Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,181,287 G→A 5.3% K64K (AAG→AAA) ‡ yegV → putative kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,181,2870GA 5.3% 298.5 / 3.2 93K64K (AAG→AAA) ‡yegVputative kinase
Reads supporting (aligned to +/- strand):  ref base G (48/40);  new base A (2/3);  total (50/43)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGTCAGTCGGTGATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTGGTTGAGCC  >  NC_000913/2181139‑2181436
                                                                                                                                                    |                                                                                                                                                     
cgcgTCAGTCGGTGATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAgc                                                                                                                                                      <  2:613173/150‑1 (MQ=255)
  catCAGTCGGTGATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAgcgc                                                                                                                                                    >  2:1224131/3‑150 (MQ=255)
    tCAGTCGGTGATGGTCGTCGGCGCGGCGGTCATTGCCGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCt                                                                                                                                                  >  2:79267/1‑150 (MQ=255)
     cAGTCGGTGATGGGCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTc                                                                                                                                                 <  2:171465/150‑1 (MQ=255)
     cAGTCGGTGATGGGCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTc                                                                                                                                                 <  2:1681839/150‑1 (MQ=255)
       gTCGGTGATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCgg                                                                                                                                               >  2:140826/1‑150 (MQ=255)
       gTCGGTGATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCgg                                                                                                                                               >  1:539371/1‑150 (MQ=255)
         cGGTGATGGTCGTCGGCGAGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCAGCGCACTGAATATTACCCTGGCGTTAAAGCGCCTCGGCa                                                                                                                                             >  2:164439/1‑150 (MQ=255)
            tGATGGTCGGCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGAGGCGCTTTAACGCCACGGCaata                                                                                                                                          <  2:182211/150‑4 (MQ=255)
            tGATGGGCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCg                                                                                                                                          <  2:858384/150‑1 (MQ=255)
             gATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGAGGCGCTTTAACGCCACGGCaatat                                                                                                                                         <  1:1679844/150‑5 (MQ=255)
               tGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGAGGCGCTTTAACGCCACGGCaatattc                                                                                                                                       <  1:416534/150‑7 (MQ=255)
                ggTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGACGTGGCGTTAAAGCGCCTCGGCATCGAAGc                                                                                                                                      >  2:1375546/1‑150 (MQ=255)
                 gtcgtcGGCGCGGCGGCCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGATAAAGCGCCTCGGCATCGAAGCg                                                                                                                                     >  1:103871/1‑150 (MQ=255)
                  gcggcggcgcggcgGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCgg                                                                                                                                    <  2:620083/146‑1 (MQ=255)
                    gtcgGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGt                                                                                                                                  <  1:182018/150‑1 (MQ=255)
                        gcgcggcgGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATg                                                                                                                              <  2:1059773/150‑1 (MQ=255)
                          gcggcgGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGcc                                                                                                                            <  2:1562276/150‑1 (MQ=255)
                              cggTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGc                                                                                                                        <  1:569838/150‑1 (MQ=255)
                              cggTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGc                                                                                                                        >  1:377567/1‑150 (MQ=255)
                                  cATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCt                                                                                                                    <  1:1100243/150‑1 (MQ=255)
                                   aTTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTc                                                                                                                   >  2:1695758/1‑150 (MQ=255)
                                          tGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAgg                                                                                                            <  1:1347428/150‑1 (MQ=255)
                                          tGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAgg                                                                                                            >  2:532165/1‑150 (MQ=255)
                                           gATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAggg                                                                                                           >  1:1546227/1‑150 (MQ=255)
                                               gCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCgtg                                                                                                       >  1:1361442/1‑150 (MQ=255)
                                                ccgccgCTGATGCCCTCCCCTGGCGTGGGGGCGAGATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCgtgt                                                                                                      <  1:322828/150‑1 (MQ=255)
                                                  gACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTgg                                                                                                    <  2:1038940/150‑1 (MQ=255)
                                                   aCGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTggg                                                                                                   <  1:1525815/150‑1 (MQ=255)
                                                      cTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCgg                                                                                                >  1:100926/1‑150 (MQ=255)
                                                        tATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGACACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGGTAAAGCGCCGCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGgag                                                                                              <  1:32788/150‑1 (MQ=255)
                                                        tATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGgag                                                                                              >  1:471012/1‑150 (MQ=255)
                                                           gCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAgatg                                                                                           >  2:71226/1‑150 (MQ=255)
                                                            cccTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCGCGGTCAGGGCGTGTGGGCGGAgatga                                                                                          >  2:152524/1‑150 (MQ=255)
                                                            cccTCCCCTGGCGTGGGTGCGATATCGAACCGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAgatga                                                                                          >  1:488483/1‑150 (MQ=255)
                                                              cTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCggg                                                                                                                                   <  1:253354/107‑1 (MQ=255)
                                                              cTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCggg                                                                                                                                   >  2:253354/1‑107 (MQ=255)
                                                              cTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGAtt                                                                                        <  1:1105541/150‑1 (MQ=255)
                                                              cTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGAtt                                                                                        <  1:668842/150‑1 (MQ=255)
                                                              cTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGAtt                                                                                        >  2:84630/1‑150 (MQ=255)
                                                              cTCCCCTGGCGTGGGGGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGAtt                                                                                        <  2:180674/150‑1 (MQ=255)
                                                               gccccTCGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGGTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTc                                                                                       <  2:323244/149‑1 (MQ=255)
                                                                ccccTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCg                                                                                      <  1:199039/150‑1 (MQ=255)
                                                                 cccTGGCGTGGGTCCGAAATCGAAATGAAAAAGCAGAGTGTAAACTTTGGCGGCTGCGCACGGAATATTGCCTTGGCGTTAAAGCGCATCGGCATCGAAGCGGGTAATGAATTGCCTCTCAGTCAGGCCGCGTGGCCGCAGAGGTTTccc                                                                                     >  2:1720938/1‑148 (MQ=255)
                                                                  ccTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGAGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCa                                                                                    >  2:23372/1‑150 (MQ=255)
                                                                  ccTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGATGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCa                                                                                    >  2:17305/1‑150 (MQ=255)
                                                                      gCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCGGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCATCCg                                                                                <  1:1631617/150‑1 (MQ=255)
                                                                          gggTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCATGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATg                                                                            <  1:593050/150‑1 (MQ=255)
                                                                              gCGATCTCGAACTGAAACAGCAGAGCGGTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCaa                                                                        <  1:164439/150‑1 (MQ=255)
                                                                                   atCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAagag                                                                   >  2:1733260/1‑150 (MQ=255)
                                                                                     cGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAggg                                                                 >  2:1626053/1‑150 (MQ=255)
                                                                                      gAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGc                                                                <  2:705763/150‑1 (MQ=255)
                                                                                         cTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTa                                                             <  2:1189661/150‑1 (MQ=255)
                                                                                           gAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAAt                                                           >  1:12670/1‑150 (MQ=255)
                                                                                           gAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAAt                                                           >  2:1665942/1‑150 (MQ=255)
                                                                                            aaaCAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGGAACCGGAGGGCAAAAGAGGGCTTAATc                                                          >  1:345294/1‑150 (MQ=255)
                                                                                            aaaCAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATc                                                          <  2:889727/150‑1 (MQ=255)
                                                                                            aaaCAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGAGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATc                                                          >  1:805517/1‑150 (MQ=255)
                                                                                              aCAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGTGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAg                                                        <  1:140826/150‑1 (MQ=255)
                                                                                                    gagCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGAt                                                  <  1:553627/150‑1 (MQ=255)
                                                                                                    gagCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGGGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGAt                                                  >  2:912095/1‑150 (MQ=255)
                                                                                                     agCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATc                                                 <  1:1224131/150‑1 (MQ=255)
                                                                                                        gTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGAt                                              >  2:1597222/1‑150 (MQ=255)
                                                                                                         ttAACGTTGGCGGCTGCGCACTGAATATTTCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATa                                             <  1:1549051/150‑1 (MQ=255)
                                                                                                            aCGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGGTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACg                                          <  1:152524/150‑1 (MQ=255)
                                                                                                              gTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGcc                                        <  2:539371/150‑1 (MQ=255)
                                                                                                              gTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGcc                                        >  2:564297/1‑150 (MQ=255)
                                                                                                              gTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGcc                                        >  2:1298590/1‑150 (MQ=255)
                                                                                                               ttGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCg                                       >  2:1271240/1‑150 (MQ=255)
                                                                                                                 ggcggcTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGaa                                     >  2:688500/1‑150 (MQ=255)
                                                                                                                 ggcggcTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGaa                                     >  1:1604052/1‑150 (MQ=255)
                                                                                                                  gcggcTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGAATCGAAGCGGGTAATGCCTTGCCTCTCGGTCAGGGCGTGTGGGCGGATATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAg                                    >  2:261011/1‑150 (MQ=255)
                                                                                                                   cggcTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAgg                                   >  2:116267/1‑150 (MQ=255)
                                                                                                                        gcgcACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATa                              >  2:64217/1‑150 (MQ=255)
                                                                                                                          gcACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGCGGGCTTAATCAGCCTGATCTATAACGCCGAAGGTGATAAc                            >  2:170952/1‑150 (MQ=255)
                                                                                                                               gAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTg                       <  1:1557726/150‑1 (MQ=255)
                                                                                                                               gAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTg                       <  1:1597222/150‑1 (MQ=255)
                                                                                                                                aaTATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTgg                      <  2:1546227/150‑1 (MQ=255)
                                                                                                                                aaTATAGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTgg                      <  2:297262/150‑1 (MQ=255)
                                                                                                                                   gatGCCGAGGCGCTTTAACGCCACGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGgtgt                   >  1:518008/3‑150 (MQ=255)
                                                                                                                                   gatGCCGAGGCGCTTTAACGCCACGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGgtgt                   >  1:1408430/3‑150 (MQ=255)
                                                                                                                                      gCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTg                >  1:924244/1‑150 (MQ=255)
                                                                                                                                      gCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTg                >  2:669993/1‑150 (MQ=255)
                                                                                                                                      gCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTg                >  2:601905/1‑150 (MQ=255)
                                                                                                                                      gCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGT‑GGCCGAGGTGAATAGCAACCGGATGGCAAAAGAGGGCATAAACAACCAGATCGAAAACCCCGAATGGGAATACGGCTTgtttaagg               >  2:750083/1‑144 (MQ=255)
                                                                                                                                        cGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGgc              <  1:1626053/150‑1 (MQ=255)
                                                                                                                                             cGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATACCGGCTGGTGTCTGGCGCTgg         >  2:1535061/1‑150 (MQ=255)
                                                                                                                                             cGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTgg         <  2:645637/150‑1 (MQ=255)
                                                                                                                                             cGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTCCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTgg         >  2:1145414/1‑150 (MQ=255)
                                                                                                                                             cGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCATGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGCTAACGCCTAAGGTGATAACGGCTGGGGTCTGGCGCggg         >  2:1535066/1‑150 (MQ=255)
                                                                                                                                              gTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTGGt        >  1:1545623/1‑150 (MQ=255)
                                                                                                                                                tAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTGGTTg      >  2:768082/1‑150 (MQ=255)
                                                                                                                                                  aaGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTGGTTGAg    >  1:1630492/1‑150 (MQ=255)
                                                                                                                                                  aaGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTGGTTGAg    <  1:1587056/150‑1 (MQ=255)
                                                                                                                                                   aGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTGGTTGAGc   <  2:1415664/150‑1 (MQ=255)
                                                                                                                                                    gcgcCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTGGTTGAGcc  >  1:718740/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGCGTCAGTCGGTGATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTGGTTGAGCC  >  NC_000913/2181139‑2181436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑