Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,068,407 A→G 9.4% pseudogene (13/159 nt) yoeH ← pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,068,4070AG 9.4% 314.8 / 20.2 119pseudogene (13/159 nt)yoeHpseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base A (64/43);  new base G (4/7);  total (69/50)
Fisher's exact test for biased strand distribution p-value = 2.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.63e-01

Only 100 of 121 total aligned reads displayed.

GGACCTGATACTGATATGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACAAT  >  NC_000913/2068258‑2068554
                                                                                                                                                     |                                                                                                                                                   
ggACCTGATACTGATATGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTa                                                                                                                                                     <  1:1208670/150‑1 (MQ=255)
ggACCTGATACTGATATGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTa                                                                                                                                                     >  2:1104120/1‑150 (MQ=255)
 gACCTGATACTGATATGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTAt                                                                                                                                                    >  2:540479/1‑150 (MQ=255)
 gACCTGATACTGATATGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTAt                                                                                                                                                    >  1:782432/1‑150 (MQ=255)
   ccTGATACTGATATGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGt                                                                                                                                                  <  1:1281/150‑1 (MQ=255)
      gatactgataTGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCaa                                                                                                                                               >  2:1435112/1‑150 (MQ=255)
            gataTGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGTTACCTTCTATCAGACACCATTTTTTCAACTCAGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGc                                                                                                                                         >  1:97320/1‑150 (MQ=255)
            gataTGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACTGACCGCACTCTTATGTCAAGAGTGc                                                                                                                                         >  1:1723394/1‑150 (MQ=255)
             atatGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCg                                                                                                                                        >  2:327070/1‑150 (MQ=255)
              tatGAGTCTTACCTCATATACGGTCAATTTCAGAAATTACTTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTGACATAAGAGTGCgg                                                                                                                                       <  2:120401/150‑1 (MQ=255)
              tatGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTGACATAAGAGTGCgg                                                                                                                                       <  1:802229/150‑1 (MQ=255)
              tatGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTGACATAAGAGTGCgg                                                                                                                                       <  1:633752/150‑1 (MQ=255)
              tatGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTGACATAAGAGTGCgg                                                                                                                                       <  1:561024/150‑1 (MQ=255)
               atGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTGACATAAGAGTGCGGt                                                                                                                                      <  1:1355367/150‑1 (MQ=255)
                tGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTc                                                                                                                                     <  2:1632991/150‑1 (MQ=255)
                tGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATAATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTc                                                                                                                                     >  2:703487/1‑150 (MQ=255)
                 gAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCATTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTcc                                                                                                                                    >  2:1194827/1‑150 (MQ=255)
                    tCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTAGACATAAGAGTGCGGTCCGTg                                                                                                                                 <  1:1489797/150‑1 (MQ=39)
                     cTTACCGCATATACGGTCAATTTCAGCAATTAATAACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCAATTTTACAAATCCGTTTTCCACGGACCGCACTCTAATGTCAAGAGTGCGGTCCGTgg                                                                                                                                >  1:1132158/1‑150 (MQ=255)
                        aCCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGCCCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATa                                                                                                                             >  2:942227/1‑150 (MQ=255)
                           gCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCTAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACaa                                                                                                                          >  1:288813/1‑150 (MQ=255)
                           gCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGAACGCACTCTTATGTAAAGAGTGCGGTCCGTGGATACaa                                                                                                                          >  1:981990/1‑150 (MQ=255)
                              tataCGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCa                                                                                                                       <  2:1237148/150‑1 (MQ=255)
                              tataCGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGAAACCATTTTTTCAACTCAGTTTTCCACGGACCGCAATATTATTTAAAGAGTTCCGTACGTGGAaacaacaa                                                                                                                       >  1:603022/1‑148 (MQ=255)
                               ataCGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCag                                                                                                                      >  1:227798/1‑150 (MQ=255)
                               ataCGGTCAATTTCAGCAATTAATTACATTAACCACGCCAAAGTATTTGTCATCACAATGATGTTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCag                                                                                                                      <  1:1524220/150‑1 (MQ=255)
                                 aCGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTAAAGAGTGCGGTCCGTGGATACAACCagag                                                                                                                    >  2:596657/1‑150 (MQ=255)
                                   ggTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGAc                                                                                                                  <  2:457514/150‑1 (MQ=255)
                                   ggTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACAGCACTCTTATGTCAAGAGTCCGGTCCCTGGAAACAACCAGAAAc                                                                                                                  >  1:478125/1‑150 (MQ=255)
                                    gTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTAATCACAATGATGGTACCTTCTTTAATACACCATTTTTTAAACTAAGTATTCAACGGACAGCACTATTAAGACAAGAGTGATCTCCGTGTATACAACCagataac                                                                                                                 >  1:1216507/1‑146 (MQ=255)
                                       aaTTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTACGTGGATACAACCAGAGACCGaa                                                                                                              >  1:1372579/1‑149 (MQ=255)
                                        aTTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACt                                                                                                             <  2:278442/150‑1 (MQ=255)
                                         tttCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTg                                                                                                            <  2:756802/150‑1 (MQ=255)
                                         tttCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTg                                                                                                            <  1:913779/150‑1 (MQ=255)
                                            cAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGaca                                                                                                         <  1:942227/150‑1 (MQ=255)
                                              gCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGacacc                                                                                                       >  1:624283/1‑149 (MQ=255)
                                              gCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACg                                                                                                       >  1:55259/1‑150 (MQ=255)
                                              gCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACACTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACg                                                                                                       >  1:1002756/1‑150 (MQ=255)
                                               cAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGa                                                                                                      >  1:1441753/1‑150 (MQ=255)
                                                aattaattACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACACTTATGTCAAGAGTGCGGTCCGTGGATTCAACCAGAGGCCCACTCACACGaa                                                                                                     >  1:592031/1‑149 (MQ=255)
                                                  ttaattaCATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTc                                                                                                   <  1:1722493/150‑1 (MQ=255)
                                                     attaCATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCaga                                                                                                <  2:1354622/150‑1 (MQ=255)
                                                     attaCATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACGCGGATGGCAAGACTGCGGTGCGTGGTTACAACCAGATACCGGCAGCCCCGAGTCaga                                                                                                >  2:1497724/1‑150 (MQ=255)
                                                        aCATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGa                                                                                             >  2:668017/1‑150 (MQ=255)
                                                                 aCGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTAAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGa                                                                                    >  1:1113478/1‑150 (MQ=255)
                                                                  cGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTACGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAAAGACGGat                                                                                   >  2:195120/1‑150 (MQ=255)
                                                                   gCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGata                                                                                  >  1:1189087/1‑150 (MQ=255)
                                                                     cAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATg                                                                                >  1:358104/1‑150 (MQ=255)
                                                                      aaaGTATTTGTCATCACAATGATGGTACCTCCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGt                                                                               >  2:768198/1‑150 (MQ=255)
                                                                        aGTATTTGTCATCACAATGATGGTTCCTTTTTTCAGACACCATTTTTTCAACTCCGATTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTc                                                                             <  2:1688265/150‑1 (MQ=255)
                                                                          tATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCGTATGTCAAGAGTGCGGTCCGTGGCTAAAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAg                                                                           >  1:1362067/1‑150 (MQ=255)
                                                                           aTTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGt                                                                          <  2:1442710/150‑1 (MQ=255)
                                                                           aTTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGt                                                                          >  1:1392990/1‑150 (MQ=255)
                                                                              tGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCCCGCTTATGTCAAGCGTGCGGTCCGTGGATTCAACCCGAGACCGCCTGCCACGAGTCAGAGGAAACGACGGATATGTTCAGTCgg                                                                       >  1:1398604/1‑149 (MQ=255)
                                                                              tGTCATCACAATGATGGTACCTTATTTCAGACACCATTTTTTCAACTCCGCTTTCCACGGACCGCACTCTTATGTCAAGAGGGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGt                                                                       <  2:760446/150‑1 (MQ=255)
                                                                               gTCAGCACACTGATGGTACCTTCTTTCAGACACCATTTTTTCACCTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTa                                                                      <  1:116116/150‑1 (MQ=255)
                                                                                 catcaCAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTaaa                                                                    >  2:615616/1‑150 (MQ=255)
                                                                                 catcaCAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTaaa                                                                    >  2:895330/1‑150 (MQ=255)
                                                                                      caATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTATTATTTAAAGATTGCGGTCAGTGGCTACAACCAGAGACCGACTTAAACGAGTCAGAGGAAACGACGCAAATGTTCAGTCGTAAAatata                                                               >  2:572192/1‑149 (MQ=255)
                                                                                       aaTGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACTGACCGCACTCTTATGTCAAGAGTGCGGTCCGAGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAAtatct                                                              <  1:327070/150‑1 (MQ=255)
                                                                                       aaTGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAAtatct                                                              <  2:785131/150‑1 (MQ=255)
                                                                                        atgatgGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAAtatcta                                                             >  2:130867/1‑150 (MQ=255)
                                                                                           atgGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCa                                                          <  2:403685/150‑1 (MQ=255)
                                                                                            tgGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTTTGTCAAGAGTGCGGGCCGTGGATACCACCAGAGACCGACAGACACGAGTCAGGGGAAACGACGGATAAGTTCAGTCGGAAAATATATATCaa                                                         >  2:1095798/1‑150 (MQ=255)
                                                                                                 ccTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAAc                                                    <  1:1139920/150‑1 (MQ=255)
                                                                                                     cttTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGa                                                >  1:312119/1‑150 (MQ=255)
                                                                                                        tCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGCCACGAGTCAGAGGAAACGCCGGATATGTTCAATAGTAAAATATCAATCAAAAAACATGATTa                                             >  1:861899/1‑150 (MQ=255)
                                                                                                          aGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGCCCGACTGACACGAGTCAGAGGAAAAGACGGATATTTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAg                                           >  1:1703242/1‑150 (MQ=255)
                                                                                                           gACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAgg                                          <  2:1098258/150‑1 (MQ=255)
                                                                                                           gACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAgg                                          >  1:1048035/1‑150 (MQ=255)
                                                                                                           gACACCATTTTTTCAACTCCGTTATCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAgg                                          <  1:1435112/150‑1 (MQ=255)
                                                                                                            acacCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGt                                         <  2:546053/150‑1 (MQ=255)
                                                                                                             cagcATTTTTTCAACTCCGTTTTCCACTGACCGCACTCTTATGTCAAGAGTGCGGTCTGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTc                                        <  1:962643/147‑1 (MQ=255)
                                                                                                             cacCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTc                                        >  2:585566/1‑150 (MQ=255)
                                                                                                             cacCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTAAGTCGTAAAATATCTATCAAAAAACATGATTAATGTc                                        >  2:325425/1‑150 (MQ=255)
                                                                                                              acCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGCCCGACTGCCACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCa                                       >  1:1382921/1‑150 (MQ=255)
                                                                                                               ccATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCaa                                      >  1:955957/1‑150 (MQ=255)
                                                                                                                   tttttCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAAt                                  <  1:540479/150‑1 (MQ=255)
                                                                                                                               tttttCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGAAAGACACGAGTCAGAGGAAACGACGGATATGTTCAGTATTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTa                      <  1:1124781/149‑1 (MQ=255)
                                                                                                                                ttttCCACTGACCGAACTCTTATGTCAAGAGTGCGGTACGTGGATACAACCAGAGACCTACTGACACGAGTCAGAGTAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGGCAAAAATGTTTGATATTTAc                     <  1:273664/150‑1 (MQ=255)
                                                                                                                                ttttCCACGGACCGCGCTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTAc                     >  1:898742/1‑150 (MQ=255)
                                                                                                                                ttttCCACGGACCGCACTCTTATGTCAATAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCATAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTAc                     <  2:1534298/150‑1 (MQ=255)
                                                                                                                                ttttCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTAc                     <  2:1716620/150‑1 (MQ=255)
                                                                                                                                    ccACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAAtt                 <  2:601988/150‑1 (MQ=255)
                                                                                                                                    ccACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGATTGATATTTAAAAtt                 >  2:277800/1‑150 (MQ=255)
                                                                                                                                     cACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAAttt                >  2:932839/1‑150 (MQ=255)
                                                                                                                                       cGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTAt              <  1:1095798/150‑1 (MQ=255)
                                                                                                                                        ggACCGCACTCTTGACATAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGCCACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACATTTTATg             >  2:1023580/1‑150 (MQ=39)
                                                                                                                                          aCCGCACTCTTGACATAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGaa           >  1:1468504/1‑150 (MQ=255)
                                                                                                                                          aCCGCACTCTTGACATAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGaa           >  1:1034194/1‑150 (MQ=255)
                                                                                                                                           ccGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAg          <  1:1104120/150‑1 (MQ=255)
                                                                                                                                             gCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGac        >  2:488417/1‑149 (MQ=255)
                                                                                                                                             gCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGAt        <  1:1210464/150‑1 (MQ=255)
                                                                                                                                             gCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGAt        >  1:1274355/1‑150 (MQ=255)
                                                                                                                                             gCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGAt        >  2:1553235/1‑150 (MQ=255)
                                                                                                                                             gCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGAt        >  1:1030207/1‑150 (MQ=255)
                                                                                                                                             gCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGATTGATATTTACAATTTATGAAGAt        >  2:1516090/1‑150 (MQ=255)
                                                                                                                                             gCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGACAAAAATGTTTGATATTTAAAATTTATGAAGAt        >  1:1274364/1‑150 (MQ=255)
                                                                                                                                                 tctTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACa    <  1:596657/150‑1 (MQ=255)
                                                                                                                                                   ttATGTCAAGAGTGCGGTGCGTGGATACAACCAGAGACCGAATGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACaat  <  1:1643527/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GGACCTGATACTGATATGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACAAT  >  NC_000913/2068258‑2068554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: