Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,068,409 G→C 9.4% pseudogene (11/159 nt) yoeH ← pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,068,4090GC 9.4% 302.6 / 21.9 117pseudogene (11/159 nt)yoeHpseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base G (61/43);  new base C (4/7);  total (67/50)
Fisher's exact test for biased strand distribution p-value = 2.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-02

Only 100 of 122 total aligned reads displayed.

CCTGATACTGATATGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACAATAATT  >  NC_000913/2068261‑2068558
                                                                                                                                                    |                                                                                                                                                     
ccTGATACTGATATGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGt                                                                                                                                                      <  1:1281/150‑1 (MQ=255)
   gatactgataTGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCaa                                                                                                                                                   >  2:1435112/1‑150 (MQ=255)
         gataTGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGTTACCTTCTATCAGACACCATTTTTTCAACTCAGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGc                                                                                                                                             >  1:97320/1‑150 (MQ=255)
         gataTGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACTGACCGCACTCTTATGTCAAGAGTGc                                                                                                                                             >  1:1723394/1‑150 (MQ=255)
          atatGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCg                                                                                                                                            >  2:327070/1‑150 (MQ=255)
           tatGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTGACATAAGAGTGCgg                                                                                                                                           <  1:802229/150‑1 (MQ=255)
           tatGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTGACATAAGAGTGCgg                                                                                                                                           <  1:633752/150‑1 (MQ=255)
           tatGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTGACATAAGAGTGCgg                                                                                                                                           <  1:561024/150‑1 (MQ=255)
            atGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTGACATAAGAGTGCGGt                                                                                                                                          <  1:1355367/150‑1 (MQ=255)
             tGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTc                                                                                                                                         <  2:1632991/150‑1 (MQ=255)
             tGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATAATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTc                                                                                                                                         >  2:703487/1‑150 (MQ=255)
              gAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCATTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTcc                                                                                                                                        >  2:1194827/1‑150 (MQ=255)
                 tCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTg                                                                                                                                     <  1:993100/150‑1 (MQ=255)
                 tCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTAGACATAAGAGTGCGGTCCGTg                                                                                                                                     <  1:1489797/150‑1 (MQ=39)
                  cTTACCGCATATACGGTCAATTTCAGCAATTAATAACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCAATTTTACAAATCCGTTTTCCACGGACCGCACTCTAATGTCAAGAGTGCGGTCCGTgg                                                                                                                                    >  1:1132158/1‑150 (MQ=255)
                     aCCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGCCCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATa                                                                                                                                 >  2:942227/1‑150 (MQ=255)
                       cGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACa                                                                                                                               <  2:561640/150‑1 (MQ=255)
                        gCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGAACGCACTCTTATGTAAAGAGTGCGGTCCGTGGATACaa                                                                                                                              >  1:981990/1‑150 (MQ=255)
                           tataCGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCa                                                                                                                           <  2:1237148/150‑1 (MQ=255)
                           tataCGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGAAACCATTTTTTCAACTCAGTTTTCCACGGACCGCAATATTATTTAAAGAGTTCCGTACGTGGAaacaacaa                                                                                                                           >  1:603022/1‑148 (MQ=255)
                            ataCGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCag                                                                                                                          >  1:227798/1‑150 (MQ=255)
                            ataCGGTCAATTTCAGCAATTAATTACATTAACCACGCCAAAGTATTTGTCATCACAATGATGTTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCag                                                                                                                          <  1:1524220/150‑1 (MQ=255)
                              aCGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTAAAGAGTGCGGTCCGTGGATACAACCagag                                                                                                                        >  2:596657/1‑150 (MQ=255)
                                ggTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGAc                                                                                                                      <  2:457514/150‑1 (MQ=255)
                                ggTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACAGCACTCTTATGTCAAGAGTCCGGTCCCTGGAAACAACCAGAAAc                                                                                                                      >  1:478125/1‑150 (MQ=255)
                                 gTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTAATCACAATGATGGTACCTTCTTTAATACACCATTTTTTAAACTAAGTATTCAACGGACAGCACTATTAAGACAAGAGTGATCTCCGTGTATACAACCagataac                                                                                                                     >  1:1216507/1‑146 (MQ=255)
                                    aaTTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTACGTGGATACAACCAGAGACCGaa                                                                                                                  >  1:1372579/1‑149 (MQ=255)
                                     aTTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACt                                                                                                                 <  2:278442/150‑1 (MQ=255)
                                      tttCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTg                                                                                                                <  1:913779/150‑1 (MQ=255)
                                      tttCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTg                                                                                                                <  2:756802/150‑1 (MQ=255)
                                          aGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACCGACCGCACTCTTATGTAAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGacac                                                                                                            >  1:367275/1‑150 (MQ=255)
                                           gCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGacacc                                                                                                           >  1:624283/1‑149 (MQ=255)
                                           gCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACg                                                                                                           >  1:55259/1‑150 (MQ=255)
                                           gCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACACTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACg                                                                                                           >  1:1002756/1‑150 (MQ=255)
                                            cAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGa                                                                                                          >  1:1441753/1‑150 (MQ=255)
                                               ttaattaCATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTc                                                                                                       <  1:1722493/150‑1 (MQ=255)
                                                  attaCATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCaga                                                                                                    <  2:1354622/150‑1 (MQ=255)
                                                  attaCATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACGCGGATGGCAAGACTGCGGTGCGTGGTTACAACCAGATACCGGCAGCCCCGAGTCaga                                                                                                    >  2:1497724/1‑150 (MQ=255)
                                                     aCATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGa                                                                                                 >  2:668017/1‑150 (MQ=255)
                                                             cACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACgg                                                                                         >  1:590045/1‑150 (MQ=255)
                                                              aCGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTAAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGa                                                                                        >  1:1113478/1‑150 (MQ=255)
                                                               cGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTACGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAAAGACGGat                                                                                       >  2:195120/1‑150 (MQ=255)
                                                                gCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGata                                                                                      >  1:1189087/1‑150 (MQ=255)
                                                                  cAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATg                                                                                    >  1:358104/1‑150 (MQ=255)
                                                                   aaaGTATTTGTCATCACAATGATGGTACCTCCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGt                                                                                   >  2:768198/1‑150 (MQ=255)
                                                                     aGTATTTGTCATCACAATGATGGTTCCTTTTTTCAGACACCATTTTTTCAACTCCGATTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTc                                                                                 <  2:1688265/150‑1 (MQ=255)
                                                                       tATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCGTATGTCAAGAGTGCGGTCCGTGGCTAAAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAg                                                                               >  1:1362067/1‑150 (MQ=255)
                                                                        aTTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGt                                                                              >  1:1392990/1‑150 (MQ=255)
                                                                           tGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCCCGCTTATGTCAAGCGTGCGGTCCGTGGATTCAACCCGAGACCGCCTGCCACGAGTCAGAGGAAACGACGGATATGTTCAGTCgg                                                                           >  1:1398604/1‑149 (MQ=255)
                                                                           tGTCATCACAATGATGGTACCTTATTTCAGACACCATTTTTTCAACTCCGCTTTCCACGGACCGCACTCTTATGTCAAGAGGGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGt                                                                           <  2:760446/150‑1 (MQ=255)
                                                                            gTCAGCACACTGATGGTACCTTCTTTCAGACACCATTTTTTCACCTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTa                                                                          <  1:116116/150‑1 (MQ=255)
                                                                              catcaCAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTaaa                                                                        >  2:895330/1‑150 (MQ=255)
                                                                              catcaCAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTaaa                                                                        >  2:615616/1‑150 (MQ=255)
                                                                                    aaTGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACTGACCGCACTCTTATGTCAAGAGTGCGGTCCGAGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAAtatct                                                                  <  1:327070/150‑1 (MQ=255)
                                                                                    aaTGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAAtatct                                                                  <  2:785131/150‑1 (MQ=255)
                                                                                     atgatgGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAAtatcta                                                                 >  2:130867/1‑150 (MQ=255)
                                                                                        atgGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCa                                                              <  2:403685/150‑1 (MQ=255)
                                                                                         tgGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTTTGTCAAGAGTGCGGGCCGTGGATACCACCAGAGACCGACAGACACGAGTCAGGGGAAACGACGGATAAGTTCAGTCGGAAAATATATATCaa                                                             >  2:1095798/1‑150 (MQ=255)
                                                                                              ccTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAAc                                                        <  1:1139920/150‑1 (MQ=255)
                                                                                                  cttTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGa                                                    >  1:312119/1‑150 (MQ=255)
                                                                                                     tCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGCCACGAGTCAGAGGAAACGCCGGATATGTTCAATAGTAAAATATCAATCAAAAAACATGATTa                                                 >  1:861899/1‑150 (MQ=255)
                                                                                                        gACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAgg                                              >  1:1048035/1‑150 (MQ=255)
                                                                                                        gACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAgg                                              <  2:1098258/150‑1 (MQ=255)
                                                                                                        gACACCATTTTTTCAACTCCGTTATCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAgg                                              <  1:1435112/150‑1 (MQ=255)
                                                                                                         acacCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGt                                             <  2:546053/150‑1 (MQ=255)
                                                                                                          cagcATTTTTTCAACTCCGTTTTCCACTGACCGCACTCTTATGTCAAGAGTGCGGTCTGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTc                                            <  1:962643/147‑1 (MQ=255)
                                                                                                          cacCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTc                                            >  2:585566/1‑150 (MQ=255)
                                                                                                          cacCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTAAGTCGTAAAATATCTATCAAAAAACATGATTAATGTc                                            >  2:325425/1‑150 (MQ=255)
                                                                                                           acCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGCCCGACTGCCACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCa                                           >  1:1382921/1‑150 (MQ=255)
                                                                                                            ccATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCaa                                          >  1:955957/1‑150 (MQ=255)
                                                                                                                tttttCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAAt                                      <  1:540479/150‑1 (MQ=255)
                                                                                                                     cAACTCCGTTTTCCACGGACCGCACTCGTATGTCAAGAGTGCGTTCCGTGGATACAAACATAGACCGACTGACACGAGTCAGATGAAACCACGGATATGTTCAGTCGTAAAATATCTATCAAAAAAGATTATAAAGGTCAAAAATttttt                                 >  2:1362643/1‑149 (MQ=255)
                                                                                                                         tCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGatat                             >  1:234992/1‑150 (MQ=255)
                                                                                                                          ccGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCTTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAAAGTTTGATAtt                            >  2:1168781/1‑150 (MQ=255)
                                                                                                                            tttttCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGAAAGACACGAGTCAGAGGAAACGACGGATATGTTCAGTATTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTa                          <  1:1124781/149‑1 (MQ=255)
                                                                                                                            gTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTa                          >  1:917673/1‑150 (MQ=255)
                                                                                                                             ttttCCACTGACCGAACTCTTATGTCAAGAGTGCGGTACGTGGATACAACCAGAGACCTACTGACACGAGTCAGAGTAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGGCAAAAATGTTTGATATTTAc                         <  1:273664/150‑1 (MQ=255)
                                                                                                                             ttttCCACGGACCGCGCTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTAc                         >  1:898742/1‑150 (MQ=255)
                                                                                                                             ttttCCACGGACCGCACTCTTATGTCAATAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCATAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTAc                         <  2:1534298/150‑1 (MQ=255)
                                                                                                                             ttttCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTAc                         <  2:1716620/150‑1 (MQ=255)
                                                                                                                                 ccACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAAtt                     <  2:601988/150‑1 (MQ=255)
                                                                                                                                 ccACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGATTGATATTTAAAAtt                     >  2:277800/1‑150 (MQ=255)
                                                                                                                                  cACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAAttt                    >  2:932839/1‑150 (MQ=255)
                                                                                                                                    cGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTAt                  <  1:1095798/150‑1 (MQ=255)
                                                                                                                                       aCCGCACTCTTGACATAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGaa               >  1:1468504/1‑150 (MQ=255)
                                                                                                                                       aCCGCACTCTTGACATAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGaa               >  1:1034194/1‑150 (MQ=255)
                                                                                                                                        ccGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAg              <  1:1104120/150‑1 (MQ=255)
                                                                                                                                          gCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGac            >  2:488417/1‑149 (MQ=255)
                                                                                                                                          gCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGAt            >  2:1553235/1‑150 (MQ=255)
                                                                                                                                          gCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGAt            >  1:1030207/1‑150 (MQ=255)
                                                                                                                                          gCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGAt            >  1:1274355/1‑150 (MQ=255)
                                                                                                                                          gCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGAt            <  1:1210464/150‑1 (MQ=255)
                                                                                                                                          gCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGATTGATATTTACAATTTATGAAGAt            >  2:1516090/1‑150 (MQ=255)
                                                                                                                                          gCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGACAAAAATGTTTGATATTTAAAATTTATGAAGAt            >  1:1274364/1‑150 (MQ=255)
                                                                                                                                              tctTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACa        <  1:596657/150‑1 (MQ=255)
                                                                                                                                                ttATGTCAAGAGTGCGGTGCGTGGATACAACCAGAGACCGAATGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACaat      <  1:1643527/150‑1 (MQ=255)
                                                                                                                                                    gTCAAGAGTGCGGTCCGTGTATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACAATAAtt  <  1:1623082/150‑1 (MQ=255)
                                                                                                                                                    gTCAAGAGTGCGGTCCGTGGATACGACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAATACATGATTAAGGTCAAAATTGTTTGATATTTACAATTTTTGAATATGACAattatt  >  1:1603342/1‑150 (MQ=255)
                                                                                                                                                    gTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGATACGACGGATATGTTCAGTCGTAAACTATCTATCACAAACCAGGAGTAAGGGCAAAAATGTTTGAGATTTACAATTTATGAAGATGACAATAAtt  >  1:1596910/1‑150 (MQ=255)
                                                                                                                                                    gTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATACCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACAATAAtt  >  2:1463051/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CCTGATACTGATATGAGTCTTACCGCATATACGGTCAATTTCAGCAATTAATTACATTATCCACGCCAAAGTATTTGTCATCACAATGATGGTACCTTCTTTCAGACACCATTTTTTCAACTCCGTTTTCCACGGACCGCACTCTTATGTCAAGAGTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACAATAATT  >  NC_000913/2068261‑2068558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: