Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,242,885 A→C 9.6% L28R (CTA→CGA) ‡ yeiB ← DUF418 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,242,8850AC 9.6% 319.6 / 22.3 115L28R (CTA→CGA) ‡yeiBDUF418 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (56/48);  new base C (6/5);  total (62/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.79e-01

Only 100 of 117 total aligned reads displayed.

CAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACT  >  NC_000913/2242737‑2243034
                                                                                                                                                    |                                                                                                                                                     
cagcaACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGGACCCGGCGGGATTGAGATAAGCCGCCTTTGGTAg                                                                                                                                                      >  1:1093893/1‑150 (MQ=255)
   caACATTTGCACGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGccc                                                                                                                                                   <  2:901023/150‑1 (MQ=255)
    aaCATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGAGTGAGATAAGCCGCCTTTGGTAGcccc                                                                                                                                                  >  1:304801/1‑149 (MQ=255)
        tttGCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAgg                                                                                                                                              <  2:1578876/150‑1 (MQ=255)
         ttGCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGgc                                                                                                                                             <  1:609810/150‑1 (MQ=255)
          tGCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCTAGTCCATGCATCCCGCGGCGTACTCGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGGCTACCAAAGgcg                                                                                                                                            <  2:1043494/150‑1 (MQ=255)
           gCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGgcgc                                                                                                                                           <  1:904816/150‑1 (MQ=255)
            cAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGGCTACCAAAGgcggc                                                                                                                                          <  2:1644420/150‑3 (MQ=255)
            cAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCGCCTTTGGGCTACCAAAGgcggct                                                                                                                                         <  2:574593/150‑4 (MQ=255)
             aGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGACATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTg                                                                                                                                         <  1:1458142/150‑1 (MQ=255)
              ggCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGa                                                                                                                                        >  1:818174/1‑150 (MQ=255)
               gCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGAt                                                                                                                                       <  2:1536790/150‑1 (MQ=255)
                cccGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGGCTACCAAAGgcgg                                                                                                                                           <  2:736786/145‑2 (MQ=255)
                cccGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGGCTACCAAAGgcgg                                                                                                                                           >  1:736786/1‑144 (MQ=255)
                  cGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGtt                                                                                                                                    <  2:4146/150‑1 (MQ=255)
                   gCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGttt                                                                                                                                   <  1:1202496/150‑1 (MQ=255)
                   gCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCAACCCGCGGCTTAATAGCACCGTACCAGGAGGGATTGAGATAAGCCGCCTTTGCTAGCCCAAAGGCGCTGATGttt                                                                                                                                   >  1:205764/1‑150 (MQ=255)
                   gCAACAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGttt                                                                                                                                   <  2:1083419/150‑1 (MQ=255)
                    cACCAAACAGTAGCGCAAAACGCGGGAGGAATTTCCCCTGGCCGAGGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTa                                                                                                                                  >  1:887240/1‑150 (MQ=255)
                    cACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTa                                                                                                                                  <  2:1679515/150‑1 (MQ=255)
                      ccAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCAGGCCGATGAGAACAACAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAAt                                                                                                                                >  1:196549/1‑150 (MQ=255)
                        aaaCAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAG                                                                                                                              >  1:857008‑M1/1‑150 (MQ=255)
                        aaaCAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAG                                                                                                                              <  2:1344549‑M1/150‑1 (MQ=255)
                        aaaCAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCAGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCCCCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAG                                                                                                                              >  1:483393‑M1/1‑150 (MQ=255)
                                gcgcAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATcc                                                                                                                      >  1:1172710/1‑150 (MQ=255)
                                   cAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCa                                                                                                                   <  2:88368/150‑1 (MQ=255)
                                          cGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGc                                                                                                            <  2:815359/150‑1 (MQ=255)
                                           gTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCg                                                                                                           >  1:1482968/1‑150 (MQ=255)
                                             gAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGAc                                                                                                         >  1:1205076/1‑150 (MQ=255)
                                             gAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGAc                                                                                                         >  2:708244/1‑150 (MQ=255)
                                               ggAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGAcgc                                                                                                       >  2:383895/1‑150 (MQ=255)
                                                  aTTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGGACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGAcgccgc                                                                                                    <  1:248570/150‑1 (MQ=255)
                                                   tttCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCcgcg                                                                                                   <  2:731258/150‑1 (MQ=255)
                                                    ttCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGa                                                                                                  >  1:421071/1‑150 (MQ=255)
                                                      cACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAAc                                                                                                <  2:1511963/150‑1 (MQ=255)
                                                      cACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAAc                                                                                                >  2:1547240/1‑150 (MQ=255)
                                                           ggCCGATGAGATCAAGAAATGCCCAAGTCCATGCTTCCGGGGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCCGTTTCCCCAGAATGGCGACGCCGCGAACAAAAt                                                                                           <  1:268143/150‑1 (MQ=255)
                                                              cGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGAaccatggagcgcaacgtcacgctcgattttgtt                                                                                        >  1:1524258‑M1/1‑117 (MQ=255)
                                                               gATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGAAGCCCAAAGGCGCTGATGTTTATTAGCAGGATCCCCAGAATGGCGACGCCGCGCACAAAATCGAg                                                                                       >  2:305941/1‑150 (MQ=255)
                                                                aTGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGc                                                                                      <  1:643188/150‑1 (MQ=255)
                                                                 tGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCGGATGTTTAATAGCAGGCTCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCg                                                                                     >  1:1486755/1‑150 (MQ=255)
                                                                  gagaTCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGt                                                                                    >  2:1034026/1‑150 (MQ=255)
                                                                   agaTCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTg                                                                                   >  1:991323/1‑150 (MQ=255)
                                                                   agaTCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTg                                                                                   >  2:636387/1‑150 (MQ=255)
                                                                         aGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTg                                                                             <  1:919831/150‑1 (MQ=255)
                                                                         aGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTg                                                                             <  2:951288/150‑1 (MQ=255)
                                                                         aGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTg                                                                             <  1:551695/150‑1 (MQ=255)
                                                                            aaTGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTgcgc                                                                          >  2:712082/1‑150 (MQ=255)
                                                                                 ccAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCAt                                                                     <  1:1226600/150‑1 (MQ=255)
                                                                                 ccAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCAt                                                                     >  1:651238/1‑150 (MQ=255)
                                                                                   aaGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATgg                                                                   <  2:421071/150‑1 (MQ=255)
                                                                                    aGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGCGCGTGACGTTGCGCTCCATGGt                                                                  <  1:708244/150‑1 (MQ=255)
                                                                                     gTCCATGCATCGCTCGGCGTAATAGCACCGTACCAGGCGGGATTGAGTTAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGTGCGTGACGTTGCGCTCCATGGTt                                                                 >  1:1071477‑M2/1‑149 (MQ=255)
                                                                                       ccATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGAaccatggagcgcaacgtcacgctcgattttgttcgcggcgtcgccattctggggatcc                                                               <  2:1524258‑M1/150‑59 (MQ=255)
                                                                                       ccATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGACCAAAATCGAGCGTGACGTTGCGCTCCATGGTtcc                                                               <  1:1675876‑M2/150‑4 (MQ=255)
                                                                                       ccATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTtcc                                                               >  1:56748‑M2/1‑147 (MQ=255)
                                                                                        cATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGAGTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGAGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTtcct                                                              <  2:73446‑M2/150‑5 (MQ=255)
                                                                                           gCATCCCGCGGCGTCATAGCACCGTACCAGGCGGGATTGAGATAACCCGCCTTTGGTAGCCCAACGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGcc                                                           <  1:311224/150‑1 (MQ=255)
                                                                                           gCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGcc                                                           <  2:929613/150‑1 (MQ=255)
                                                                                            cATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCt                                                          >  2:1598419/1‑150 (MQ=255)
                                                                                              tCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCttt                                                        <  1:827976/150‑1 (MQ=255)
                                                                                              tCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCttt                                                        <  2:1671482/150‑1 (MQ=255)
                                                                                              gcccGCGGCTTAAGAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCGTTGGGAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGCAGGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGGTCCTGCCttt                                                        <  1:1060353/149‑1 (MQ=255)
                                                                                                ccGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTa                                                      <  2:1172710/150‑1 (MQ=255)
                                                                                                ccGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTa                                                      >  1:263941/1‑150 (MQ=255)
                                                                                                 cgcgGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGAaccatggagcgcaacgtcacgctcgattttgttcgcggcgtcgccattctggggatcctgctattaaa                                                     <  1:1546632‑M1/150‑69 (MQ=255)
                                                                                                     gcgTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAg                                                 >  1:1700537/1‑150 (MQ=255)
                                                                                                      cgTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGt                                                <  2:1562430/150‑1 (MQ=255)
                                                                                                       gTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACTAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGtt                                               >  1:106720/1‑150 (MQ=255)
                                                                                                             ccACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATTTTTAATAGCAGGTTCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGGGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGtgatg                                         <  2:345875/149‑1 (MQ=255)
                                                                                                              cACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGtgatga                                        >  2:1397329/1‑150 (MQ=255)
                                                                                                              cACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGtgatga                                        >  2:91780/1‑150 (MQ=255)
                                                                                                               aCCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGAc                                       <  1:212593/150‑1 (MQ=255)
                                                                                                                ccGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGAAGTTTAAAAGCAGGATCACCAGAATGGAGACGCCGCGAACAAAATAGAGCGTGTCGTTGCGCTCCATGGTTCATGCCGTTTAATCAGTTGTGATGACg                                      >  1:629633/1‑150 (MQ=255)
                                                                                                                 cGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGc                                     <  2:857008/150‑1 (MQ=255)
                                                                                                                  gTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATAAGTTGTGATGACGca                                    >  1:1286349/1‑150 (MQ=255)
                                                                                                                   tACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGcac                                   >  1:738865/1‑150 (MQ=255)
                                                                                                                       aGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCACAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAgcg                               <  2:1093893/150‑1 (MQ=255)
                                                                                                                       aGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAgcg                               >  1:1505234/1‑150 (MQ=255)
                                                                                                                         gCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAgcgcg                             <  1:1547240/150‑1 (MQ=255)
                                                                                                                          cGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAgcgcgc                            >  1:1367508/1‑150 (MQ=255)
                                                                                                                           gggATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGCGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCa                           >  2:1733751/1‑150 (MQ=255)
                                                                                                                           gggATTGAGATAAGCCGCCTATGGTAGCCAAAAGGCGCTGATGATTAATAGAAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTATTAATCAGTTGTGATGACGCACAGCGCGCa                           >  2:22914/1‑150 (MQ=255)
                                                                                                                                tGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAAc                      >  2:671449/1‑150 (MQ=255)
                                                                                                                                  agaTAAGCCGCCTTTGGTAGCCCAAAGGCGGTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCAGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTc                    >  2:118446/1‑150 (MQ=255)
                                                                                                                                   gaTAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCg                   <  1:531517/150‑1 (MQ=255)
                                                                                                                                    aTAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGCGCGTGACGTTGTGCTCCCTGGTTCCTGCCTTTTAATCAGTTGTGATGCCGCACAGCGCGCAGAAACTCGt                  >  1:1118900/1‑150 (MQ=255)
                                                                                                                                    aTAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGt                  >  2:524905/1‑150 (MQ=255)
                                                                                                                                    aTAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGt                  >  2:1171599/1‑150 (MQ=255)
                                                                                                                                     tAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTg                 >  2:478861/1‑150 (MQ=255)
                                                                                                                                        agcgccTTTGGGCTACCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGgcg              >  1:298303/3‑150 (MQ=255)
                                                                                                                                        agcgccTTTGGGCTACCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGgcg              >  1:216670/3‑150 (MQ=255)
                                                                                                                                        agcgccTTTGGGCTACCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGgcg              >  2:1055832/3‑150 (MQ=255)
                                                                                                                                         gcgccTTTGGGCTACCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGCCGCACAGCGCGCAGAAACTCGTGgcgc             >  1:575354/2‑150 (MQ=255)
                                                                                                                                         gcgccTTTGGGCTACCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGgcgc             >  1:79481/2‑150 (MQ=255)
                                                                                                                                            ccTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTa          >  1:754890/1‑150 (MQ=255)
                                                                                                                                                tGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTGCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCt      <  2:866073/150‑1 (MQ=255)
                                                                                                                                                 ggaaGCCCAAAGGCGCTGATGTTTACTAGCAGGAGCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTACTCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTg     <  1:64334/147‑1 (MQ=255)
                                                                                                                                                 ggTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTg     <  1:91780/150‑1 (MQ=255)
                                                                                                                                                    aGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACt  >  2:1238634/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACT  >  NC_000913/2242737‑2243034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: