Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,167,281 T→C 6.4% intergenic (+81/+21) cyaR → / ← ogrK sRNA antisense regulator of ompX mRNA instability, Hfq‑dependent, cAMP‑induced/orphan Ogr protein, positive regulator of P2 growth

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,167,2810TC 6.4% 324.7 / 9.6 109intergenic (+81/+21)cyaR/ogrKsRNA antisense regulator of ompX mRNA instability, Hfq‑dependent, cAMP‑induced/orphan Ogr protein, positive regulator of P2 growth
Reads supporting (aligned to +/- strand):  ref base T (60/42);  new base C (3/4);  total (63/46)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01

Only 100 of 111 total aligned reads displayed.

TGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCACGTTCTG  >  NC_000913/2167137‑2167430
                                                                                                                                                |                                                                                                                                                     
tGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAggg                                                                                                                                                  <  2:1442943/150‑1 (MQ=255)
tGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAggg                                                                                                                                                  <  2:1029819/150‑1 (MQ=255)
   tAGCTGTACCAGGAACCAACTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCAGTCGAGGGGCt                                                                                                                                               <  2:1106243/150‑1 (MQ=255)
     gCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTc                                                                                                                                             <  1:1182321/150‑1 (MQ=255)
     gCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTc                                                                                                                                             <  2:188441/150‑1 (MQ=255)
        gTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCATCGACTGAGGGGCTTCCTg                                                                                                                                          >  2:1431330/1‑150 (MQ=255)
        gTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCACTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTACACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTg                                                                                                                                          >  2:941190/1‑150 (MQ=255)
         tACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCAGTCGAGGGGCttttttg                                                                                                                                         <  2:334940/150‑6 (MQ=255)
          aCCAGGAACCACCTCCTTAGGCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGtt                                                                                                                                        <  1:1319093/150‑1 (MQ=255)
          aCCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGtt                                                                                                                                        >  1:1710242/1‑150 (MQ=255)
          aCCAGGAACCACCACCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCAGTCGAGGGGCttttttgt                                                                                                                                        <  1:1047693/150‑7 (MQ=255)
          aCCAGGAAACACCTCCTTAGCCTTTGTTATCTCCCTTACACGGGCTTATTTTTAACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGtt                                                                                                                                        <  2:1640217/150‑1 (MQ=255)
           ccAGTAACCACCTACTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAATGATTTATTTCTGGTCACGTCCTCACATTGAGCACATCGAAATAAAAGCCCCTCGACTGAGGGGCTTCCTGttt                                                                                                                                       <  1:956046/150‑1 (MQ=255)
           ccAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGttt                                                                                                                                       >  1:1386549/1‑150 (MQ=255)
           ccAGGAACCACCTCCTTAGCCTGTGTAATCTCCCGTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACTTCCACACATTGCCCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGgtt                                                                                                                                       >  1:702807/1‑150 (MQ=255)
            cAGGAACCACCTCCTTAGCTTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTc                                                                                                                                      <  1:1294306/150‑1 (MQ=255)
            cAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGCCCACATCGACAAAAAAGCCCCTCGACTGCGGGGCTTCCTGTTTc                                                                                                                                      >  2:1220681/1‑150 (MQ=255)
             aGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTCATACAATGAATTAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGTGCTTCCTGTTTCt                                                                                                                                     >  1:83320/1‑150 (MQ=255)
             aGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCt                                                                                                                                     >  1:79136/1‑150 (MQ=255)
                 accaccTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAAtt                                                                                                                                 <  2:626380/150‑1 (MQ=255)
                   caccTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTAc                                                                                                                               >  1:525720/1‑150 (MQ=255)
                   caccTCCTTAGCCTGTATAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTAc                                                                                                                               <  1:1433954/150‑1 (MQ=255)
                     cctcctTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACAt                                                                                                                             >  1:732720/1‑150 (MQ=255)
                     cctcctTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACAt                                                                                                                             >  2:1163803/1‑150 (MQ=255)
                        cctTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCACTCGACTGAGGGGCTTCCTGTTTCTAATtacataca                                                                                                                          >  2:293755/1‑150 (MQ=255)
                         ctTAGCCTGTGTAATCTCACTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCcac                                                                                                                         <  2:718095/150‑1 (MQ=255)
                            aGCCTGTGTAATCTCCCTGACACGGGCTTATTTTTTACGCGAATTACAATGAAAGAAAAGGAGTTATTTCTGGTCACGTCAACACAGTGACCACATCGACAAAAAAGCCCCTAGACTGAGGGGCGTCCTGTTTCTAATTTCATCCACATa                                                                                                                      >  1:276522/1‑150 (MQ=255)
                             gCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGGAATACAATGATATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTGCCTGTTTCTAATTACATCCACATaa                                                                                                                     >  1:1129451/1‑150 (MQ=255)
                              ccTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAAt                                                                                                                    <  1:828546/150‑1 (MQ=255)
                                  tgtAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTtgc                                                                                                                >  1:643776/1‑150 (MQ=255)
                                  tgtAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTtgc                                                                                                                >  1:1056786/1‑150 (MQ=255)
                                   gtAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTtgct                                                                                                               >  2:1539838/1‑150 (MQ=255)
                                    tAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTtgctg                                                                                                              <  1:406291/150‑1 (MQ=255)
                                     aaTCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTtgctgc                                                                                                             <  1:696379/150‑1 (MQ=255)
                                        ctcCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCt                                                                                                          >  2:499338/1‑150 (MQ=255)
                                        ctcCCTAACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCt                                                                                                          >  2:1444532/1‑150 (MQ=255)
                                          cccTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGa                                                                                                        >  2:831083/1‑150 (MQ=255)
                                            cTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACg                                                                                                      <  1:1513071/150‑1 (MQ=255)
                                            cTTACACGGGCTTATTTTTACGCGTAATACAATTAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACAACGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACgg                                                                                                     <  2:922714/150‑1 (MQ=255)
                                               acacGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACAACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTATAATTACATCAACATAATTTGCTGCCCAGACGTCa                                                                                                   >  2:853653/1‑150 (MQ=255)
                                                  cgcgCTTATTTTTTACGAGAAAAACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACAATGACCACATCGACAAAAAAGCCCCTCGACTGAGTGGCTTCCTGTTTCTAATTACATCCAAATAATATGCTGCCATGACGGCAACg                                                                                                >  2:59331/4‑150 (MQ=255)
                                                   gggCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACgg                                                                                               >  2:330278/1‑150 (MQ=255)
                                                    ggCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACggg                                                                                              <  2:1674822/150‑1 (MQ=255)
                                                    ggCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACggg                                                                                              <  2:732720/150‑1 (MQ=255)
                                                      cTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACCTCGACAAAAAAGCCCCTCGACTGAGGGCCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTCACGGCAACGGGTg                                                                                            >  2:1281747/1‑150 (MQ=255)
                                                      cTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTg                                                                                            >  2:616649/1‑150 (MQ=255)
                                                      cTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCAACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGACATGACGGCAACGGGTg                                                                                            >  1:1317615/1‑150 (MQ=255)
                                                       ttATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGc                                                                                           <  1:235476/150‑1 (MQ=255)
                                                             ttttCGCGTAATACAATGAAATAAAAGGATTTATTTCTGGGCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGATGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGGGCGGCCtt                                                                                     <  2:72381/150‑1 (MQ=255)
                                                                  gcgTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTacgac                                                                                >  1:559058/1‑150 (MQ=255)
                                                                        tACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGAc                                                                          >  2:356673/1‑150 (MQ=255)
                                                                         aCAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACt                                                                         <  1:831083/150‑1 (MQ=255)
                                                                          cAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACtt                                                                        >  1:767541/1‑150 (MQ=255)
                                                                          cAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACtt                                                                        >  1:129088/1‑150 (MQ=255)
                                                                          cAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACtt                                                                        >  1:1500552/1‑150 (MQ=255)
                                                                          cAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACtt                                                                        <  1:1539838/150‑1 (MQ=255)
                                                                            aTGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCATGACGGCAACGGGTGCGGCCTTACGCCGTGGtcttct                                                                      >  2:335996/1‑150 (MQ=255)
                                                                               aaaTAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTccc                                                                   <  1:330278/150‑1 (MQ=255)
                                                                                  tAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGc                                                                >  2:789265/1‑150 (MQ=255)
                                                                                    aaaGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCtt                                                              >  1:1595554/1‑150 (MQ=255)
                                                                                    aaaGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCtt                                                              <  1:949191/150‑1 (MQ=255)
                                                                                     aaGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTc                                                             >  2:1145033/1‑150 (MQ=255)
                                                                                     aaGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTc                                                             >  2:51113/1‑150 (MQ=255)
                                                                                      agtatttatttCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTTCGACGTGGACTTCTCCCGGCTTca                                                            <  1:843817/147‑1 (MQ=255)
                                                                                      aGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTca                                                            <  1:849493/150‑1 (MQ=255)
                                                                                       ggATTTATTTCTGGTCACGTCCACACATTGCCCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGCCGGCACCGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTcac                                                           >  1:1022903/1‑150 (MQ=255)
                                                                                           ttatttCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATg                                                       <  1:223752/150‑1 (MQ=255)
                                                                                               ttCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATc                                                   >  2:1114482/1‑150 (MQ=255)
                                                                                                tCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCg                                                  >  1:738375/1‑150 (MQ=255)
                                                                                                 cTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGc                                                 >  2:1194029/1‑150 (MQ=255)
                                                                                                       aCGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTACCTGGTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTAcc                                           <  1:868415/150‑1 (MQ=255)
                                                                                                        cGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGTGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGCCGGCTACGGGTGCGGCCTTACGACGTGGCCTGCTCCCGGCTTCACAATGTATCGCTGTACCg                                          >  2:958482/1‑150 (MQ=255)
                                                                                                        cGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTGCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTGCCGGCTTCACAATGTATCGCTGTACCg                                          >  1:281558/1‑150 (MQ=255)
                                                                                                               acaTTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATa                                   >  2:287372/1‑150 (MQ=255)
                                                                                                               acaTTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATa                                   <  1:279756/150‑1 (MQ=255)
                                                                                                               acaTTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATa                                   <  1:1571944/150‑1 (MQ=255)
                                                                                                                caTTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATaa                                  >  2:455161/1‑150 (MQ=255)
                                                                                                                  ttGACCACAACGCCACAAAAGCCCCTCGCCTGAGGGGCTTCCTGGTTCTAATTACATCCACATACTTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCCTAAGt                                <  2:702807/150‑1 (MQ=255)
                                                                                                                    gACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACAAAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTATACCGACTCATAAGtga                              >  1:333712/1‑150 (MQ=255)
                                                                                                                       cacaTCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGtgatga                           >  1:1610240/1‑150 (MQ=255)
                                                                                                                        acaTCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGaa                          <  2:779655/150‑1 (MQ=255)
                                                                                                                           tCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACgg                       >  1:644979/1‑149 (MQ=255)
                                                                                                                           tCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGt                       >  1:719765/1‑150 (MQ=255)
                                                                                                                               cAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGgcg                   <  1:483805/150‑1 (MQ=255)
                                                                                                                                 aaaaaGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCt                 >  2:1189975/1‑150 (MQ=255)
                                                                                                                                 aaaaaGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCt                 >  2:1129328/1‑150 (MQ=255)
                                                                                                                                  ggaaGCCCCTCAGTCGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAAGGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTg                >  1:907701/3‑150 (MQ=255)
                                                                                                                                  ggaaGCCCCTCAGTCGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGtgatg                            >  1:945912/3‑138 (MQ=255)
                                                                                                                                  ggaaGCCCCTCAGTCGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGtgatg                            <  2:945912/136‑1 (MQ=255)
                                                                                                                                  aataGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCGACATAATTTGCTGCCCTGACGGCAACGGGTTCGGCCTTACGACGTGGACTTCTCCCGGCTTCTCAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTg                <  2:1205027/147‑1 (MQ=255)
                                                                                                                                   gaaGCCCCTCAGTCGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCCCTGTACCGACTCATAAGTGATGAACGTGGCGCTGc               >  1:1193211/2‑150 (MQ=255)
                                                                                                                                       ccccTCGACTGAGGGGCTTCCTGTTTCTTATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAAtt           >  2:140991/1‑150 (MQ=255)
                                                                                                                                        cccTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTc          <  2:1386549/150‑1 (MQ=255)
                                                                                                                                         ccTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTcc         >  2:1430429/1‑149 (MQ=255)
                                                                                                                                         ccTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCa         >  2:823065/1‑150 (MQ=255)
                                                                                                                                          cTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACATGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCAc        <  2:1317615/150‑1 (MQ=255)
                                                                                                                                             gACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCAAAAGTGATGAACGTGGCGCTTCAAATCACGtt     >  1:274431/1‑150 (MQ=255)
                                                                                                                                              aCTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCACGTTc    <  2:1535331/150‑1 (MQ=255)
                                                                                                                                               cTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCACGTTCt   <  1:1406668/150‑1 (MQ=255)
                                                                                                                                                tGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCACGTTCTg  <  2:354294/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
TGCTAGCTGTACCAGGAACCACCTCCTTAGCCTGTGTAATCTCCCTTACACGGGCTTATTTTTTACGCGTAATACAATGAAATAAAAGGATTTATTTCTGGTCACGTCCACACATTGACCACATCGACAAAAAAGCCCCTCGACTGAGGGGCTTCCTGTTTCTAATTACATCCACATAATTTGCTGCCCTGACGGCAACGGGTGCGGCCTTACGACGTGGACTTCTCCCGGCTTCACAATGTATCGCTGTACCGACTCATAAGTGATGAACGTGGCGCTGCAATTCACGTTCTG  >  NC_000913/2167137‑2167430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: