Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,242,884 T→G 10.1% L28L (CTA→CTC) ‡ yeiB ← DUF418 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,242,8840TG10.1% 307.8 / 18.8 115L28L (CTA→CTC) ‡yeiBDUF418 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (55/47);  new base G (6/6);  total (62/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

GGCAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTG  >  NC_000913/2242735‑2243031
                                                                                                                                                     |                                                                                                                                                   
ggCAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAATAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGt                                                                                                                                                     >  1:866073/1‑150 (MQ=255)
  cagcaACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGGACCCGGCGGGATTGAGATAAGCCGCCTTTGGTAg                                                                                                                                                   >  1:1093893/1‑150 (MQ=255)
     caACATTTGCACGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGccc                                                                                                                                                <  2:901023/150‑1 (MQ=255)
      aaCATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGAGTGAGATAAGCCGCCTTTGGTAGcccc                                                                                                                                               >  1:304801/1‑149 (MQ=255)
          tttGCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGCTGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAgg                                                                                                                                           <  1:954021/150‑1 (MQ=255)
          tttGCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAgg                                                                                                                                           <  2:1578876/150‑1 (MQ=255)
           ttGCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGgc                                                                                                                                          <  1:609810/150‑1 (MQ=255)
            tGCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCTAGTCCATGCATCCCGCGGCGTACTCGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGGCTACCAAAGgcg                                                                                                                                         <  2:1043494/150‑1 (MQ=255)
             gCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGgcgc                                                                                                                                        <  1:904816/150‑1 (MQ=255)
              cAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGGCTACCAAAGgcggc                                                                                                                                       <  1:589845/150‑3 (MQ=255)
              cAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGGCTACCAAAGgcggc                                                                                                                                       <  2:1644420/150‑3 (MQ=255)
              cAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCGCCTTTGGGCTACCAAAGgcggct                                                                                                                                      <  2:574593/150‑4 (MQ=255)
               aGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGACATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTg                                                                                                                                      <  1:1458142/150‑1 (MQ=255)
                ggCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGa                                                                                                                                     >  1:818174/1‑150 (MQ=255)
                 gCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGAt                                                                                                                                    <  2:1536790/150‑1 (MQ=255)
                  cccGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGGCTACCAAAGgcgg                                                                                                                                        >  1:736786/1‑144 (MQ=255)
                  cccGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGGCTACCAAAGgcgg                                                                                                                                        <  2:736786/145‑2 (MQ=255)
                    cGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGtt                                                                                                                                 <  2:4146/150‑1 (MQ=255)
                     gCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGttt                                                                                                                                <  1:1202496/150‑1 (MQ=255)
                     gCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCAACCCGCGGCTTAATAGCACCGTACCAGGAGGGATTGAGATAAGCCGCCTTTGCTAGCCCAAAGGCGCTGATGttt                                                                                                                                >  1:205764/1‑150 (MQ=255)
                     gCAACAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGttt                                                                                                                                <  2:1083419/150‑1 (MQ=255)
                      cACCAAACAGTAGCGCAAAACGCGGGAGGAATTTCCCCTGGCCGAGGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTa                                                                                                                               >  1:887240/1‑150 (MQ=255)
                      cACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTa                                                                                                                               <  2:1679515/150‑1 (MQ=255)
                        ccAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCAGGCCGATGAGAACAACAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAAt                                                                                                                             >  1:196549/1‑150 (MQ=255)
                          aaaCAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAG                                                                                                                           <  2:1344549‑M1/150‑1 (MQ=255)
                          aaaCAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAG                                                                                                                           >  1:857008‑M1/1‑150 (MQ=255)
                          aaaCAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCAGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCCCCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAG                                                                                                                           >  1:483393‑M1/1‑150 (MQ=255)
                              aGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGG                                                                                                                       >  1:241246‑M1/1‑150 (MQ=255)
                                  gcgcAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATcc                                                                                                                   >  2:1118666/1‑150 (MQ=255)
                                  gcgcAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATcc                                                                                                                   >  1:1172710/1‑150 (MQ=255)
                                     cAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCa                                                                                                                <  2:88368/150‑1 (MQ=255)
                                            cGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGc                                                                                                         <  2:815359/150‑1 (MQ=255)
                                             gTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCg                                                                                                        >  1:1482968/1‑150 (MQ=255)
                                               gAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGAc                                                                                                      >  1:1205076/1‑150 (MQ=255)
                                               gAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGAc                                                                                                      >  2:708244/1‑150 (MQ=255)
                                                 ggAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGAcgc                                                                                                    >  2:383895/1‑150 (MQ=255)
                                                    aTTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGGACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGAcgccgc                                                                                                 <  1:248570/150‑1 (MQ=255)
                                                     tttCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCcgcg                                                                                                <  2:731258/150‑1 (MQ=255)
                                                      ttCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGa                                                                                               >  1:421071/1‑150 (MQ=255)
                                                        cACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAAc                                                                                             <  2:1511963/150‑1 (MQ=255)
                                                        cACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAAc                                                                                             >  2:1547240/1‑150 (MQ=255)
                                                             ggCCGATGAGATCAAGAAATGCCCAAGTCCATGCTTCCGGGGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCCGTTTCCCCAGAATGGCGACGCCGCGAACAAAAt                                                                                        <  1:268143/150‑1 (MQ=255)
                                                                cGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGAaccatggagcgcaacgtcacgctcgattttgtt                                                                                     >  1:1524258‑M1/1‑117 (MQ=255)
                                                                 gATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGAAGCCCAAAGGCGCTGATGTTTATTAGCAGGATCCCCAGAATGGCGACGCCGCGCACAAAATCGAg                                                                                    >  2:305941/1‑150 (MQ=255)
                                                                  aTGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGc                                                                                   <  1:643188/150‑1 (MQ=255)
                                                                   tGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCGGATGTTTAATAGCAGGCTCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCg                                                                                  >  1:1486755/1‑150 (MQ=255)
                                                                    gagaTCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGt                                                                                 >  2:1034026/1‑150 (MQ=255)
                                                                     agaTCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTg                                                                                >  1:991323/1‑150 (MQ=255)
                                                                     agaTCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTg                                                                                >  2:636387/1‑150 (MQ=255)
                                                                           aGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTg                                                                          <  2:951288/150‑1 (MQ=255)
                                                                           aGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTg                                                                          <  1:551695/150‑1 (MQ=255)
                                                                              aaTGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTgcgc                                                                       >  2:712082/1‑150 (MQ=255)
                                                                                   ccAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCAt                                                                  >  1:651238/1‑150 (MQ=255)
                                                                                   ccAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCAt                                                                  <  1:1226600/150‑1 (MQ=255)
                                                                                     aaGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATgg                                                                <  2:421071/150‑1 (MQ=255)
                                                                                      aGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGCGCGTGACGTTGCGCTCCATGGt                                                               <  1:708244/150‑1 (MQ=255)
                                                                                       gTCCATGCATCGCTCGGCGTAATAGCACCGTACCAGGCGGGATTGAGTTAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGTGCGTGACGTTGCGCTCCATGGTt                                                              >  1:1071477‑M2/1‑149 (MQ=255)
                                                                                        tCCATGCATCCCGCGGCTTAATAGCACCGTACCAGGCTGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGTCGCCGCGAACAATATCGAGCGTGACGTTGCGCTCCATGGTtc                                                             >  1:1568963‑M2/1‑148 (MQ=255)
                                                                                         ccATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGAaccatggagcgcaacgtcacgctcgattttgttcgcggcgtcgccattctggggatcc                                                            <  2:1524258‑M1/150‑59 (MQ=255)
                                                                                         ccATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGACCAAAATCGAGCGTGACGTTGCGCTCCATGGTtcc                                                            <  1:1675876‑M2/150‑4 (MQ=255)
                                                                                         ccATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTtcc                                                            >  1:56748‑M2/1‑147 (MQ=255)
                                                                                          cATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGAGTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGAGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTtcct                                                           <  2:73446‑M2/150‑5 (MQ=255)
                                                                                             gCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGcc                                                        <  2:929613/150‑1 (MQ=255)
                                                                                             gCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGcc                                                        <  1:34347/150‑1 (MQ=255)
                                                                                              cATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCt                                                       >  2:1598419/1‑150 (MQ=255)
                                                                                                tCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCttt                                                     <  2:1671482/150‑1 (MQ=255)
                                                                                                tCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCttt                                                     <  1:827976/150‑1 (MQ=255)
                                                                                                gcccGCGGCTTAAGAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCGTTGGGAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGCAGGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGGTCCTGCCttt                                                     <  1:1060353/149‑1 (MQ=255)
                                                                                                  ccGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTa                                                   >  1:263941/1‑150 (MQ=255)
                                                                                                  ccGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTa                                                   <  2:1172710/150‑1 (MQ=255)
                                                                                                   cgcgGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGAaccatggagcgcaacgtcacgctcgattttgttcgcggcgtcgccattctggggatcctgctattaaa                                                  <  1:1546632‑M1/150‑69 (MQ=255)
                                                                                                       gcgTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAg                                              >  1:1700537/1‑150 (MQ=255)
                                                                                                        cgTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGt                                             <  2:1562430/150‑1 (MQ=255)
                                                                                                         gTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACTAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGtt                                            >  1:106720/1‑150 (MQ=255)
                                                                                                               ccACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATTTTTAATAGCAGGTTCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGGGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGtgatg                                      <  2:345875/149‑1 (MQ=255)
                                                                                                                cACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGtgatga                                     >  2:1397329/1‑150 (MQ=255)
                                                                                                                cACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGtgatga                                     >  2:91780/1‑150 (MQ=255)
                                                                                                                 aCCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGAc                                    <  1:212593/150‑1 (MQ=255)
                                                                                                                  ccGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGAAGTTTAAAAGCAGGATCACCAGAATGGAGACGCCGCGAACAAAATAGAGCGTGTCGTTGCGCTCCATGGTTCATGCCGTTTAATCAGTTGTGATGACg                                   >  1:629633/1‑150 (MQ=255)
                                                                                                                   cGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGc                                  <  2:857008/150‑1 (MQ=255)
                                                                                                                    gTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATAAGTTGTGATGACGca                                 >  1:1286349/1‑150 (MQ=255)
                                                                                                                     tACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGcac                                >  1:738865/1‑150 (MQ=255)
                                                                                                                         aGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAgcg                            >  1:1505234/1‑150 (MQ=255)
                                                                                                                           gCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAgcgcg                          <  1:1547240/150‑1 (MQ=255)
                                                                                                                            cGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAgcgcgc                         >  1:1367508/1‑150 (MQ=255)
                                                                                                                             gggATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGCGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCa                        >  2:1733751/1‑150 (MQ=255)
                                                                                                                             gggATTGAGATAAGCCGCCTATGGTAGCCAAAAGGCGCTGATGATTAATAGAAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTATTAATCAGTTGTGATGACGCACAGCGCGCa                        >  2:22914/1‑150 (MQ=255)
                                                                                                                                  tGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAAc                   >  2:671449/1‑150 (MQ=255)
                                                                                                                                    agaTAAGCCGCCTTTGGTAGCCCAAAGGCGGTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCAGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTc                 >  2:118446/1‑150 (MQ=255)
                                                                                                                                     gaTAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCg                <  1:531517/150‑1 (MQ=255)
                                                                                                                                      aTAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGCGCGTGACGTTGTGCTCCCTGGTTCCTGCCTTTTAATCAGTTGTGATGCCGCACAGCGCGCAGAAACTCGt               >  1:1118900/1‑150 (MQ=255)
                                                                                                                                      aTAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGt               >  2:524905/1‑150 (MQ=255)
                                                                                                                                      aTAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGt               >  2:1171599/1‑150 (MQ=255)
                                                                                                                                       tAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTg              >  2:478861/1‑150 (MQ=255)
                                                                                                                                          agcgccTTTGGGCTACCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGgcg           >  1:298303/3‑150 (MQ=255)
                                                                                                                                          agcgccTTTGGGCTACCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGgcg           >  2:1055832/3‑150 (MQ=255)
                                                                                                                                           gcgccTTTGGGCTACCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGgcgc          >  1:79481/2‑150 (MQ=255)
                                                                                                                                              ccTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTa       >  1:754890/1‑150 (MQ=255)
                                                                                                                                                  tGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTGCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCt   <  2:866073/150‑1 (MQ=255)
                                                                                                                                                   ggTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTg  <  1:91780/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GGCAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTG  >  NC_000913/2242735‑2243031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: