Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,078,301 A→T 6.0% intergenic (+194/+248) yeeW → / ← yoeD CP4‑44 prophage; uncharacterized protein;Phage or Prophage Related/pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,078,3010AT 6.0% 340.2 / 7.2 113intergenic (+194/+248)yeeW/yoeDCP4‑44 prophage; uncharacterized protein;Phage or Prophage Related/pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base A (59/47);  new base T (5/2);  total (64/49)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 115 total aligned reads displayed.

GGTATAGTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGCATTGACCTGAC  >  NC_000913/2078153‑2078450
                                                                                                                                                    |                                                                                                                                                      
ggTATAGTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGaa                                                                                                                                                       <  2:326254/150‑1 (MQ=255)
  tataGTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTAACCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATa                                                                                                                                                     >  1:1146739/1‑150 (MQ=255)
    aaGTGCCATAAACCACAGAAACAGCACTGTTGTCCTGTATAAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCTCCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACa                                                                                                                                                   <  1:216343/149‑1 (MQ=255)
     aGTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAg                                                                                                                                                 <  1:1076567/150‑1 (MQ=255)
      gTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGc                                                                                                                                                <  1:1136134/150‑1 (MQ=255)
      gTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACACTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAga                                                                                                                                                >  1:1337097/1‑149 (MQ=255)
          cATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGCATCTCCCTCAACAGGGTTCATTTCTCCTGACGCAAGCCTGAATACAGCTGGc                                                                                                                                            >  2:13716/1‑150 (MQ=255)
          cATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGc                                                                                                                                            >  1:734231/1‑150 (MQ=255)
             tAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCTGTATTCAGGCTGGCGtc                                                                                                                                         <  1:1266704/150‑2 (MQ=255)
               aCCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCTGTATTCAGGCTGGCGtcag                                                                                                                                       <  2:905093/150‑4 (MQ=255)
                ccACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTc                                                                                                                                      <  2:781057/150‑1 (MQ=255)
                 cacaGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCa                                                                                                                                     >  1:362488/1‑150 (MQ=255)
                    aGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCAttt                                                                                                                                  <  2:1226918/150‑1 (MQ=255)
                    aGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTATGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCAttt                                                                                                                                  >  1:336197/1‑150 (MQ=255)
                     gAAACAGCACTGTTGTCCTTTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTta                                                                                                                                 <  1:1445201/150‑1 (MQ=255)
                         cAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATaaa                                                                                                                             >  1:1656120/1‑150 (MQ=255)
                          aGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAAc                                                                                                                            <  1:469386/150‑1 (MQ=255)
                           gCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACa                                                                                                                           <  2:70501/150‑1 (MQ=255)
                                   gtCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGAGTTTTCATTTATAAACAGAAAGGaa                                                                                                                   >  1:862702/1‑150 (MQ=255)
                                     ccTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGACAGGaaaa                                                                                                                 >  2:1262727/1‑150 (MQ=255)
                                     ccTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGaaaa                                                                                                                 >  2:616847/1‑150 (MQ=255)
                                     ccTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTACATTTATAAACAGAAAGGaaaa                                                                                                                 >  1:564602/1‑150 (MQ=255)
                                      cTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAAc                                                                                                                <  1:1726376/150‑1 (MQ=255)
                                      cTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAAc                                                                                                                >  1:1732662/1‑150 (MQ=255)
                                       tGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAAcc                                                                                                               >  2:74113/1‑150 (MQ=255)
                                            tAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGataa                                                                                                          >  1:290348/1‑150 (MQ=255)
                                            tAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAAGCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGataa                                                                                                          <  2:48224/150‑1 (MQ=255)
                                             aaGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGataat                                                                                                         >  1:659799/1‑150 (MQ=255)
                                              aGCAGGCAATAGAATATGTCCTTACCCTGCGTTATCTGCAGCTTATGAACAAGAACAGTTGAATCTCAATCATCAGGCTTCATTTCTCGTGAGGCCAGCGTGATTACAGCTGGCGTTTTCATTTATAAACAGAAAGGATAACCGataata                                                                                                        <  2:1669373/150‑1 (MQ=255)
                                              aGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGataata                                                                                                        <  1:901563/150‑1 (MQ=255)
                                               gCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGAtaataa                                                                                                       <  2:1018346/150‑1 (MQ=255)
                                                 aGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATaatg                                                                                                     >  1:629579/1‑150 (MQ=255)
                                                  ggCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATaatgg                                                                                                    >  1:800651/1‑150 (MQ=255)
                                                  ggCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATaatgg                                                                                                    <  2:414409/150‑1 (MQ=255)
                                                       gagaAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATAGAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATaatggaatgg                                                                                               >  2:432652/1‑150 (MQ=255)
                                                        agaagaTGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATaatggattggc                                                                                              >  1:315665/1‑149 (MQ=255)
                                                           agaTGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGAGAATCTCACTCAACAGGCATCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGc                                                                                           <  1:59506/150‑1 (MQ=255)
                                                            gaTGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGcc                                                                                          <  1:500379/150‑1 (MQ=255)
                                                            gaTGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCAGAATACAGCAGGCGTTTTCATTTATAAACAGAAAGGAAAACCGAAAATAATGGAATGGACGcc                                                                                          >  2:1632666/1‑150 (MQ=255)
                                                               gTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGCATCTAAATCAACAGGCTTCTTTTCTCCTGACGCCGGCCTGATGACCGCTGGCGTTTTGTGGTATAAACTGAAAGGAAAACCGATAATAATGGAATGGACGcccct                                                                                       >  1:251228/1‑149 (MQ=255)
                                                                  cTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGAcc                                                                                    >  1:663467/1‑150 (MQ=255)
                                                                    tACCCTGCGCCATCTGCAGCTTCTGCCCCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCAt                                                                                  >  1:105655/1‑150 (MQ=255)
                                                                    tACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCAt                                                                                  >  1:101159/1‑150 (MQ=255)
                                                                       ccTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGaa                                                                               >  2:913448/1‑150 (MQ=255)
                                                                        cTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAg                                                                              <  1:1300649/150‑1 (MQ=255)
                                                                             ccATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACg                                                                         >  2:515029/1‑150 (MQ=255)
                                                                              cATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAACACCGAGAATAATGGAATGGACGCCCCGCCCATGAAGCAACgg                                                                        >  1:714090/1‑150 (MQ=255)
                                                                               aTCTTCAGATTCTGCACCAGAACCGTTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCATCCTGAATACAGCTGGCTTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATTGACGCCCCGACCATGAAGCAACGGt                                                                       <  1:13716/150‑1 (MQ=255)
                                                                               aTCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGt                                                                       >  2:560764/1‑150 (MQ=255)
                                                                                 cTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAg                                                                     >  1:485331/1‑150 (MQ=255)
                                                                                  tGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAgg                                                                    >  2:687601/1‑150 (MQ=255)
                                                                                  tGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAgg                                                                    >  1:1534147/1‑150 (MQ=255)
                                                                                        ttCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTa                                                              <  1:449074/150‑1 (MQ=255)
                                                                                           tGCACAAGAAACGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGAGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGTAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTg                                                           <  1:844996/150‑1 (MQ=255)
                                                                                              aCCAGAACCGTTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATa                                                        <  2:1442364/150‑1 (MQ=255)
                                                                                                cAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGAaaaa                                                      >  2:792293/1‑150 (MQ=255)
                                                                                                 aGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAAc                                                     >  1:1579793/1‑150 (MQ=255)
                                                                                                  gAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAAcc                                                    <  1:1075415/150‑1 (MQ=255)
                                                                                                  gAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAAcc                                                    <  2:659799/150‑1 (MQ=255)
                                                                                                    aCCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCACGACCATGAAGCAACGGTAGGATATTACTGATAAACCtt                                                  >  1:540755/1‑150 (MQ=255)
                                                                                                     ccGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTc                                                 >  1:1627242/1‑150 (MQ=255)
                                                                                                     ccGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTc                                                 >  1:1587654/1‑150 (MQ=255)
                                                                                                     ccGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTATTCATTTATAAACAGAAAGGAAAACCGATAAAAATGGAATGGACGCCCCGACCAAGAAGCAACGGTAGGATATTACTGATAAACCTTc                                                 >  1:698572/1‑150 (MQ=255)
                                                                                                      cGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAATGGTAGGATATTACTGATAAACCTTcc                                                >  2:1454666/1‑150 (MQ=255)
                                                                                                      cGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTcc                                                <  1:337031/150‑1 (MQ=255)
                                                                                                       ggTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCa                                               <  1:1281657/150‑1 (MQ=255)
                                                                                                        gTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGTTAGGATATTACTGATAAACCTTCCAg                                              <  1:74113/150‑1 (MQ=255)
                                                                                                        gTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTATCATTTATAAACAGAAAGGAAAACGGATAATAATGGAATGGACGCACCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAg                                              <  2:270155/150‑1 (MQ=255)
                                                                                                             tctcACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAg                                         <  1:1731580/150‑1 (MQ=255)
                                                                                                             tctcACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAg                                         <  2:1337097/150‑1 (MQ=255)
                                                                                                              atcactcaACATGCTTCATTTCTCCTGACGCCTGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAgg                                        <  1:1294142/149‑1 (MQ=255)
                                                                                                                cactcaACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCAGGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTg                                      >  2:653074/1‑150 (MQ=255)
                                                                                                                  ctcaACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCg                                    <  1:560764/150‑1 (MQ=255)
                                                                                                                       cAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATg                               >  2:1082616/1‑150 (MQ=255)
                                                                                                                        aGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATgg                              <  1:170705/150‑1 (MQ=255)
                                                                                                                          gCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTTCGTTATGGcc                            >  2:443747/1‑150 (MQ=255)
                                                                                                                           cTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCg                           >  2:759198/1‑150 (MQ=255)
                                                                                                                            ttCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGt                          >  1:1606419/1‑150 (MQ=255)
                                                                                                                             tCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGCCGCCCCGCCCATGAAGCAACGGTAGGTTATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGtt                         >  2:1143814/1‑150 (MQ=255)
                                                                                                                               aTTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGtttt                       <  2:800651/150‑1 (MQ=255)
                                                                                                                                  tctcCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCTTGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAAc                    <  2:1695335/150‑1 (MQ=255)
                                                                                                                                  tctcCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAAc                    >  1:470491/1‑150 (MQ=255)
                                                                                                                                  aatgaaaaCGCCAGCTGTATTCAGGCTGGCGTTTTCATTTATAAACAGAAAGGAAACCCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACATTCCAGTGGAGGTGCGTTATGGCCGTTTTAAc                    >  1:117715/8‑150 (MQ=255)
                                                                                                                                  aatgaaaaCGCCAGCTGTATTCAGGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAAc                    >  1:1592232/8‑150 (MQ=255)
                                                                                                                                   ctcCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCAAGTGGAGGTGCGTTATGGCCGTTTTAACg                   >  2:132786/1‑150 (MQ=255)
                                                                                                                                   ctcCTGACGCCACCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACg                   <  2:1124966/150‑1 (MQ=255)
                                                                                                                                     ccTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGAt                 <  2:422596/150‑1 (MQ=255)
                                                                                                                                     ccTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCAGGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGAt                 >  1:811990/1‑150 (MQ=255)
                                                                                                                                       tGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTa               <  1:569892/150‑1 (MQ=255)
                                                                                                                                       tGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGAAGCCCCGACCATGAAGAAACGGTAGGATAATACTGATTAACATTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTa               >  2:1145460/1‑150 (MQ=255)
                                                                                                                                       aaaCGCCAGCTGTATTCAGGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTa               >  1:589516/3‑150 (MQ=255)
                                                                                                                                        gACGCCAGCCTGAATAAAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAg              <  1:432652/150‑1 (MQ=255)
                                                                                                                                          cGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGCa            >  2:331550/1‑150 (MQ=255)
                                                                                                                                          cGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGCa            <  2:101159/150‑1 (MQ=255)
                                                                                                                                          cGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGCa            <  2:105655/150‑1 (MQ=255)
                                                                                                                                           gCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGCCCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGCAt           >  2:784976/1‑150 (MQ=255)
                                                                                                                                                ccTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCATCGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGCATTGAcc      <  1:913448/150‑1 (MQ=255)
                                                                                                                                                  tGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGCATTGACCTg    >  1:542600/1‑150 (MQ=255)
                                                                                                                                                  tGAATAAAGCTTGCGTTTTCATTTATAAACAGAAAGTAAAAAATATAATAATGGAATGGACGCCCCGACCATGAATGAACGGTAGGATATAACTGATAAACCTTCCAGTGGAGGTGAGTTATGGCCGTTTTAACGATTAGCATTGACCTg    <  1:1252731/150‑1 (MQ=255)
                                                                                                                                                    aaTACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGCATTGACCTGAc  <  1:1032656/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
GGTATAGTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTACTGATAAACCTTCCAGTGGAGGTGCGTTATGGCCGTTTTAACGATTAGCATTGACCTGAC  >  NC_000913/2078153‑2078450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: