Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,181,284	A→T	5.3%	L63F (TTA→TTT) ‡	yegV →	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,181,284	0	A	T	5.3%	272.6 / 3.2	92	L63F (TTA→TTT) ‡	yegV	putative kinase
Reads supporting (aligned to +/- strand):  ref base A (48/39);  new base T (2/3);  total (50/42)
Fisher's exact test for biased strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

CACGCGTCAGTCGGTGATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTGGTTG  >  NC_000913/2181137‑2181432                                                                                                                                                    |                                                                                                                                                     cACGCGTCAGTCGGTGATGGTCGTCGGCGCGGAGGTCATTGACGTGATCGCCGACGCTTATGCCGTCCCCTGGCGTGGGTGAGATATCGAACTGAAACCGAAGAGGCGTAACGTTGGCGGCTGCGACCTGAATATTTCCGTGGCGTGaaa                                                                                                                                                    >  2:1045332/1‑150 (MQ=255)   cgcgTCAGTCGGTGATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAgc                                                                                                                                                  <  2:613173/150‑1 (MQ=255)     catCAGTCGGTGATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAgcgc                                                                                                                                                >  2:1224131/3‑150 (MQ=255)       tCAGTCGGTGATGGTCGTCGGCGCGGCGGTCATTGCCGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCt                                                                                                                                              >  2:79267/1‑150 (MQ=255)        cAGTCGGTGATGGGCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTc                                                                                                                                             <  2:171465/150‑1 (MQ=255)        cAGTCGGTGATGGGCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTc                                                                                                                                             <  2:1681839/150‑1 (MQ=255)          gTCGGTGATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCgg                                                                                                                                           >  2:140826/1‑150 (MQ=255)          gTCGGTGATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCgg                                                                                                                                           >  1:539371/1‑150 (MQ=255)            cGGTGATGGTCGTCGGCGAGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCAGCGCACTGAATATTACCCTGGCGTTAAAGCGCCTCGGCa                                                                                                                                         >  2:164439/1‑150 (MQ=255)               tGATGGTCGGCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGAGGCGCTTTAACGCCACGGCaata                                                                                                                                      <  2:182211/150‑4 (MQ=255)               tGATGGGCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCg                                                                                                                                      <  2:858384/150‑1 (MQ=255)                gATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGAGGCGCTTTAACGCCACGGCaatat                                                                                                                                     <  1:1679844/150‑5 (MQ=255)                  tGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGAGGCGCTTTAACGCCACGGCaatattc                                                                                                                                   <  1:416534/150‑7 (MQ=255)                   ggTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGACGTGGCGTTAAAGCGCCTCGGCATCGAAGc                                                                                                                                  >  2:1375546/1‑150 (MQ=255)                    gtcgtcGGCGCGGCGGCCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGATAAAGCGCCTCGGCATCGAAGCg                                                                                                                                 >  1:103871/1‑150 (MQ=255)                     gcggcggcgcggcgGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCgg                                                                                                                                <  2:620083/146‑1 (MQ=255)                       gtcgGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGt                                                                                                                              <  1:182018/150‑1 (MQ=255)                           gcgcggcgGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATg                                                                                                                          <  2:1059773/150‑1 (MQ=255)                             gcggcgGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGcc                                                                                                                        <  2:1562276/150‑1 (MQ=255)                                 cggTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGc                                                                                                                    <  1:569838/150‑1 (MQ=255)                                 cggTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGc                                                                                                                    >  1:377567/1‑150 (MQ=255)                                     cATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCt                                                                                                                <  1:1100243/150‑1 (MQ=255)                                      aTTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTc                                                                                                               >  2:1695758/1‑150 (MQ=255)                                             tGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAgg                                                                                                        <  1:1347428/150‑1 (MQ=255)                                             tGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAgg                                                                                                        >  2:532165/1‑150 (MQ=255)                                              gATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAggg                                                                                                       >  1:1546227/1‑150 (MQ=255)                                                  gCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCgtg                                                                                                   >  1:1361442/1‑150 (MQ=255)                                                   ccgccgCTGATGCCCTCCCCTGGCGTGGGGGCGAGATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCgtgt                                                                                                  <  1:322828/150‑1 (MQ=255)                                                     gACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTgg                                                                                                <  2:1038940/150‑1 (MQ=255)                                                      aCGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTggg                                                                                               <  1:1525815/150‑1 (MQ=255)                                                         cTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCgg                                                                                            >  1:100926/1‑150 (MQ=255)                                                           tATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGACACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGGTAAAGCGCCGCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGgag                                                                                          <  1:32788/150‑1 (MQ=255)                                                           tATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGgag                                                                                          >  1:471012/1‑150 (MQ=255)                                                              gCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAgatg                                                                                       >  2:71226/1‑150 (MQ=255)                                                               cccTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCGCGGTCAGGGCGTGTGGGCGGAgatga                                                                                      >  2:152524/1‑150 (MQ=255)                                                               cccTCCCCTGGCGTGGGTGCGATATCGAACCGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAgatga                                                                                      >  1:488483/1‑150 (MQ=255)                                                                 cTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCggg                                                                                                                               >  2:253354/1‑107 (MQ=255)                                                                 cTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCggg                                                                                                                               <  1:253354/107‑1 (MQ=255)                                                                 cTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGAtt                                                                                    <  1:1105541/150‑1 (MQ=255)                                                                 cTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGAtt                                                                                    <  1:668842/150‑1 (MQ=255)                                                                 cTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGAtt                                                                                    >  2:84630/1‑150 (MQ=255)                                                                 cTCCCCTGGCGTGGGGGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGAtt                                                                                    <  2:180674/150‑1 (MQ=255)                                                                  gccccTCGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGGTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTc                                                                                   <  2:323244/149‑1 (MQ=255)                                                                   ccccTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCg                                                                                  <  1:199039/150‑1 (MQ=255)                                                                    cccTGGCGTGGGTCCGAAATCGAAATGAAAAAGCAGAGTGTAAACTTTGGCGGCTGCGCACGGAATATTGCCTTGGCGTTAAAGCGCATCGGCATCGAAGCGGGTAATGAATTGCCTCTCAGTCAGGCCGCGTGGCCGCAGAGGTTTccc                                                                                 >  2:1720938/1‑148 (MQ=255)                                                                     ccTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGAGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCa                                                                                >  2:23372/1‑150 (MQ=255)                                                                     ccTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGATGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCa                                                                                >  2:17305/1‑150 (MQ=255)                                                                         gCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCGGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCATCCg                                                                            <  1:1631617/150‑1 (MQ=255)                                                                             gggTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCATGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATg                                                                        <  1:593050/150‑1 (MQ=255)                                                                                 gCGATCTCGAACTGAAACAGCAGAGCGGTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCaa                                                                    <  1:164439/150‑1 (MQ=255)                                                                                      atCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAagag                                                               >  2:1733260/1‑150 (MQ=255)                                                                                        cGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAggg                                                             >  2:1626053/1‑150 (MQ=255)                                                                                         gAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGc                                                            <  2:705763/150‑1 (MQ=255)                                                                                            cTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTa                                                         <  2:1189661/150‑1 (MQ=255)                                                                                              gAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAAt                                                       >  1:12670/1‑150 (MQ=255)                                                                                              gAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAAt                                                       >  2:1665942/1‑150 (MQ=255)                                                                                               aaaCAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGGAACCGGAGGGCAAAAGAGGGCTTAATc                                                      >  1:345294/1‑150 (MQ=255)                                                                                               aaaCAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATc                                                      <  2:889727/150‑1 (MQ=255)                                                                                               aaaCAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGAGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATc                                                      >  1:805517/1‑150 (MQ=255)                                                                                                 aCAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGTGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAg                                                    <  1:140826/150‑1 (MQ=255)                                                                                                       gagCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGAt                                              <  1:553627/150‑1 (MQ=255)                                                                                                       gagCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGGGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGAt                                              >  2:912095/1‑150 (MQ=255)                                                                                                        agCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATc                                             <  1:1224131/150‑1 (MQ=255)                                                                                                           gTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGAt                                          >  2:1597222/1‑150 (MQ=255)                                                                                                            ttAACGTTGGCGGCTGCGCACTGAATATTTCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATa                                         <  1:1549051/150‑1 (MQ=255)                                                                                                               aCGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGGTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACg                                      <  1:152524/150‑1 (MQ=255)                                                                                                                 gTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGcc                                    >  2:1298590/1‑150 (MQ=255)                                                                                                                 gTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGcc                                    <  2:539371/150‑1 (MQ=255)                                                                                                                 gTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGcc                                    >  2:564297/1‑150 (MQ=255)                                                                                                                  ttGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCg                                   >  2:1271240/1‑150 (MQ=255)                                                                                                                    ggcggcTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGaa                                 >  2:688500/1‑150 (MQ=255)                                                                                                                    ggcggcTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGaa                                 >  1:1604052/1‑150 (MQ=255)                                                                                                                     gcggcTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGAATCGAAGCGGGTAATGCCTTGCCTCTCGGTCAGGGCGTGTGGGCGGATATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAg                                >  2:261011/1‑150 (MQ=255)                                                                                                                      cggcTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAgg                               >  2:116267/1‑150 (MQ=255)                                                                                                                           gcgcACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATa                          >  2:64217/1‑150 (MQ=255)                                                                                                                             gcACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGCGGGCTTAATCAGCCTGATCTATAACGCCGAAGGTGATAAc                        >  2:170952/1‑150 (MQ=255)                                                                                                                                  gAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTg                   <  1:1597222/150‑1 (MQ=255)                                                                                                                                  gAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTg                   <  1:1557726/150‑1 (MQ=255)                                                                                                                                   aaTATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTgg                  <  2:1546227/150‑1 (MQ=255)                                                                                                                                   aaTATAGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTgg                  <  2:297262/150‑1 (MQ=255)                                                                                                                                      gatGCCGAGGCGCTTTAACGCCACGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGgtgt               >  1:1408430/3‑150 (MQ=255)                                                                                                                                      gatGCCGAGGCGCTTTAACGCCACGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGgtgt               >  1:518008/3‑150 (MQ=255)                                                                                                                                         gCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTg            >  2:601905/1‑150 (MQ=255)                                                                                                                                         gCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTg            >  2:669993/1‑150 (MQ=255)                                                                                                                                         gCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTg            >  1:924244/1‑150 (MQ=255)                                                                                                                                         gCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGT‑GGCCGAGGTGAATAGCAACCGGATGGCAAAAGAGGGCATAAACAACCAGATCGAAAACCCCGAATGGGAATACGGCTTgtttaagg           >  2:750083/1‑144 (MQ=255)                                                                                                                                           cGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGgc          <  1:1626053/150‑1 (MQ=255)                                                                                                                                                cGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATACCGGCTGGTGTCTGGCGCTgg     >  2:1535061/1‑150 (MQ=255)                                                                                                                                                cGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTgg     <  2:645637/150‑1 (MQ=255)                                                                                                                                                cGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTCCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTgg     >  2:1145414/1‑150 (MQ=255)                                                                                                                                                cGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCATGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGCTAACGCCTAAGGTGATAACGGCTGGGGTCTGGCGCggg     >  2:1535066/1‑150 (MQ=255)                                                                                                                                                 gTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTGGt    >  1:1545623/1‑150 (MQ=255)                                                                                                                                                   tAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTGGTTg  >  2:768082/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                     CACGCGTCAGTCGGTGATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTGGTTG  >  NC_000913/2181137‑2181432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑