Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,133,453 T→G 6.5% intergenic (‑56/+37) wcaA ← / ← wzc putative glycosyl transferase/colanic acid production tyrosine‑protein kinase; autokinase; Ugd phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,133,4530TG 6.5% 221.6 / 5.6 77intergenic (‑56/+37)wcaA/wzcputative glycosyl transferase/colanic acid production tyrosine‑protein kinase; autokinase; Ugd phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (39/33);  new base G (2/3);  total (41/36)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

AGTCCTGGCGCAAAACCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAATACCGTTTTGC  >  NC_000913/2133304‑2133601
                                                                                                                                                     |                                                                                                                                                    
aGTCCTGGCGCAAAACCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCt                                                                                                                                                      >  1:16913/1‑150 (MQ=255)
 gTCCTGGCGCAAAACCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCtt                                                                                                                                                     >  2:1707349/1‑150 (MQ=255)
   ccTGGCGCAAAACCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                                                                   >  1:958362/1‑150 (MQ=255)
     tGGCGCAAAACCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATcc                                                                                                                                                 >  2:1332580/1‑150 (MQ=255)
     tGGCGCAAAACCGATTTTATTGCCCGAATCGCCAGGTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATcc                                                                                                                                                 >  1:1112849/1‑150 (MQ=255)
         gcAAAACCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                                             <  2:195305/150‑1 (MQ=255)
           aaaaCCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGGTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATAAGGCGTTCACGCCGCATCCGGcaag                                                                                                                                           <  1:1607724/150‑4 (MQ=255)
            aaaCCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATAAGGCGTTCACGCCGCATCCGGcaagc                                                                                                                                          <  1:24365/150‑5 (MQ=255)
              aCCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCATGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCACGGCTTATGAGCTGGTTTGCTTGCCGGTTGCGGCGTGAACGCCTTATCCGTCCTACgg                                                                                                                                        >  1:628401/1‑150 (MQ=255)
              aCCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGGTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATAAGGCGTGCACGCCGCATCCGGcaagcaa                                                                                                                                        <  1:839310/150‑7 (MQ=255)
                cGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACgggg                                                                                                                                      <  2:1684177/150‑1 (MQ=255)
                     ttATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTGCGGGGCGGTg                                                                                                                                 >  1:920840/1‑150 (MQ=255)
                     ttATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATACGTTGATCTCCAGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTCCGGGGCGGTg                                                                                                                                 >  2:1136960/1‑150 (MQ=255)
                          gCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGTATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAAt                                                                                                                            >  1:1167467/1‑150 (MQ=255)
                          gCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAAt                                                                                                                            >  1:729906/1‑150 (MQ=255)
                               aaTCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGCGATCAGCGGAGTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAgg                                                                                                                       <  1:944757/150‑1 (MQ=255)
                                aTCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGc                                                                                                                      <  1:1182184/150‑1 (MQ=255)
                                  cGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAACTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGcgt                                                                                                                    <  1:282849/150‑1 (MQ=255)
                                  cGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAACTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGcgt                                                                                                                    <  1:1587631/150‑1 (MQ=255)
                                   gCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGcgtc                                                                                                                   >  1:899191/1‑150 (MQ=255)
                                      aGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGtt                                                                                                                <  2:715866/150‑1 (MQ=255)
                                        ttgttgGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATCGGGTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTAt                                                                                                              <  1:1597786/150‑1 (MQ=255)
                                        gtgttgGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTAt                                                                                                              <  1:1447466/149‑1 (MQ=255)
                                         tgttgGCGGTTCCTGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGTAGGCGTCGTTAtt                                                                                                             <  2:216258/150‑1 (MQ=255)
                                         tgctgGCTGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATAGTTATTCATAGGTTGTTCTCCCGGCTTAAGAGCAGGTTTGCTTGCCGGATGCGGCGAGAACGCCTTATCCGGCATACGGGGCGGAGCGAATGCAGGCGTCGATAtt                                                                                                             >  2:819570/4‑150 (MQ=255)
                                             ggCGGTTACAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTAGCCTGAAGCGGCGTGAACGACTTTTCCGGCCTACGGGGCGGTGCTAATGCAGGCGTCGTTATTTCGc                                                                                                         >  2:1622263/1‑150 (MQ=255)
                                                   tCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGa                                                                                                   <  1:558327/150‑1 (MQ=255)
                                                        gTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTtat                                                                                              <  1:1500569/150‑1 (MQ=255)
                                                         tCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTtata                                                                                             <  1:579251/150‑1 (MQ=255)
                                                           ggtatatAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTAtatt                                                                                           <  2:1167467/147‑1 (MQ=255)
                                                           ggCATATAGAGTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCGGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTTTCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTAtatt                                                                                           <  1:42222/150‑1 (MQ=255)
                                                              atatAGATTGAGATCAGCGGATTGATTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTAGAATCCGACTTAtattcgt                                                                                        >  1:1395416/1‑150 (MQ=255)
                                                                 taGATTGAGATCAGCGGATTGTTTTTCATTGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTAtattcgtatt                                                                                     <  1:799836/150‑1 (MQ=255)
                                                                  aGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTAtattcgtattc                                                                                    >  1:210346/1‑150 (MQ=255)
                                                                   gATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTAtattcgtattcg                                                                                   >  1:852853/1‑150 (MQ=255)
                                                                    aTTGAGATCAGCTGATTGTTTTTCATAGGTTGGCCTCCCGGCTTATGAGCGGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATattcgtattcgt                                                                                  <  2:719716/150‑1 (MQ=255)
                                                                    aTTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATattcgtattcgt                                                                                  <  2:1388966/150‑1 (MQ=255)
                                                                       gagaTCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAAt                                                                               <  2:369127/150‑1 (MQ=255)
                                                                          aTCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGc                                                                            <  1:680970/150‑1 (MQ=255)
                                                                                 gATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATc                                                                     >  2:733963/1‑150 (MQ=255)
                                                                                 gATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATc                                                                     >  2:770024/1‑150 (MQ=255)
                                                                                    tGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTg                                                                  <  2:631895/150‑1 (MQ=255)
                                                                                       ttttCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTATTAGCCATAAGCCTGATa                                                               >  2:1143658/1‑150 (MQ=255)
                                                                                       ttttCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATa                                                               <  1:580273/150‑1 (MQ=255)
                                                                                           cATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATAcgcg                                                           >  1:526257/1‑150 (MQ=255)
                                                                                             tAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGTGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCt                                                         <  2:590360/150‑1 (MQ=255)
                                                                                                gttgttCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGgc                                                      >  1:1144034/1‑150 (MQ=255)
                                                                                                 ttgttCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGgcg                                                     >  2:1698989/1‑150 (MQ=255)
                                                                                                     tctcCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCgcggc                                                 <  2:934948/150‑1 (MQ=255)
                                                                                                      gtcCGGGCTTATTAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATACGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTTTATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCgcggcg                                                <  2:1729324/149‑1 (MQ=255)
                                                                                                      ctcCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCgcggcg                                                >  2:129929/1‑150 (MQ=255)
                                                                                                           ggCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGa                                           >  1:1233646/1‑150 (MQ=255)
                                                                                                              ttATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATgg                                        <  1:1332580/150‑1 (MQ=255)
                                                                                                                aTGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAg                                      >  1:94192/1‑150 (MQ=255)
                                                                                                                    gCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCa                                  <  1:424014/150‑1 (MQ=255)
                                                                                                                      tGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGa                                >  1:1535576/1‑150 (MQ=255)
                                                                                                                            gcttgcCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCAcc                          >  2:77797/1‑150 (MQ=255)
                                                                                                                            gcttgcCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCAcc                          >  2:630076/1‑150 (MQ=255)
                                                                                                                             cttgcCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCAccc                         >  1:1093723/1‑150 (MQ=255)
                                                                                                                             cttgcCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCAccc                         <  2:1239582/150‑1 (MQ=255)
                                                                                                                              aggcCGGATAAGGCGTTCACGCCGCATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTAGTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCGGGCGCGGCGGAAGATGGAGTTCAGAATCAcccc                        >  1:508494/3‑150 (MQ=255)
                                                                                                                               tgcCGGATGCGGCGTGAACGCCTTCTCCGGCCGACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCAACCGACTTATATTCGTATGCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCt                       <  2:151383/150‑1 (MQ=255)
                                                                                                                               tgcCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCt                       >  2:1452500/1‑150 (MQ=255)
                                                                                                                                gcCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCtt                      >  1:1472168/1‑150 (MQ=255)
                                                                                                                                   ggATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCa                   >  1:620362/1‑150 (MQ=255)
                                                                                                                                   ggATGCGGCGTGAACGACTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCa                   >  1:1005902/1‑150 (MQ=255)
                                                                                                                                    gataaggcgTTCACGCCGCATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCAc                  >  2:172179/6‑150 (MQ=255)
                                                                                                                                        cggcgTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGTCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGa              >  1:1131287/1‑150 (MQ=255)
                                                                                                                                         ggcgTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGa                                           <  1:828789/120‑1 (MQ=255)
                                                                                                                                         ggcgTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGa                                           >  2:828789/1‑120 (MQ=255)
                                                                                                                                         ggcgTGAACGCCTAATACGGACTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCTCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGATGGCGCGGCGGAAGATGGAGTTCAGAAGCACCCCTTTCACCGCaa             >  1:1663793/1‑150 (MQ=255)
                                                                                                                                          gcgTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAAt            >  1:1531615/1‑150 (MQ=255)
                                                                                                                                          gcgTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAAt            >  1:400328/1‑150 (MQ=255)
                                                                                                                                              gAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAATACCg        <  1:1641128/150‑1 (MQ=255)
                                                                                                                                              gAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAATACCg        >  2:1100814/1‑150 (MQ=255)
                                                                                                                                                aCGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAATACCGtt      >  1:1246565/1‑150 (MQ=255)
                                                                                                                                                   ccTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGCTACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAATACCGTTTTg   <  1:1133948/150‑1 (MQ=255)
                                                                                                                                                   ccTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAATACCGTTTTg   >  1:1016178/1‑150 (MQ=255)
                                                                                                                                                    gttATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTTTTTCGCATCCGACTTATATTCGTATTCGTAATTGCCATAATCCTGATACGCGCTGGCTCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACTGGAAGACCGTTTTGc  <  1:1723658/149‑1 (MQ=255)
                                                                                                                                                    cTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAATACCGTTTTGc  <  2:489865/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AGTCCTGGCGCAAAACCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAATACCGTTTTGC  >  NC_000913/2133304‑2133601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: