Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,167,278 G→A 6.6% intergenic (+78/+24) cyaR → / ← ogrK sRNA antisense regulator of ompX mRNA instability, Hfq‑dependent, cAMP‑induced/orphan Ogr protein, positive regulator of P2 growth

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,167,2780GA 6.6% 329.6 / 8.3 106intergenic (+78/+24)cyaR/ogrKsRNA antisense regulator of ompX mRNA instability, Hfq‑dependent, cAMP‑induced/orphan Ogr protein, positive regulator of P2 growth
Reads supporting (aligned to +/- strand):  ref base G (59/40);  new base A (3/4);  total (62/44)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

Only 100 of 108 total aligned reads displayed.

CCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCACGTT  >  NC_000913/2167129‑2167427
                                                                                                                                                     |                                                                                                                                                     
ccATAAACTGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCg                                                                                                                                                       <  2:417820/150‑1 (MQ=255)
        tGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAggg                                                                                                                                               <  2:1442943/150‑1 (MQ=255)
        tGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAggg                                                                                                                                               <  2:1029819/150‑1 (MQ=255)
           tAGCTGTACCAGGAACCAACTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCAGTCGAGGGGCt                                                                                                                                            <  2:1106243/150‑1 (MQ=255)
             gCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTc                                                                                                                                          <  1:1182321/150‑1 (MQ=255)
             gCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTc                                                                                                                                          <  2:188441/150‑1 (MQ=255)
                gTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCATCGACTGAGGGGCTTCCTg                                                                                                                                       >  2:1431330/1‑150 (MQ=255)
                gTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCACTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTACACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTg                                                                                                                                       >  2:941190/1‑150 (MQ=255)
                 tACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCAGTCGAGGGGCttttttg                                                                                                                                      <  2:334940/150‑6 (MQ=255)
                  aCCAGGAACCACCTCCTTAGGCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGtt                                                                                                                                     <  1:1319093/150‑1 (MQ=255)
                  aCCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAACGCCCCTCGACTGAGGGGCTTCCTGtt                                                                                                                                     <  2:1132352/150‑1 (MQ=255)
                  aCCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGtt                                                                                                                                     >  1:1710242/1‑150 (MQ=255)
                  aCCAGGAACCACCACCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCAGTCGAGGGGCttttttgt                                                                                                                                     <  1:1047693/150‑7 (MQ=255)
                  aCCAGGAAACACCTCCTTAGCCTTTGTTATCTCCCTTACACGGGCTTATTTTTAACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGtt                                                                                                                                     <  2:1640217/150‑1 (MQ=255)
                   ccAGTAACCACCTACTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAATGATTTATTTCTGGTCACGTCCTCACATTGAGCACATCGAAATAAAAGCCCCTCGACTGAGGGGCTTCCTGttt                                                                                                                                    <  1:956046/150‑1 (MQ=255)
                   ccAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGttt                                                                                                                                    >  1:1386549/1‑150 (MQ=255)
                   ccAGGAACCACCTCCTTAGCCTGTGTAATCTCCCGTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACTTCCACACATTGCCCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGgtt                                                                                                                                    >  1:702807/1‑150 (MQ=255)
                    cAGGAACCACCTCCTTAGCTTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTc                                                                                                                                   <  1:1294306/150‑1 (MQ=255)
                    cAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGCCCACATCGACAAAAAAGCCCCTCGACTGCGGGGCTTCCTGTTTc                                                                                                                                   >  2:1220681/1‑150 (MQ=255)
                     aGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTCATACAATGAATTAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGTGCTTCCTGTTTCt                                                                                                                                  >  1:83320/1‑150 (MQ=255)
                     aGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCt                                                                                                                                  >  1:79136/1‑150 (MQ=255)
                         accaccTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAAtt                                                                                                                              <  2:626380/150‑1 (MQ=255)
                           caccTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTAc                                                                                                                            >  1:525720/1‑150 (MQ=255)
                           caccTCCTTAGCCTGTATAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTAc                                                                                                                            <  1:1433954/150‑1 (MQ=255)
                             cctcctTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACAt                                                                                                                          >  2:1163803/1‑150 (MQ=255)
                                cctTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCACTCGACTGAGGGGCTTCCTGTTTCTAATtacataca                                                                                                                       >  2:293755/1‑150 (MQ=255)
                                 ctTAGCCTGTGTAATCTCACTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCcac                                                                                                                      <  2:718095/150‑1 (MQ=255)
                                    aGCCTGTGTAATCTCCCTGACACGGGCTTATTTTTTACGCGAATTACAATGAAAGAAAAGGAGTTATTTCTGGTCACGTCAACACAGTGACCACATCGACAAAAAAGCCCCTAGACTGAGGGGCGTCCTGTTTCTAATTTCATCCACATa                                                                                                                   >  1:276522/1‑150 (MQ=255)
                                     gCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGGAATACAATGATATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTGCCTGTTTCTAATTACATCCACATaa                                                                                                                  >  1:1129451/1‑150 (MQ=255)
                                      ccTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAAt                                                                                                                 <  1:828546/150‑1 (MQ=255)
                                          tgtAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTtgc                                                                                                             >  1:643776/1‑150 (MQ=255)
                                          tgtAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTtgc                                                                                                             >  1:1056786/1‑150 (MQ=255)
                                           gtAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTtgct                                                                                                            >  2:1539838/1‑150 (MQ=255)
                                            tAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTtgctg                                                                                                           <  1:406291/150‑1 (MQ=255)
                                            tAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTtgctg                                                                                                           >  2:483805/1‑150 (MQ=255)
                                             aaTCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTtgctgc                                                                                                          <  1:696379/150‑1 (MQ=255)
                                                ctcCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCt                                                                                                       >  2:499338/1‑150 (MQ=255)
                                                ctcCCTAACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCt                                                                                                       >  2:1444532/1‑150 (MQ=255)
                                                  cccTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGa                                                                                                     >  2:831083/1‑150 (MQ=255)
                                                    cTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACg                                                                                                   <  1:1513071/150‑1 (MQ=255)
                                                    cTTACACGGGCTTATTTTTACGCGTAATACAATTAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACAACGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACgg                                                                                                  <  2:922714/150‑1 (MQ=255)
                                                     ttACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACgg                                                                                                  >  2:1406668/1‑150 (MQ=255)
                                                       acacGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACAACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTATAATTACATCAACATAATTTGCTGCCCAGACGTCa                                                                                                >  2:853653/1‑150 (MQ=255)
                                                          cgcgCTTATTTTTTACGAGAAAAACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACAATGACCACATCGACAAAAAAGCCCCTCGACTGAGTGGCTTCCTGTTTCTAATTACATCCAAATAATATGCTGCCATGACGGCAACg                                                                                             >  2:59331/4‑150 (MQ=255)
                                                           gggCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACgg                                                                                            >  2:330278/1‑150 (MQ=255)
                                                            ggCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACggg                                                                                           <  2:732720/150‑1 (MQ=255)
                                                            ggCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACggg                                                                                           <  2:1674822/150‑1 (MQ=255)
                                                              cTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACCTCGACAAAAAAGCCCCTCGACTGAGGGCCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTCACGGCAACGGGTg                                                                                         >  2:1281747/1‑150 (MQ=255)
                                                              cTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTg                                                                                         >  2:616649/1‑150 (MQ=255)
                                                              cTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCAACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGACATGACGGCAACGGGTg                                                                                         >  1:1317615/1‑150 (MQ=255)
                                                               ttATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGc                                                                                        <  1:235476/150‑1 (MQ=255)
                                                                     ttttCGCGTAATACAATGAAATAAAAGGATTTATTTCTGGGCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGATGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGGGCGGCCtt                                                                                  <  2:72381/150‑1 (MQ=255)
                                                                          gcgTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTacgac                                                                             >  1:559058/1‑150 (MQ=255)
                                                                          gcgTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTacgac                                                                             >  1:1367273/1‑150 (MQ=255)
                                                                                tACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGAc                                                                       >  2:356673/1‑150 (MQ=255)
                                                                                 aCAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACt                                                                      <  1:831083/150‑1 (MQ=255)
                                                                                  cAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACtt                                                                     >  1:129088/1‑150 (MQ=255)
                                                                                  cAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACtt                                                                     >  1:767541/1‑150 (MQ=255)
                                                                                  cAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACtt                                                                     <  1:1539838/150‑1 (MQ=255)
                                                                                    aTGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCATGACGGCAACGGGTGCGGCCTTACGCCGTGGtcttct                                                                   >  2:335996/1‑150 (MQ=255)
                                                                                       aaaTAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTccc                                                                <  1:330278/150‑1 (MQ=255)
                                                                                          tAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGc                                                             >  2:789265/1‑150 (MQ=255)
                                                                                            aaaGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCtt                                                           >  1:1595554/1‑150 (MQ=255)
                                                                                             aaGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTc                                                          >  2:1145033/1‑150 (MQ=255)
                                                                                             aaGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTc                                                          >  2:51113/1‑150 (MQ=255)
                                                                                              agtatttatttCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTTCGACGTGGACTTCTCCCGGCTTca                                                         <  1:843817/147‑1 (MQ=255)
                                                                                              aGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTca                                                         <  1:849493/150‑1 (MQ=255)
                                                                                               ggATTTATTTCTGGTCACGTCCACACATTGCCCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGCCGGCACCGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTcac                                                        >  1:1022903/1‑150 (MQ=255)
                                                                                                   ttatttCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATg                                                    <  1:223752/150‑1 (MQ=255)
                                                                                                       ttCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATc                                                >  2:1114482/1‑150 (MQ=255)
                                                                                                        tCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCg                                               >  1:738375/1‑150 (MQ=255)
                                                                                                         cTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGc                                              >  2:1194029/1‑150 (MQ=255)
                                                                                                               aCGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTACCTGGTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTAcc                                        <  1:868415/150‑1 (MQ=255)
                                                                                                                cGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGTGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGCCGGCTACGGGTGCGGCCTTACGACGTGGCCTGCTCCCGGCTTCACAATGTATCGCTGTACCg                                       >  2:958482/1‑150 (MQ=255)
                                                                                                                       acaTTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATa                                <  1:1571944/150‑1 (MQ=255)
                                                                                                                       acaTTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATa                                <  1:279756/150‑1 (MQ=255)
                                                                                                                        caTTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATaa                               >  2:455161/1‑150 (MQ=255)
                                                                                                                            gACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACAAAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTATACCGACTCATAAGtga                           >  1:333712/1‑150 (MQ=255)
                                                                                                                               cacaTCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGtgatga                        >  1:1610240/1‑150 (MQ=255)
                                                                                                                                acaTCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGaa                       <  2:779655/150‑1 (MQ=255)
                                                                                                                                   tCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACgg                    >  1:644979/1‑149 (MQ=255)
                                                                                                                                   tCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGt                    >  1:719765/1‑150 (MQ=255)
                                                                                                                                       cAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGgcg                <  1:483805/150‑1 (MQ=255)
                                                                                                                                         aaaaaGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCt              >  2:1189975/1‑150 (MQ=255)
                                                                                                                                         aaaaaGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCt              >  2:1129328/1‑150 (MQ=255)
                                                                                                                                          ggaaGCCCCTCAGTCGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAAGGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTg             >  1:907701/3‑150 (MQ=255)
                                                                                                                                          ggaaGCCCCTCAGTCGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGtgatg                         >  1:945912/3‑138 (MQ=255)
                                                                                                                                          ggaaGCCCCTCAGTCGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGtgatg                         <  2:945912/136‑1 (MQ=255)
                                                                                                                                          aataGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCGACATAATTTGCTGCCCTGACGGCAACGGGTTCGGCCTTACGACGTGGACTTCTCCCGGCTTCTCAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTg             <  2:1205027/147‑1 (MQ=255)
                                                                                                                                           gaaGCCCCTCAGTCGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCCCTGTACCGACTCATAAGTGATGAACGTGGCGCTGc            >  1:1193211/2‑150 (MQ=255)
                                                                                                                                              gCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAAt         >  2:1730059/1‑150 (MQ=255)
                                                                                                                                               ccccTCGACTGAGGGGCTTCCTGTTTCTTATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAAtt        >  2:140991/1‑150 (MQ=255)
                                                                                                                                               ccccTCGACTGAGGGGCTACCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTAGCGACTCATAAGTGATGAACGTGGCGCTGCAAtt        >  1:725063/1‑150 (MQ=255)
                                                                                                                                                cccTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTc       <  2:1386549/150‑1 (MQ=255)
                                                                                                                                                 ccTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTcc      >  2:1430429/1‑149 (MQ=255)
                                                                                                                                                 ccTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCa      >  2:823065/1‑150 (MQ=255)
                                                                                                                                                 ccTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCa      <  1:1616850/150‑1 (MQ=255)
                                                                                                                                                  cTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCAc     <  1:356673/150‑1 (MQ=255)
                                                                                                                                                  cTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCAc     <  1:853653/150‑1 (MQ=255)
                                                                                                                                                     gACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCAAAAGTGATGAACGTGGCGCTTCAAATCACGtt  >  1:274431/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCATAAAATGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCACGTT  >  NC_000913/2167129‑2167427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: