Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,211,186 G→C 6.5% intergenic (+86/‑39) yehQ → / → yehR pseudogene/lipoprotein, DUF1307 family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,211,1860GC 6.5% 349.3 / 9.9 105intergenic (+86/‑39)yehQ/yehRpseudogene/lipoprotein, DUF1307 family
Reads supporting (aligned to +/- strand):  ref base G (56/42);  new base C (2/5);  total (58/47)
Fisher's exact test for biased strand distribution p-value = 2.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01

Only 100 of 110 total aligned reads displayed.

AGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCGAAGAAATTCAGCGCCAATCTGAAC  >  NC_000913/2211037‑2211335
                                                                                                                                                     |                                                                                                                                                     
aGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTgag                                                                                                                                                       >  1:424257/1‑150 (MQ=255)
 gCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGAATTAGGCATATCCTATATAGCCCCTGGTgaga                                                                                                                                                      >  2:929607/1‑150 (MQ=255)
  cAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGTACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGagag                                                                                                                                                     >  2:1263051/1‑150 (MQ=255)
     tGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCa                                                                                                                                                  >  2:927414/1‑150 (MQ=255)
      gAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCAc                                                                                                                                                 >  1:408256/1‑150 (MQ=255)
       aaTAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCAcc                                                                                                                                                <  2:1291154/150‑1 (MQ=255)
       aaTAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCAcc                                                                                                                                                <  1:678686/150‑1 (MQ=255)
           aCGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGACTCTCACCAggg                                                                                                                                            <  2:411571/150‑3 (MQ=255)
           aCGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGACTCTCACCAggg                                                                                                                                            <  1:215324/150‑3 (MQ=255)
            cGGTGTTTTTCTTTGCGAACAAATAATCCCCATTCTTCAGCAACAAGGATGAAATAGTGCTTATTACTAAGAGATCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGACTCTCACCAgggg                                                                                                                                           <  1:1694369/150‑4 (MQ=255)
             ggTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGACTCTCACCAggggc                                                                                                                                          <  2:1723039/150‑5 (MQ=255)
                gtTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTg                                                                                                                                       >  1:1092798/1‑150 (MQ=255)
                gtTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTg                                                                                                                                       <  2:1579213/150‑1 (MQ=255)
                     gctttgcGAACAATTATTCCCCATGCTTCAGCAACAGGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATtaa                                                                                                                                  >  1:1695425/1‑150 (MQ=255)
                     gctttgcGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGAGCTTTTTACTAAGAGTTCTACTCAAGTTACGGCCTGCTCACGCCACGGTATTAGGCATATGCGTTATAGCCCCTGGTGTGGGTGACCAGTTCCTTGATtaa                                                                                                                                  >  2:112039/1‑150 (MQ=255)
                        ttgcGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATtaaata                                                                                                                               >  1:774183/1‑150 (MQ=255)
                            gAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAAt                                                                                                                           >  1:1506178/1‑150 (MQ=255)
                               cAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCGCGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGa                                                                                                                        <  2:1173400/150‑1 (MQ=255)
                               cAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGa                                                                                                                        >  1:1722177/1‑150 (MQ=255)
                               cAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGAGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACAGCTCACGCCACGGTATTAGTCATATCCTATATAGCCCCTGGTGAGAGACACAACTTCCTAGATTAAATAAAATGGa                                                                                                                        >  1:250803/1‑150 (MQ=255)
                                aaTTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAg                                                                                                                       >  2:173298/1‑150 (MQ=255)
                                    attCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGtttt                                                                                                                   >  2:559802/1‑150 (MQ=255)
                                    attCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGtttt                                                                                                                   <  2:472048/150‑1 (MQ=255)
                                      tCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTAc                                                                                                                 <  1:371858/150‑1 (MQ=255)
                                       ccccATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACa                                                                                                                >  2:1677054/1‑150 (MQ=255)
                                       ccccATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACa                                                                                                                <  1:1216811/150‑1 (MQ=255)
                                       ccccATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACa                                                                                                                >  2:182940/1‑150 (MQ=255)
                                        cccATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACAt                                                                                                               >  1:436066/1‑150 (MQ=255)
                                         ccATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATg                                                                                                              >  1:646127/1‑150 (MQ=255)
                                         ccATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCAACGGTATTAGGCATATCCTATATAGCCACTGGTGAGAGTCAACAGTTCCTTGATAAAATAAAATGGAGTTTTACATg                                                                                                              >  1:1111783/1‑150 (MQ=255)
                                         ccATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTACAGTTACGGACTGATCACGCCACGGTATTAGGCATATCATATATAGCCCCTGGTGAGAGTCACAAGTTACTTGATTAAATAAAATGGAGTTTTAAAtt                                                                                                              >  1:1437444/1‑149 (MQ=255)
                                              cTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGc                                                                                                         >  1:953314/1‑150 (MQ=255)
                                              cTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGGGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGc                                                                                                         <  2:1271547/150‑1 (MQ=255)
                                               ttCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGCCTGCTGACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCt                                                                                                        >  2:299008/1‑150 (MQ=255)
                                                 cAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCttt                                                                                                      >  1:41625/1‑150 (MQ=255)
                                                  aGCAACAAGGATGAAATAGTGCTTTTTACTAATTGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTc                                                                                                     <  1:1701323/150‑1 (MQ=255)
                                                     aacaaGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATGCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTTAATAAAATGGAGTTTTACATGAAGGCTTTCAAt                                                                                                  >  2:105363/1‑150 (MQ=255)
                                                     aacaaGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGACACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAAt                                                                                                  >  2:1338790/1‑150 (MQ=255)
                                                        aaGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAGATGGAGTTTTACATGAAGGCTTTCAATAAg                                                                                               <  1:1025714/150‑1 (MQ=255)
                                                        aaGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAg                                                                                               <  1:1492356/150‑1 (MQ=255)
                                                        aaGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAg                                                                                               <  2:1496424/150‑1 (MQ=255)
                                                         aGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTATGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATAGAGTTTTACATGAAGGCTTTCAATAAGc                                                                                              <  1:733230/150‑1 (MQ=255)
                                                         aGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGc                                                                                              <  2:1550761/150‑1 (MQ=255)
                                                          ggATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCt                                                                                             <  2:379156/150‑1 (MQ=255)
                                                          ggATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCt                                                                                             >  1:1545080/1‑150 (MQ=255)
                                                               aaaTAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGtttt                                                                                        >  1:273478/1‑150 (MQ=255)
                                                                aaTAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTc                                                                                       <  2:1105467/150‑1 (MQ=255)
                                                                    gTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGGTGTTTTCACTc                                                                                   >  2:432870/1‑150 (MQ=255)
                                                                     ttcTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTTGTCATATCCTATATAGCCCCTGGGGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCg                                                                                  <  2:1586032/148‑1 (MQ=255)
                                                                     tGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCg                                                                                  >  1:1675116/1‑150 (MQ=255)
                                                                        tgtttACTAAGAGTTCTACTCCAGGTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGGCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCgttg                                                                               <  2:775083/148‑1 (MQ=255)
                                                                         ttttACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCgttgt                                                                              >  1:1424656/1‑150 (MQ=255)
                                                                           ttACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACTGTATTAGGCATATCCTATATAGCCCATGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGttgttg                                                                            <  1:272863/150‑1 (MQ=255)
                                                                           ttACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGttgttg                                                                            <  1:628176/150‑1 (MQ=255)
                                                                            tACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGc                                                                           >  1:543952/1‑150 (MQ=255)
                                                                              cTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCa                                                                          >  1:253128/1‑149 (MQ=255)
                                                                               tAAGAGTTCTACTCCATTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATc                                                                        <  1:1444828/150‑1 (MQ=255)
                                                                                aaGAGTTCTACTCCAGTTCCGGACTGATCACGCAACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCTCCAGGTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTGCAATAAGCTGTTTTCCCTCATTGTTGGATCt                                                                       >  2:254483/1‑150 (MQ=255)
                                                                                       cTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTgg                                                                >  1:1121450/1‑150 (MQ=255)
                                                                                        tACTCCAGTTCCGGACTGCTCCCGCCACGGTATTAGGCATATCCTATATAGCCCCTGGGGAGAGTCACCAGTTCCTGGATTAAATAAAATGGAGTTTTACATGTAGGCTTTCAATAAGGTGTTTTCCGTCGTTGTTGCATctggtctggt                                                               >  2:90613/1‑150 (MQ=255)
                                                                                        tACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGt                                                               >  1:490181/1‑150 (MQ=255)
                                                                                         aCTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGtt                                                              >  2:247548/1‑150 (MQ=255)
                                                                                         aCTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGtt                                                              >  2:369173/1‑150 (MQ=255)
                                                                                           tCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGGGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTATCCCTCGTTGTTGCATCTGTTCTGGtttt                                                            <  1:1141156/150‑1 (MQ=255)
                                                                                            ccAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATTTAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTtc                                                           <  1:487361/150‑1 (MQ=255)
                                                                                            ccAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTtc                                                           >  2:1340518/1‑150 (MQ=255)
                                                                                            ccAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTtc                                                           >  2:589647/1‑150 (MQ=255)
                                                                                             cAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTtct                                                          <  2:1331799/150‑1 (MQ=255)
                                                                                             cAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTtct                                                          <  1:1391053/150‑1 (MQ=255)
                                                                                              aGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTtctc                                                         <  1:56624/150‑1 (MQ=255)
                                                                                              aGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTtctc                                                         <  1:451253/150‑1 (MQ=255)
                                                                                               gTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCGCTCGTTGTTGCATCTGTTCTGGTTTtctct                                                        >  1:674095/1‑150 (MQ=255)
                                                                                                    ggACTGCTCACGCCACGGTATAAGGCATATCCTAGATAGCCCCTGCGGAGCGTCACCAGTTCCTGGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCACTAAGCTGTTATCCCTCGATGTTGCATCTGGTCGGGTTTTCTCTCTTgc                                                   >  2:665906/1‑150 (MQ=255)
                                                                                                       cTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTgctgg                                                >  1:188817/1‑150 (MQ=255)
                                                                                                            cACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCACTCGTTGTTGCATCTGTTCTGGTTTTCTCTCATGCTGGCTGCg                                           >  1:959660/1‑150 (MQ=255)
                                                                                                             aCGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCgg                                          <  1:927414/150‑1 (MQ=255)
                                                                                                             aCGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCgg                                          <  1:1190473/150‑1 (MQ=255)
                                                                                                              cGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGt                                         <  2:41625/150‑1 (MQ=255)
                                                                                                              cGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGAATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGt                                         >  1:1084448/1‑150 (MQ=255)
                                                                                                               gCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTg                                        <  1:1263791/150‑1 (MQ=255)
                                                                                                                ccACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCCCCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGa                                       >  1:43174/1‑150 (MQ=255)
                                                                                                                     gTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAaag                                  <  2:1727984/150‑1 (MQ=255)
                                                                                                                       aTTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATTAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAaagaa                                <  1:1581160/150‑1 (MQ=255)
                                                                                                                         tAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAagaaga                              <  1:929607/150‑1 (MQ=255)
                                                                                                                          aGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAgaagaa                             >  1:1490028/1‑150 (MQ=255)
                                                                                                                             cATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCGCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGATTCg                          >  1:473619/1‑150 (MQ=255)
                                                                                                                             cATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCg                          <  2:166806/150‑1 (MQ=255)
                                                                                                                                atCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCgaag                       <  2:273478/150‑1 (MQ=255)
                                                                                                                                 tCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTATTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCgaaga                      <  2:424257/150‑1 (MQ=255)
                                                                                                                                   cTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCGAAGaaa                    <  2:687439/150‑1 (MQ=255)
                                                                                                                                         aggaacTGGTGACTCTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCGAAGAAATTCAgc              >  2:1481034/6‑150 (MQ=255)
                                                                                                                                         aGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCGAAGAAATTCAgc              <  2:616152/150‑1 (MQ=255)
                                                                                                                                          ggaacTGGTGACTCTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGATTCGAAGAAATTCAgcg             >  1:386108/5‑150 (MQ=255)
                                                                                                                                           ccccTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCGAAGAAATTCAgcgc            >  2:1493477/1‑150 (MQ=255)
                                                                                                                                             ccTGGTGAGAGTCCCCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCAGCTGTTCTGGTTTTCTCTATTGCTGGCTGCGGTGACAAAGAAGAATCGAAGAAATTCAGCGCCa          >  1:1505328/1‑150 (MQ=255)
                                                                                                                                              cTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCGAAGAAATTCAGCGCCaa         >  2:1262439/1‑150 (MQ=255)
                                                                                                                                                ggTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCGAAGAAATTCAGCGCCAATc       >  2:630352/1‑150 (MQ=255)
                                                                                                                                                ggTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCGAAGAAATTCAGCGCCAATc       <  2:1121450/150‑1 (MQ=255)
                                                                                                                                                 gTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCGAAGAAATTCAGCGCCAATCt      >  1:1094835/1‑150 (MQ=255)
                                                                                                                                                     gagTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCGAAGAAATTCAGCGCCAATCTGAAc  <  1:1338790/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCGAAGAAATTCAGCGCCAATCTGAAC  >  NC_000913/2211037‑2211335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑