Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,074,691 C→G 7.8% intergenic (+33/‑88) flu → / → yeeR CP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter/CP4‑44 prophage; putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,074,6910CG 7.8% 267.5 / 12.9 102intergenic (+33/‑88)flu/yeeRCP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter/CP4‑44 prophage; putative membrane protein
Reads supporting (aligned to +/- strand):  ref base C (48/44);  new base G (3/5);  total (53/49)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.56e-01

Only 100 of 107 total aligned reads displayed.

GAAGCCCGTGTCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCATTCTTCGCCTTT  >  NC_000913/2074542‑2074839
                                                                                                                                                     |                                                                                                                                                    
gAAGCCCGTGTCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGtcatc                                                                                                                                                      >  2:238648/1‑150 (MQ=255)
    cccGTGTCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGa                                                                                                                                                  >  1:829369/1‑150 (MQ=255)
        tgtCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCgg                                                                                                                                              <  2:156042/150‑1 (MQ=255)
         gtCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCggg                                                                                                                                             >  2:414005/1‑150 (MQ=255)
           ccGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATGATGACCGGGAc                                                                                                                                           <  1:400646/150‑1 (MQ=255)
           ccGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATGATGACCGGGAc                                                                                                                                           <  1:415840/150‑1 (MQ=255)
           ccGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATGATGACCGGGAc                                                                                                                                           <  1:1288777/150‑1 (MQ=255)
           ccGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGAATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATGATGACCGGGAc                                                                                                                                           <  1:1295592/150‑1 (MQ=255)
            cGGGAAGATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCCGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGCCAGAACCATCGCCTCTCTGTGGTCCCGGGCATGAAGGCCGGGccc                                                                                                                                          >  1:1440356/1‑150 (MQ=255)
            cGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGAcc                                                                                                                                          >  1:313463/1‑150 (MQ=255)
            cGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATAACCGGGAcc                                                                                                                                          >  2:1060813/1‑150 (MQ=255)
              ggAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGGCCACCTGACCGGGGCCCg                                                                                                                                        >  1:425622/1‑150 (MQ=255)
                  aaTATCACCCGGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGACCCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGAcc                                                                                                                                    <  1:1176808/150‑1 (MQ=255)
                     atCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCCCACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGgc                                                                                                                                 >  2:121141/1‑150 (MQ=255)
                     atCACACTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGgc                                                                                                                                 >  1:1031119/1‑150 (MQ=255)
                      tCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGCACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGgcg                                                                                                                                >  1:1114907/1‑150 (MQ=255)
                         cccTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCaa                                                                                                                             >  2:643139/1‑150 (MQ=255)
                           cTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGATCATCATGACCGGGACCCGGACCGGCGCAACg                                                                                                                           <  2:1613106/150‑1 (MQ=255)
                               gCGTTCAGGCCGTTTATGCCCACTGCGTCAGCTGCAGCTGCGCTGATGGTTATAAGGGTCTGGCAACACTTAATGTGACCTTCTCACAGAACCATCGCCTCTCTGTGGGCCCGGTCATCATCACGGGGACCCGGACCGGCGCAACGGATc                                                                                                                       <  2:1474957/150‑1 (MQ=255)
                                gtttatggCCGTTTATGCCACGCGCGCCTGGGGCTGCAGCGGTTATGGTTAAAATGGTCTGCCCACATTTAATGTGACCTTGTTCCACAACCAGCGCCTATCTGGGGGCCCGTGAAACATGACGGGGACCCGGACCGGCGCAACGGATCt                                                                                                                      <  2:1281478/144‑1 (MQ=255)
                                   tCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCa                                                                                                                   <  2:1200830/150‑1 (MQ=255)
                                    cAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCaa                                                                                                                  >  1:897799/1‑150 (MQ=255)
                                      ggcggtttATGCCCACAGCGTCAGCGGCTGCAGCGCGGAAGGTTTTACGGGTCTGGCCACACGTTTTGTGACGTTCTGACTGAACCTTCGCCACTCTGTGGTCCGGGTCATCATGACCGGTACCCGGACCGGCGCAACGGATCTTCAACg                                                                                                                <  2:829369/144‑1 (MQ=255)
                                       gCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGCCCCGGACCGGCGCAACGGATCTTCAACGc                                                                                                               >  2:618960/1‑150 (MQ=255)
                                          ggTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCcag                                                                                                            <  1:887913/150‑2 (MQ=255)
                                             tATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACAtt                                                                                                         >  2:811066/1‑150 (MQ=255)
                                                 cccACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATAATGAACGGGACCCGGCCCGGCGCAACCGCTCTTAAAACCCACATTCGcg                                                                                                     >  1:1714874/1‑149 (MQ=255)
                                                   cacaGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCtt                                                                                                                     <  1:494015/132‑1 (MQ=255)
                                                   cacaGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCtt                                                                                                                     >  2:494015/1‑132 (MQ=255)
                                                    ccaGCGTCAGCGGCAGCAGCGCTGAAGTGAATAACGGTCAGGCCACACTGAATGTGACCTTCTCCCAGAACCAGCGCCTCTGTGTGGGCCCGGTCATCATGCCCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGc                                                                                                  <  1:1397537/149‑1 (MQ=255)
                                                    acaGCGTCCGGGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTCAATGTCACCTTCTGACACAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACGGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGc                                                                                                  <  1:1399321/150‑1 (MQ=255)
                                                      aGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACCTTCGCGGGCAt                                                                                                >  1:1605806/1‑150 (MQ=255)
                                                         gTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTaa                                                                                             >  1:987454/1‑150 (MQ=255)
                                                           ttggggCTGCAGGGATGAAGGGTATAACGGTCAGGACAAACTGATTGTGACCTTATGACAGTACCATGGCCTCTCTGTGGTCCGGGTCATCATGAGCGGGACGCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACa                                                                                           <  2:1697171/146‑1 (MQ=255)
                                                           cAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACa                                                                                           <  1:26312/150‑1 (MQ=255)
                                                           cAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACa                                                                                           <  2:895407/150‑1 (MQ=255)
                                                            aGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCCTCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATGAACaa                                                                                          >  1:276282/1‑150 (MQ=255)
                                                              cGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATa                                                                                        >  1:954823/1‑150 (MQ=255)
                                                               ggCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGACCCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCCCATTCGCTGGCATTAACAATaa                                                                                       >  1:1028062/1‑150 (MQ=255)
                                                               ggCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCGCCGGCTCTTAAACGCCACATTCGCGGGCATTATCAATaa                                                                                       >  1:1579221/1‑150 (MQ=255)
                                                               ggCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCACCGGATCTTCAACGCCACATTCGCTGGCATTAACAATaa                                                                                       >  1:44703/1‑150 (MQ=255)
                                                                 cagcagCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGCCCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACa                                                                                     >  2:60538/1‑150 (MQ=255)
                                                                 cagcagCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTAATCAAGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCCCATTCGCTGGCATTAACAATAACa                                                                                     >  2:671228/1‑150 (MQ=255)
                                                                  agcagcGCTGATGGTTATAACGGTCAGGCCACAGTGATTGTGACCTTCATACAGAACCATCGCCTCTGTGTGGTCCCGTGCATCATGACCGGTACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACAt                                                                                    <  1:1640506/150‑1 (MQ=255)
                                                                  agcagcGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCGACATTCGCTGGCATTAACAATAACAt                                                                                    >  2:1232810/1‑150 (MQ=255)
                                                                   gccgggcTTAAGGGTATAAGGGTCAGGCCACAGTTACTGTTACCTGCGGACCGAACCATCGCCTCTCTGGGGTCCGGGGCTTCTTGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATg                                                                                   <  2:1569990/145‑1 (MQ=255)
                                                                    cagcGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGACCCATCGCCTCTCTGTGGTCCCGGTGATCATGACCGGGCCCCGGGCCGGCGGAACGGGTCTTCAACGCCACCTTCGCTGGCATTTACAATAACATGa                                                                                  >  2:1527934/1‑150 (MQ=255)
                                                                    cagcGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGa                                                                                  <  2:931825/150‑1 (MQ=255)
                                                                     agcGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCGTCAACGCCACCTTCGCGGGCATTTACAATAACATGat                                                                                 >  1:681947/1‑150 (MQ=255)
                                                                     agcGCTGAAGGGTAAAACGGTCAGGCCACACTGAATTTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGat                                                                                 <  2:934152/150‑1 (MQ=255)
                                                                          tgatgtttataAGGGTCAGGCAACAAGGAATGTCAGATTCTGACAGAACCATCGCATGTATGGGGTCCTGGTAATCATGACGGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATtca                                                                            <  2:1490801/143‑1 (MQ=255)
                                                                                gTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTATCAATAACATGATATTCATCACgg                                                                      <  2:1139454/150‑1 (MQ=255)
                                                                                gTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACgg                                                                      <  2:416331/150‑1 (MQ=255)
                                                                                gTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTAATAATGACCGGGACCCGGCCCGGCGCAACGGGTCTTCAACGCCACATTCGCGGGCATTTACAATAACATGATTTTGATAACgg                                                                      >  1:1567294/1‑150 (MQ=255)
                                                                                   taACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGt                                                                   >  1:660532/1‑150 (MQ=255)
                                                                                    tgtcagaaGGTCACATTCAGTGTGGCCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACAGGATATTCATCACGGAGTg                                                                  >  1:1049531/8‑150 (MQ=255)
                                                                                      cGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCACCGCCACCTTCGCTGGCATTAACAATAACAAGATATTCATCACGGAGTGAc                                                                >  2:239639/1‑150 (MQ=255)
                                                                                      cGGTCAGGCCACACTCAATGTCACCTTCTGACCGAACCACCGCCTCTCTGGGGCCCCGGCCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGAc                                                                <  2:409529/150‑1 (MQ=255)
                                                                                        gTCTGGCCACACTGAATGTTACCTTCTGACACAACCATCGCCTCTCTGTGGTCCCGTGCCTCATGCCCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATAATCATCACGGAGTGACTa                                                              <  1:1620476/150‑1 (MQ=255)
                                                                                        gTCAGGCCACACTTAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTa                                                              <  2:1192388/150‑1 (MQ=255)
                                                                                            ggCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTTATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGtt                                                          >  1:18979/1‑150 (MQ=255)
                                                                                             gCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTTGCATTAACAATAACATGATATTCATCCCGGAGTGACTATGTTa                                                         >  2:1086066/1‑150 (MQ=255)
                                                                                             gCCACACTGAAGGTGACCTTCTGACACAACCATCCCCTCTCTGTGGCCCCGTTAATCATGCCCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTa                                                         <  1:555568/150‑1 (MQ=255)
                                                                                              ccACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATATTAc                                                        <  2:308181/150‑1 (MQ=255)
                                                                                               cacaCTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGCCCCGGACCGGCGGAGGGGTTCTTAAACGCCACAGTCGCTGGCATGTACAAGGACATGATATTCATCACGGAGTGCATATGTTaga                                                       >  2:252667/1‑148 (MQ=255)
                                                                                               cacaCTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGCCCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACa                                                       >  1:1011880/1‑150 (MQ=255)
                                                                                               cacaCTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACAGGGACCCGGCCCGGCGCAACGGATCTTCAACGACGCATTGGCTGGCATTTACAATATCAGGAGATTCTTCAGGGAGTGACTATGTTACa                                                       >  2:1113580/1‑150 (MQ=255)
                                                                                                acacTGAATGTGAACTACTGACAGAACCATCGCCTCTCTGTGATCCCGGACATCAAGACCGGGACCCGGACCGGCGCAACCGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAg                                                      >  1:1122952/1‑150 (MQ=255)
                                                                                                  acTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACAGTCGCTGGCATTAACAATAACAAGATATTAATCACCGCGTGACGATTTTACAGAt                                                    >  2:871948/1‑150 (MQ=255)
                                                                                                       aTGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCg                                               >  2:1018686/1‑150 (MQ=255)
                                                                                                         gtgACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGgc                                             <  1:1703064/150‑1 (MQ=255)
                                                                                                           gACCTTCTGACAGAACCATCGCCTCTCTGTGGGCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGgcgc                                           <  1:229452/150‑1 (MQ=255)
                                                                                                            aCCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGgcgcg                                          <  1:640384/150‑1 (MQ=255)
                                                                                                              cTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAAGGGATCTGCAACGCCACATGCGCTGGCAGTAACAATATCATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCt                                        <  1:268168/150‑1 (MQ=255)
                                                                                                                 cTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACAATGTTACAGATAGTCGGCGCGCTGAt                                     >  2:900878/1‑150 (MQ=255)
                                                                                                                  tGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCGACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATc                                    >  1:1173759/1‑150 (MQ=255)
                                                                                                                   gACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATcc                                   <  2:57260/150‑1 (MQ=255)
                                                                                                                     cAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCAGTAACAATAACATGATATTCATCACGGAGTGACTTTGTTACAGATAGTCGGCGCGCTGATCctg                                 >  2:76706/1‑150 (MQ=255)
                                                                                                                     cAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCCGACCGGCGCAACGGATATTCAACCCCACATTCGCTGGCATTAACAATAACATGATATTAATCACGGAGTGACTATGTTACAGATATTCGGCGCGCTGATCctg                                 >  1:377757/1‑150 (MQ=255)
                                                                                                                     cAGAACCATCGCCTCTCTGTGGTCCCCGTCATCATTACCGGGACCCGGAACGGCGCAACGGATCTTCAACGCCACCTTAGCTTGCATTTACCATAAAATGATATTAATCACGGAGTGACTTTGGTACAGATAGTAGGCGCGCTTTTCctg                                 >  2:871313/1‑150 (MQ=255)
                                                                                                                     cAGAACCATCGCCTCTCTGTGGTCCCCGTCATCATGACCGGGACCCGGCCCGGCGCAACGGATCTTCAACGCCCCATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTTCAGATTGTCGGCGCGCTGATCctg                                 >  2:578962/1‑150 (MQ=255)
                                                                                                                        aaCCATCGCCTCTCTGTGGTCCCGGTCATCATGCCCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCctgctg                              <  1:414005/150‑1 (MQ=255)
                                                                                                                         aCCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGa                             >  1:103314/1‑150 (MQ=255)
                                                                                                                           cATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATc                           <  1:1064608/150‑1 (MQ=255)
                                                                                                                               gCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAg                       <  1:1720161/150‑1 (MQ=255)
                                                                                                                               gCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAg                       <  1:121141/150‑1 (MQ=255)
                                                                                                                                 ctctctGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGa                     >  2:226337/1‑150 (MQ=255)
                                                                                                                                     ctGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACAAGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGAACCTGCTGATCGCAGGATTTg                 <  2:853536/150‑1 (MQ=255)
                                                                                                                                     ctGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAAAAACATGATATTCATCACGGAGTGACTATGTTAAAGATAGTCGGCGCGCTGATCCGGCTGATCGCAGGAtttt                 >  1:1371043/1‑149 (MQ=255)
                                                                                                                                       gtgGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGcc               <  2:1031119/150‑1 (MQ=255)
                                                                                                                                       gtgGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGACGGCGCGCTGATCCTGCTGATCGCAGGATTTGcc               <  2:500980/150‑1 (MQ=255)
                                                                                                                                       cgggTCCCGGTCATGATGACCGGGCCCCGGACCGGCGCAACGGATCTTCAACGCCCCATTCGCGGGCATTAACAATAACATGATATTGATCAGGGAGTGCCTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGcc               >  1:1730257/3‑150 (MQ=255)
                                                                                                                                          gTCCCGGTCATGATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCAtt            >  2:1682823/1‑150 (MQ=255)
                                                                                                                                          gTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCAtt            >  2:840221/1‑150 (MQ=255)
                                                                                                                                               ggTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCATTCTTCg       <  2:103314/150‑1 (MQ=255)
                                                                                                                                                gTCATCATGCCCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCATTCTTCGc      <  2:1058546/150‑1 (MQ=255)
                                                                                                                                                 tcatcaTGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCg                          >  1:1221567/1‑129 (MQ=255)
                                                                                                                                                 tcatcaTGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCg                          <  2:1221567/129‑1 (MQ=255)
                                                                                                                                                  catcatGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCATTCTTCGCCt    <  2:1398303/150‑1 (MQ=255)
                                                                                                                                                    tcatGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCATTCTTCGCCttt  >  2:173012/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GAAGCCCGTGTCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCATTCTTCGCCTTT  >  NC_000913/2074542‑2074839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: