Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,211,706 T→A 5.4% intergenic (+20/+20) yehR → / ← yehS lipoprotein, DUF1307 family/DUF1456 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,211,7060TA 5.4% 274.0 / 4.5 92intergenic (+20/+20)yehR/yehSlipoprotein, DUF1307 family/DUF1456 family protein
Reads supporting (aligned to +/- strand):  ref base T (48/39);  new base A (2/3);  total (50/42)
Fisher's exact test for biased strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.02e-01

AAAAGTGGATTTTAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAAC  >  NC_000913/2211557‑2211854
                                                                                                                                                     |                                                                                                                                                    
aaaaGTGGATTTTAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGctct                                                                                                                                                      <  2:127064/150‑1 (MQ=255)
aaaaGTGGATTTTAAAGCACTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCAATATGTCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGctct                                                                                                                                                      <  2:1021340/150‑1 (MQ=255)
    gTGGATTTTAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAAc                                                                                                                                                  >  1:1618067/1‑150 (MQ=255)
    gTGGATTTTAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAAc                                                                                                                                                  <  1:1232455/150‑1 (MQ=255)
    gTGGATTTTAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGGGAAATAATCGGTTGGCGGTCATGCTCTAAAc                                                                                                                                                  <  2:1125570/150‑1 (MQ=255)
     tGGATTTTAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACa                                                                                                                                                 <  1:648507/150‑1 (MQ=255)
       gATTTTAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATg                                                                                                                                               >  2:1344111/1‑150 (MQ=255)
        aTTTTAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGa                                                                                                                                              >  1:1539065/1‑150 (MQ=255)
          tttAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGAcc                                                                                                                                            >  1:690231/1‑150 (MQ=255)
           ttAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGTTTAGAGCATGACCg                                                                                                                                           <  1:35942/150‑1 (MQ=255)
           ttAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCg                                                                                                                                           <  2:1428102/150‑1 (MQ=255)
            tAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATGACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGc                                                                                                                                          >  2:415921/1‑150 (MQ=255)
            tAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGTTTAGAGCATGACCGc                                                                                                                                          <  1:47478/150‑1 (MQ=255)
             aaaGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGca                                                                                                                                         >  2:1368662/1‑149 (MQ=255)
             aaaGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTAGCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGcc                                                                                                                                         <  1:1252668/150‑1 (MQ=255)
              aaGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGTTTAGAGCATGACCGCCa                                                                                                                                        <  2:488223/150‑1 (MQ=255)
                       aGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAg                                                                                                                               <  1:1391540/150‑1 (MQ=255)
                       aGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAg                                                                                                                               >  2:752343/1‑150 (MQ=255)
                             tttCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCtttt                                                                                                                         >  1:1439666/1‑150 (MQ=255)
                              ttCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTTGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCttttt                                                                                                                        <  1:1636929/150‑1 (MQ=255)
                               tCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCtttttt                                                                                                                       <  2:1618067/150‑1 (MQ=255)
                                cAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTc                                                                                                                      >  1:435978/1‑150 (MQ=255)
                                cAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTc                                                                                                                      >  2:270492/1‑150 (MQ=255)
                                 aGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTca                                                                                                                     <  1:1460252/150‑1 (MQ=255)
                                  ggAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTcac                                                                                                                    >  1:202026/1‑150 (MQ=255)
                                  ggAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTcac                                                                                                                    >  2:633011/1‑150 (MQ=255)
                                   gAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTcaca                                                                                                                   >  1:248880/1‑150 (MQ=255)
                                     aTCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATg                                                                                                                 <  2:1245225/150‑1 (MQ=255)
                                      tCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAGGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGc                                                                                                                <  2:807198/150‑1 (MQ=255)
                                             ttCTGCTGAAGATGCCAAAAAAGGTATCACTATGGTGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGgcgcg                                                                                                         <  1:457016/150‑1 (MQ=255)
                                                tgctgAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTg                                                                                                      >  1:926934/1‑150 (MQ=255)
                                                 gctgAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGc                                                                                                     >  1:733128/1‑150 (MQ=255)
                                                  ctgAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGcc                                                                                                    <  1:1012100/150‑1 (MQ=255)
                                                    gAAGATGCCAAAAAATGTATCACTATGGCGCAAATGGAACTGGTGATGACAGCCGCTGGGTTTAAAGAAGGGAAATAATCGGCTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAg                                                                                                  <  1:980202/150‑1 (MQ=255)
                                                    gAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGTCCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAg                                                                                                  <  2:1599114/150‑1 (MQ=255)
                                                    gAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAg                                                                                                  >  2:1616117/1‑150 (MQ=255)
                                                    gAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAg                                                                                                  >  1:944801/1‑150 (MQ=255)
                                                      aGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTc                                                                                                >  2:546267/1‑150 (MQ=255)
                                                           cgaaaaaaGGTATCAGTATTGCTCAAATGGATCTGTTGATTAAAGACGATTCTTTTAAAGAAGTGAAATAATCTGTTGCCGGTCATGCTCTAAACATGACCGCCATTTTTTTAGCCTTTTTTAACATGCTGGCGCGCTGCCAGTCCAGGc                                                                                           <  1:314271/148‑1 (MQ=255)
                                                           ccAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGc                                                                                           >  2:361480/1‑150 (MQ=255)
                                                            cAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAAACGGTTGCCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCa                                                                                          <  1:362412/150‑1 (MQ=255)
                                                             aaaaaaGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCACCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAg                                                                                         <  2:1329788/150‑1 (MQ=255)
                                                              aaaaaGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGa                                                                                        <  1:1344111/150‑1 (MQ=255)
                                                                     tATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAAc                                                                                 >  2:1304303/1‑150 (MQ=255)
                                                                      aTCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACg                                                                                >  2:1439143/1‑150 (MQ=255)
                                                                        cACTATGGCGCAAATGGATCTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGTAATAATCGGATGGGGGTCATGCTCTAAACATGAGGGCCAATTTTTTTGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCGACGCAGAAAATAACGTa                                                                              <  2:1455589/150‑1 (MQ=255)
                                                                         aCTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTaa                                                                             >  2:1208997/1‑150 (MQ=255)
                                                                             tGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAAt                                                                         <  2:1061882/150‑1 (MQ=255)
                                                                             tGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAAt                                                                         <  2:585023/150‑1 (MQ=255)
                                                                             tGGAGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAAt                                                                         <  1:1368662/150‑1 (MQ=255)
                                                                              ggCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAATAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCAAGTCCACGCAGAAAATAACGTAAAAAtt                                                                        <  2:369151/150‑1 (MQ=255)
                                                                               gcgcAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGATATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAATATTg                                                                       >  2:1500894/1‑150 (MQ=255)
                                                                                cgcAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGa                                                                      >  2:565819/1‑150 (MQ=255)
                                                                                  cacctGGAACTGGTGATGAAAGCCGCTGGTTTTAAACACGTGTCATAATCGGTTGGCGGGCATGCTCTAAACATGACCGACAATTTTTAAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCCGAAACTAACGTAAAAATTGATc                                                                    <  2:315791/146‑1 (MQ=255)
                                                                                   aaaTGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATcg                                                                   <  2:763469/150‑1 (MQ=255)
                                                                                    aaTGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATcgc                                                                  >  1:1472362/1‑150 (MQ=255)
                                                                                        gAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCa                                                              >  2:1688397/1‑150 (MQ=255)
                                                                                         aaCGGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCAt                                                             <  2:1725174/150‑1 (MQ=255)
                                                                                           cTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTc                                                           >  1:782077/1‑150 (MQ=255)
                                                                                                gatgaAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGa                                                      >  2:459032/1‑150 (MQ=255)
                                                                                                  tgaAAGCCGCTGGTTTTAAAGAAGAGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAg                                                    >  2:1556915/1‑150 (MQ=255)
                                                                                                   gaAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGt                                                   >  1:563140/1‑150 (MQ=255)
                                                                                                    aaaGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGtt                                                  >  1:118038/1‑150 (MQ=255)
                                                                                                     aaGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGttt                                                 <  1:1616117/150‑1 (MQ=255)
                                                                                                      aGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGtttt                                                >  1:125321/1‑150 (MQ=255)
                                                                                                       gCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGttttt                                               >  2:467591/1‑150 (MQ=255)
                                                                                                       gCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGttttt                                               >  1:742341/1‑150 (MQ=255)
                                                                                                           cTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGa                                           <  2:590081/150‑1 (MQ=255)
                                                                                                              gTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATcc                                        >  2:1724233/1‑150 (MQ=255)
                                                                                                               ttttAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCg                                       >  1:936026/1‑150 (MQ=255)
                                                                                                                  tAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTg                                    >  2:590368/1‑150 (MQ=255)
                                                                                                                  tAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTg                                    >  1:1331575/1‑150 (MQ=255)
                                                                                                                     agaagTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTgcgc                                 >  1:1039287/1‑150 (MQ=255)
                                                                                                                     agaagTGAAATAATCGGTTGGCGGTCATGCGCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTgcgc                                 <  1:1221784/150‑1 (MQ=255)
                                                                                                                      gaagTGAAATAATCGGTTGGCGGGCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTgcgcg                                <  1:1344506/150‑1 (MQ=255)
                                                                                                                       aagTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTTGCCTGTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAATTAACGTAAAAATTGATCGCCGCATACGCGGATGTTTTTATGATCCGGTgcgcgc                               >  2:1104506/1‑150 (MQ=255)
                                                                                                                         gTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGcat                             <  2:1591091/150‑1 (MQ=255)
                                                                                                                          tGAAATAATCGGTTGGCGGTCACGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGcatc                            >  1:1097536/1‑150 (MQ=255)
                                                                                                                             aataatCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCatcatc                         <  1:752343/150‑1 (MQ=255)
                                                                                                                               taatCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGc                       <  1:565819/150‑1 (MQ=255)
                                                                                                                                aatCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCt                      <  1:775479/150‑1 (MQ=255)
                                                                                                                                 atCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTg                     <  2:1439666/150‑1 (MQ=255)
                                                                                                                                  tCGGTTGGCGGTCATGCTCTAAACATGCCCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATACGGTGCGCGCATCAGCGCTGt                    >  2:1390340/1‑150 (MQ=255)
                                                                                                                                  tCGGATGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTATTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGt                    <  1:268563/150‑1 (MQ=255)
                                                                                                                                    ggTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTaa                  <  2:248880/150‑1 (MQ=255)
                                                                                                                                     attGGCGGTCATGGTTAGAGCATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAAt                 >  1:1053372/2‑150 (MQ=255)
                                                                                                                                      ttGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCCCGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAAtt                >  2:1375931/1‑150 (MQ=255)
                                                                                                                                        ggCGGTCATGTTTAGAGCATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTc              >  2:641641/1‑150 (MQ=255)
                                                                                                                                        ggCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTc              >  1:738513/1‑150 (MQ=255)
                                                                                                                                            gTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGc          >  2:1580667/1‑150 (MQ=255)
                                                                                                                                            gTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGc          >  1:125831/1‑150 (MQ=255)
                                                                                                                                            gTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGc          <  2:690231/150‑1 (MQ=255)
                                                                                                                                                tGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCg      >  2:1668433/1‑150 (MQ=255)
                                                                                                                                                 gCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGa     >  1:1333682/1‑150 (MQ=255)
                                                                                                                                                  ctctAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTGTTATGTGCCGGTGCGCGCCTCAGCGCTGTACTTTCCGGCATCGac    >  1:331956/1‑149 (MQ=255)
                                                                                                                                                  ctctAAACATGACCGACAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCAACGCAGAAAATAACGTAAAAATTGATCTCCGAATTCGCGGAAGTTTTTATGATCCGGTGAGCGCATAATCTCTGTAATTTCAGTCATCTaa    >  1:1014688/1‑150 (MQ=255)
                                                                                                                                                    ctAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAAc  >  2:1285258/1‑150 (MQ=255)
                                                                                                                                                    ctAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAAc  >  1:1541693/1‑150 (MQ=255)
                                                                                                                                                    ctAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGATTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAAc  >  1:106667/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AAAAGTGGATTTTAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAAC  >  NC_000913/2211557‑2211854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: