Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,081,688 A→T 14.4% I287N (ATT→AAT)  dacD ← D‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6b

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,081,6880AT14.4% 194.5 / 21.6 83I287N (ATT→AAT) dacDD‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6b
Reads supporting (aligned to +/- strand):  ref base A (28/43);  new base T (4/8);  total (32/51)
Fisher's exact test for biased strand distribution p-value = 7.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

CTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACC  >  NC_000913/2081539‑2081836
                                                                                                                                                     |                                                                                                                                                    
cTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCaaaa                                                                                                                                                      >  2:667685/1‑150 (MQ=255)
 tttACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTACGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAAt                                                                                                                                                     >  1:1221329/1‑150 (MQ=255)
  ttACCATCTAGGGTATATTTGTCTTTGATATGTGGAATTTCGGCTTTAGGTAGCTCCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACGAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAAtt                                                                                                                                                    <  1:207639/150‑1 (MQ=255)
      cATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCa                                                                                                                                                <  2:985756/150‑1 (MQ=255)
          aaGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACCgt                                                                                                                                            <  1:1298202/150‑1 (MQ=255)
           actgTATATTAGGCTTTGATAAGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGGTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACTGTGCAAATTTTGCACCgtg                                                                                                                                           <  1:186124/147‑2 (MQ=255)
           aGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACCgtg                                                                                                                                           <  1:1681200/150‑2 (MQ=255)
           aGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCgta                                                                                                                                           <  1:745627/150‑1 (MQ=255)
            gggTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGAACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTGCGTTCCGACCTTTTTCCCTCGGTGCAAATTTTGCACCgtgg                                                                                                                                          <  2:1651790/150‑3 (MQ=255)
            gggTAAATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACCgtgg                                                                                                                                          <  1:994646/150‑3 (MQ=255)
              gTATATTTGGCTTTGAAATGTGGAATTTCGGCTTTCGGAAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACCgtggga                                                                                                                                        <  1:974739/150‑5 (MQ=255)
                  atTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCTACGGTGCAAATTTTGCACCGTGGGaaaaa                                                                                                                                    <  1:166686/150‑3 (MQ=255)
                  atTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGt                                                                                                                                    >  1:1533750/1‑150 (MQ=255)
                    ttGGCTTTGATATGTTGAATTTCGGCTTTCGGTAGCACCATCGAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGttt                                                                                                                                  <  1:1217787/150‑1 (MQ=255)
                    ttGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACCGTGGGaaaaagg                                                                                                                                  <  2:1495037/150‑5 (MQ=255)
                      gtcTTAGATATGTGGAATTTCGGCTTTCGGAAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCACACGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTttg                                                                                                                                <  2:210393/148‑1 (MQ=255)
                      ggCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCTCGGTGCAAAATTTGCACCGTAGTAAAGTTttg                                                                                                                                <  1:1533060/150‑1 (MQ=255)
                       gCTTTGATATGTGGAATTTCGGCTTTCGTTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTttgt                                                                                                                               <  1:1273307/150‑1 (MQ=255)
                       gCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTttgt                                                                                                                               <  2:810377/150‑1 (MQ=255)
                       gCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTttgt                                                                                                                               >  1:778875/1‑150 (MQ=255)
                         tttGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTACAAAATTTGCACCGTAGTAAAGTTttgttg                                                                                                                             <  2:818929/150‑1 (MQ=255)
                          ttGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGc                                                                                                                            <  1:1255704/150‑1 (MQ=255)
                             atatGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGcccc                                                                                                                         <  2:1691576/150‑1 (MQ=255)
                                          cGGCTTTCCGAAGCACCATCCAGAACTCTAGTTCCGTTCCCCGGTCGATTTTTTCATTATCGCCATACCAGATGCGTTCCGTTCCTACCTTTTTCCCACGGAGCAAAAATTGCAACGTAGTAAAGTGTTGATGCCCCCAACGCAGTAAta                                                                                                            >  1:1539459/1‑149 (MQ=255)
                                           ggCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTATTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTTAAGTTTTGTTGCCCCCAACGCAGTAAttt                                                                                                           >  2:946477/1‑150 (MQ=255)
                                             cTTTCTGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTc                                                                                                         <  1:121168/150‑1 (MQ=255)
                                              tttCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCt                                                                                                        <  2:1298246/150‑1 (MQ=255)
                                               ttCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCtt                                                                                                       <  2:332055/150‑1 (MQ=255)
                                                tCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTg                                                                                                      <  1:1651578/150‑1 (MQ=255)
                                                   gTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCATGCct                                                                                                   >  2:230237/1‑150 (MQ=255)
                                                    tAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGTTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCctc                                                                                                  <  2:666978/150‑1 (MQ=255)
                                                      gCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCtt                                                                                                >  1:487298/1‑150 (MQ=255)
                                                        aCCATCCAGAACTCTTGTTCCGTTCCCATGTCGATATTTTCTTTATCGACATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGGATAGTTTTGTTTCCCCCAACGCAGTAATTATCATGAATCttac                                                                                              >  2:89691/1‑148 (MQ=255)
                                                        aCCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTcc                                                                                              >  2:319392/1‑150 (MQ=255)
                                                        aCCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTcc                                                                                              <  1:1109667/150‑1 (MQ=255)
                                                           aTCCAGAACTCTTGTACCGTTCCCAGGGCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCa                                                                                           <  2:10669/150‑1 (MQ=255)
                                                            tCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCAc                                                                                          >  2:1460951/1‑150 (MQ=255)
                                                             ccAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACg                                                                                         <  2:812346/150‑1 (MQ=255)
                                                              cAGAACTCTTGTTCCGTTCCCAGGTCGATATATTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTAGCACCCTAGAAAAGATTTCTTGCCCCCAACGCAGTAATTTTCTAGCCTCTTCCGCACgc                                                                                        >  2:1360045/1‑149 (MQ=255)
                                                               aGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCACCAACGCAGTAATTTTCTTGCCTCTTCCTCACGAc                                                                                       >  1:163014/1‑150 (MQ=255)
                                                                      ttGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc                                                                                <  1:860898/150‑1 (MQ=255)
                                                                        gttccgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCAc                                                                              <  1:951013/150‑1 (MQ=255)
                                                                          tccgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTg                                                                            <  1:386463/150‑1 (MQ=255)
                                                                            cgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCACAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTc                                                                          <  2:760826/150‑1 (MQ=255)
                                                                            cgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGATCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTc                                                                          <  2:505149/150‑1 (MQ=255)
                                                                                cccAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGcc                                                                      >  2:1454482/1‑150 (MQ=255)
                                                                                   aGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGccccc                                                                   <  2:476704/150‑1 (MQ=255)
                                                                                    ggTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCa                                                                  <  2:537429/150‑1 (MQ=255)
                                                                                    ggTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCa                                                                  >  2:109002/1‑150 (MQ=255)
                                                                                       cGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTa                                                               >  2:648600/1‑150 (MQ=255)
                                                                                       cGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTa                                                               >  2:1682723/1‑150 (MQ=255)
                                                                                        gATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACATTTTGCACTGTCAGCCCCCATTAc                                                              >  2:1624367/1‑150 (MQ=255)
                                                                                        gATATTTTCTTTATCGCCATACCAGATGCGGTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCCCCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAAATTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCCGCCCCCATTAc                                                              <  1:1726800/150‑1 (MQ=255)
                                                                                           atTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCAc                                                           <  2:487298/150‑1 (MQ=255)
                                                                                                  ttATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATg                                                    <  2:163014/150‑1 (MQ=255)
                                                                                                  ttATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCACACGGTTCAAAATTTGCACCGAAGTAAAGTTTTGTTGCACCCAACGAAGTAATTTTCTTGCATCTTCCTCACGAACTGTTTCACTTGCAGCCCCCATTACCACTGCAATg                                                    >  1:80950/1‑150 (MQ=255)
                                                                                                   tATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAAtt                                                                                                                                                    <  1:299021/53‑1 (MQ=255)
                                                                                                   tATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAAtt                                                                                                                                                    >  2:299021/1‑53 (MQ=255)
                                                                                                   tATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATga                                                   >  1:551453/1‑150 (MQ=255)
                                                                                                     tCGCCATACCCGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATgaga                                                 <  2:1688432/150‑1 (MQ=255)
                                                                                                       gCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCCGCCCCCATTACCACTGCAATGAgacg                                               >  1:35187/1‑150 (MQ=255)
                                                                                                         cATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAgacgac                                             <  2:700211/150‑1 (MQ=255)
                                                                                                         cATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAgacgac                                             <  2:1221329/150‑1 (MQ=255)
                                                                                                           tACCAGATGAGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGc                                           <  2:941329/150‑1 (MQ=255)
                                                                                                               tgATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCTAAATTTGCACCGTAGTATAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCGTCTTCCTCACGTCCTTTTGCACTGTCAGCCCCCATTACCACTGCATTGAGACGACGCTGcc                                       <  2:573711/149‑1 (MQ=255)
                                                                                                                  tGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCAt                                    >  1:686466/1‑150 (MQ=255)
                                                                                                                    cgttccgttcCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCt                                  >  1:1258547/1‑150 (MQ=255)
                                                                                                                     gttccgttccGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTa                                 >  2:1727363/1‑150 (MQ=255)
                                                                                                                      ttccgttccgACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTAc                                <  1:667685/150‑1 (MQ=255)
                                                                                                                          gttccgACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGCCCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGATGCTGCCGATCTACAGcc                            >  1:1208994/1‑150 (MQ=255)
                                                                                                                          gttccgACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTAGTCCTCACGCCCTTATGCACTGTCAGCCACCATTACCACTGGAATGAGCCGAAGATGCGCTTGTACTGcc                            >  1:76199/1‑150 (MQ=255)
                                                                                                                              cgACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAg                        <  2:1546083/150‑1 (MQ=255)
                                                                                                                               gACCTTTTTACCAAGGTGAAAAATTTTCACCGTTGTAAAGTTTTGTTGCACCAAACTCAGTAATTTTCTTGCCTCTTCATCACGAACTTTTGCACAGTCAGCCCCGATTACAACTTCAATGAGACTACGCTGCCCATCTACAGCCTAAGc                       <  2:1173351/150‑1 (MQ=255)
                                                                                                                                aCCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCa                      <  1:1290996/150‑1 (MQ=255)
                                                                                                                                   attttCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATg                   <  1:134900/149‑1 (MQ=255)
                                                                                                                                    ttatCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCCTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATga                  <  1:690279/147‑1 (MQ=255)
                                                                                                                                     tttCCCTCGGTGCAAAATTTTCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTAACACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATgag                 <  1:165653/150‑1 (MQ=255)
                                                                                                                                        cccACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGAtt              >  1:3359/1‑150 (MQ=255)
                                                                                                                                         ctaCGGTGCAAATTTTGCACCGTGGTAAAGTTTTGTTGCCCCCAACCCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTa             >  2:733277/3‑150 (MQ=255)
                                                                                                                                         ctaCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTa             >  2:1704366/3‑150 (MQ=255)
                                                                                                                                         ctaCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTa             >  2:1519867/3‑150 (MQ=255)
                                                                                                                                         ctaCGGTGCAAATTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTa             >  2:468177/3‑150 (MQ=255)
                                                                                                                                            cGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTAGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAAc          <  2:73393/150‑1 (MQ=255)
                                                                                                                                                gCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCgg      >  1:1657035/1‑150 (MQ=255)
                                                                                                                                                  aaaaTTTGCAGCGTATTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGAAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCa    <  1:1603465/150‑1 (MQ=255)
                                                                                                                                                  aaaaTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGCCGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCa    >  2:626433/1‑150 (MQ=255)
                                                                                                                                                    aaTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCAcc  <  2:1533750/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CTTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACC  >  NC_000913/2081539‑2081836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: