Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,168,420 A→T 6.2% intergenic (‑114/‑292) yegR ← / → yegS uncharacterized protein/phosphatidylglycerol kinase, metal‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,168,4200AT 6.2% 371.9 / 10.6 129intergenic (‑114/‑292)yegR/yegSuncharacterized protein/phosphatidylglycerol kinase, metal‑dependent
Reads supporting (aligned to +/- strand):  ref base A (58/63);  new base T (5/3);  total (63/66)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-01

Only 100 of 132 total aligned reads displayed.

ACTAATTAATGATATAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCC  >  NC_000913/2168274‑2168569
                                                                                                                                                  |                                                                                                                                                     
aCTAATTAATGATATAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTATTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATaa                                                                                                                                                    <  1:74117/150‑1 (MQ=255)
 cTAATTAATGATATAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAAATGAATAGGATTATAATAAATGTTCTGTAAAACATTTCataaataat                                                                                                                                                   >  1:1648142/1‑149 (MQ=255)
  taattaatGATATAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGt                                                                                                                                                  >  1:35598/1‑150 (MQ=255)
  taattaatGATATAGCAGCTATCTTATTCAACTCACCTTCCCCCATAAAATACCAACCACACTCACTAGTGTTTAAATATAATAATGGCATGATTATTATATTTGAATAGGATTATAATAAATGATCTTTTCAACAATTCCTAAAAAAga                                                                                                                                                  >  2:1054215/1‑149 (MQ=255)
   aattaatGATATAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTa                                                                                                                                                 >  2:461339/1‑150 (MQ=255)
   aattaatGATATAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTa                                                                                                                                                 <  1:1506367/150‑1 (MQ=255)
    attaatGATATAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAg                                                                                                                                                <  1:752828/150‑1 (MQ=255)
    attaatGATATAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAg                                                                                                                                                >  1:1216735/1‑150 (MQ=255)
       aatGATATAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGaa                                                                                                                                             >  1:906484/1‑150 (MQ=255)
        atGATATAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAAt                                                                                                                                            <  1:1616445/150‑1 (MQ=255)
         tGATATAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGAACAACATTTCCTACTTATGTAGGaaat                                                                                                                                           <  2:1102527/150‑3 (MQ=255)
          tatatAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTa                                                                                                                                          <  1:832986/149‑1 (MQ=255)
           atatAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACTTATGTAGGaaatgt                                                                                                                                         <  1:697877/150‑5 (MQ=255)
            tataGCAGCTATATTTTTCATCTCACCTTCACCCATTAAATACCAACGACACACTCTAGTGTTAAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACg                                                                                                                                        >  1:1049199/1‑150 (MQ=255)
              ttgcagcTTTATTTTTCATCTCACCTTCCGCTATTAAATACCATCGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTTTAATAAATGTTCTGTACAACTTTTCCTACATAAGTAGGAATTACGGa                                                                                                                                      <  2:881478/148‑1 (MQ=255)
                 cagcTATCTTTTTCATCTCACCTTCCCACATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACAt                                                                                                                                   <  2:791135/150‑1 (MQ=255)
                   tcTATCTTTTGCATCTCACCTTCCGCCATTAAATACCAACGAAACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGGTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTg                                                                                                                                 <  1:1518098/149‑1 (MQ=255)
                   gcTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTg                                                                                                                                 <  2:1141094/150‑1 (MQ=255)
                    cTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGa                                                                                                                                >  1:639851/1‑150 (MQ=255)
                      aTCTTTTTCTTCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAgg                                                                                                                              <  2:862212/150‑1 (MQ=255)
                      aTCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGATTAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAgg                                                                                                                              >  1:828460/1‑150 (MQ=255)
                          ttttCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGGCATTGAGGCCCt                                                                                                                          >  1:33732/1‑150 (MQ=255)
                           tttCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATAACGGACATTGAGGCCCtt                                                                                                                         >  2:1228534/1‑150 (MQ=255)
                             tCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCa                                                                                                                       <  1:538377/150‑1 (MQ=255)
                              aaTCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAg                                                                                                                      <  2:1667598/149‑1 (MQ=255)
                                tctcACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCGTGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAggg                                                                                                                    <  1:133500/150‑1 (MQ=255)
                                tctcACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAggg                                                                                                                    >  1:575143/1‑150 (MQ=255)
                                 ctcACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGt                                                                                                                   <  2:914942/150‑1 (MQ=255)
                                 atcACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGt                                                                                                                   <  2:598288/149‑1 (MQ=255)
                                  tcACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTa                                                                                                                  <  2:752898/150‑1 (MQ=255)
                                  tcACCTTACCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTa                                                                                                                  <  1:1551271/150‑1 (MQ=255)
                                  tcAACTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTa                                                                                                                  <  2:576863/150‑1 (MQ=255)
                                   cACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTaa                                                                                                                 >  2:1130274/1‑150 (MQ=255)
                                    aCCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAAc                                                                                                                <  2:685532/150‑1 (MQ=255)
                                     ccTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACt                                                                                                               >  1:1392849/1‑150 (MQ=255)
                                          ccccATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATg                                                                                                          >  2:410071/1‑150 (MQ=255)
                                           cccATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATgg                                                                                                         <  1:746455/150‑1 (MQ=255)
                                            ccATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATggg                                                                                                        <  1:589905/150‑1 (MQ=255)
                                            ccATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATggg                                                                                                        >  1:1695858/1‑150 (MQ=255)
                                                  aaTACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCttt                                                                                                  <  2:712630/150‑1 (MQ=255)
                                                  aaTACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTACTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCttt                                                                                                  <  2:788854/150‑1 (MQ=255)
                                                  aaTACCAACGACACTAGCTAGTGTATAAATATAATAATGGCATGATTATTATAATTGAAAAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCttt                                                                                                  <  2:302661/150‑1 (MQ=255)
                                                   aTACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTa                                                                                                 >  2:1571075/1‑150 (MQ=255)
                                                      ccAACGACACTCTCTAGTGTTTAAATATAATAATGTCAAGATTATTATATATGAAAAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCACTTCAGGGTAAATCAATGGTGGCTTTAata                                                                                              >  1:1040086/1‑150 (MQ=255)
                                                       cAAAGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAatat                                                                                             <  2:1457060/150‑1 (MQ=255)
                                                          cGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCATACATAAGTAGGAATTACGGAATTTGAGGCCATTAAGGTTAAATCCATGGGGGCTTTAAtatatt                                                                                          >  1:690504/1‑150 (MQ=255)
                                                           gACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTCAAtataata                                                                                         <  1:1130274/150‑1 (MQ=255)
                                                            acacTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAAtatattat                                                                                        <  1:1023780/150‑1 (MQ=255)
                                                             cacTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAAtatattata                                                                                       <  1:461339/150‑1 (MQ=255)
                                                                  tctAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTATGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGTCTTTAATATATTATATTGaa                                                                                  >  1:894100/1‑150 (MQ=255)
                                                                    tAGTGTTTAAATATAATTATGGAATGATTATTATTATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACAATGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGa                                                                                <  2:1531151/150‑1 (MQ=255)
                                                                    tAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGa                                                                                <  2:1013148/150‑1 (MQ=255)
                                                                      gtgtTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATg                                                                              <  2:1656528/150‑1 (MQ=255)
                                                                       tgtTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGc                                                                             >  2:1287125/1‑150 (MQ=255)
                                                                          ttAAATATAATAATGGCATGATTATTATATTTGAATAGGATTATAATAAATGTTCTTTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCACTTCAGGGTAACTACATGGGGGCTTTAATATATTATATTCAAGATGCCAc                                                                          >  1:119490/1‑150 (MQ=255)
                                                                           tAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACt                                                                         >  1:700625/1‑150 (MQ=255)
                                                                               tataATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGttt                                                                     <  2:546083/150‑1 (MQ=255)
                                                                                 taataaTGTCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAg                                                                   <  1:814477/150‑1 (MQ=255)
                                                                                 taataaTGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAg                                                                   <  1:63774/150‑1 (MQ=255)
                                                                                 taataaTGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAg                                                                   <  1:1518759/150‑1 (MQ=255)
                                                                                 taataaTGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAg                                                                   >  2:1417368/1‑150 (MQ=255)
                                                                                    taatGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTg                                                                <  2:906484/150‑1 (MQ=255)
                                                                                            tGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAg                                                        >  2:37634/1‑150 (MQ=255)
                                                                                             gATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAgg                                                       <  1:1413563/150‑1 (MQ=255)
                                                                                               ttattaTAATTGAATAGGATTATAATTAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGta                                                     <  2:62884/150‑1 (MQ=255)
                                                                                                    ataATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTAAGGACATTGAGGCCCTTCAGGGTAAATCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATga                                                >  1:870048/1‑149 (MQ=255)
                                                                                                     taATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGct                                               >  1:473115/1‑150 (MQ=255)
                                                                                                          gAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTTAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCtttt                                          >  2:641868/1‑150 (MQ=255)
                                                                                                            aTAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCtttttt                                        <  2:950431/150‑1 (MQ=255)
                                                                                                             tAGGATTATAATTAATGTTCTGTACAACATTTCCTAAATAAGTATGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTg                                       <  2:135683/150‑1 (MQ=255)
                                                                                                              aGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGATTATTAGGTATATGCTCTTTTTTGa                                      >  2:476986/1‑150 (MQ=255)
                                                                                                               ggATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGTCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGaa                                     <  1:1287125/150‑1 (MQ=255)
                                                                                                               ggATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGaa                                     >  2:1162039/1‑150 (MQ=255)
                                                                                                                  ttATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAAtt                                  <  2:1392849/150‑1 (MQ=255)
                                                                                                                     taataaATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTAt                               >  1:1402845/1‑150 (MQ=255)
                                                                                                                      aataaATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATc                              >  1:227883/1‑150 (MQ=255)
                                                                                                                      aataaATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATc                              >  1:791163/1‑150 (MQ=255)
                                                                                                                      aataaATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATc                              >  1:804922/1‑150 (MQ=255)
                                                                                                                       ataaATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCg                             <  2:781532/150‑1 (MQ=255)
                                                                                                                       ataaATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCg                             <  2:35598/150‑1 (MQ=255)
                                                                                                                       ataaATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCg                             <  2:924493/150‑1 (MQ=255)
                                                                                                                        taaATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCgg                            >  1:900100/1‑150 (MQ=255)
                                                                                                                          aaTGTTCTGTACAACATTTCCTACAAAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGCGGCTTTAAAATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTg                          <  2:1071847/150‑1 (MQ=255)
                                                                                                                             gTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCa                       >  1:1715675/1‑150 (MQ=255)
                                                                                                                              ttCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAg                      >  1:1618276/1‑150 (MQ=255)
                                                                                                                                cTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATTCTCTTTTTTGAAATTTATAGTTGGCAGcc                    >  1:622194/1‑150 (MQ=255)
                                                                                                                                  gTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGt                  >  2:619/1‑150 (MQ=255)
                                                                                                                                    acaacaTTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTa                >  2:395051/1‑150 (MQ=255)
                                                                                                                                     ccgtaattCCTACTTATGTAGGAATTACGGACAATGAGGCCCTTCAGGGTAACTCCATGGGGGCTGGAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAAATTATCGGTGGCAGCCGTTAg               >  1:333625/7‑150 (MQ=255)
                                                                                                                                     caacaTTTCCTACATAAGTATTAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATAGCGGCTTTAATATATTATATTGAAGATGCCACTGTTTTGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAg               <  1:128230/150‑1 (MQ=255)
                                                                                                                                     caacaTTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAg               >  2:332248/1‑150 (MQ=255)
                                                                                                                                        taattCCTACTTATGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTAt            >  2:1479558/4‑150 (MQ=255)
                                                                                                                                        taattCCTACTTATGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTAt            >  2:496385/4‑150 (MQ=255)
                                                                                                                                          attCCTACTTATGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTc          >  1:990781/2‑150 (MQ=255)
                                                                                                                                            tCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGc        <  2:1695858/150‑1 (MQ=255)
                                                                                                                                             ccTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCt       >  2:155547/1‑150 (MQ=255)
                                                                                                                                              atACATAAGTACTAATTCCTGAGATTGAGTCCCTTCATTGTAAATCAATTGCGTCGATACTATCTTTAATTGAAGATGCCTCTTTTTACTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTg      <  2:270062/149‑1 (MQ=255)
                                                                                                                                                aCATAAGTAGGAATAACGGACATTGAGGCCCTTCAGGGTAACTCCATTGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTc    <  1:1228534/150‑1 (MQ=255)
                                                                                                                                                  aTAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTccc  <  1:442591/150‑1 (MQ=255)
                                                                                                                                                  aTAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTccc  <  2:1250086/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
ACTAATTAATGATATAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATGCTCTTTTTTGAAATTTATCGGTGGCAGCCGTTAGTATTCGCTGTCCC  >  NC_000913/2168274‑2168569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: