Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,211,187 A→T 7.0% intergenic (+87/‑38) yehQ → / → yehR pseudogene/lipoprotein, DUF1307 family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,211,1870AT 7.0% 294.2 / 9.3 105intergenic (+87/‑38)yehQ/yehRpseudogene/lipoprotein, DUF1307 family
Reads supporting (aligned to +/- strand):  ref base A (54/42);  new base T (2/5);  total (58/47)
Fisher's exact test for biased strand distribution p-value = 2.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.04e-01

Only 100 of 109 total aligned reads displayed.

GCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCGAAGAAATTCAGCGCCAATCTGAAC  >  NC_000913/2211038‑2211335
                                                                                                                                                     |                                                                                                                                                    
gCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGAATTAGGCATATCCTATATAGCCCCTGGTgaga                                                                                                                                                      >  2:929607/1‑150 (MQ=255)
 cAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGTACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGagag                                                                                                                                                     >  2:1263051/1‑150 (MQ=255)
    tGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCa                                                                                                                                                  >  2:927414/1‑150 (MQ=255)
     gAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCCCGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTccc                                                                                                                                                 >  2:534283/1‑148 (MQ=255)
     gAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCAc                                                                                                                                                 >  1:408256/1‑150 (MQ=255)
      aaTAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCAcc                                                                                                                                                <  2:1291154/150‑1 (MQ=255)
      aaTAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCAcc                                                                                                                                                <  1:678686/150‑1 (MQ=255)
       aTAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCa                                                                                                                                               <  1:271883/150‑1 (MQ=255)
         aaCGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCCGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTGCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATTTCCTATATCGCACCTGGTGAGAGTCACGAGt                                                                                                                                             >  2:1501832/1‑150 (MQ=255)
          aCGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGACTCTCACCAggg                                                                                                                                            <  2:1180535/150‑3 (MQ=255)
          aCGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGACTCTCACCAggg                                                                                                                                            <  2:411571/150‑3 (MQ=255)
          aCGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGACTCTCACCAggg                                                                                                                                            <  1:215324/150‑3 (MQ=255)
            ggTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGACTCTCACCAggggc                                                                                                                                          <  2:1723039/150‑5 (MQ=255)
               gtTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTg                                                                                                                                       <  2:1579213/150‑1 (MQ=255)
               gtTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTg                                                                                                                                       >  1:1092798/1‑150 (MQ=255)
                    gctttgcGAACAATTATTCCCCATGCTTCAGCAACAGGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATtaa                                                                                                                                  >  1:1695425/1‑150 (MQ=255)
                    gctttgcGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGAGCTTTTTACTAAGAGTTCTACTCAAGTTACGGCCTGCTCACGCCACGGTATTAGGCATATGCGTTATAGCCCCTGGTGTGGGTGACCAGTTCCTTGATtaa                                                                                                                                  >  2:112039/1‑150 (MQ=255)
                       ttgcGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATtaaata                                                                                                                               >  1:774183/1‑150 (MQ=255)
                           gAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAAt                                                                                                                           >  1:1506178/1‑150 (MQ=255)
                            aaCAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATg                                                                                                                          >  1:1555721/1‑150 (MQ=255)
                              cAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCGCGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGa                                                                                                                        <  2:1173400/150‑1 (MQ=255)
                              cAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGa                                                                                                                        >  1:1722177/1‑150 (MQ=255)
                              cAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGAGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACAGCTCACGCCACGGTATTAGTCATATCCTATATAGCCCCTGGTGAGAGACACAACTTCCTAGATTAAATAAAATGGa                                                                                                                        >  1:250803/1‑150 (MQ=255)
                               aaTTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAg                                                                                                                       >  2:173298/1‑150 (MQ=255)
                                   attCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGtttt                                                                                                                   >  2:559802/1‑150 (MQ=255)
                                     tCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTAc                                                                                                                 <  1:371858/150‑1 (MQ=255)
                                      ccccATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACa                                                                                                                >  2:1677054/1‑150 (MQ=255)
                                      ccccATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACa                                                                                                                <  1:1216811/150‑1 (MQ=255)
                                       cccATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACAt                                                                                                               >  1:436066/1‑150 (MQ=255)
                                        ccATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATg                                                                                                              >  1:646127/1‑150 (MQ=255)
                                        ccATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCAACGGTATTAGGCATATCCTATATAGCCACTGGTGAGAGTCAACAGTTCCTTGATAAAATAAAATGGAGTTTTACATg                                                                                                              >  1:1111783/1‑150 (MQ=255)
                                        ccATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTACAGTTACGGACTGATCACGCCACGGTATTAGGCATATCATATATAGCCCCTGGTGAGAGTCACAAGTTACTTGATTAAATAAAATGGAGTTTTAAAtt                                                                                                              >  1:1437444/1‑149 (MQ=255)
                                             cTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGc                                                                                                         >  1:953314/1‑150 (MQ=255)
                                             cTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGGGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGc                                                                                                         <  2:1271547/150‑1 (MQ=255)
                                              ttCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGCCTGCTGACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCt                                                                                                        >  2:299008/1‑150 (MQ=255)
                                                cAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCttt                                                                                                      >  1:41625/1‑150 (MQ=255)
                                                 aGCAACAAGGATGAAATAGTGCTTTTTACTAATTGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTc                                                                                                     <  1:1701323/150‑1 (MQ=255)
                                                    aacaaGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATGCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTTAATAAAATGGAGTTTTACATGAAGGCTTTCAAt                                                                                                  >  2:105363/1‑150 (MQ=255)
                                                    aacaaGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGACACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAAt                                                                                                  >  2:1338790/1‑150 (MQ=255)
                                                       aaGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAGATGGAGTTTTACATGAAGGCTTTCAATAAg                                                                                               <  1:1025714/150‑1 (MQ=255)
                                                       aaGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAg                                                                                               <  1:1492356/150‑1 (MQ=255)
                                                       aaGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAg                                                                                               <  2:1496424/150‑1 (MQ=255)
                                                        aGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTATGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATAGAGTTTTACATGAAGGCTTTCAATAAGc                                                                                              <  1:733230/150‑1 (MQ=255)
                                                        aGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGc                                                                                              <  2:1550761/150‑1 (MQ=255)
                                                         ggATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCt                                                                                             <  2:379156/150‑1 (MQ=255)
                                                         ggATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCt                                                                                             >  1:1545080/1‑150 (MQ=255)
                                                              aaaTAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGtttt                                                                                        >  1:273478/1‑150 (MQ=255)
                                                                   gTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGGTGTTTTCACTc                                                                                   >  2:432870/1‑150 (MQ=255)
                                                                    ttcTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTTGTCATATCCTATATAGCCCCTGGGGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCg                                                                                  <  2:1586032/148‑1 (MQ=255)
                                                                    tGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCg                                                                                  >  1:1675116/1‑150 (MQ=255)
                                                                       tgtttACTAAGAGTTCTACTCCAGGTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGGCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCgttg                                                                               <  2:775083/148‑1 (MQ=255)
                                                                        ttttACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCgttgt                                                                              >  1:1424656/1‑150 (MQ=255)
                                                                          ttACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACTGTATTAGGCATATCCTATATAGCCCATGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGttgttg                                                                            <  1:272863/150‑1 (MQ=255)
                                                                           tACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGc                                                                           >  1:543952/1‑150 (MQ=255)
                                                                             cTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCa                                                                          >  1:253128/1‑149 (MQ=255)
                                                                             cTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCa                                                                          <  2:253128/149‑1 (MQ=255)
                                                                              tAAGAGTTCTACTCCATTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATc                                                                        <  1:1444828/150‑1 (MQ=255)
                                                                               aaGAGTTCTACTCCAGTTCCGGACTGATCACGCAACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCTCCAGGTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTGCAATAAGCTGTTTTCCCTCATTGTTGGATCt                                                                       >  2:254483/1‑150 (MQ=255)
                                                                                      cTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTgg                                                                >  1:1121450/1‑150 (MQ=255)
                                                                                       tACTCCAGTTCCGGACTGCTCCCGCCACGGTATTAGGCATATCCTATATAGCCCCTGGGGAGAGTCACCAGTTCCTGGATTAAATAAAATGGAGTTTTACATGTAGGCTTTCAATAAGGTGTTTTCCGTCGTTGTTGCATctggtctggt                                                               >  2:90613/1‑150 (MQ=255)
                                                                                       tACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGt                                                               >  1:490181/1‑150 (MQ=255)
                                                                                        aCTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGtt                                                              >  2:369173/1‑150 (MQ=255)
                                                                                        aCTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGtt                                                              >  2:247548/1‑150 (MQ=255)
                                                                                          tCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGGGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTATCCCTCGTTGTTGCATCTGTTCTGGtttt                                                            <  1:1141156/150‑1 (MQ=255)
                                                                                           ccAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATTTAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTtc                                                           <  1:487361/150‑1 (MQ=255)
                                                                                           ccAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTtc                                                           >  2:1340518/1‑150 (MQ=255)
                                                                                           ccAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTtc                                                           >  2:589647/1‑150 (MQ=255)
                                                                                            cAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTtct                                                          <  2:1331799/150‑1 (MQ=255)
                                                                                             aGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTtctc                                                         <  1:451253/150‑1 (MQ=255)
                                                                                             aGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTtctc                                                         <  1:56624/150‑1 (MQ=255)
                                                                                              gTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCGCTCGTTGTTGCATCTGTTCTGGTTTtctct                                                        >  1:674095/1‑150 (MQ=255)
                                                                                                   ggACTGCTCACGCCACGGTATAAGGCATATCCTAGATAGCCCCTGCGGAGCGTCACCAGTTCCTGGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCACTAAGCTGTTATCCCTCGATGTTGCATCTGGTCGGGTTTTCTCTCTTgc                                                   >  2:665906/1‑150 (MQ=255)
                                                                                                      cTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTgctgg                                                >  1:188817/1‑150 (MQ=255)
                                                                                                           cACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCACTCGTTGTTGCATCTGTTCTGGTTTTCTCTCATGCTGGCTGCg                                           >  1:959660/1‑150 (MQ=255)
                                                                                                            aCGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCgg                                          <  1:1190473/150‑1 (MQ=255)
                                                                                                            aCGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCgg                                          <  1:927414/150‑1 (MQ=255)
                                                                                                             cGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGt                                         <  2:41625/150‑1 (MQ=255)
                                                                                                             cGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGAATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGt                                         >  1:1084448/1‑150 (MQ=255)
                                                                                                              gCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTg                                        <  1:1263791/150‑1 (MQ=255)
                                                                                                               ccACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCCCCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGa                                       >  1:43174/1‑150 (MQ=255)
                                                                                                                    gTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAaag                                  <  2:1727984/150‑1 (MQ=255)
                                                                                                                      aTTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATTAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAaagaa                                <  1:1581160/150‑1 (MQ=255)
                                                                                                                        tAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAagaaga                              <  1:929607/150‑1 (MQ=255)
                                                                                                                         aGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAgaagaa                             >  1:1490028/1‑150 (MQ=255)
                                                                                                                            cATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCGCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGATTCg                          >  1:473619/1‑150 (MQ=255)
                                                                                                                            cATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCg                          <  2:166806/150‑1 (MQ=255)
                                                                                                                               atCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCgaag                       >  1:1338149/1‑150 (MQ=255)
                                                                                                                               atCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCgaag                       <  2:273478/150‑1 (MQ=255)
                                                                                                                                tCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTATTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCgaaga                      <  2:424257/150‑1 (MQ=255)
                                                                                                                                  cTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCGAAGaaa                    <  2:687439/150‑1 (MQ=255)
                                                                                                                                        aGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCGAAGAAATTCAgc              >  1:739173/1‑150 (MQ=255)
                                                                                                                                        aGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCGAAGAAATTCAgc              <  2:616152/150‑1 (MQ=255)
                                                                                                                                          ccccTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCGAAGAAATTCAgcgc            >  2:1493477/1‑150 (MQ=255)
                                                                                                                                            ccTGGTGAGAGTCCCCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCAGCTGTTCTGGTTTTCTCTATTGCTGGCTGCGGTGACAAAGAAGAATCGAAGAAATTCAGCGCCa          >  1:1505328/1‑150 (MQ=255)
                                                                                                                                             cTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCGAAGAAATTCAGCGCCaa         >  2:1262439/1‑150 (MQ=255)
                                                                                                                                               ggTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCGAAGAAATTCAGCGCCAATc       <  2:1121450/150‑1 (MQ=255)
                                                                                                                                               ggTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCGAAGAAATTCAGCGCCAATc       >  2:630352/1‑150 (MQ=255)
                                                                                                                                                gTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCGAAGAAATTCAGCGCCAATCt      >  1:1094835/1‑150 (MQ=255)
                                                                                                                                                    gagTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCGAAGAAATTCAGCGCCAATCTGAAc  <  1:1338790/150‑1 (MQ=255)
                                                                                                                                                    gagTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCGAAGAAATTCAGCGCCAATCTGAAc  >  1:456018/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAGTTCTACTCCAGTTCCGGACTGCTCACGCCACGGTATTAGGCATATCCTATATAGCCCCTGGTGAGAGTCACCAGTTCCTTGATTAAATAAAATGGAGTTTTACATGAAGGCTTTCAATAAGCTGTTTTCCCTCGTTGTTGCATCTGTTCTGGTTTTCTCTCTTGCTGGCTGCGGTGACAAAGAAGAATCGAAGAAATTCAGCGCCAATCTGAAC  >  NC_000913/2211038‑2211335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑