Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,181,292 T→A 5.3% L66H (CTC→CAC)  yegV → putative kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,181,2920TA 5.3% 254.9 / 3.9 94L66H (CTC→CAC) yegVputative kinase
Reads supporting (aligned to +/- strand):  ref base T (49/39);  new base A (2/3);  total (51/43)
Fisher's exact test for biased strand distribution p-value = 6.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.71e-01

TCAGTCGGTGATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTGGTTGAGCCG  >  NC_000913/2181143‑2181437
                                                                                                                                                     |                                                                                                                                                 
tCAGTCGGTGATGGTCGTCGGCGCGGCGGTCATTGCCGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCt                                                                                                                                                   >  2:79267/1‑150 (MQ=255)
 cAGTCGGTGATGGGCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTc                                                                                                                                                  <  2:1681839/150‑1 (MQ=255)
 cAGTCGGTGATGGGCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTc                                                                                                                                                  <  2:171465/150‑1 (MQ=255)
   gTCGGTGATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCgg                                                                                                                                                >  2:140826/1‑150 (MQ=255)
   gTCGGTGATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCgg                                                                                                                                                >  1:539371/1‑150 (MQ=255)
     cGGTGATGGTCGTCGGCGAGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCAGCGCACTGAATATTACCCTGGCGTTAAAGCGCCTCGGCa                                                                                                                                              >  2:164439/1‑150 (MQ=255)
        tGATGGTCGGCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGAGGCGCTTTAACGCCACGGCaata                                                                                                                                           <  2:182211/150‑4 (MQ=255)
        tGATGGGCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCg                                                                                                                                           <  2:858384/150‑1 (MQ=255)
         gATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGAGGCGCTTTAACGCCACGGCaatat                                                                                                                                          <  1:1679844/150‑5 (MQ=255)
           tGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGAGGCGCTTTAACGCCACGGCaatattc                                                                                                                                        <  1:416534/150‑7 (MQ=255)
            ggTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGACGTGGCGTTAAAGCGCCTCGGCATCGAAGc                                                                                                                                       >  2:1375546/1‑150 (MQ=255)
             gtcgtcGGCGCGGCGGCCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGATAAAGCGCCTCGGCATCGAAGCg                                                                                                                                      >  1:103871/1‑150 (MQ=255)
              gcggcggcgcggcgGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCgg                                                                                                                                     <  2:620083/146‑1 (MQ=255)
                gtcgGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGt                                                                                                                                   <  1:182018/150‑1 (MQ=255)
                    gcgcggcgGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATg                                                                                                                               <  2:1059773/150‑1 (MQ=255)
                      gcggcgGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGcc                                                                                                                             <  2:1562276/150‑1 (MQ=255)
                          cggTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGc                                                                                                                         <  1:569838/150‑1 (MQ=255)
                          cggTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGc                                                                                                                         >  1:377567/1‑150 (MQ=255)
                              cATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCt                                                                                                                     <  1:1100243/150‑1 (MQ=255)
                               aTTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTc                                                                                                                    >  2:1695758/1‑150 (MQ=255)
                                      tGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAgg                                                                                                             <  1:1347428/150‑1 (MQ=255)
                                      tGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAgg                                                                                                             >  2:532165/1‑150 (MQ=255)
                                       gATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAggg                                                                                                            >  1:1546227/1‑150 (MQ=255)
                                           gCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCgtg                                                                                                        >  1:1361442/1‑150 (MQ=255)
                                            ccgccgCTGATGCCCTCCCCTGGCGTGGGGGCGAGATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCgtgt                                                                                                       <  1:322828/150‑1 (MQ=255)
                                              gACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTgg                                                                                                     <  2:1038940/150‑1 (MQ=255)
                                               aCGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTggg                                                                                                    <  1:1525815/150‑1 (MQ=255)
                                                  cTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCgg                                                                                                 >  1:100926/1‑150 (MQ=255)
                                                    tATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGACACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGGTAAAGCGCCGCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGgag                                                                                               <  1:32788/150‑1 (MQ=255)
                                                    tATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGgag                                                                                               >  1:471012/1‑150 (MQ=255)
                                                       gCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAgatg                                                                                            >  2:71226/1‑150 (MQ=255)
                                                        cccTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCGCGGTCAGGGCGTGTGGGCGGAgatga                                                                                           >  2:152524/1‑150 (MQ=255)
                                                        cccTCCCCTGGCGTGGGTGCGATATCGAACCGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAgatga                                                                                           >  1:488483/1‑150 (MQ=255)
                                                          cTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCggg                                                                                                                                    <  1:253354/107‑1 (MQ=255)
                                                          cTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCggg                                                                                                                                    >  2:253354/1‑107 (MQ=255)
                                                          cTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGAtt                                                                                         <  1:668842/150‑1 (MQ=255)
                                                          cTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGAtt                                                                                         >  2:84630/1‑150 (MQ=255)
                                                          cTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGAtt                                                                                         <  1:1105541/150‑1 (MQ=255)
                                                          cTCCCCTGGCGTGGGGGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGAtt                                                                                         <  2:180674/150‑1 (MQ=255)
                                                           gccccTCGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGGTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTc                                                                                        <  2:323244/149‑1 (MQ=255)
                                                            ccccTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCg                                                                                       <  1:199039/150‑1 (MQ=255)
                                                             cccTGGCGTGGGTCCGAAATCGAAATGAAAAAGCAGAGTGTAAACTTTGGCGGCTGCGCACGGAATATTGCCTTGGCGTTAAAGCGCATCGGCATCGAAGCGGGTAATGAATTGCCTCTCAGTCAGGCCGCGTGGCCGCAGAGGTTTccc                                                                                      >  2:1720938/1‑148 (MQ=255)
                                                              ccTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGAGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCa                                                                                     >  2:23372/1‑150 (MQ=255)
                                                              ccTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGATGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCa                                                                                     >  2:17305/1‑150 (MQ=255)
                                                                  gCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCGGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCATCCg                                                                                 <  1:1631617/150‑1 (MQ=255)
                                                                      gggTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCATGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATg                                                                             <  1:593050/150‑1 (MQ=255)
                                                                          gCGATCTCGAACTGAAACAGCAGAGCGGTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCaa                                                                         <  1:164439/150‑1 (MQ=255)
                                                                               atCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAagag                                                                    >  2:1733260/1‑150 (MQ=255)
                                                                                 cGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAggg                                                                  >  2:1626053/1‑150 (MQ=255)
                                                                                  gAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGc                                                                 <  2:705763/150‑1 (MQ=255)
                                                                                     cTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTa                                                              <  2:1189661/150‑1 (MQ=255)
                                                                                       gAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAAt                                                            >  1:12670/1‑150 (MQ=255)
                                                                                       gAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAAt                                                            >  2:1665942/1‑150 (MQ=255)
                                                                                        aaaCAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGGAACCGGAGGGCAAAAGAGGGCTTAATc                                                           >  1:345294/1‑150 (MQ=255)
                                                                                        aaaCAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATc                                                           <  2:889727/150‑1 (MQ=255)
                                                                                        aaaCAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGAGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATc                                                           >  1:805517/1‑150 (MQ=255)
                                                                                          aCAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGTGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAg                                                         <  1:140826/150‑1 (MQ=255)
                                                                                                gagCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGAt                                                   <  1:553627/150‑1 (MQ=255)
                                                                                                gagCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGGGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGAt                                                   >  2:912095/1‑150 (MQ=255)
                                                                                                 agCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATc                                                  <  1:1224131/150‑1 (MQ=255)
                                                                                                    gTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGAt                                               >  2:1597222/1‑150 (MQ=255)
                                                                                                     ttAACGTTGGCGGCTGCGCACTGAATATTTCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATa                                              <  1:1549051/150‑1 (MQ=255)
                                                                                                        aCGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGGTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACg                                           <  1:152524/150‑1 (MQ=255)
                                                                                                          gTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGcc                                         >  2:1298590/1‑150 (MQ=255)
                                                                                                          gTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGcc                                         >  2:564297/1‑150 (MQ=255)
                                                                                                          gTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGcc                                         <  2:539371/150‑1 (MQ=255)
                                                                                                           ttGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCg                                        >  2:1271240/1‑150 (MQ=255)
                                                                                                             ggcggcTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGaa                                      >  1:1604052/1‑150 (MQ=255)
                                                                                                             ggcggcTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGaa                                      >  2:688500/1‑150 (MQ=255)
                                                                                                              gcggcTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGAATCGAAGCGGGTAATGCCTTGCCTCTCGGTCAGGGCGTGTGGGCGGATATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAg                                     >  2:261011/1‑150 (MQ=255)
                                                                                                               cggcTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAgg                                    >  2:116267/1‑150 (MQ=255)
                                                                                                                    gcgcACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATa                               >  2:64217/1‑150 (MQ=255)
                                                                                                                      gcACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGCGGGCTTAATCAGCCTGATCTATAACGCCGAAGGTGATAAc                             >  2:170952/1‑150 (MQ=255)
                                                                                                                           gAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTg                        <  1:1557726/150‑1 (MQ=255)
                                                                                                                           gAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTg                        <  1:1597222/150‑1 (MQ=255)
                                                                                                                            aaTATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTgg                       <  2:1546227/150‑1 (MQ=255)
                                                                                                                            aaTATAGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTgg                       <  2:297262/150‑1 (MQ=255)
                                                                                                                               gatGCCGAGGCGCTTTAACGCCACGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGgtgt                    >  1:1408430/3‑150 (MQ=255)
                                                                                                                               gatGCCGAGGCGCTTTAACGCCACGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGgtgt                    >  1:518008/3‑150 (MQ=255)
                                                                                                                                  gCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTg                 >  1:924244/1‑150 (MQ=255)
                                                                                                                                  gCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTg                 >  2:601905/1‑150 (MQ=255)
                                                                                                                                  gCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTg                 >  2:669993/1‑150 (MQ=255)
                                                                                                                                  gCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGT‑GGCCGAGGTGAATAGCAACCGGATGGCAAAAGAGGGCATAAACAACCAGATCGAAAACCCCGAATGGGAATACGGCTTgtttaagg                >  2:750083/1‑144 (MQ=255)
                                                                                                                                    cGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGgc               <  1:1626053/150‑1 (MQ=255)
                                                                                                                                         cGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATACCGGCTGGTGTCTGGCGCTgg          >  2:1535061/1‑150 (MQ=255)
                                                                                                                                         cGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTgg          <  2:645637/150‑1 (MQ=255)
                                                                                                                                         cGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTCCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTgg          >  2:1145414/1‑150 (MQ=255)
                                                                                                                                         cGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCATGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGCTAACGCCTAAGGTGATAACGGCTGGGGTCTGGCGCggg          >  2:1535066/1‑150 (MQ=255)
                                                                                                                                          gTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTGGt         >  1:1545623/1‑150 (MQ=255)
                                                                                                                                            tAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTGGTTg       >  2:768082/1‑150 (MQ=255)
                                                                                                                                              aaGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTGGTTGAg     <  1:1587056/150‑1 (MQ=255)
                                                                                                                                              aaGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTGGTTGAg     >  1:1630492/1‑150 (MQ=255)
                                                                                                                                               aGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTGGTTGAGc    <  2:1415664/150‑1 (MQ=255)
                                                                                                                                                gcgcCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTGGTTGAGcc   >  1:718740/1‑150 (MQ=255)
                                                                                                                                                 cgcCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTGGTTGAGCCg  >  1:334028/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TCAGTCGGTGATGGTCGTCGGCGCGGCGGTCATTGACGTGATCGCCGACGCTTATGCCCTCCCCTGGCGTGGGTGCGATATCGAACTGAAACAGCAGAGCGTTAACGTTGGCGGCTGCGCACTGAATATTGCCGTGGCGTTAAAGCGCCTCGGCATCGAAGCGGGTAATGCCTTGCCGCTCGGTCAGGGCGTGTGGGCGGAGATGATTCGCAACCGGATGGCAAAAGAGGGCTTAATCAGCCTGATCGATAACGCCGAAGGTGATAACGGCTGGTGTCTGGCGCTGGTTGAGCCG  >  NC_000913/2181143‑2181437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑