Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,133,447 A→C 6.2% intergenic (‑50/+43) wcaA ← / ← wzc putative glycosyl transferase/colanic acid production tyrosine‑protein kinase; autokinase; Ugd phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,133,4470AC 6.2% 231.0 / 5.2 81intergenic (‑50/+43)wcaA/wzcputative glycosyl transferase/colanic acid production tyrosine‑protein kinase; autokinase; Ugd phosphorylase
Reads supporting (aligned to +/- strand):  ref base A (42/34);  new base C (2/3);  total (44/37)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01

TGCTGTAGTCCTGGCGCAAAACCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAATACCG  >  NC_000913/2133298‑2133595
                                                                                                                                                     |                                                                                                                                                    
tGCTGTAGTCCTGGCGCAAAACCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGa                                                                                                                                                      <  1:1111535/150‑1 (MQ=255)
 gCTGTAGTCCTGGCGCAAAACCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGAGGCGGCGTGaa                                                                                                                                                     >  2:1506652/1‑150 (MQ=255)
   tGTAGTCCTGGCGCAAAACCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACg                                                                                                                                                   >  1:489865/1‑150 (MQ=255)
   tGTAGTCCTGGCGCAAAACCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACg                                                                                                                                                   <  1:432821/150‑1 (MQ=255)
   tGTAGTCCTGGCGCAAAACCGATTTTATTCCCCGAATCGCAAGTTGTTTGCTGTTCCAGGTCGGCATATAGATTGAGATCATCGGATTGTTATTCATAGGTTGTTCTCCCGCCTTATGAGCTCGTTTGATTGCCGGATGCGGCGTTAACg                                                                                                                                                   >  2:343426/1‑150 (MQ=255)
     tAGTCCTGGCGCAAAACCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGcc                                                                                                                                                 <  1:636330/150‑1 (MQ=255)
     tAGTCCTGGCGCAAAACCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGcc                                                                                                                                                 <  1:1466848/150‑1 (MQ=255)
     tAGTCCTGGCGCAAAACCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGCTCGGCATATAGATTGAGCTCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGcc                                                                                                                                                 >  1:1611763/1‑150 (MQ=255)
      aGTCCTGGCGCAAAACCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCt                                                                                                                                                >  1:16913/1‑150 (MQ=255)
       gTCCTGGCGCAAAACCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCtt                                                                                                                                               >  2:1707349/1‑150 (MQ=255)
         ccTGGCGCAAAACCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                                                             >  1:958362/1‑150 (MQ=255)
           tGGCGCAAAACCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATcc                                                                                                                                           >  2:1332580/1‑150 (MQ=255)
           tGGCGCAAAACCGATTTTATTGCCCGAATCGCCAGGTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATcc                                                                                                                                           >  1:1112849/1‑150 (MQ=255)
               gcAAAACCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                                       <  2:195305/150‑1 (MQ=255)
                 aaaaCCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGGTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATAAGGCGTTCACGCCGCATCCGGcaag                                                                                                                                     <  1:1607724/150‑4 (MQ=255)
                  aaaCCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATAAGGCGTTCACGCCGCATCCGGcaagc                                                                                                                                    <  1:24365/150‑5 (MQ=255)
                    aCCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCATGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCACGGCTTATGAGCTGGTTTGCTTGCCGGTTGCGGCGTGAACGCCTTATCCGTCCTACgg                                                                                                                                  >  1:628401/1‑150 (MQ=255)
                    aCCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGGTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATAAGGCGTGCACGCCGCATCCGGcaagcaa                                                                                                                                  <  1:839310/150‑7 (MQ=255)
                      cGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACgggg                                                                                                                                <  2:1684177/150‑1 (MQ=255)
                           ttATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTGCGGGGCGGTg                                                                                                                           >  1:920840/1‑150 (MQ=255)
                           ttATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATACGTTGATCTCCAGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTCCGGGGCGGTg                                                                                                                           >  2:1136960/1‑150 (MQ=255)
                                gCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGTATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAAt                                                                                                                      >  1:1167467/1‑150 (MQ=255)
                                gCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAAt                                                                                                                      >  1:729906/1‑150 (MQ=255)
                                     aaTCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGCGATCAGCGGAGTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAgg                                                                                                                 <  1:944757/150‑1 (MQ=255)
                                      aTCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGc                                                                                                                <  1:1182184/150‑1 (MQ=255)
                                        cGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAACTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGcgt                                                                                                              <  1:1587631/150‑1 (MQ=255)
                                        cGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAACTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGcgt                                                                                                              <  1:282849/150‑1 (MQ=255)
                                         gCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGcgtc                                                                                                             >  1:899191/1‑150 (MQ=255)
                                            aGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGtt                                                                                                          <  2:715866/150‑1 (MQ=255)
                                              ttgttgGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATCGGGTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTAt                                                                                                        <  1:1597786/150‑1 (MQ=255)
                                              gtgttgGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTAt                                                                                                        <  1:1447466/149‑1 (MQ=255)
                                               tgttgGCGGTTCCTGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGTAGGCGTCGTTAtt                                                                                                       <  2:216258/150‑1 (MQ=255)
                                               tgctgGCTGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATAGTTATTCATAGGTTGTTCTCCCGGCTTAAGAGCAGGTTTGCTTGCCGGATGCGGCGAGAACGCCTTATCCGGCATACGGGGCGGAGCGAATGCAGGCGTCGATAtt                                                                                                       >  2:819570/4‑150 (MQ=255)
                                                   ggCGGTTACAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTAGCCTGAAGCGGCGTGAACGACTTTTCCGGCCTACGGGGCGGTGCTAATGCAGGCGTCGTTATTTCGc                                                                                                   >  2:1622263/1‑150 (MQ=255)
                                                         tCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGa                                                                                             <  1:558327/150‑1 (MQ=255)
                                                              gTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTtat                                                                                        <  1:1500569/150‑1 (MQ=255)
                                                               tCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTtata                                                                                       <  1:579251/150‑1 (MQ=255)
                                                                 ggtatatAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTAtatt                                                                                     <  2:1167467/147‑1 (MQ=255)
                                                                 ggCATATAGAGTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCGGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTTTCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTAtatt                                                                                     <  1:42222/150‑1 (MQ=255)
                                                                    atatAGATTGAGATCAGCGGATTGATTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTAGAATCCGACTTAtattcgt                                                                                  >  1:1395416/1‑150 (MQ=255)
                                                                       taGATTGAGATCAGCGGATTGTTTTTCATTGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTAtattcgtatt                                                                               <  1:799836/150‑1 (MQ=255)
                                                                        aGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTAtattcgtattc                                                                              >  1:210346/1‑150 (MQ=255)
                                                                         gATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTAtattcgtattcg                                                                             >  1:852853/1‑150 (MQ=255)
                                                                          aTTGAGATCAGCTGATTGTTTTTCATAGGTTGGCCTCCCGGCTTATGAGCGGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATattcgtattcgt                                                                            <  2:719716/150‑1 (MQ=255)
                                                                          aTTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATattcgtattcgt                                                                            <  2:1388966/150‑1 (MQ=255)
                                                                             gagaTCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAAt                                                                         <  2:369127/150‑1 (MQ=255)
                                                                                aTCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGc                                                                      <  1:680970/150‑1 (MQ=255)
                                                                                       gATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATc                                                               >  2:733963/1‑150 (MQ=255)
                                                                                       gATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATc                                                               >  2:770024/1‑150 (MQ=255)
                                                                                          tGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTg                                                            <  2:631895/150‑1 (MQ=255)
                                                                                             ttttCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTATTAGCCATAAGCCTGATa                                                         >  2:1143658/1‑150 (MQ=255)
                                                                                             ttttCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATa                                                         <  1:580273/150‑1 (MQ=255)
                                                                                                 cATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATAcgcg                                                     >  1:526257/1‑150 (MQ=255)
                                                                                                   tAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGTGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCt                                                   <  2:590360/150‑1 (MQ=255)
                                                                                                      gttgttCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGgc                                                >  1:1144034/1‑150 (MQ=255)
                                                                                                       ttgttCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGgcg                                               >  2:1698989/1‑150 (MQ=255)
                                                                                                           tctcCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCgcggc                                           <  2:934948/150‑1 (MQ=255)
                                                                                                            gtcCGGGCTTATTAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATACGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTTTATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCgcggcg                                          <  2:1729324/149‑1 (MQ=255)
                                                                                                            ctcCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCgcggcg                                          >  2:129929/1‑150 (MQ=255)
                                                                                                                 ggCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGa                                     >  1:1233646/1‑150 (MQ=255)
                                                                                                                    ttATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATgg                                  <  1:1332580/150‑1 (MQ=255)
                                                                                                                      aTGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAg                                >  1:94192/1‑150 (MQ=255)
                                                                                                                          gCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCa                            <  1:424014/150‑1 (MQ=255)
                                                                                                                            tGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGa                          >  1:1535576/1‑150 (MQ=255)
                                                                                                                                  gcttgcCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCAcc                    >  2:630076/1‑150 (MQ=255)
                                                                                                                                  gcttgcCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCAcc                    >  2:77797/1‑150 (MQ=255)
                                                                                                                                   cttgcCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCAccc                   <  2:1239582/150‑1 (MQ=255)
                                                                                                                                   cttgcCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCAccc                   >  1:1093723/1‑150 (MQ=255)
                                                                                                                                    aggcCGGATAAGGCGTTCACGCCGCATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTAGTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCGGGCGCGGCGGAAGATGGAGTTCAGAATCAcccc                  >  1:508494/3‑150 (MQ=255)
                                                                                                                                     tgcCGGATGCGGCGTGAACGCCTTCTCCGGCCGACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCAACCGACTTATATTCGTATGCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCt                 <  2:151383/150‑1 (MQ=255)
                                                                                                                                     tgcCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCt                 >  2:1452500/1‑150 (MQ=255)
                                                                                                                                      gcCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCtt                >  1:1472168/1‑150 (MQ=255)
                                                                                                                                         ggATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCa             >  1:620362/1‑150 (MQ=255)
                                                                                                                                         ggATGCGGCGTGAACGACTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCa             >  1:1005902/1‑150 (MQ=255)
                                                                                                                                          gataaggcgTTCACGCCGCATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCAc            >  2:172179/6‑150 (MQ=255)
                                                                                                                                              cggcgTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGTCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGa        >  1:1131287/1‑150 (MQ=255)
                                                                                                                                               ggcgTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGa                                     <  1:828789/120‑1 (MQ=255)
                                                                                                                                               ggcgTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGa                                     >  2:828789/1‑120 (MQ=255)
                                                                                                                                               ggcgTGAACGCCTAATACGGACTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCTCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGATGGCGCGGCGGAAGATGGAGTTCAGAAGCACCCCTTTCACCGCaa       >  1:1663793/1‑150 (MQ=255)
                                                                                                                                                gcgTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAAt      >  1:1531615/1‑150 (MQ=255)
                                                                                                                                                gcgTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAAt      >  1:400328/1‑150 (MQ=255)
                                                                                                                                                    gAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAATACCg  <  1:1641128/150‑1 (MQ=255)
                                                                                                                                                    gAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAATACCg  >  2:1100814/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGCTGTAGTCCTGGCGCAAAACCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAATACCG  >  NC_000913/2133298‑2133595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: