Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,140,320 A→C 5.7% A431A (GCT→GCG asmA ← suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,140,3200AC 5.7% 291.7 / 5.2 98A431A (GCT→GCGasmAsuppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
Reads supporting (aligned to +/- strand):  ref base A (46/46);  new base C (2/4);  total (48/50)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

ACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACC  >  NC_000913/2140171‑2140469
                                                                                                                                                     |                                                                                                                                                     
acaTCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCAcca                                                                                                                                                       >  2:1584463/1‑150 (MQ=255)
  aTCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCATCCATGCGCGTGTCGGTCATTTCGACATGTGCCTTTCCTTGCCAGTTGTGGAGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCAccagc                                                                                                                                                     >  1:1664208/1‑150 (MQ=255)
      ccGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGt                                                                                                                                                 >  2:376387/1‑150 (MQ=255)
      ccGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTAATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACAGGAGAAATCACCATCCAGt                                                                                                                                                 >  1:1066228/1‑150 (MQ=255)
       cGCCATTACGCTCTACCGGTTGCTGAATCATCTGCTGGAAGTGCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTg                                                                                                                                                <  2:653176/150‑1 (MQ=255)
         ccATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCGAGTTGTGGCGGATTGCGTCGGCTTCTATGTCAGCACCGGAGAAATCACCAGCCAGTGAc                                                                                                                                              >  2:1278826/1‑150 (MQ=255)
         ccATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGAc                                                                                                                                              >  1:248971/1‑150 (MQ=255)
         ccATTACGCTATAACGCTTTCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTAGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATTCGTCGGCATCTATGTCAGCACCGGAGAAATAACCAGCCAGTGAc                                                                                                                                              <  1:149707/150‑1 (MQ=255)
          cATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCATGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACa                                                                                                                                             >  1:310128/1‑150 (MQ=255)
                 ctctACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTcc                                                                                                                                      <  2:507299/150‑1 (MQ=255)
                             cTGAATCATCTGCTGCAAGTTTAACCCTTCCATGCGCGTGTCGGTCATTTCGACATGGGCCTGTCCTTGCCAGTTTTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAAACACCAGCCAGTGACATTTTTCCGGTCAACGAAAt                                                                                                                          <  2:1091212/150‑1 (MQ=255)
                              tGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATc                                                                                                                         <  2:185973/150‑1 (MQ=255)
                                 atcatcTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCCGCCAGTGACATTTTTCCGGTCAACGAAATCGGa                                                                                                                      >  1:996428/1‑150 (MQ=255)
                                 atcatcTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCCGCCAGTGACATTTTTCCGGTCAACGAAATCGGa                                                                                                                      >  1:1480871/1‑150 (MQ=255)
                                 atcatcTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGa                                                                                                                      >  1:819376/1‑150 (MQ=255)
                                   catcTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATa                                                                                                                    >  2:1513688/1‑150 (MQ=255)
                                    atcTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCCGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAg                                                                                                                   <  2:1480871/150‑1 (MQ=255)
                                    atcTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCCGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAg                                                                                                                   <  2:996428/150‑1 (MQ=255)
                                       tgctgGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTa                                                                                                                <  1:805125/150‑1 (MQ=255)
                                       tgctgGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTa                                                                                                                <  1:1405698/150‑1 (MQ=255)
                                       tgctgGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTa                                                                                                                <  2:1099508/150‑1 (MQ=255)
                                       tgctgGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTa                                                                                                                <  2:575774/150‑1 (MQ=255)
                                        gctgGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTaa                                                                                                               >  1:426911/1‑150 (MQ=255)
                                          tgGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAAc                                                                                                             >  1:523921/1‑150 (MQ=255)
                                           ggAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACg                                                                                                            <  1:703338/150‑1 (MQ=255)
                                               gTTAATCCCTACCATCCGCGAGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGATGTGGCGGAATGCGTCGGCTACTATGTCAGCAACGGAGAAAAAACAATCCAGTGAAATTTTTCCCGTCAACCAAATCGCATAGTTTAACGCCtt                                                                                                        >  2:1095933/1‑150 (MQ=255)
                                                 tCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCa                                                                                                      >  2:1372449/1‑150 (MQ=255)
                                                    tCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGaa                                                                                                   <  1:1130134/150‑1 (MQ=255)
                                                     cccTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAAt                                                                                                  >  2:1633573/1‑150 (MQ=255)
                                                       cTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATgg                                                                                                >  1:423930/1‑150 (MQ=255)
                                                        ttCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGt                                                                                               <  2:1227955/150‑1 (MQ=255)
                                                          ccATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTAc                                                                                             <  2:1650750/150‑1 (MQ=255)
                                                          ccATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTAc                                                                                             >  1:466391/1‑150 (MQ=255)
                                                          ccATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTAc                                                                                             <  1:661908/150‑1 (MQ=255)
                                                          ccATGCGCGTGTCGGGCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTAc                                                                                             <  2:489927/150‑1 (MQ=255)
                                                           cATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGTCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTAcc                                                                                            <  1:727876/150‑1 (MQ=255)
                                                           cATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTAcc                                                                                            <  1:1178408/150‑1 (MQ=255)
                                                             tGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGTAATCGGATAGTTAAACGCCTTCAGAATGGTACCaa                                                                                          <  1:1345393/150‑1 (MQ=255)
                                                                gcgTGTCGGTCCTTTCGACATGGGACTGTCCGTGCCAGCTGTGGCAGAATGCGTCGGAATCTATGTCAGCACCGGAGAAATCACCCTCCCCGTCCATTTTAACGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAAtct                                                                                       <  2:355825/150‑1 (MQ=255)
                                                                gcgTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAAtct                                                                                       <  1:1158804/150‑1 (MQ=255)
                                                                gcgTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAAtct                                                                                       <  1:760406/150‑1 (MQ=255)
                                                                   tgtCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCCCCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTATCCGCCTTCAGAATGGTACCAATCTCaa                                                                                    >  2:1378791/1‑150 (MQ=255)
                                                                      cGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGt                                                                                 >  2:1610864/1‑150 (MQ=255)
                                                                         tCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGtttt                                                                              <  2:248971/150‑1 (MQ=255)
                                                                          cATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTc                                                                             >  2:963400/1‑150 (MQ=255)
                                                                                gACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATATATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAGCGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCg                                                                       >  1:919296/1‑150 (MQ=255)
                                                                                 aCATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTAAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGt                                                                      <  1:1141832/150‑1 (MQ=255)
                                                                                  cATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAATTCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTg                                                                     >  1:440772/1‑150 (MQ=255)
                                                                                  cATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGccacg                                                                     <  1:743857/150‑4 (MQ=255)
                                                                                   aTGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTgg                                                                    >  1:24835/1‑150 (MQ=255)
                                                                                    tgtgCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGc                                                                   >  1:1543506/1‑150 (MQ=255)
                                                                                       gCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTgg                                                                >  1:108251/1‑150 (MQ=255)
                                                                                       gCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTgg                                                                <  1:1361496/150‑1 (MQ=255)
                                                                                       gCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTgg                                                                <  1:1368128/150‑1 (MQ=255)
                                                                                          tGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAg                                                             <  1:794953/150‑1 (MQ=255)
                                                                                           gTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTCTTCCAGCCACGGCTGGaaaa                                                            <  2:1365682/150‑3 (MQ=255)
                                                                                           gTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCCGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTCTTCCAGCCACGGCTGGaaaa                                                            <  2:1397775/150‑3 (MQ=255)
                                                                                            tCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGtt                                                           <  2:1024003/150‑1 (MQ=255)
                                                                                            tCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaac                                                           <  2:1382302/150‑4 (MQ=255)
                                                                                             ccTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaacg                                                          <  1:1584463/150‑5 (MQ=255)
                                                                                             ccTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTCTCCAGCCGTGGCTGGAAGttt                                                          <  2:1006574/150‑1 (MQ=255)
                                                                                             ccTTCCCAGATGTGCCGGAAAGCGGCGGCAACTATGTCAGCACCGGAGAAATCACCAGCCACTGACCGTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGATTGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGttt                                                          <  2:177982/150‑1 (MQ=255)
                                                                                              cTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTa                                                         >  2:1699451/1‑150 (MQ=255)
                                                                                              cTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaacgt                                                         <  1:991397/150‑6 (MQ=255)
                                                                                              cTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaacgt                                                         <  1:1303732/150‑6 (MQ=255)
                                                                                              cTTGCCAGTTGGGGCGGAATGCGTCGGCATCTATGTCAGCACCGCAGAAATCACCAGCCAGTCACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaacgt                                                         <  1:473431/150‑6 (MQ=255)
                                                                                                tGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGaaaacgttg                                                       <  2:1086445/150‑8 (MQ=255)
                                                                                                tGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATc                                                       >  2:589746/1‑150 (MQ=255)
                                                                                                    aGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCCGCCGTGGCTGGAAGTTTATCcgcg                                                   >  2:1247989/1‑150 (MQ=255)
                                                                                                    aGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCcgcg                                                   >  1:316632/1‑150 (MQ=255)
                                                                                                       tgtgGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGATATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTGCAGACTGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGATGTTTATCCGCGGAt                                                >  1:252071/1‑150 (MQ=255)
                                                                                                       tgtgGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGAt                                                <  1:725049/150‑1 (MQ=255)
                                                                                                        gtgGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCATTCAGAATGCTACAAATCTCAACGTTTTCAAGCCTTGGCTGGAAGTTTATCCGCGGAtt                                               >  2:527690/1‑150 (MQ=255)
                                                                                                            cGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTAtt                                           <  2:1387136/150‑1 (MQ=255)
                                                                                                                          aTCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAg                             <  1:1454717/150‑1 (MQ=255)
                                                                                                                            cTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCg                           >  1:1186504/1‑150 (MQ=255)
                                                                                                                            cTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCg                           <  1:1513688/150‑1 (MQ=255)
                                                                                                                            cTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCg                           >  1:767595/1‑150 (MQ=255)
                                                                                                                             tATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGt                          >  1:1093440/1‑150 (MQ=255)
                                                                                                                               tGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGc                        >  1:1298069/1‑150 (MQ=255)
                                                                                                                                gTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGcc                       <  1:752694/150‑1 (MQ=255)
                                                                                                                                gTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGAGGAGTTATTGATGTCGCGTCCAGCGTGcc                       >  2:832553/1‑150 (MQ=255)
                                                                                                                                gTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCAGCGGATTTATTGATGTCGCGTCCAGCGTGcc                       >  1:1572132/1‑150 (MQ=255)
                                                                                                                                  cAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCACCGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCg                     >  2:972493/1‑150 (MQ=255)
                                                                                                                                  cAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCg                     >  1:580224/1‑150 (MQ=255)
                                                                                                                                    gCACCGGGGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGc                   >  1:441642/1‑150 (MQ=255)
                                                                                                                                       ccGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGATTGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTAGTGATGTCGCGTCCAGCGTGCACGGCatt                >  2:689216/1‑148 (MQ=255)
                                                                                                                                         ggAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGa              >  1:976205/1‑150 (MQ=255)
                                                                                                                                         ggAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGa              >  2:493123/1‑150 (MQ=255)
                                                                                                                                            gaAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAAc           <  1:989189/150‑1 (MQ=255)
                                                                                                                                             aaaTCACCCGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGATAcc          <  2:905655/150‑1 (MQ=255)
                                                                                                                                             aaaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAAcc          <  2:423930/150‑1 (MQ=255)
                                                                                                                                              aaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCt         <  1:1699451/150‑1 (MQ=255)
                                                                                                                                                tCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCg                           <  1:1719418/130‑1 (MQ=255)
                                                                                                                                                tCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCg                           >  2:1719418/1‑130 (MQ=255)
                                                                                                                                                 cACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTc      >  2:1268443/1‑150 (MQ=255)
                                                                                                                                                  aCCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTcc     >  2:986461/1‑150 (MQ=255)
                                                                                                                                                   ccagccagTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGACACCTGTCCa    >  2:1434916/1‑150 (MQ=255)
                                                                                                                                                   ccagccagTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCa    <  1:1633573/150‑1 (MQ=255)
                                                                                                                                                     agccagTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCAcc  >  1:1644604/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACATCACCGCCATTACGCTCTACCGCTTGCTGAATCATCTGCTGGAAGTTCATCCCTTCCATGCGCGTGTCGGTCATTTCGACATGTGCCTGTCCTTGCCAGTTGTGGCGGAATGCGTCGGCATCTATGTCAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACC  >  NC_000913/2140171‑2140469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: