Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,133,454 T→C 6.6% intergenic (‑57/+36) wcaA ← / ← wzc putative glycosyl transferase/colanic acid production tyrosine‑protein kinase; autokinase; Ugd phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,133,4540TC 6.6% 225.7 / 4.7 77intergenic (‑57/+36)wcaA/wzcputative glycosyl transferase/colanic acid production tyrosine‑protein kinase; autokinase; Ugd phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (38/33);  new base C (2/3);  total (41/36)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

GTCCTGGCGCAAAACCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAATACCGTTTTGCTC  >  NC_000913/2133305‑2133603
                                                                                                                                                     |                                                                                                                                                     
gTCCTGGCGCAAAACCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCtt                                                                                                                                                       >  2:1707349/1‑150 (MQ=255)
  ccTGGCGCAAAACCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                                                                     >  1:958362/1‑150 (MQ=255)
    tGGCGCAAAACCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATcc                                                                                                                                                   >  2:1332580/1‑150 (MQ=255)
    tGGCGCAAAACCGATTTTATTGCCCGAATCGCCAGGTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATcc                                                                                                                                                   >  1:1112849/1‑150 (MQ=255)
        gcAAAACCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                                               <  2:195305/150‑1 (MQ=255)
          aaaaCCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGGTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATAAGGCGTTCACGCCGCATCCGGcaag                                                                                                                                             <  1:1607724/150‑4 (MQ=255)
           aaaCCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATAAGGCGTTCACGCCGCATCCGGcaagc                                                                                                                                            <  1:24365/150‑5 (MQ=255)
             aCCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCATGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCACGGCTTATGAGCTGGTTTGCTTGCCGGTTGCGGCGTGAACGCCTTATCCGTCCTACgg                                                                                                                                          >  1:628401/1‑150 (MQ=255)
             aCCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGGTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATAAGGCGTGCACGCCGCATCCGGcaagcaa                                                                                                                                          <  1:839310/150‑7 (MQ=255)
               cGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACgggg                                                                                                                                        <  2:1684177/150‑1 (MQ=255)
                    ttATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTGCGGGGCGGTg                                                                                                                                   >  1:920840/1‑150 (MQ=255)
                    ttATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATACGTTGATCTCCAGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTCCGGGGCGGTg                                                                                                                                   >  2:1136960/1‑150 (MQ=255)
                         gCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGTATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAAt                                                                                                                              >  1:1167467/1‑150 (MQ=255)
                         gCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAAt                                                                                                                              >  1:729906/1‑150 (MQ=255)
                              aaTCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGCGATCAGCGGAGTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAgg                                                                                                                         <  1:944757/150‑1 (MQ=255)
                               aTCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGc                                                                                                                        <  1:1182184/150‑1 (MQ=255)
                                 cGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAACTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGcgt                                                                                                                      <  1:282849/150‑1 (MQ=255)
                                 cGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAACTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGcgt                                                                                                                      <  1:1587631/150‑1 (MQ=255)
                                  gCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGcgtc                                                                                                                     >  1:899191/1‑150 (MQ=255)
                                     aGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGtt                                                                                                                  <  2:715866/150‑1 (MQ=255)
                                       ttgttgGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATCGGGTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTAt                                                                                                                <  1:1597786/150‑1 (MQ=255)
                                       gtgttgGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTAt                                                                                                                <  1:1447466/149‑1 (MQ=255)
                                        tgttgGCGGTTCCTGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGTAGGCGTCGTTAtt                                                                                                               <  2:216258/150‑1 (MQ=255)
                                        tgctgGCTGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATAGTTATTCATAGGTTGTTCTCCCGGCTTAAGAGCAGGTTTGCTTGCCGGATGCGGCGAGAACGCCTTATCCGGCATACGGGGCGGAGCGAATGCAGGCGTCGATAtt                                                                                                               >  2:819570/4‑150 (MQ=255)
                                            ggCGGTTACAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTAGCCTGAAGCGGCGTGAACGACTTTTCCGGCCTACGGGGCGGTGCTAATGCAGGCGTCGTTATTTCGc                                                                                                           >  2:1622263/1‑150 (MQ=255)
                                                  tCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGa                                                                                                     <  1:558327/150‑1 (MQ=255)
                                                       gTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTtat                                                                                                <  1:1500569/150‑1 (MQ=255)
                                                        tCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTtata                                                                                               <  1:579251/150‑1 (MQ=255)
                                                          ggtatatAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTAtatt                                                                                             <  2:1167467/147‑1 (MQ=255)
                                                          ggCATATAGAGTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCGGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTTTCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTAtatt                                                                                             <  1:42222/150‑1 (MQ=255)
                                                             atatAGATTGAGATCAGCGGATTGATTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTAGAATCCGACTTAtattcgt                                                                                          >  1:1395416/1‑150 (MQ=255)
                                                                taGATTGAGATCAGCGGATTGTTTTTCATTGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTAtattcgtatt                                                                                       <  1:799836/150‑1 (MQ=255)
                                                                 aGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTAtattcgtattc                                                                                      >  1:210346/1‑150 (MQ=255)
                                                                  gATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTAtattcgtattcg                                                                                     >  1:852853/1‑150 (MQ=255)
                                                                   aTTGAGATCAGCTGATTGTTTTTCATAGGTTGGCCTCCCGGCTTATGAGCGGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATattcgtattcgt                                                                                    <  2:719716/150‑1 (MQ=255)
                                                                   aTTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATattcgtattcgt                                                                                    <  2:1388966/150‑1 (MQ=255)
                                                                      gagaTCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAAt                                                                                 <  2:369127/150‑1 (MQ=255)
                                                                         aTCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGc                                                                              <  1:680970/150‑1 (MQ=255)
                                                                                gATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATc                                                                       >  2:770024/1‑150 (MQ=255)
                                                                                gATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATc                                                                       >  2:733963/1‑150 (MQ=255)
                                                                                   tGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTg                                                                    <  2:631895/150‑1 (MQ=255)
                                                                                      ttttCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTATTAGCCATAAGCCTGATa                                                                 >  2:1143658/1‑150 (MQ=255)
                                                                                      ttttCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATa                                                                 <  1:580273/150‑1 (MQ=255)
                                                                                          cATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATAcgcg                                                             >  1:526257/1‑150 (MQ=255)
                                                                                            tAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGTGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCt                                                           <  2:590360/150‑1 (MQ=255)
                                                                                               gttgttCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGgc                                                        >  1:1144034/1‑150 (MQ=255)
                                                                                                ttgttCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGgcg                                                       >  2:1698989/1‑150 (MQ=255)
                                                                                                    tctcCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCgcggc                                                   <  2:934948/150‑1 (MQ=255)
                                                                                                     gtcCGGGCTTATTAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATACGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTTTATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCgcggcg                                                  <  2:1729324/149‑1 (MQ=255)
                                                                                                     ctcCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCgcggcg                                                  >  2:129929/1‑150 (MQ=255)
                                                                                                          ggCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGa                                             >  1:1233646/1‑150 (MQ=255)
                                                                                                             ttATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATgg                                          <  1:1332580/150‑1 (MQ=255)
                                                                                                               aTGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAg                                        >  1:94192/1‑150 (MQ=255)
                                                                                                                   gCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCa                                    <  1:424014/150‑1 (MQ=255)
                                                                                                                     tGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGa                                  >  1:1535576/1‑150 (MQ=255)
                                                                                                                           gcttgcCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCAcc                            >  2:77797/1‑150 (MQ=255)
                                                                                                                           gcttgcCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCAcc                            >  2:630076/1‑150 (MQ=255)
                                                                                                                            cttgcCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCAccc                           <  2:1239582/150‑1 (MQ=255)
                                                                                                                            cttgcCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCAccc                           >  1:1093723/1‑150 (MQ=255)
                                                                                                                             aggcCGGATAAGGCGTTCACGCCGCATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTAGTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCGGGCGCGGCGGAAGATGGAGTTCAGAATCAcccc                          >  1:508494/3‑150 (MQ=255)
                                                                                                                              tgcCGGATGCGGCGTGAACGCCTTCTCCGGCCGACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCAACCGACTTATATTCGTATGCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCt                         <  2:151383/150‑1 (MQ=255)
                                                                                                                              tgcCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCt                         >  2:1452500/1‑150 (MQ=255)
                                                                                                                               gcCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCtt                        >  1:1472168/1‑150 (MQ=255)
                                                                                                                                  ggATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCa                     >  1:620362/1‑150 (MQ=255)
                                                                                                                                  ggATGCGGCGTGAACGACTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCa                     >  1:1005902/1‑150 (MQ=255)
                                                                                                                                   gataaggcgTTCACGCCGCATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCAc                    >  2:172179/6‑150 (MQ=255)
                                                                                                                                       cggcgTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGTCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGa                >  1:1131287/1‑150 (MQ=255)
                                                                                                                                        ggcgTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGa                                             <  1:828789/120‑1 (MQ=255)
                                                                                                                                        ggcgTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGa                                             >  2:828789/1‑120 (MQ=255)
                                                                                                                                        ggcgTGAACGCCTAATACGGACTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCTCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGATGGCGCGGCGGAAGATGGAGTTCAGAAGCACCCCTTTCACCGCaa               >  1:1663793/1‑150 (MQ=255)
                                                                                                                                         gcgTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAAt              >  1:1531615/1‑150 (MQ=255)
                                                                                                                                         gcgTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAAt              >  1:400328/1‑150 (MQ=255)
                                                                                                                                             gAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAATACCg          <  1:1641128/150‑1 (MQ=255)
                                                                                                                                             gAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAATACCg          >  2:1100814/1‑150 (MQ=255)
                                                                                                                                               aCGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAATACCGtt        >  1:1246565/1‑150 (MQ=255)
                                                                                                                                                  ccTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGCTACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAATACCGTTTTg     <  1:1133948/150‑1 (MQ=255)
                                                                                                                                                  ccTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAATACCGTTTTg     >  1:1016178/1‑150 (MQ=255)
                                                                                                                                                   gttATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTTTTTCGCATCCGACTTATATTCGTATTCGTAATTGCCATAATCCTGATACGCGCTGGCTCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACTGGAAGACCGTTTTGc    <  1:1723658/149‑1 (MQ=255)
                                                                                                                                                   cTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAATACCGTTTTGc    <  2:489865/150‑1 (MQ=255)
                                                                                                                                                     tATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCTGAATACCGTTTTGCTc  >  2:1681794/1‑150 (MQ=255)
                                                                                                                                                     tATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAATACCGTTTTGCTc  <  1:217595/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTCCTGGCGCAAAACCGATTTTATTGCCCGAATCGCCAGTTGTTGGCGGTTCCAGGTCGGCATATAGATTGAGATCAGCGGATTGTTTTTCATAGGTTGTTCTCCCGGCTTATGAGCTGGTTTGCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGGCGGTGCGAATGCAGGCGTCGTTATTTCGCATCCGACTTATATTCGTATTCGTAATAGCCATAATCCTGATACGCGCTGGCGCGGCGGAAGATGGAGTTCAGAATCACCCCTTTCACCGGAATACCGTTTTGCTC  >  NC_000913/2133305‑2133603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: