Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,242,887 C→A 8.9% G27G (GGG→GGT yeiB ← DUF418 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,242,8870CA 8.9% 330.7 / 13.3 113G27G (GGG→GGTyeiBDUF418 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (55/47);  new base A (6/5);  total (61/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

GCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACT  >  NC_000913/2242739‑2243034
                                                                                                                                                    |                                                                                                                                                   
gcaACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGcc                                                                                                                                                    <  2:1493041/150‑1 (MQ=255)
 caACATTTGCACGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGccc                                                                                                                                                   <  2:901023/150‑1 (MQ=255)
  aaCATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGAGTGAGATAAGCCGCCTTTGGTAGcccc                                                                                                                                                  >  1:304801/1‑149 (MQ=255)
      tttGCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGCTGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAgg                                                                                                                                              <  1:954021/150‑1 (MQ=255)
      tttGCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAgg                                                                                                                                              <  2:1578876/150‑1 (MQ=255)
       ttGCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGgc                                                                                                                                             <  1:609810/150‑1 (MQ=255)
        tGCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCTAGTCCATGCATCCCGCGGCGTACTCGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGGCTACCAAAGgcg                                                                                                                                            <  2:1043494/150‑1 (MQ=255)
          cAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGGCTACCAAAGgcggc                                                                                                                                          <  1:589845/150‑3 (MQ=255)
          cAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGGCTACCAAAGgcggc                                                                                                                                          <  2:1644420/150‑3 (MQ=255)
          cAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCGCCTTTGGGCTACCAAAGgcggct                                                                                                                                         <  2:574593/150‑4 (MQ=255)
           aGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGACATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTg                                                                                                                                         <  1:1458142/150‑1 (MQ=255)
            ggCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGa                                                                                                                                        >  1:818174/1‑150 (MQ=255)
             gCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGAt                                                                                                                                       <  2:1536790/150‑1 (MQ=255)
              cccGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATg                                                                                                                                      >  1:720942/1‑150 (MQ=255)
              cccGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGGCTACCAAAGgcgg                                                                                                                                           >  1:736786/1‑144 (MQ=255)
              cccGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGGCTACCAAAGgcgg                                                                                                                                           <  2:736786/145‑2 (MQ=255)
                cGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGtt                                                                                                                                    <  2:4146/150‑1 (MQ=255)
                 gCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGttt                                                                                                                                   <  1:1202496/150‑1 (MQ=255)
                 gCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCAACCCGCGGCTTAATAGCACCGTACCAGGAGGGATTGAGATAAGCCGCCTTTGCTAGCCCAAAGGCGCTGATGttt                                                                                                                                   >  1:205764/1‑150 (MQ=255)
                  cACCAAACAGTAGCGCAAAACGCGGGAGGAATTTCCCCTGGCCGAGGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTa                                                                                                                                  >  1:887240/1‑150 (MQ=255)
                  cACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTa                                                                                                                                  <  2:1679515/150‑1 (MQ=255)
                    ccAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCAGGCCGATGAGAACAACAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAAt                                                                                                                                >  1:196549/1‑150 (MQ=255)
                      aaaCAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAG                                                                                                                              >  1:857008‑M1/1‑150 (MQ=255)
                      aaaCAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCAGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCCCCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAG                                                                                                                              >  1:483393‑M1/1‑150 (MQ=255)
                          aGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGG                                                                                                                          >  1:241246‑M1/1‑150 (MQ=255)
                              gcgcAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATcc                                                                                                                      >  2:1118666/1‑150 (MQ=255)
                              gcgcAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATcc                                                                                                                      >  1:1172710/1‑150 (MQ=255)
                               cgcAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATccc                                                                                                                     >  1:850739/1‑150 (MQ=255)
                                 cAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCa                                                                                                                   <  2:88368/150‑1 (MQ=255)
                                        cGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGc                                                                                                            <  2:815359/150‑1 (MQ=255)
                                         gTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCg                                                                                                           >  1:1482968/1‑150 (MQ=255)
                                           gAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGAc                                                                                                         >  2:708244/1‑150 (MQ=255)
                                             ggAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGAcgc                                                                                                       >  2:383895/1‑150 (MQ=255)
                                                aTTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGGACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGAcgccgc                                                                                                    <  1:248570/150‑1 (MQ=255)
                                                 tttCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCcgcg                                                                                                   <  2:731258/150‑1 (MQ=255)
                                                 tttCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCcgcg                                                                                                   <  2:233365/150‑1 (MQ=255)
                                                  ttCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGa                                                                                                  >  1:421071/1‑150 (MQ=255)
                                                    cACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAAc                                                                                                <  2:1511963/150‑1 (MQ=255)
                                                    cACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAAc                                                                                                >  2:1547240/1‑150 (MQ=255)
                                                         ggCCGATGAGATCAAGAAATGCCCAAGTCCATGCTTCCGGGGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCCGTTTCCCCAGAATGGCGACGCCGCGAACAAAAt                                                                                           <  1:268143/150‑1 (MQ=255)
                                                            cGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGAaccatggagcgcaacgtcacgctcgattttgtt                                                                                        >  1:1524258‑M1/1‑117 (MQ=255)
                                                             gATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGAAGCCCAAAGGCGCTGATGTTTATTAGCAGGATCCCCAGAATGGCGACGCCGCGCACAAAATCGAg                                                                                       >  2:305941/1‑150 (MQ=255)
                                                                gagaTCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGt                                                                                    >  2:1034026/1‑150 (MQ=255)
                                                                 agaTCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTg                                                                                   >  1:991323/1‑150 (MQ=255)
                                                                 agaTCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTg                                                                                   >  2:636387/1‑150 (MQ=255)
                                                                       aGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTg                                                                             <  2:951288/150‑1 (MQ=255)
                                                                       aGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTg                                                                             <  1:919831/150‑1 (MQ=255)
                                                                       aGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTg                                                                             <  1:551695/150‑1 (MQ=255)
                                                                             gCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCGGATGTTTAATAGCCGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTcc                                                                       >  2:870798/1‑150 (MQ=255)
                                                                               ccAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCAt                                                                     <  1:1226600/150‑1 (MQ=255)
                                                                               ccAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCAt                                                                     >  1:651238/1‑150 (MQ=255)
                                                                                 aaGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATgg                                                                   <  2:421071/150‑1 (MQ=255)
                                                                                  aGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGCGCGTGACGTTGCGCTCCATGGt                                                                  <  1:708244/150‑1 (MQ=255)
                                                                                   gTCCATGCATCGCTCGGCGTAATAGCACCGTACCAGGCGGGATTGAGTTAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGTGCGTGACGTTGCGCTCCATGGTt                                                                 >  1:1071477‑M2/1‑149 (MQ=255)
                                                                                    tCCATGCATCCCGCGGCTTAATAGCACCGTACCAGGCTGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGTCGCCGCGAACAATATCGAGCGTGACGTTGCGCTCCATGGTtc                                                                >  1:1568963‑M2/1‑148 (MQ=255)
                                                                                     ccATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGAaccatggagcgcaacgtcacgctcgattttgttcgcggcgtcgccattctggggatcc                                                               <  2:1524258‑M1/150‑59 (MQ=255)
                                                                                     ccATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGACCAAAATCGAGCGTGACGTTGCGCTCCATGGTtcc                                                               <  1:1675876‑M2/150‑4 (MQ=255)
                                                                                     ccATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTtcc                                                               >  1:56748‑M2/1‑147 (MQ=255)
                                                                                      cATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGAGTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGAGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTtcct                                                              <  2:73446‑M2/150‑5 (MQ=255)
                                                                                         gCATCCCGCGGCGTCATAGCACCGTACCAGGCGGGATTGAGATAACCCGCCTTTGGTAGCCCAACGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGcc                                                           <  1:311224/150‑1 (MQ=255)
                                                                                         gCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGcc                                                           <  1:34347/150‑1 (MQ=255)
                                                                                         gCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGcc                                                           <  2:929613/150‑1 (MQ=255)
                                                                                          cATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCt                                                          >  2:1598419/1‑150 (MQ=255)
                                                                                            tCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCttt                                                        <  1:827976/150‑1 (MQ=255)
                                                                                            tCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCttt                                                        <  2:1671482/150‑1 (MQ=255)
                                                                                            gcccGCGGCTTAAGAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCGTTGGGAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGCAGGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGGTCCTGCCttt                                                        <  1:1060353/149‑1 (MQ=255)
                                                                                              ccGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTa                                                      <  2:1172710/150‑1 (MQ=255)
                                                                                               cgcgGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGAaccatggagcgcaacgtcacgctcgattttgttcgcggcgtcgccattctggggatcctgctattaaa                                                     <  1:1546632‑M1/150‑69 (MQ=255)
                                                                                                     gTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACTAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGtt                                               >  1:106720/1‑150 (MQ=255)
                                                                                                       aaTAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTATGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGACGCCAACAAAATCGAGCTTGAAGTTGCGCTCCATGGTTCCTGCCTTATAATCAGAtga                                             >  2:1598385/1‑149 (MQ=255)
                                                                                                           ccACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATTTTTAATAGCAGGTTCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGGGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGtgatg                                         <  2:345875/149‑1 (MQ=255)
                                                                                                           ccACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGtgatg                                         <  2:731065/149‑1 (MQ=255)
                                                                                                            cACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGtgatga                                        >  2:91780/1‑150 (MQ=255)
                                                                                                            cACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGtgatga                                        >  2:1397329/1‑150 (MQ=255)
                                                                                                             aCCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGAc                                       <  1:212593/150‑1 (MQ=255)
                                                                                                              ccGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGAAGTTTAAAAGCAGGATCACCAGAATGGAGACGCCGCGAACAAAATAGAGCGTGTCGTTGCGCTCCATGGTTCATGCCGTTTAATCAGTTGTGATGACg                                      >  1:629633/1‑150 (MQ=255)
                                                                                                               cGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGc                                     <  2:857008/150‑1 (MQ=255)
                                                                                                                gTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATAAGTTGTGATGACGca                                    >  1:1286349/1‑150 (MQ=255)
                                                                                                                 tACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGcac                                   >  1:738865/1‑150 (MQ=255)
                                                                                                                 gtacAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTAAATCAGTTGTGATGACGcac                                   >  1:1471597/4‑150 (MQ=255)
                                                                                                                     aGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCACAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAgcg                               <  2:1093893/150‑1 (MQ=255)
                                                                                                                     aGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAgcg                               >  1:1505234/1‑150 (MQ=255)
                                                                                                                       gCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAgcgcg                             <  1:1547240/150‑1 (MQ=255)
                                                                                                                        cGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAgcgcgc                            >  1:1367508/1‑150 (MQ=255)
                                                                                                                         gggATTGAGATAAGCCGCCTATGGTAGCCAAAAGGCGCTGATGATTAATAGAAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTATTAATCAGTTGTGATGACGCACAGCGCGCa                           >  2:22914/1‑150 (MQ=255)
                                                                                                                              tGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAAc                      >  2:671449/1‑150 (MQ=255)
                                                                                                                                agaTAAGCCGCCTTTGGTAGCCCAAAGGCGGTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCAGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTc                    >  2:118446/1‑150 (MQ=255)
                                                                                                                                  aTAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGCGCGTGACGTTGTGCTCCCTGGTTCCTGCCTTTTAATCAGTTGTGATGCCGCACAGCGCGCAGAAACTCGt                  >  1:1118900/1‑150 (MQ=255)
                                                                                                                                  aTAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGt                  >  2:1171599/1‑150 (MQ=255)
                                                                                                                                  aTAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGt                  >  2:524905/1‑150 (MQ=255)
                                                                                                                                   tAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTg                 >  2:48593/1‑150 (MQ=255)
                                                                                                                                      agcgccTTTGGGCTACCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGgcg              >  1:216670/3‑150 (MQ=255)
                                                                                                                                      agcgccTTTGGGCTACCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGgcg              >  2:1055832/3‑150 (MQ=255)
                                                                                                                                      agcgccTTTGGGCTACCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGgcg              >  1:298303/3‑150 (MQ=255)
                                                                                                                                       gcgccTTTGGGCTACCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGCCGCACAGCGCGCAGAAACTCGTGgcgc             >  1:575354/2‑150 (MQ=255)
                                                                                                                                       gcgccTTTGGGCTACCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGgcgc             >  1:79481/2‑150 (MQ=255)
                                                                                                                                        cgccTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGcgcg            >  1:118377/1‑150 (MQ=255)
                                                                                                                                               ggaaGCCCAAAGGCGCTGATGTTTACTAGCAGGAGCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTACTCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTg     <  1:64334/147‑1 (MQ=255)
                                                                                                                                               ggTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTg     <  1:91780/150‑1 (MQ=255)
                                                                                                                                                  aGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACt  >  2:1238634/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACT  >  NC_000913/2242739‑2243034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: