Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,211,707 A→G 5.1% intergenic (+21/+19) yehR → / ← yehS lipoprotein, DUF1307 family/DUF1456 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,211,7070AG 5.1% 298.6 / 4.8 97intergenic (+21/+19)yehR/yehSlipoprotein, DUF1307 family/DUF1456 family protein
Reads supporting (aligned to +/- strand):  ref base A (53/39);  new base G (2/3);  total (55/42)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.96e-01

GTGGATTTTAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAACGC  >  NC_000913/2211561‑2211856
                                                                                                                                                  |                                                                                                                                                     
gTGGATTTTAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAAc                                                                                                                                                    >  1:1618067/1‑150 (MQ=255)
gTGGATTTTAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAAc                                                                                                                                                    <  1:1232455/150‑1 (MQ=255)
gTGGATTTTAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGGGAAATAATCGGTTGGCGGTCATGCTCTAAAc                                                                                                                                                    <  2:1125570/150‑1 (MQ=255)
 tGGATTTTAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACa                                                                                                                                                   <  1:648507/150‑1 (MQ=255)
   gATTTTAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATg                                                                                                                                                 >  2:1344111/1‑150 (MQ=255)
    aTTTTAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGa                                                                                                                                                >  1:1539065/1‑150 (MQ=255)
      tttAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGAcc                                                                                                                                              >  1:690231/1‑150 (MQ=255)
       ttAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGTTTAGAGCATGACCg                                                                                                                                             <  1:35942/150‑1 (MQ=255)
       ttAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCg                                                                                                                                             <  2:1428102/150‑1 (MQ=255)
        tAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATGACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGc                                                                                                                                            >  2:415921/1‑150 (MQ=255)
        tAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGTTTAGAGCATGACCGc                                                                                                                                            <  1:47478/150‑1 (MQ=255)
         aaaGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGca                                                                                                                                           >  2:1368662/1‑149 (MQ=255)
         aaaGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTAGCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGcc                                                                                                                                           <  1:1252668/150‑1 (MQ=255)
          aaGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGTTTAGAGCATGACCGCCa                                                                                                                                          <  2:488223/150‑1 (MQ=255)
                   aGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAg                                                                                                                                 <  1:1391540/150‑1 (MQ=255)
                   aGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAg                                                                                                                                 >  2:752343/1‑150 (MQ=255)
                         tttCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCtttt                                                                                                                           >  1:1439666/1‑150 (MQ=255)
                          ttCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTTGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCttttt                                                                                                                          <  1:1636929/150‑1 (MQ=255)
                           tCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCtttttt                                                                                                                         <  2:1618067/150‑1 (MQ=255)
                            cAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTc                                                                                                                        >  2:270492/1‑150 (MQ=255)
                            cAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTc                                                                                                                        >  1:435978/1‑150 (MQ=255)
                             aGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTca                                                                                                                       <  1:1460252/150‑1 (MQ=255)
                              ggAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTcac                                                                                                                      >  1:202026/1‑150 (MQ=255)
                              ggAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTcac                                                                                                                      >  2:633011/1‑150 (MQ=255)
                               gAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTcaca                                                                                                                     >  1:248880/1‑150 (MQ=255)
                                 aTCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATg                                                                                                                   <  2:1245225/150‑1 (MQ=255)
                                  tCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAGGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGc                                                                                                                  <  2:807198/150‑1 (MQ=255)
                                         ttCTGCTGAAGATGCCAAAAAAGGTATCACTATGGTGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGgcgcg                                                                                                           <  1:457016/150‑1 (MQ=255)
                                            tgctgAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTg                                                                                                        >  1:926934/1‑150 (MQ=255)
                                             gctgAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGc                                                                                                       >  1:733128/1‑150 (MQ=255)
                                              ctgAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGcc                                                                                                      <  1:1012100/150‑1 (MQ=255)
                                                gAAGATGCCAAAAAATGTATCACTATGGCGCAAATGGAACTGGTGATGACAGCCGCTGGGTTTAAAGAAGGGAAATAATCGGCTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAg                                                                                                    <  1:980202/150‑1 (MQ=255)
                                                gAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGTCCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAg                                                                                                    <  2:1599114/150‑1 (MQ=255)
                                                gAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAg                                                                                                    >  2:1616117/1‑150 (MQ=255)
                                                gAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAg                                                                                                    >  1:944801/1‑150 (MQ=255)
                                                  aGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTc                                                                                                  >  2:546267/1‑150 (MQ=255)
                                                       cgaaaaaaGGTATCAGTATTGCTCAAATGGATCTGTTGATTAAAGACGATTCTTTTAAAGAAGTGAAATAATCTGTTGCCGGTCATGCTCTAAACATGACCGCCATTTTTTTAGCCTTTTTTAACATGCTGGCGCGCTGCCAGTCCAGGc                                                                                             <  1:314271/148‑1 (MQ=255)
                                                       ccAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGc                                                                                             >  2:361480/1‑150 (MQ=255)
                                                        cAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAAACGGTTGCCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCa                                                                                            <  1:362412/150‑1 (MQ=255)
                                                         aaaaaaGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCACCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAg                                                                                           <  2:1329788/150‑1 (MQ=255)
                                                          aaaaaGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGa                                                                                          <  1:1344111/150‑1 (MQ=255)
                                                                 tATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAAc                                                                                   >  2:1304303/1‑150 (MQ=255)
                                                                  aTCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACg                                                                                  >  2:1439143/1‑150 (MQ=255)
                                                                    cACTATGGCGCAAATGGATCTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGTAATAATCGGATGGGGGTCATGCTCTAAACATGAGGGCCAATTTTTTTGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCGACGCAGAAAATAACGTa                                                                                <  2:1455589/150‑1 (MQ=255)
                                                                     aCTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTaa                                                                               >  2:1208997/1‑150 (MQ=255)
                                                                         tGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAAt                                                                           <  2:1061882/150‑1 (MQ=255)
                                                                         tGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAAt                                                                           <  2:585023/150‑1 (MQ=255)
                                                                         tGGAGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAAt                                                                           <  1:1368662/150‑1 (MQ=255)
                                                                          ggCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAATAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCAAGTCCACGCAGAAAATAACGTAAAAAtt                                                                          <  2:369151/150‑1 (MQ=255)
                                                                           gcgcAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGATATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAATATTg                                                                         >  2:1500894/1‑150 (MQ=255)
                                                                            cgcAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGa                                                                        >  2:565819/1‑150 (MQ=255)
                                                                              cacctGGAACTGGTGATGAAAGCCGCTGGTTTTAAACACGTGTCATAATCGGTTGGCGGGCATGCTCTAAACATGACCGACAATTTTTAAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCCGAAACTAACGTAAAAATTGATc                                                                      <  2:315791/146‑1 (MQ=255)
                                                                               aaaTGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATcg                                                                     <  2:763469/150‑1 (MQ=255)
                                                                                aaTGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATcgc                                                                    >  1:1472362/1‑150 (MQ=255)
                                                                                    gAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCa                                                                >  2:1688397/1‑150 (MQ=255)
                                                                                     aaCGGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCAt                                                               <  2:1725174/150‑1 (MQ=255)
                                                                                       cTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTc                                                             >  1:782077/1‑150 (MQ=255)
                                                                                            gatgaAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGa                                                        >  2:459032/1‑150 (MQ=255)
                                                                                              tgaAAGCCGCTGGTTTTAAAGAAGAGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAg                                                      >  2:1556915/1‑150 (MQ=255)
                                                                                               gaAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGt                                                     >  1:563140/1‑150 (MQ=255)
                                                                                                aaaGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGtt                                                    >  1:118038/1‑150 (MQ=255)
                                                                                                 aaGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGttt                                                   <  1:1616117/150‑1 (MQ=255)
                                                                                                  aGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGtttt                                                  >  1:125321/1‑150 (MQ=255)
                                                                                                   gCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGttttt                                                 >  2:467591/1‑150 (MQ=255)
                                                                                                   gCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGttttt                                                 >  1:742341/1‑150 (MQ=255)
                                                                                                       cTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGa                                             <  2:590081/150‑1 (MQ=255)
                                                                                                          gTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATcc                                          >  2:1724233/1‑150 (MQ=255)
                                                                                                           ttttAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCg                                         >  1:936026/1‑150 (MQ=255)
                                                                                                              tAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTg                                      >  2:590368/1‑150 (MQ=255)
                                                                                                              tAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTg                                      >  1:1331575/1‑150 (MQ=255)
                                                                                                                 agaagTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTgcgc                                   >  1:1039287/1‑150 (MQ=255)
                                                                                                                 agaagTGAAATAATCGGTTGGCGGTCATGCGCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTgcgc                                   <  1:1221784/150‑1 (MQ=255)
                                                                                                                  gaagTGAAATAATCGGTTGGCGGGCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTgcgcg                                  <  1:1344506/150‑1 (MQ=255)
                                                                                                                   aagTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTTGCCTGTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAATTAACGTAAAAATTGATCGCCGCATACGCGGATGTTTTTATGATCCGGTgcgcgc                                 >  2:1104506/1‑150 (MQ=255)
                                                                                                                     gTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGcat                               <  2:1591091/150‑1 (MQ=255)
                                                                                                                      tGAAATAATCGGTTGGCGGTCACGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGcatc                              >  1:1097536/1‑150 (MQ=255)
                                                                                                                         aataatCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCatcatc                           <  1:752343/150‑1 (MQ=255)
                                                                                                                           taatCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGc                         <  1:565819/150‑1 (MQ=255)
                                                                                                                            aatCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCt                        <  1:775479/150‑1 (MQ=255)
                                                                                                                             atCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTg                       <  2:1439666/150‑1 (MQ=255)
                                                                                                                              tCGGTTGGCGGTCATGCTCTAAACATGCCCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATACGGTGCGCGCATCAGCGCTGt                      >  2:1390340/1‑150 (MQ=255)
                                                                                                                              tCGGATGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTATTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGt                      <  1:268563/150‑1 (MQ=255)
                                                                                                                                ggTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTaa                    <  2:248880/150‑1 (MQ=255)
                                                                                                                                 attGGCGGTCATGGTTAGAGCATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAAt                   >  1:1053372/2‑150 (MQ=255)
                                                                                                                                  ttGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCCCGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAAtt                  >  2:1375931/1‑150 (MQ=255)
                                                                                                                                    ggCGGTCATGTTTAGAGCATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTc                >  2:641641/1‑150 (MQ=255)
                                                                                                                                    ggCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTc                >  1:738513/1‑150 (MQ=255)
                                                                                                                                        gTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGc            >  2:1580667/1‑150 (MQ=255)
                                                                                                                                        gTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGc            >  1:125831/1‑150 (MQ=255)
                                                                                                                                        gTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGc            <  2:690231/150‑1 (MQ=255)
                                                                                                                                            tGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCg        >  2:1668433/1‑150 (MQ=255)
                                                                                                                                             gCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGa       >  1:1333682/1‑150 (MQ=255)
                                                                                                                                              ctctAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTGTTATGTGCCGGTGCGCGCCTCAGCGCTGTACTTTCCGGCATCGac      >  1:331956/1‑149 (MQ=255)
                                                                                                                                              ctctAAACATGACCGACAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCAACGCAGAAAATAACGTAAAAATTGATCTCCGAATTCGCGGAAGTTTTTATGATCCGGTGAGCGCATAATCTCTGTAATTTCAGTCATCTaa      >  1:1014688/1‑150 (MQ=255)
                                                                                                                                                ctAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAAc    >  2:1285258/1‑150 (MQ=255)
                                                                                                                                                ctAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAAc    >  1:1541693/1‑150 (MQ=255)
                                                                                                                                                ctAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGATTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAAc    >  1:106667/1‑150 (MQ=255)
                                                                                                                                                  aaaCATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAAcgc  <  2:742341/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GTGGATTTTAAAGCCCTGCAGGGTATTTCAGGAATCAACGTTTCTGCTGAAGATGCCAAAAAAGGTATCACTATGGCGCAAATGGAACTGGTGATGAAAGCCGCTGGTTTTAAAGAAGTGAAATAATCGGTTGGCGGTCATGCTCTAAACATGACCGCCAATTTTTTAGCCTTTTTTCACATGCTGGCGCGCTGCCAGTCCACGCAGAAAATAACGTAAAAATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAACGC  >  NC_000913/2211561‑2211856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: