Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,115,847 G→T 6.0% A17E (GCG→GAG)  wcaM ← colanic acid biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,115,8470GT 6.0% 278.8 / 4.2 102A17E (GCG→GAG) wcaMcolanic acid biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base G (42/53);  new base T (3/4);  total (45/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

AACCAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGAC  >  NC_000913/2115699‑2115996
                                                                                                                                                     |                                                                                                                                                     
aaCCAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAgcgc                                                                                                                                                      >  2:154804/1‑150 (MQ=255)
aaCCAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGGGGGTTGTTGTCGTTAATGTTTACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAgcgc                                                                                                                                                      >  1:566481/1‑150 (MQ=255)
  ccAATCCGGCAGGCACGACGACTGTTTGCCCATCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGGGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCg                                                                                                                                                    <  1:650996/150‑1 (MQ=255)
  ccAATCCGGCAGGCACGACGACTGTGTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCg                                                                                                                                                    <  1:643940/150‑1 (MQ=255)
   cAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGGGGGCAGCGCGCGGGGGAAAGGGGTTTGGGGGAAGGCAAGCGCCgc                                                                                                                                                   >  2:1573008/1‑149 (MQ=255)
    aaTCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAg                                                                                                                                                  >  1:1186823/1‑150 (MQ=255)
           caggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGGCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCg                                                                                                                                           <  1:664659/150‑1 (MQ=255)
            aggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCTCGGCGCTTGcctt                                                                                                                                          <  1:516643/150‑3 (MQ=255)
             ggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTTGTCGTTAATGTTTACGCGTTGGCGGGCGGGAAGCGCGCGGGCGAAAGGGGTTTGGAGGAAGGCAAGCGCCGAGCTTGCCggc                                                                                                                                         >  2:1522633/1‑148 (MQ=255)
              gcaCGACGCGTGGTTGCCGTTCGCTGAAGGCTGGTTTATATGAGGCGATCCTGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGATGCGCGCGGGCGAAATGGGTATGCAGGAAGGCAAGCGCCGAGCTTGCCGTCa                                                                                                                                        <  2:945357/150‑1 (MQ=255)
              gcaCGACGACTGTTGGCCCTGCGCTGAAGGCTTGTTTAACTGAGGCGATCCAGTCGGGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCTCGGCGCTTGCCTTcc                                                                                                                                        <  1:1083322/150‑2 (MQ=255)
               caCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGATGAAGGCAAGCGCCGAGCTTGCCGTCAg                                                                                                                                       >  2:155423/1‑150 (MQ=255)
               caCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCTCGGCGCTTGCCTTcct                                                                                                                                       <  1:1127174/150‑3 (MQ=255)
               caCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGCTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGGGGAAGGCAAGCGCCGAGCTTGCCGTCAg                                                                                                                                       <  1:1230596/150‑1 (MQ=255)
                acgacgACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTTATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGGGAAAGGGGGATTGTGGAAGGCAAGCGCCGAGCATGCCGTCAgg                                                                                                                                      >  1:729089/1‑150 (MQ=255)
                  gacgacTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATTTTAACGCTTTGTCGGGCGGGAACCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGCGCATGCCGTCAGGaa                                                                                                                                    >  1:489842/1‑150 (MQ=255)
                  gacgacTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTTACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGaa                                                                                                                                    >  2:143861/1‑150 (MQ=255)
                   acgacTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAAAGATTTTTAGGGCCGGAAGCGCGCGGGCGAAAGGGGTATGGTGGAAGGCAAGAGCCGAGCTTGCCGTCAGGAAc                                                                                                                                   >  1:530726/1‑150 (MQ=255)
                     gacTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGt                                                                                                                                 >  1:194517/1‑150 (MQ=255)
                      gcTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGACCGTg                                                                                                                                >  1:1243960/2‑150 (MQ=255)
                         gTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCgg                                                                                                                             >  2:519428/1‑149 (MQ=255)
                                cTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTTGTCGTTAATTTTAACGCTTTTTCGGGCGGGTATCGCGCGGGCGAAAGGGGTTTGGAGGAAGGCAAGCGCCGAGCTTTGCGTCAGGAACGTGCGTCGGgagt                                                                                                                      >  1:1349966/1‑149 (MQ=255)
                                  tCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCTCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGt                                                                                                                    <  2:1634712/150‑1 (MQ=255)
                                    gCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGttt                                                                                                                  <  2:1004610/150‑1 (MQ=255)
                                      tGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGttttt                                                                                                                <  2:504742/150‑1 (MQ=255)
                                       gAAGGCTTGTTTAAATGAGGCGATCCCGTCGTGTGGGTGGTAGTCATTAATGTTAACGCTTTGTCGGGCGGTAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGtttttt                                                                                                               <  2:1300486/150‑1 (MQ=255)
                                        aaGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTa                                                                                                              <  2:1498094/150‑1 (MQ=255)
                                        aaGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTa                                                                                                              <  2:44680/150‑1 (MQ=255)
                                        aaGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTa                                                                                                              <  1:361672/150‑1 (MQ=255)
                                         cggCTTGTATAAATGCGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTaa                                                                                                             <  2:1584965/149‑1 (MQ=255)
                                          ggCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTaaa                                                                                                            <  1:612017/150‑1 (MQ=255)
                                          ggCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTaaa                                                                                                            >  2:520954/1‑150 (MQ=255)
                                            cgtGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATg                                                                                                          <  2:14924/148‑1 (MQ=255)
                                            cTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATTTTAACCCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTTTGGAGGAAGGCAAGCGCCCAGCGTGCCGTCAGGCACGTGCGTCGGGAGAGTTTTTTTAATg                                                                                                          >  2:1381652/1‑150 (MQ=255)
                                               gTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTATTAGTTAATGTTTACGGTTTGTCGGGCGGGAAGCGCGCGGGCGAAATGGGTATTGAGGAAGGCAAGCTCCGAGCTTGCCGTCAGGAACGTGCGTCGGGTGAGTTTTTTAAATGGCa                                                                                                       >  1:804962/1‑150 (MQ=255)
                                                   aactGAGGCGATCCAGTCGTGTGGGGTGTAGTCGCTAATGTTAACGCCTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCg                                                                                                   <  1:1404785/147‑1 (MQ=255)
                                                    aaTGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAATGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGt                                                                                                  <  1:1107830/150‑1 (MQ=255)
                                                     aTGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGtt                                                                                                 >  1:274038/1‑150 (MQ=255)
                                                      tGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTtc                                                                                                >  2:109286/1‑150 (MQ=255)
                                                      tGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTtc                                                                                                <  1:1473873/150‑1 (MQ=255)
                                                      tGAGGCGAGCCAGTCGTGTGGGGTGTAGTCGTTAATGTTTACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGGATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTtc                                                                                                <  1:1664740/150‑1 (MQ=255)
                                                        aGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAAAGCTTTGTCGGGCGGGCAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTTCGTCGGGAGAGTTTTTTAAATGGCATGCGTtctc                                                                                              >  1:1484325/1‑150 (MQ=255)
                                                         ggCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCa                                                                                                       <  1:1014261/140‑1 (MQ=255)
                                                         ggCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCa                                                                                                       >  2:1014261/1‑140 (MQ=255)
                                                         ggCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGCTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTctcc                                                                                             <  2:1239363/150‑1 (MQ=255)
                                                           cGATCCACTCGTGTTGGTTGTAGGCGTAAATGTTATCGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTctcctc                                                                                           <  2:1313439/150‑1 (MQ=255)
                                                             aTCCAGTCGTGGTGGTTGTAGTCGTTAATGTTTACGCGTTGTCGGGGGGGAAGCGCGGGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGCGCTTGCCGTCCGGAACGTGCGTCGGGAGTGTTTTTTTAATGGCATGCGTTgtcctcgt                                                                                         >  2:797406/1‑148 (MQ=255)
                                                               ccAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGGCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCtata                                                                                       >  2:104066/1‑150 (MQ=255)
                                                                  gTCGTGTGGGTTGTAGGCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAg                                                                                    <  2:1358690/150‑1 (MQ=255)
                                                                   gcgggggggTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGc                                                                                   <  1:1511982/144‑1 (MQ=255)
                                                                    cGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGcc                                                                                  <  2:1194691/150‑1 (MQ=255)
                                                                          ggTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgcagc                                                                            <  2:1330066/150‑1 (MQ=255)
                                                                          ggTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGACAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCAAGCGTTCTCCTCTATAAATCCTgcagc                                                                            >  1:77119/1‑150 (MQ=255)
                                                                             tGTAGTCGATAATGATAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCaa                                                                          <  2:952791/150‑1 (MQ=255)
                                                                                   cGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGcgtaat                                                                   >  2:612449/1‑145 (MQ=255)
                                                                                   cGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCg                                                                   >  2:784021/1‑150 (MQ=255)
                                                                                   cGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCg                                                                   <  2:274038/150‑1 (MQ=255)
                                                                                   cGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCg                                                                   >  1:105103/1‑150 (MQ=255)
                                                                                   cGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCg                                                                   >  1:855339/1‑150 (MQ=255)
                                                                                     ttAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAg                                                                 <  2:1193695/150‑1 (MQ=255)
                                                                                     ttAATGGTAACGCTATGGCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGCGGAATGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCTGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATCAAGCCTGCAGCAAGCTGGCGAg                                                                 <  2:176608/150‑1 (MQ=255)
                                                                                      tAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGt                                                                >  1:899069/1‑150 (MQ=255)
                                                                                          gTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTtctc                                                            >  1:623872/1‑150 (MQ=255)
                                                                                          gTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTtctc                                                            >  1:676220/1‑150 (MQ=255)
                                                                                          gTTAACGCATTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTGCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTtctc                                                            <  1:1621457/150‑1 (MQ=255)
                                                                                           ttAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCg                                                           <  1:909019/150‑1 (MQ=255)
                                                                                            tAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGa                                                          <  2:738679/150‑1 (MQ=255)
                                                                                             aaCGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGAt                                                         <  1:11127/150‑1 (MQ=255)
                                                                                             aaCGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGAt                                                         >  2:342260/1‑150 (MQ=255)
                                                                                             aaCGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGAt                                                         >  2:1679405/1‑150 (MQ=255)
                                                                                              aCGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGAtt                                                        <  2:437109/150‑1 (MQ=255)
                                                                                               cGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTg                                                       <  1:573966/150‑1 (MQ=255)
                                                                                                 cTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGAt                                                     <  2:1271427/150‑1 (MQ=255)
                                                                                                  tttGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATc                                                    <  1:154804/150‑1 (MQ=255)
                                                                                                  ttagTCGGGCTGGAAGCGCGCGGGCGAAAGGGGTATGGATGAAGTCAAGCGCCGAGCTTGGCGTCAGGTACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATc                                                    <  1:762800/147‑1 (MQ=255)
                                                                                                     gTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCAcc                                                 >  2:1232526/1‑150 (MQ=255)
                                                                                                        gggcgggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctgc                                              <  2:1415694/150‑1 (MQ=255)
                                                                                                          gcgggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctgctg                                            <  1:993964/150‑1 (MQ=255)
                                                                                                          gcgggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctgctg                                            <  2:1186823/150‑1 (MQ=255)
                                                                                                             ggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGtt                                         >  2:1254134/1‑150 (MQ=255)
                                                                                                                    cgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCg                                  <  1:109286/150‑1 (MQ=255)
                                                                                                                     gcgGGCGAAAGGGGTATGGTGGAAGGCAAGCGGCGAGCTTGCCGTCAGGATCGTTCGTCGGGTGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATAgt                                 <  1:511675/150‑1 (MQ=255)
                                                                                                                     gcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCgt                                 >  2:1477188/1‑150 (MQ=255)
                                                                                                                      cgGGCTAAAGGGGTATGGAGGAATGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCgtg                                <  1:11977/150‑1 (MQ=255)
                                                                                                                      cgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCgtg                                <  2:167526/150‑1 (MQ=255)
                                                                                                                      cgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCgtg                                <  2:566481/150‑1 (MQ=255)
                                                                                                                      cgGGCGAAAGGGGTATGGAGCAAGGCTAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCgtg                                <  2:157024/150‑1 (MQ=255)
                                                                                                                           gAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCaa                           >  1:763464/1‑150 (MQ=255)
                                                                                                                                     aTGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGGTCAACTTTTTcgc                  >  2:71255/1‑150 (MQ=255)
                                                                                                                                      tGGAGGAAGGCAAGCGCCGTGCGTGCCGTCAGGACCGTGCGGCGGGAGAGTTATTTTCTTTGCATGCGTTCTCCTCTATAAAGCCTGGAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcg                 <  2:875259/150‑1 (MQ=255)
                                                                                                                                        gaggaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgagcg               >  2:14943/1‑150 (MQ=255)
                                                                                                                                         ctgacggCAAGCTCGGCGCTTGCCTTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgc              >  1:745769/6‑150 (MQ=255)
                                                                                                                                         aggaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgc              >  2:399720/1‑150 (MQ=255)
                                                                                                                                          tgacggCAAGCTCGGCGCTTGCCTTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCGATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgcg             >  2:1458212/5‑150 (MQ=255)
                                                                                                                                             aGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGt          >  2:402967/1‑150 (MQ=255)
                                                                                                                                             aGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGt          >  2:1294815/1‑150 (MQ=255)
                                                                                                                                              ggCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATGACCTGCTGGTTTAAATCGTGTTCTACTTTTTCGCGCGCGCGtt         <  2:487582/150‑1 (MQ=255)
                                                                                                                                                cAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTg       <  2:676220/150‑1 (MQ=255)
                                                                                                                                                  aGCGGCGAGCTCGCCGTCAGGACCGTGCGTCGGTTGAGTTTTTTAAATGGCATGCGTTCTCCTCTTTAAAGCCTGCAGCACGCTGGCGAGTTCTCGAGTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTgtc     <  1:204858/150‑3 (MQ=255)
                                                                                                                                                     gcCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGAGTTCAACTTTTTCGCGCGCGCGTTTgacgac  >  1:259228/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AACCAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGAC  >  NC_000913/2115699‑2115996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: