Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,230,416 G→C 5.0% intergenic (‑31/‑208) dusC ← / → yohJ tRNA‑dihydrouridine synthase C/UPF0299 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,230,4160GC 5.0% 318.6 / 6.5 100intergenic (‑31/‑208)dusC/yohJtRNA‑dihydrouridine synthase C/UPF0299 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (55/40);  new base C (2/3);  total (57/43)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.75e-01

Only 100 of 102 total aligned reads displayed.

AGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATC  >  NC_000913/2230267‑2230565
                                                                                                                                                     |                                                                                                                                                     
agTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATg                                                                                                                                                       <  2:562203/150‑1 (MQ=255)
  ttGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGtt                                                                                                                                                     <  1:706691/150‑1 (MQ=255)
   tGATCCACAACGCGGACAAACTCGGTGATGCACAGATAGTAGTCGTTAAATTCGGTCAGCAATTCACGCAACAGAGAGTCAAGCACTCCCTCCATCGGTGCCTGTAACACACGCTTATCATCTCCCGCAAAAAAATGAGGCGGTATGTTa                                                                                                                                                    >  1:157720/1‑150 (MQ=255)
      tCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAgcg                                                                                                                                                 >  1:31992/1‑150 (MQ=255)
       ccaccaCGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAgcgc                                                                                                                                                >  2:1199761/1‑150 (MQ=255)
        caccacGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGcc                                                                                                                                               <  1:244387/150‑1 (MQ=255)
         accacGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCt                                                                                                                                              >  2:1492873/1‑150 (MQ=255)
          ccacGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTa                                                                                                                                             >  1:182280/1‑150 (MQ=255)
           cacGCTGACAAACTCGGTGATTCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGTTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTAt                                                                                                                                            <  2:157720/150‑1 (MQ=255)
           cacGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTAt                                                                                                                                            >  2:1661574/1‑150 (MQ=255)
           cacGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTAACATAGCGCCtca                                                                                                                                            <  1:561706/150‑3 (MQ=255)
            acGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATg                                                                                                                                           <  2:832293/150‑1 (MQ=255)
              gcgGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTAACATAGCGCCtcattt                                                                                                                                         <  2:1372997/150‑6 (MQ=255)
               cgGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACTCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTAACATAGCGCCtcatttt                                                                                                                                        <  1:1055183/150‑7 (MQ=255)
               cgGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGta                                                                                                                                        >  1:1133081/1‑149 (MQ=255)
                     aaCTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGa                                                                                                                                  >  1:444014/1‑150 (MQ=255)
                      aCTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGAt                                                                                                                                 <  2:470337/150‑1 (MQ=255)
                          ggTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTaaa                                                                                                                             >  1:792022/1‑150 (MQ=255)
                            tGATGCACAGATCGTAGTCGTTTACTTCGGTCAGCAATTCACGCACCAGTGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAgg                                                                                                                           <  2:194349/150‑1 (MQ=255)
                              aTGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTc                                                                                                                         <  2:1104758/150‑1 (MQ=255)
                                        cGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAgg                                                                                                               <  2:1406613/150‑1 (MQ=255)
                                         gTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAggg                                                                                                              >  1:1476346/1‑150 (MQ=255)
                                              cGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtgc                                                                                                         <  1:191211/150‑1 (MQ=255)
                                                   aCTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAATGTCTGCAAAGAGGGATTGCTGCaa                                                                                                    >  2:1551094/1‑150 (MQ=255)
                                                         gTCAGCAATTCACGCACCAGCGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATGAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCa                                                                                              >  1:78279/1‑150 (MQ=255)
                                                         gTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCATCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCaa                                                                                             <  2:49254/150‑1 (MQ=255)
                                                          tCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCaa                                                                                             <  2:1036065/150‑1 (MQ=255)
                                                           cAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGACTGCAAAGAGGGATTGCTGCAAACTGCAAt                                                                                            >  2:385972/1‑150 (MQ=255)
                                                            aGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGCGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATc                                                                                           >  2:291073/1‑150 (MQ=255)
                                                             gCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATcc                                                                                          >  2:443968/1‑150 (MQ=255)
                                                              cAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATccc                                                                                         <  2:1321541/150‑1 (MQ=255)
                                                              cAAATCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATccc                                                                                         <  2:722118/150‑1 (MQ=255)
                                                              aaaTTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACCCAAATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATccc                                                                                         <  2:406511/149‑1 (MQ=255)
                                                               aaTTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCct                                                                                        >  1:1158815/1‑150 (MQ=255)
                                                                aTTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTTACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCctc                                                                                       <  1:282269/150‑1 (MQ=255)
                                                                     cGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTAt                                                                                  <  1:1661574/150‑1 (MQ=255)
                                                                       cACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGc                                                                                >  2:121984/1‑150 (MQ=255)
                                                                       cACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGc                                                                                >  2:786520/1‑150 (MQ=255)
                                                                        aCCAGAGAGTCAAGCACTCCCTCCATCGGAGCCAGTACCACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAACGAGGGATTGCTGCACACTGCAATCCCTCTCTATGCt                                                                               >  1:528674/1‑150 (MQ=255)
                                                                          cAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTg                                                                             <  1:681698/150‑1 (MQ=255)
                                                                          cAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTg                                                                             >  2:665488/1‑150 (MQ=255)
                                                                          cAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTg                                                                             >  2:558395/1‑150 (MQ=255)
                                                                            gagagTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGAt                                                                           <  1:923743/150‑1 (MQ=255)
                                                                            gagagTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGAt                                                                           <  2:379152/150‑1 (MQ=255)
                                                                             agagTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATg                                                                          <  1:1392452/150‑1 (MQ=255)
                                                                                gTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTAt                                                                       >  1:1011525/1‑150 (MQ=255)
                                                                                gTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTAt                                                                       >  1:944279/1‑150 (MQ=255)
                                                                                  cAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGc                                                                     >  1:375999/1‑150 (MQ=255)
                                                                                  cAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCACCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGc                                                                     <  2:502283/150‑1 (MQ=255)
                                                                                   aaGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGcc                                                                    >  2:927548/1‑150 (MQ=255)
                                                                                   aaGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGcc                                                                    >  1:1460315/1‑150 (MQ=255)
                                                                                    aGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGTTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGcat                                                                   >  2:1721627/1‑148 (MQ=255)
                                                                                     gCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGTCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTg                                                                  <  2:691969/150‑1 (MQ=255)
                                                                                      cACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACACGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAATGGTCTGCAAAGAGGGATTGATTCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGa                                                                 >  1:837548/1‑150 (MQ=255)
                                                                                        ctccctccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGt                                                               <  2:1304669/150‑1 (MQ=255)
                                                                                        atccctccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCTCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGt                                                               <  2:1661993/149‑1 (MQ=255)
                                                                                           cctccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATAAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCg                                                            <  2:1026232/150‑1 (MQ=255)
                                                                                            ctccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGc                                                           >  2:780849/1‑150 (MQ=255)
                                                                                            atccATCGGTGCCAGTAACTCACGCATATCATCACCATCAAAAAAATGAGGCGCTATGTTAGCGCCTATTGTCAGCGGATTAATGGTATGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGc                                                           <  1:843948/149‑1 (MQ=255)
                                                                                              ccctCGGTGCCAGGAACACACGCATATCATCACCCGCAATCAAATGAGGCGCGATGTTAGCGCCTATGGTCAGCGGATTAAAGGGCGGCAAAGAGGGATTGCTGCAAACTGCAATCCCGCTCTATGCTTGATGTATGCCTGAGTTCGCTg                                                         <  1:886850/147‑1 (MQ=255)
                                                                                              ccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTg                                                         >  2:281801/1‑150 (MQ=255)
                                                                                              ccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTg                                                         >  2:1636153/1‑150 (MQ=255)
                                                                                              ccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTAGGGGCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTg                                                         <  2:844944/150‑1 (MQ=255)
                                                                                               cATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTgc                                                        <  2:182280/150‑1 (MQ=255)
                                                                                               cATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTgc                                                        <  1:152075/150‑1 (MQ=255)
                                                                                               cATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTgc                                                        <  1:978499/150‑1 (MQ=255)
                                                                                                aTCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTgcg                                                       >  2:264102/1‑150 (MQ=255)
                                                                                                aTCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTgcg                                                       <  1:1637140/150‑1 (MQ=255)
                                                                                                aTCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGGCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTgcg                                                       <  1:1630812/150‑1 (MQ=255)
                                                                                                 tCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGTTCAGCGGATTATAGGGCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTgcgc                                                      <  1:738675/150‑1 (MQ=255)
                                                                                                    gTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCAtt                                                   >  1:1366161/1‑150 (MQ=255)
                                                                                                     tGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAACGGGCTGCAAAGAGGGCTTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTg                                                  <  2:929225/150‑1 (MQ=255)
                                                                                                     tGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTg                                                  >  1:144569/1‑150 (MQ=255)
                                                                                                          gTACCACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTaa                                             <  2:1453491/150‑1 (MQ=255)
                                                                                                           tAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAAt                                            >  1:581832/1‑150 (MQ=255)
                                                                                                            aaCACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATg                                           >  2:1559174/1‑150 (MQ=255)
                                                                                                             acacacGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGc                                          >  1:704532/1‑150 (MQ=255)
                                                                                                             acacacGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGc                                          >  1:388248/1‑150 (MQ=255)
                                                                                                                    cATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAg                                   <  1:1426905/150‑1 (MQ=255)
                                                                                                                         catcaCCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGaaa                              >  2:878162/1‑150 (MQ=255)
                                                                                                                         catcaCCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGaaa                              >  2:1190873/1‑150 (MQ=255)
                                                                                                                             aCCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAAt                          >  1:272797/1‑150 (MQ=255)
                                                                                                                              cccGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATa                         >  1:1723016/1‑150 (MQ=255)
                                                                                                                                cGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAg                       >  2:1294248/1‑150 (MQ=255)
                                                                                                                                 gCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAgc                      >  1:1367/1‑150 (MQ=255)
                                                                                                                                    aaaaaaTGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTATCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCt                   >  1:584390/1‑150 (MQ=255)
                                                                                                                                          tGAGGCGCTATGTTCGCGCCTATGGTCAGCGGATTAAATGTCTGCAAAGAGGGATTGCTGCAAACTCCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCGGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTc             <  2:1715569/150‑1 (MQ=255)
                                                                                                                                           gAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCACACTGCAATCCCTCTCTATGCGTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCt            <  2:667869/150‑1 (MQ=255)
                                                                                                                                            aGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCtt           <  2:272797/150‑1 (MQ=255)
                                                                                                                                            aGGCGCTAACATAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCtt           >  2:452778/1‑150 (MQ=255)
                                                                                                                                             ggCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTa          >  1:1175689/1‑150 (MQ=255)
                                                                                                                                             ggCGCTAACATAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTa          >  1:1017982/1‑150 (MQ=255)
                                                                                                                                              gcgcTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTaa         >  1:1028046/1‑150 (MQ=255)
                                                                                                                                               cgcTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAAt        >  2:931081/1‑150 (MQ=255)
                                                                                                                                                 cTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAAtgt      >  2:630314/1‑150 (MQ=255)
                                                                                                                                                 cTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAAtgt      >  2:1365746/1‑150 (MQ=255)
                                                                                                                                                 cTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAAtgt      >  2:1027766/1‑150 (MQ=255)
                                                                                                                                                 cTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAAtgt      >  1:1288977/1‑150 (MQ=255)
                                                                                                                                                    tGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATTCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGAt   >  2:1198625/1‑150 (MQ=255)
                                                                                                                                                     gTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATc  >  2:1146138/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATC  >  NC_000913/2230267‑2230565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: