                                                                        <  2:1356678/150‑1 (MQ=255)
                                                                gttgATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCatat                                                                                      <  2:975256/150‑1 (MQ=255)
                                                                gttgATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCatat                                                                                      <  1:1693848/150‑1 (MQ=255)
                                                                 ttgATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATAtt                                                                                     <  2:409403/150‑1 (MQ=255)
                                                                  tgATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATAttt                                                                                    >  2:458600/1‑149 (MQ=255)
                                                                     tGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAAAGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGAAGATTTCTTTAATCTAATATAATTCTTATAAAAAATATAATATTCAACTCGTCATTTTGAtt                                                                                 >  2:436737/1‑150 (MQ=255)
                                                                       cccTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATtat                                                                               >  1:507890/1‑150 (MQ=255)
                                                                           tGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAAATCGTCATATTGATTATAccc                                                                           >  1:994275/1‑150 (MQ=255)
                                                                                       cgaTGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCACag                                                               >  2:1673582/1‑150 (MQ=255)
                                                                                       agaTGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTTTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCag                                                               <  1:80805/149‑1 (MQ=255)
                                                                                        gaTGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCaga                                                              <  2:1732669/150‑1 (MQ=255)
                                                                                        gaTGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATACTGATTATACCCCCCCGTTCCCaga                                                              <  2:1302361/150‑1 (MQ=255)
                                                                                                aaaaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAaataat                                                      <  2:288634/150‑1 (MQ=255)
                                                                                                aaaaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAaataat                                                      >  2:770029/1‑150 (MQ=255)
                                                                                                aaaaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAaataat                                                      <  1:1725306/150‑1 (MQ=255)
                                                                                                  aaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAatat                                                    >  2:366511/1‑150 (MQ=255)
                                                                                                   aGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAAtatt                                                   <  2:876095/150‑1 (MQ=255)
                                                                                                   aGTTTATCAAAACGTCGCAAATCTCATATTATCTTTTAAAGAAATCTGTCTTTACTGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAAtatt                                                   <  2:335495/150‑1 (MQ=255)
                                                                                                    gTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAAtattt                                                  >  2:659301/1‑150 (MQ=255)
                                                                                                      ttATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAAtatttat                                                >  2:1542273/1‑150 (MQ=255)
                                                                                                       tATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAAtatttatt                                               >  2:619816/1‑150 (MQ=255)
                                                                                                            tAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTAATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCACAGTTCCCAGAGAATTTATATTTATTAAAAt                                          >  2:80964/1‑150 (MQ=255)
                                                                                                                gTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCGGAGAAATAATATTTATTAAAATTCCa                                      <  1:1575497/150‑1 (MQ=255)
                                                                                                                gTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCa                                      >  1:112993/1‑150 (MQ=255)
                                                                                                                gTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCACCCGTTCCCAGAGAAATAATATTTATTAAAATTCCa                                      >  1:1335246/1‑150 (MQ=255)
                                                                                                                    cAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGTCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATATTATTTATTAAAATTCCAGttc                                  <  2:58769/150‑1 (MQ=255)
                                                                                                                    cAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACACCCCCGTTACCAGAGAAATAATATTTATTAAAATTCAAGtaa                                  >  1:1474114/1‑148 (MQ=255)
                                                                                                                    cAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACACCCCAGTTACCAGAGAAATAATATTTATTAAAATTCAAGtta                                  >  1:1467227/1‑149 (MQ=255)
                                                                                                                            tAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCttttt                          <  2:114316/150‑1 (MQ=255)
                                                                                                                              aTTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCt                        <  2:1690687/150‑1 (MQ=255)
                                                                                                                              aTTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCt                        <  1:212286/150‑1 (MQ=255)
                                                                                                                              aTTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAAATCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCt                        <  1:436737/150‑1 (MQ=255)
                                                                                                                                 aTCTTTTAAAGAAATCGGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTTATTATACCACCCCGTTCGCAGAGAAATAATATTTATTAAAATTCGAGTGCTACTTTTTCTGAt                     >  1:680105/1‑150 (MQ=255)
                                                                                                                                  tCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGAtt                    <  2:1527935/150‑1 (MQ=255)
                                                                                                                                    ttttAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTAc                  <  1:726950/150‑1 (MQ=255)
                                                                                                                                     tttAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCTTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTACAGTTCTTCTTTTTATGATTACa                 >  2:1477457/1‑150 (MQ=255)
                                                                                                                                        aaaGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGaa              >  2:1378464/1‑150 (MQ=255)
                                                                                                                                        aaaGAAATCTGCCGTAAAGACAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGaa              >  2:1614713/1‑150 (MQ=255)
                                                                                                                                        aaaGAAATCTGCCGTAAAGACAGATTTCATTAATCTCATATAATACTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACACCACCGTTCACAGAGAAATAATATTTATTAAAATTCCAGTTCTTATTTTTATCATTACAGaa              >  1:323272/1‑150 (MQ=255)
                                                                                                                                        aaaGAAATCTGCCGTAAAGACAGATTACTTTAATCTCATATAATTCTTATAAAAAATATAATATACAACTCTACATATTGATTATACACCACCGTAACCAGAGAAATAATATTTATTAAAATAACAGTTCTTCTATTACTGATTACAGaa              >  1:1031646/1‑150 (MQ=255)
                                                                                                                                         aaGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAg             <  2:324319/150‑1 (MQ=255)
                                                                                                                                         aaGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAg             >  1:1360702/1‑150 (MQ=255)
                                                                                                                                         aaGAAATCTGTCTTTACGGAAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGATAAATATTATTTATTAAAATTACAGTTGTTGTTGTTCTGATTAaagaag             >  1:266795/1‑150 (MQ=255)
                                                                                                                                         aaGAAATCTGCCGTAAAGACAGATTTCTTTAA‑‑‑AAGATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTGTTTTTCTGATTACAGAAGGCa          >  2:496085/1‑150 (MQ=37)
                                                                                                                                           gAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGc           >  2:390328/1‑150 (MQ=255)
                                                                                                                                           gAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGc           >  1:391454/1‑150 (MQ=255)
                                                                                                                                             aaTCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCaa         >  2:781343/1‑150 (MQ=255)
                                                                                                                                                cTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTAAAGAAGGCAAAGt      >  1:1282895/1‑150 (MQ=255)
                                                                                                                                                  gTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTgg    <  1:1554561/150‑1 (MQ=255)
                                                                                                                                                   tCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGc   <  2:103931/150‑1 (MQ=255)
                                                                                                                                                    cTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCAAATTGAATATACCACCCAGTTACCAGAGAAAAAATATTTATTAAAATTCCAGTTATTCTATTACTTATTAAATAAGAAAAAGTgtaa  >  1:1064453/1‑147 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AGAAATGATTGCTCAATCTGAAGTTGAGCTTTCCCCAGAAGATTTAGAAATCTTTGAAGGGCTTGTTGATGCCCTTGAAGATGACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCA  >  NC_000913/2057448‑2057745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: