Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,181,282 T→C 5.6% L63L (TTA→CTA) ‡ yegV → putative kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,181,2820TC 5.6% 261.2 / 4.4 92L63L (TTA→CTA) ‡yegVputative kinase
Reads supporting (aligned to +/- strand):  ref base T (47/36);  new base C (2/3);  total (50/42)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

AACCACGCGTCAGTCGGTGATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTGGT  >  NC_000913/2181134‑2181430
                                                                                                                                                    |                                                                                                                                                    
aaCCACGCGTCAGTCGGTGATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGtt                                                                                                                                                     >  2:1587056/1‑150 (MQ=255)
aaCCACGCGTCAGTCGGTGATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGtt                                                                                                                                                     <  2:1334466/150‑1 (MQ=255)
aaCCACGCGTCAGGCGGTGATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGtt                                                                                                                                                     <  2:828828/150‑1 (MQ=255)
   cACGCGTCAGTCGGTGATGGTCGTCGGCGCGGAGGTCATTGACGTGATCGCCGACGCTTATGCCGTCCCCTGGCGTGGGTGAGATATCGAACTGAAACCGAAGAGGCGTAACGTTGGCGGCTGCGACCTGAATATTTCCGTGGCGTGaaa                                                                                                                                                  >  2:1045332/1‑150 (MQ=255)
     cgcgTCAGTCGGTGATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAgc                                                                                                                                                <  2:613173/150‑1 (MQ=255)
       catCAGTCGGTGATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAgcgc                                                                                                                                              >  2:1224131/3‑150 (MQ=255)
         tCAGTCGGTGATGGTCGTCGGCGCGGCGGTCATTGCCGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCt                                                                                                                                            >  2:79267/1‑150 (MQ=255)
          cAGTCGGTGATGGGCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTc                                                                                                                                           <  2:171465/150‑1 (MQ=255)
          cAGTCGGTGATGGGCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTc                                                                                                                                           <  2:1681839/150‑1 (MQ=255)
            gTCGGTGATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCgg                                                                                                                                         >  1:539371/1‑150 (MQ=255)
            gTCGGTGATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCgg                                                                                                                                         >  2:140826/1‑150 (MQ=255)
              cGGTGATGGTCGTCGGCGAGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCAGCGCACTGAATATTACCCTGGCGTTAAAGCGCCTCGGCa                                                                                                                                       >  2:164439/1‑150 (MQ=255)
                 tGATGGTCGGCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGAGGCGCTTTAACGCCACGGCaata                                                                                                                                    <  2:182211/150‑4 (MQ=255)
                 tGATGGGCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCg                                                                                                                                    <  2:858384/150‑1 (MQ=255)
                  gATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGAGGCGCTTTAACGCCACGGCaatat                                                                                                                                   <  1:1679844/150‑5 (MQ=255)
                    tGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGAGGCGCTTTAACGCCACGGCaatattc                                                                                                                                 <  1:416534/150‑7 (MQ=255)
                     ggTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGACGTGGCGTTAAAGCGCCTCGGCATCGAAGc                                                                                                                                >  2:1375546/1‑150 (MQ=255)
                      gtcgtcGGCGCGGCGGCCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGATAAAGCGCCTCGGCATCGAAGCg                                                                                                                               >  1:103871/1‑150 (MQ=255)
                       gcggcggcgcggcgGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCgg                                                                                                                              <  2:620083/146‑1 (MQ=255)
                         gtcgGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGt                                                                                                                            <  1:182018/150‑1 (MQ=255)
                             gcgcggcgGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATg                                                                                                                        <  2:1059773/150‑1 (MQ=255)
                               gcggcgGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGcc                                                                                                                      <  2:1562276/150‑1 (MQ=255)
                                   cggTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGc                                                                                                                  <  1:569838/150‑1 (MQ=255)
                                   cggTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGc                                                                                                                  >  1:377567/1‑150 (MQ=255)
                                       cATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCt                                                                                                              <  1:1100243/150‑1 (MQ=255)
                                        aTTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTc                                                                                                             >  2:1695758/1‑150 (MQ=255)
                                               tGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAgg                                                                                                      <  1:1347428/150‑1 (MQ=255)
                                               tGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAgg                                                                                                      >  2:532165/1‑150 (MQ=255)
                                                gATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAggg                                                                                                     >  1:1546227/1‑150 (MQ=255)
                                                    gCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCgtg                                                                                                 >  1:1361442/1‑150 (MQ=255)
                                                     ccgccgCTGATGCCCTCCCCTGGCGTGGGGGCGAGATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCgtgt                                                                                                <  1:322828/150‑1 (MQ=255)
                                                       gACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTgg                                                                                              <  2:1038940/150‑1 (MQ=255)
                                                        aCGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTggg                                                                                             <  1:1525815/150‑1 (MQ=255)
                                                           cTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCgg                                                                                          >  1:100926/1‑150 (MQ=255)
                                                             tATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGACACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGGTAAAGCGCCGCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGgag                                                                                        <  1:32788/150‑1 (MQ=255)
                                                             tATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGgag                                                                                        >  1:471012/1‑150 (MQ=255)
                                                                gCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAgatg                                                                                     >  2:71226/1‑150 (MQ=255)
                                                                 cccTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCGCGGTCAGGGCGTGTGGGCGGAgatga                                                                                    >  2:152524/1‑150 (MQ=255)
                                                                 cccTCCCCTGGCGTGGGTGCGATATCGAACCGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAgatga                                                                                    >  1:488483/1‑150 (MQ=255)
                                                                   cTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCggg                                                                                                                             <  1:253354/107‑1 (MQ=255)
                                                                   cTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCggg                                                                                                                             >  2:253354/1‑107 (MQ=255)
                                                                   cTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGAtt                                                                                  <  1:1105541/150‑1 (MQ=255)
                                                                   cTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGAtt                                                                                  <  1:668842/150‑1 (MQ=255)
                                                                   cTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGAtt                                                                                  >  2:84630/1‑150 (MQ=255)
                                                                   cTCCCCTGGCGTGGGGGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGAtt                                                                                  <  2:180674/150‑1 (MQ=255)
                                                                    gccccTCGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGGTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTc                                                                                 <  2:323244/149‑1 (MQ=255)
                                                                     ccccTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCg                                                                                <  1:199039/150‑1 (MQ=255)
                                                                      cccTGGCGTGGGTCCGAAATCGAAATGAAAAAGCAGAGTGTAAACTTTGGCGGCTGCGCACGGAATATTGCCTTGGCGTTAAAGCGCATCGGCATCGAAGCGGGTAATGAATTGCCTCTCAGTCAGGCCGCGTGGCCGCAGAGGTTTccc                                                                               >  2:1720938/1‑148 (MQ=255)
                                                                       ccTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGAGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCa                                                                              >  2:23372/1‑150 (MQ=255)
                                                                       ccTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGATGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCa                                                                              >  2:17305/1‑150 (MQ=255)
                                                                           gCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCGGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCATCCg                                                                          <  1:1631617/150‑1 (MQ=255)
                                                                               gggTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCATGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATg                                                                      <  1:593050/150‑1 (MQ=255)
                                                                                   gCGATCTCGAACTGAAACAGCAGAGCGGTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCaa                                                                  <  1:164439/150‑1 (MQ=255)
                                                                                        atCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAagag                                                             >  2:1733260/1‑150 (MQ=255)
                                                                                          cGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAggg                                                           >  2:1626053/1‑150 (MQ=255)
                                                                                           gAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGc                                                          <  2:705763/150‑1 (MQ=255)
                                                                                              cTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTa                                                       <  2:1189661/150‑1 (MQ=255)
                                                                                                gAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAAt                                                     >  1:12670/1‑150 (MQ=255)
                                                                                                gAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAAt                                                     >  2:1665942/1‑150 (MQ=255)
                                                                                                 aaaCAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGGAACCGGAGGGCAAAAGAGGGCTTAATc                                                    >  1:345294/1‑150 (MQ=255)
                                                                                                 aaaCAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATc                                                    <  2:889727/150‑1 (MQ=255)
                                                                                                 aaaCAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGAGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATc                                                    >  1:805517/1‑150 (MQ=255)
                                                                                                   aCAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGTGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAg                                                  <  1:140826/150‑1 (MQ=255)
                                                                                                         gagCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGAt                                            <  1:553627/150‑1 (MQ=255)
                                                                                                         gagCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGGGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGAt                                            >  2:912095/1‑150 (MQ=255)
                                                                                                          agCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATc                                           <  1:1224131/150‑1 (MQ=255)
                                                                                                             gTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGAt                                        >  2:1597222/1‑150 (MQ=255)
                                                                                                              ttAACGTTGGCGGCTGCGCACTGAATATTTCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATa                                       <  1:1549051/150‑1 (MQ=255)
                                                                                                                 aCGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGGTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACg                                    <  1:152524/150‑1 (MQ=255)
                                                                                                                   gTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGcc                                  >  2:1298590/1‑150 (MQ=255)
                                                                                                                   gTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGcc                                  <  2:539371/150‑1 (MQ=255)
                                                                                                                   gTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGcc                                  >  2:564297/1‑150 (MQ=255)
                                                                                                                    ttGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCg                                 >  2:1271240/1‑150 (MQ=255)
                                                                                                                      ggcggcTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGaa                               >  1:1604052/1‑150 (MQ=255)
                                                                                                                      ggcggcTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGaa                               >  2:688500/1‑150 (MQ=255)
                                                                                                                       gcggcTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGAATCGAAGCGGGTAATGCCTTGCCTCTCGGTCAGGGCGTGTGGGCGGATATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAg                              >  2:261011/1‑150 (MQ=255)
                                                                                                                        cggcTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAgg                             >  2:116267/1‑150 (MQ=255)
                                                                                                                             gcgcACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATa                        >  2:64217/1‑150 (MQ=255)
                                                                                                                               gcACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGCGGGCTTAATCAGCCTGATCTATAACGCCGAAGGTGATAAc                      >  2:170952/1‑150 (MQ=255)
                                                                                                                                    gAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTg                 <  1:1597222/150‑1 (MQ=255)
                                                                                                                                    gAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTg                 <  1:1557726/150‑1 (MQ=255)
                                                                                                                                     aaTATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTgg                <  2:1546227/150‑1 (MQ=255)
                                                                                                                                     aaTATAGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTgg                <  2:297262/150‑1 (MQ=255)
                                                                                                                                        gatGCCGAGGCGCTTTAACGCCACGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGgtgt             >  1:518008/3‑150 (MQ=255)
                                                                                                                                        gatGCCGAGGCGCTTTAACGCCACGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGgtgt             >  1:1408430/3‑150 (MQ=255)
                                                                                                                                           gCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTg          >  2:601905/1‑150 (MQ=255)
                                                                                                                                           gCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTg          >  1:924244/1‑150 (MQ=255)
                                                                                                                                           gCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTg          >  2:669993/1‑150 (MQ=255)
                                                                                                                                           gCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGT‑GGCCGAGGTGAATAGCAACCGGATGGCAAAAGAGGGCATAAACAACCAGATCGAAAACCCCGAATGGGAATACGGCTTgtttaagg         >  2:750083/1‑144 (MQ=255)
                                                                                                                                             cGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGgc        <  1:1626053/150‑1 (MQ=255)
                                                                                                                                                  cGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATACCGGCTGGTGTCTGGCGCTgg   >  2:1535061/1‑150 (MQ=255)
                                                                                                                                                  cGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTgg   <  2:645637/150‑1 (MQ=255)
                                                                                                                                                  cGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTCCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTgg   >  2:1145414/1‑150 (MQ=255)
                                                                                                                                                  cGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCATGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGCTAACGCCTAAGGTGATAACGGCTGGGGTCTGGCGCggg   >  2:1535066/1‑150 (MQ=255)
                                                                                                                                                   gTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTGGt  >  1:1545623/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AACCACGCGTCAGTCGGTGATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTGGT  >  NC_000913/2181134‑2181430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑