Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,068,408 T→A 9.7% pseudogene (12/159 nt) yoeH ← pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,068,4080TA 9.7% 291.9 / 15.8 117pseudogene (12/159 nt)yoeHpseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base T (62/43);  new base A (5/7);  total (67/50)
Fisher's exact test for biased strand distribution p-value = 3.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 119 total aligned reads displayed.

GACCTGATACTGATATGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACAAT  >  NC_000913/2068259‑2068554
                                                                                                                                                     |                                                                                                                                                  
gACCTGATACTGATATGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTAt                                                                                                                                                    >  1:782432/1‑150 (MQ=255)
gACCTGATACTGATATGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTAt                                                                                                                                                    >  2:540479/1‑150 (MQ=255)
  ccTGATACTGATATGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGt                                                                                                                                                  <  1:1281/150‑1 (MQ=255)
     gatactgataTGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCaa                                                                                                                                               >  2:1435112/1‑150 (MQ=255)
           gataTGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGTTACCTTCTATCAGACACCATTTTTTCAACTCAGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGc                                                                                                                                         >  1:97320/1‑150 (MQ=255)
            atatGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCg                                                                                                                                        >  2:327070/1‑150 (MQ=255)
             tatGAGTCTTACCTCATATACGGTCAATTTCAGAAATTACTTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTGACATAAGAGTGCgg                                                                                                                                       <  2:120401/150‑1 (MQ=255)
             tatGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTGACATAAGAGTGCgg                                                                                                                                       <  1:802229/150‑1 (MQ=255)
             tatGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTGACATAAGAGTGCgg                                                                                                                                       <  1:633752/150‑1 (MQ=255)
             tatGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTGACATAAGAGTGCgg                                                                                                                                       <  1:561024/150‑1 (MQ=255)
              atGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTGACATAAGAGTGCGGt                                                                                                                                      <  1:1355367/150‑1 (MQ=255)
               tGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTc                                                                                                                                     <  2:1632991/150‑1 (MQ=255)
               tGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTGCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTGACATAAGAGTGCGGTc                                                                                                                                     <  1:1242375/150‑1 (MQ=255)
               tGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATAATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTc                                                                                                                                     >  2:703487/1‑150 (MQ=255)
                gAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCATTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTcc                                                                                                                                    >  2:1194827/1‑150 (MQ=255)
                    cTTACCGCATATACGGTCAATTTCAGCAATTAATAACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCAATTTTACAAATCCGTTTTCCACGGACCGCACTCTAATGTCAAGAGTGCGGTCCGTgg                                                                                                                                >  1:1132158/1‑150 (MQ=255)
                       aCCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGCCCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATa                                                                                                                             >  2:942227/1‑150 (MQ=255)
                         cGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACa                                                                                                                           <  2:561640/150‑1 (MQ=255)
                          gCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCTAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACaa                                                                                                                          >  1:288813/1‑150 (MQ=255)
                          gCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGAACGCACTCTTATGTAAAGAGTGCGGTCCGTGGATACaa                                                                                                                          >  1:981990/1‑150 (MQ=255)
                             tataCGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGAAACCATTTTTTCAACTCAGTTTTCCACGGACCGCAATATTATTTAAAGAGTTCCGTACGTGGAaacaacaa                                                                                                                       >  1:603022/1‑148 (MQ=255)
                              ataCGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCag                                                                                                                      >  1:227798/1‑150 (MQ=255)
                              ataCGGTCAATTTCAGCAATTAATTACATTAACCACGCCAAAGTATTTGTCATCACAATGATGTTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCag                                                                                                                      <  1:1524220/150‑1 (MQ=255)
                                aCGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTAAAGAGTGCGGTCCGTGGATACAACCagag                                                                                                                    >  2:596657/1‑150 (MQ=255)
                                aCGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACACTTATGTCAAGAGTGCGGTCCGTGGATAAAACCagac                                                                                                                    >  1:1366119/1‑149 (MQ=255)
                                  ggTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGAc                                                                                                                  <  2:457514/150‑1 (MQ=255)
                                  ggTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACAGCACTCTTATGTCAAGAGTCCGGTCCCTGGAAACAACCAGAAAc                                                                                                                  >  1:478125/1‑150 (MQ=255)
                                   gTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTAATCACAATGATGGTACCTTCTTTAATACACCATTTTTTAAACTAAGTATTCAACGGACAGCACTATTAAGACAAGAGTGATCTCCGTGTATACAACCagataac                                                                                                                 >  1:1216507/1‑146 (MQ=255)
                                      aaTTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTACGTGGATACAACCAGAGACCGaa                                                                                                              >  1:1372579/1‑149 (MQ=255)
                                       aTTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATTATGGTACCTTCTTTCAGAAACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACt                                                                                                             <  1:1563861/150‑1 (MQ=255)
                                       aTTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACt                                                                                                             <  2:278442/150‑1 (MQ=255)
                                        tttCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTg                                                                                                            <  2:756802/150‑1 (MQ=255)
                                        tttCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTg                                                                                                            <  1:913779/150‑1 (MQ=255)
                                           cAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGaca                                                                                                         <  1:942227/150‑1 (MQ=255)
                                            aGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACCGACCGCACTCTTATGTAAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGacac                                                                                                        >  1:367275/1‑150 (MQ=255)
                                             gCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGacacc                                                                                                       >  1:624283/1‑149 (MQ=255)
                                             gCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACg                                                                                                       >  1:55259/1‑150 (MQ=255)
                                             gCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACg                                                                                                       >  1:1098258/1‑150 (MQ=255)
                                             gCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACACTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACg                                                                                                       >  1:1002756/1‑150 (MQ=255)
                                              cAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGa                                                                                                      >  1:1441753/1‑150 (MQ=255)
                                               aattaattACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACACTTATGTCAAGAGTGCGGTCCGTGGATTCAACCAGAGGCCCACTCACACGaa                                                                                                     >  1:592031/1‑149 (MQ=255)
                                                 ttaattaCATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTc                                                                                                   <  1:1722493/150‑1 (MQ=255)
                                                    attaCATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCaga                                                                                                <  2:1354622/150‑1 (MQ=255)
                                                       aCATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGa                                                                                             >  2:668017/1‑150 (MQ=255)
                                                                aCGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTAAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGa                                                                                    >  1:1113478/1‑150 (MQ=255)
                                                                 cGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTACGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAAAGACGGat                                                                                   >  2:195120/1‑150 (MQ=255)
                                                                  gCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGata                                                                                  >  1:1189087/1‑150 (MQ=255)
                                                                    cAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATg                                                                                >  1:358104/1‑150 (MQ=255)
                                                                         tATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCGTATGTCAAGAGTGCGGTCCGTGGCTAAAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAg                                                                           >  1:1362067/1‑150 (MQ=255)
                                                                          aTTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGt                                                                          >  2:781744/1‑150 (MQ=255)
                                                                          aTTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGt                                                                          <  2:1442710/150‑1 (MQ=255)
                                                                             tGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCCCGCTTATGTCAAGCGTGCGGTCCGTGGATTCAACCCGAGACCGCCTGCCACGAGTCAGAGGAAACGACGGATATGTTCAGTCgg                                                                       >  1:1398604/1‑149 (MQ=255)
                                                                             tGTCATCACAATGATGGTACCTTATTTCAGACACCATTTTTTCAACTCCGCTTTCCACGGACCGCACTCTTATGTCAAGAGGGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGt                                                                       <  2:760446/150‑1 (MQ=255)
                                                                                catcaCAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTaaa                                                                    >  2:895330/1‑150 (MQ=255)
                                                                                 atcaCAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTaaaa                                                                   <  2:227798/150‑1 (MQ=255)
                                                                                     caATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTATTATTTAAAGATTGCGGTCAGTGGCTACAACCAGAGACCGACTTAAACGAGTCAGAGGAAACGACGCAAATGTTCAGTCGTAAAatata                                                               >  2:572192/1‑149 (MQ=255)
                                                                                      aaTGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAAtatct                                                              <  2:785131/150‑1 (MQ=255)
                                                                                       atgatgGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAAtatcta                                                             >  2:130867/1‑150 (MQ=255)
                                                                                          atgGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCa                                                          <  2:403685/150‑1 (MQ=255)
                                                                                           tgGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTTTGTCAAGAGTGCGGGCCGTGGATACCACCAGAGACCGACAGACACGAGTCAGGGGAAACGACGGATAAGTTCAGTCGGAAAATATATATCaa                                                         >  2:1095798/1‑150 (MQ=255)
                                                                                                ccTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAAc                                                    <  1:1139920/150‑1 (MQ=255)
                                                                                                    cttTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGa                                                >  1:312119/1‑150 (MQ=255)
                                                                                                       tCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGCCACGAGTCAGAGGAAACGCCGGATATGTTCAATAGTAAAATATCAATCAAAAAACATGATTa                                             >  1:861899/1‑150 (MQ=255)
                                                                                                         aGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGCCCGACTGACACGAGTCAGAGGAAAAGACGGATATTTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAg                                           >  1:1703242/1‑150 (MQ=255)
                                                                                                         aGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAg                                           >  2:631443/1‑150 (MQ=255)
                                                                                                          gACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAgg                                          <  2:1098258/150‑1 (MQ=255)
                                                                                                          gACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAgg                                          >  1:1048035/1‑150 (MQ=255)
                                                                                                          gACACCATTTTTTCAACTCCGTTATCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAgg                                          <  1:1435112/150‑1 (MQ=255)
                                                                                                           acacCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGt                                         <  1:140949/150‑1 (MQ=255)
                                                                                                            cagcATTTTTTCAACTCCGTTTTCCACTGACCGCACTCTTATGTCAAGAGTGCGGTCTGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTc                                        <  1:962643/147‑1 (MQ=255)
                                                                                                            cacCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTc                                        >  2:585566/1‑150 (MQ=255)
                                                                                                             acCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGCCCGACTGCCACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCa                                       >  1:1382921/1‑150 (MQ=255)
                                                                                                              ccATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCaa                                      >  1:955957/1‑150 (MQ=255)
                                                                                                                  tttttCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAAt                                  <  1:540479/150‑1 (MQ=255)
                                                                                                                      tCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACAAGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGttt                              <  1:1140938/150‑1 (MQ=255)
                                                                                                                       cAACTCCGTTTTCCACGGACCGCACTCGTATGTCAAGAGTGCGTTCCGTGGATACAAACATAGACCGACTGACACGAGTCAGATGAAACCACGGATATGTTCAGTCGTAAAATATCTATCAAAAAAGATTATAAAGGTCAAAAATttttt                             >  2:1362643/1‑149 (MQ=255)
                                                                                                                           tCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGatat                         >  1:234992/1‑150 (MQ=255)
                                                                                                                            ccGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCTTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAAAGTTTGATAtt                        >  2:1168781/1‑150 (MQ=255)
                                                                                                                              tttttCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGAAAGACACGAGTCAGAGGAAACGACGGATATGTTCAGTATTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTa                      <  1:1124781/149‑1 (MQ=255)
                                                                                                                               ttttCCACTGACCGAACTCTTATGTCAAGAGTGCGGTACGTGGATACAACCAGAGACCTACTGACACGAGTCAGAGTAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGGCAAAAATGTTTGATATTTAc                     <  1:273664/150‑1 (MQ=255)
                                                                                                                               ttttCCACGGACCGCGCTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTAc                     >  1:898742/1‑150 (MQ=255)
                                                                                                                               ttttCCACGGACCGCACTCTTATGTCAATAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCATAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTAc                     <  2:1534298/150‑1 (MQ=255)
                                                                                                                               ttttCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTAc                     <  2:1716620/150‑1 (MQ=255)
                                                                                                                                   ccACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAAtt                 <  2:601988/150‑1 (MQ=255)
                                                                                                                                   ccACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGATTGATATTTAAAAtt                 >  2:277800/1‑150 (MQ=255)
                                                                                                                                    cACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAAttt                >  2:932839/1‑150 (MQ=255)
                                                                                                                                      cGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTAt              <  2:358104/150‑1 (MQ=255)
                                                                                                                                      cGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTAt              <  1:1095798/150‑1 (MQ=255)
                                                                                                                                       ggACCGCACTCTTGACATAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGCCACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACATTTTATg             >  2:1023580/1‑150 (MQ=39)
                                                                                                                                        gACCGCACTCTTGACATAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGa            >  2:1542993/1‑150 (MQ=255)
                                                                                                                                         aCCGCACTCTTGACATAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGaa           >  1:1468504/1‑150 (MQ=255)
                                                                                                                                         aCCGCACTCTTGACATAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGaa           >  1:1034194/1‑150 (MQ=255)
                                                                                                                                            gCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGac        >  2:488417/1‑149 (MQ=255)
                                                                                                                                            gCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGAt        <  1:1210464/150‑1 (MQ=255)
                                                                                                                                            gCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGAt        >  1:1274355/1‑150 (MQ=255)
                                                                                                                                            gCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGAt        >  2:1553235/1‑150 (MQ=255)
                                                                                                                                            gCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGAt        >  1:1030207/1‑150 (MQ=255)
                                                                                                                                            gCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGATTGATATTTACAATTTATGAAGAt        >  2:1516090/1‑150 (MQ=255)
                                                                                                                                                tctTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACa    <  1:596657/150‑1 (MQ=255)
                                                                                                                                                  ttATGTCAAGAGTGCGGTGCGTGGATACAACCAGAGACCGAATGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACaat  <  1:1643527/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GACCTGATACTGATATGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACAAT  >  NC_000913/2068259‑2068554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: