Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,126,159 A→T 7.2% F22I (TTT→ATT) ‡ wcaH ← GDP‑mannose mannosyl hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,126,1590AT 7.2% 248.6 / 7.0 92F22I (TTT→ATT) ‡wcaHGDP‑mannose mannosyl hydrolase
Reads supporting (aligned to +/- strand):  ref base A (31/54);  new base T (2/5);  total (33/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.29e-01

CCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCGTGATACC  >  NC_000913/2126011‑2126307
                                                                                                                                                    |                                                                                                                                                    
cccAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCGGAAACTCGCCGCGACTGTTCTCGACAATAAAg                                                                                                                                                     >  2:114035/1‑150 (MQ=255)
cccAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATaaat                                                                                                                                                     >  2:130880/1‑149 (MQ=255)
cccAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAg                                                                                                                                                     >  1:1370842/1‑150 (MQ=255)
  cAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTc                                                                                                                                                   <  1:1298902/150‑1 (MQ=255)
   aGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCg                                                                                                                                                  <  2:394566/150‑1 (MQ=255)
   aGTTCCGCCATCGGCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCg                                                                                                                                                  <  1:130056/150‑1 (MQ=255)
     ttCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACTTTATTGTCgag                                                                                                                                                <  1:1594695/150‑1 (MQ=255)
     ttCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCgag                                                                                                                                                <  1:245466/150‑1 (MQ=255)
     gtCCGCCAGCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGGTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCgag                                                                                                                                                <  2:700576/149‑1 (MQ=255)
        cGCCCTCGGCACCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTATTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACTTTATTGTCgagaac                                                                                                                                             <  1:72887/150‑3 (MQ=255)
        cGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACTTTATTGTCgagaac                                                                                                                                             <  2:916919/150‑3 (MQ=255)
        cGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCgagaga                                                                                                                                             <  1:1730290/150‑1 (MQ=255)
         gCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCgagagag                                                                                                                                            >  1:493473/1‑150 (MQ=255)
         gCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGCCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACTTTATTGTCgagaaca                                                                                                                                            <  1:172408/150‑4 (MQ=255)
          ccATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGAGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCgagagaga                                                                                                                                           <  1:1179001/150‑1 (MQ=255)
           cATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGAc                                                                                                                                          >  2:650246/1‑150 (MQ=255)
           cATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGCTCTCGACTTTATTGTCgagaacagt                                                                                                                                          <  1:1313222/150‑6 (MQ=255)
            aTCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGGTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGCCTGTTCTCGACAATAAAGTCGAGAGAGACa                                                                                                                                         >  2:493270/1‑150 (MQ=255)
             tCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACaa                                                                                                                                        >  2:1145139/1‑150 (MQ=255)
              cgacAGCCGCTCAAATGCGGCTTCCAGCGTTTCGGCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGCCGAGAGAGACAAg                                                                                                                                       <  1:83454/147‑1 (MQ=255)
              cGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAg                                                                                                                                       <  2:63344/150‑1 (MQ=255)
              cGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAg                                                                                                                                       <  2:290158/150‑1 (MQ=255)
                gaaGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCATTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGGCGAGAGAGACAAGCg                                                                                                                                     <  2:1237870/148‑1 (MQ=255)
                 cAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCgg                                                                                                                                    <  2:981751/150‑1 (MQ=255)
                 cAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCgg                                                                                                                                    >  2:741589/1‑150 (MQ=255)
                   gCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAg                                                                                                                                  <  2:988579/150‑1 (MQ=255)
                   cccGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAg                                                                                                                                  <  1:1186855/149‑1 (MQ=255)
                        tCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAg                                                                                                                             <  2:1370842/150‑1 (MQ=255)
                         cAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAgc                                                                                                                            >  2:586982/1‑150 (MQ=255)
                           aaTGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGGTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCTGAGTGGAgcgc                                                                                                                          >  2:93510/1‑150 (MQ=255)
                              gCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACt                                                                                                                       <  2:510946/150‑1 (MQ=255)
                               cGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTa                                                                                                                      >  2:1199913/1‑150 (MQ=255)
                                ggCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTAc                                                                                                                     <  2:975/150‑1 (MQ=255)
                                     ccAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTgg                                                                                                                <  1:913046/150‑1 (MQ=255)
                                     ccAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTgg                                                                                                                <  2:532177/150‑1 (MQ=255)
                                     ccAGCGGTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGCTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGGCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTgg                                                                                                                <  1:91093/150‑1 (MQ=255)
                                          gTTTCGTCTTTCTACACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAg                                                                                                           <  1:1718200/150‑1 (MQ=255)
                                                tCTTTCTGCACTCGCCCTCCCGGCCCACACCAGTAAGCCTGCGCCGGGCGGTTGGGTCTTTTGCCAAGCAGACACTCGCGGCGACTGATCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTcc                                                                                                     <  1:116129/150‑1 (MQ=255)
                                                tCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTATTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTcc                                                                                                     <  1:19193/150‑1 (MQ=255)
                                                gcTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTcc                                                                                                     <  1:1500869/149‑1 (MQ=255)
                                                    tCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTgcc                                                                                                 >  2:663772/1‑148 (MQ=255)
                                                    tCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGAc                                                                                                 >  2:1441750/1‑150 (MQ=255)
                                                            cgcCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAAc                                                                                         >  1:1210305/1‑150 (MQ=255)
                                                                 tCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCaaa                                                                                                            <  1:361710/126‑1 (MQ=255)
                                                                 tCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCaaa                                                                                                            >  2:361710/1‑126 (MQ=255)
                                                                  cccGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCAtt                                                                                   >  1:389330/1‑150 (MQ=255)
                                                                         caAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCg                                                                            >  2:369990/1‑150 (MQ=255)
                                                                          aaaCCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGa                                                                           <  2:376834/150‑1 (MQ=255)
                                                                           aaCCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGaa                                                                          >  2:1638851/1‑150 (MQ=255)
                                                                            aCCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGaaa                                                                         >  2:358554/1‑150 (MQ=255)
                                                                            aCCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGaaa                                                                         >  1:982920/1‑150 (MQ=255)
                                                                             ccAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAg                                                                        >  1:489400/1‑150 (MQ=255)
                                                                                gTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCgg                                                                     <  1:103025/150‑1 (MQ=255)
                                                                                 tAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGt                                                                    <  1:184005/150‑1 (MQ=255)
                                                                                  aaCCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTc                                                                   >  2:869537/1‑150 (MQ=255)
                                                                                  aaCCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGAAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGAAAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATAATTACCCCCGAAAGAGGTc                                                                   >  1:351705/1‑150 (MQ=255)
                                                                                   aCCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCTCCGCGACTGTTCTCGACAATATATGCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCt                                                                  <  1:291899/150‑1 (MQ=255)
                                                                                   aCCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCt                                                                  <  1:869537/150‑1 (MQ=255)
                                                                                   aCCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCt                                                                  <  2:493473/150‑1 (MQ=255)
                                                                                     ccTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTg                                                                <  2:1195124/150‑1 (MQ=255)
                                                                                       tGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTGGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGAt                                                              <  1:140062/150‑1 (MQ=255)
                                                                                       tGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGAt                                                              <  2:378251/150‑1 (MQ=255)
                                                                                       tGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGAt                                                              <  2:398659/150‑1 (MQ=255)
                                                                                          gcCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATtct                                                           <  2:1508270/150‑1 (MQ=255)
                                                                                           ccGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATtctc                                                          <  1:741589/150‑1 (MQ=255)
                                                                                            cGGGCGGTTGGTTCTTTTGCAAATCATAAACTCGCCGCGACTGTTCTAGACTATAAAGTAGAGAGAGACAAGCGGAGTGGAGCGCTCTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGATAGCGGTCTTGATTCTCa                                                         <  2:1563443/150‑1 (MQ=255)
                                                                                             gggCGGTTGGGTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCaa                                                        <  2:983752/150‑1 (MQ=255)
                                                                                               gCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAgg                                                      >  1:1178885/1‑150 (MQ=255)
                                                                                                  gttggttCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGCAc                                                   >  2:332210/1‑150 (MQ=255)
                                                                                                  gttggttCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAAc                                                   <  1:1618353/150‑1 (MQ=255)
                                                                                                        tCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTgg                                             >  1:902166/1‑150 (MQ=255)
                                                                                                          ttttGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTa                                           <  2:1627442/150‑1 (MQ=255)
                                                                                                            ttGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTTGTAAg                                         <  1:655037/150‑1 (MQ=255)
                                                                                                              gCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTg                                       >  1:1284909/1‑150 (MQ=255)
                                                                                                                cAAGCAGAAACTCGCCGAGACTGTTCTCTCCAATAAAGTCGAGAGAGACAAGCCGAGTGGAGCGCACTACCGTGGCAAAGTCTACCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCt                                     <  2:1551168/150‑1 (MQ=255)
                                                                                                                   gCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACACGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGc                                  >  1:1053470/1‑150 (MQ=255)
                                                                                                                     aGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCaa                                <  2:533769/150‑1 (MQ=255)
                                                                                                                            cgccgcGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTATTGATTCTCAAGGAACCACTGGTAAGTGCTGGAAAGCCCCGc                         >  2:1273461/1‑150 (MQ=255)
                                                                                                                             gccgcGACTGTTCACGACAAAAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCt                        <  2:1551043/150‑1 (MQ=255)
                                                                                                                              ccgcGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCtt                       >  2:799089/1‑150 (MQ=255)
                                                                                                                                gcgACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTcc                     <  1:1617156/150‑1 (MQ=255)
                                                                                                                                    cTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTg                 <  1:891698/150‑1 (MQ=255)
                                                                                                                                     tGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTga                <  1:1019815/150‑1 (MQ=255)
                                                                                                                                     tGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACCTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTga                <  1:18033/150‑1 (MQ=255)
                                                                                                                                     ctctctcGACTTTATTGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTga                >  2:1619190/4‑150 (MQ=255)
                                                                                                                                      gTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTgag               <  1:1638851/150‑1 (MQ=255)
                                                                                                                                      gTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTgag               <  1:650246/150‑1 (MQ=255)
                                                                                                                                      gTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTgag               >  1:1412667/1‑150 (MQ=255)
                                                                                                                                       ttCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGc                                  >  1:1476875/1‑130 (MQ=255)
                                                                                                                                       ttCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGc                                  <  2:1476875/130‑1 (MQ=255)
                                                                                                                                       ctctcGACTTTATTGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTgaga              >  2:908208/2‑150 (MQ=255)
                                                                                                                                            gACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCg         <  1:577782/150‑1 (MQ=255)
                                                                                                                                              cAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCGTg       <  2:1210305/150‑1 (MQ=255)
                                                                                                                                                   aaGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTGCCAGTGAGATTTCGTGATAcc  >  1:505019/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCGTGATACC  >  NC_000913/2126011‑2126307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: