Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,247,030 G→A 7.3% intergenic (‑261/+33) cirA ← / ← lysP colicin IA outer membrane receptor and translocator; ferric iron‑catecholate transporter/lysine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,247,0300GA 7.3% 368.1 / 11.5 123intergenic (‑261/+33)cirA/lysPcolicin IA outer membrane receptor and translocator; ferric iron‑catecholate transporter/lysine transporter
Reads supporting (aligned to +/- strand):  ref base G (47/67);  new base A (6/3);  total (53/70)
Fisher's exact test for biased strand distribution p-value = 1.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.37e-01

Only 100 of 125 total aligned reads displayed.

CATCCCAGAAAGCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGC  >  NC_000913/2246882‑2247179
                                                                                                                                                     |                                                                                                                                                     
catcCCAGAAAGCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCgg                                                                                                                                                      <  1:685786/150‑1 (MQ=255)
 atcCCAGAAAGCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGt                                                                                                                                                     <  2:778515/150‑1 (MQ=255)
  tcCCAGAAAGCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGtt                                                                                                                                                    >  2:579069/1‑150 (MQ=255)
  tcCCAGAAAGCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGtt                                                                                                                                                    <  2:1347329/150‑1 (MQ=255)
   cccAGAAAGCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTg                                                                                                                                                   <  2:1187984/150‑1 (MQ=255)
   cccAGAAAGCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTg                                                                                                                                                   <  2:434464/150‑1 (MQ=255)
   cccAGAAAGCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTg                                                                                                                                                   <  1:1651018/150‑1 (MQ=255)
      aGAAAGCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTgaga                                                                                                                                                <  1:579268/150‑1 (MQ=255)
          aGCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGc                                                                                                                                            <  1:553022/150‑1 (MQ=255)
           gCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCt                                                                                                                                           >  1:1531421/1‑150 (MQ=255)
           gCCGGAACAGCCTATGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCt                                                                                                                                           <  2:604549/150‑1 (MQ=255)
            ccGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCtt                                                                                                                                          >  2:105226/1‑150 (MQ=255)
               gAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGc                                                                                                                                       <  2:834151/150‑1 (MQ=255)
                aaCAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCtttttc                                                                                                                                      <  1:1699937/150‑5 (MQ=255)
                aaCAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCAACCGAGAGGGCtttttc                                                                                                                                      <  1:692712/150‑5 (MQ=255)
                 aCAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCa                                                                                                                                      <  1:1672410/150‑1 (MQ=255)
                 aCAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGATTAGCaa                                                                                                                                     >  2:452506/1‑150 (MQ=255)
                  cAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACTATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCaag                                                                                                                                    <  2:398196/150‑1 (MQ=255)
                  cAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCaag                                                                                                                                    <  2:144962/150‑1 (MQ=255)
                    gCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCaagga                                                                                                                                  >  2:800267/1‑150 (MQ=255)
                    gCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCaagga                                                                                                                                  >  2:621446/1‑150 (MQ=255)
                     ccTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCaaggaa                                                                                                                                 >  2:174065/1‑150 (MQ=255)
                        ctGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAggg                                                                                                                              >  2:774467/1‑150 (MQ=255)
                         tGATTTGTCAGCCTTTTTTGATTGCGGAGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGgga                                                                                                                             <  2:412385/150‑1 (MQ=255)
                          gATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGggag                                                                                                                            <  1:1018135/150‑1 (MQ=255)
                           aTTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGggagg                                                                                                                           <  1:621446/150‑1 (MQ=255)
                             ttGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGaa                                                                                                                         <  1:1621501/150‑1 (MQ=255)
                               gTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGaaaa                                                                                                                       >  2:1210940/1‑149 (MQ=255)
                                 cAGCCTTTTTTGATAGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACtt                                                                                                                     >  2:1340150/1‑150 (MQ=255)
                                    cattttttGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTAtt                                                                                                                  <  1:326513/148‑1 (MQ=255)
                                     cTTTTTTGATTGCGGCGTAACGAGAATGCAACCGATACCAATTCTCAAGCCAAAGGTTAAATTTTATATCCGCATGATTTGTCAGGGAAAGGGAATTGAAAAAGCCCTCTAGTTTGAGAGGGCGTAGCAAGGAAGGGAGTACACTTAatt                                                                                                                 >  2:858573/1‑150 (MQ=255)
                                      ttttttGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTc                                                                                                                <  2:1085802/150‑1 (MQ=255)
                                      ttttttGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGACAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTc                                                                                                                >  1:1392565/1‑150 (MQ=255)
                                       tttttGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCt                                                                                                               <  2:451623/150‑1 (MQ=255)
                                         tttGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTa                                                                                                             <  1:30818/150‑1 (MQ=255)
                                           tGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATc                                                                                                           >  2:498425/1‑150 (MQ=255)
                                            gATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCg                                                                                                          <  1:1613900/150‑1 (MQ=255)
                                                      tAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGa                                                                                                >  2:960468/1‑150 (MQ=255)
                                                      tAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATAGAAAAAGCCATCACGGTTCAGAGGGCTAAGCAAGGAATGGAGCAAAATTATTTCTTAACGTTCTGCGGGa                                                                                                >  2:517399/1‑150 (MQ=255)
                                                      tAACGAAAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAAAGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGa                                                                                                <  2:1100926/150‑1 (MQ=255)
                                                        aCGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAAc                                                                                              <  1:694040/150‑1 (MQ=255)
                                                         cGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACt                                                                                             <  1:611626/150‑1 (MQ=255)
                                                          gATAATGCAAACGATAACAATTATCAATCCAAATGTTAGATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACtt                                                                                            <  1:504814/150‑1 (MQ=255)
                                                          gATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGATGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACtt                                                                                            >  2:564664/1‑150 (MQ=255)
                                                           aTAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTc                                                                                           >  2:1520007/1‑150 (MQ=255)
                                                                gCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTc                                                                                      <  1:573550/150‑1 (MQ=255)
                                                                 cAAGCGATAACAATTATCAATCCAAATGTTAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGc                                                                                    >  2:94103/1‑150 (MQ=255)
                                                                 cAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCg                                                                                     <  1:786218/150‑1 (MQ=255)
                                                                  aaaCGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGc                                                                                    >  1:925709/1‑150 (MQ=255)
                                                                  aaaCGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGc                                                                                    >  1:622141/1‑150 (MQ=255)
                                                                    aCGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTg                                                                                  >  1:579711/1‑150 (MQ=255)
                                                                     cGATAACTATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAATTGTAATTGAAAAAGCGCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGt                                                                                 <  2:255595/150‑1 (MQ=255)
                                                                       taaaCAATTATAAATACAAAAGGTAAAATTTACTTCCGCATTCTTTTGCACGGAAATGGAAATTAAAAAGCCCTCACGGTTGTGCTGGGCTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAg                                                                               <  1:733789/148‑1 (MQ=255)
                                                                         aaCAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCg                                                                             >  2:1034000/1‑150 (MQ=255)
                                                                          aCAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGATAGGGCTTAGCAAGGAAGGGAGGAAACTTATATCTTATCTTTCTGCGGGAACTTCATTTCGCTGTAGctt                                                                            >  2:1300676/1‑148 (MQ=255)
                                                                           cAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTa                                                                           >  2:905555/1‑150 (MQ=255)
                                                                            aaTTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTAc                                                                          <  2:1425199/150‑1 (MQ=255)
                                                                             aTTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACg                                                                         <  2:1484436/150‑1 (MQ=255)
                                                                               tATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGaa                                                                       >  1:1209403/1‑150 (MQ=255)
                                                                                    aTCCAAATTTTAAAATTTATATCCGGATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAg                                                                  <  1:41237/150‑1 (MQ=255)
                                                                                       cAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGGTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTc                                                               <  1:1554794/150‑1 (MQ=255)
                                                                                          aTGTTAAATTTTATATCCGCATGATTTGTCTGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCtt                                                            <  1:248667/150‑1 (MQ=255)
                                                                                          aTGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCtt                                                            <  2:1349230/150‑1 (MQ=255)
                                                                                          aTGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCtt                                                            <  2:1218553/150‑1 (MQ=255)
                                                                                            gTTAAATTTTATATCCGCATGATTTGACAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTa                                                          <  2:1392565/150‑1 (MQ=255)
                                                                                             ttAAATTTTATATCCTCATGATTTTTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTaa                                                         <  1:930097/150‑1 (MQ=255)
                                                                                             ttAAATTTTATATCCGCATTATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGGTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGTGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTaa                                                         <  1:1222329/150‑1 (MQ=255)
                                                                                                 aTTTTATATCCGCATGATTTGACAGGGAAATTGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGTAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGAACGAAGTGAGTTCCTTTAATCac                                                     >  2:389226/1‑149 (MQ=255)
                                                                                                   tttATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCt                                                   <  1:1415858/150‑1 (MQ=255)
                                                                                                    ttATATCCTAATGATTTTTCAGTGAATTTTAATTGAAAAAGCACTATCGGGTTAGAGGGCTTAGCAAGGAAGGTAGGAAACTTATTTCTTATCGTTATGCTGGAACTTCATTTAGCTGTAGCGTACTAAGTGAGTTCCTTTAATCAGCtt                                                  <  1:1559511/150‑1 (MQ=255)
                                                                                                      atatCCGGAGGATTTGGCACGGAAATGGAATTGAAACAGCCCTCTGGGTTGAGAGGGCTTATCTATGAAGGGTGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGt                                                <  1:1068496/150‑1 (MQ=255)
                                                                                                        atCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAg                                              >  1:798859/1‑150 (MQ=255)
                                                                                                         tCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGc                                             <  1:473079/150‑1 (MQ=255)
                                                                                                           cGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCg                                           >  1:800726/1‑150 (MQ=255)
                                                                                                              aTGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAAc                                        >  2:514281/1‑150 (MQ=255)
                                                                                                               tGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAAcc                                       >  2:40378/1‑150 (MQ=255)
                                                                                                                     gTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAaataa                                 >  1:1023723/1‑150 (MQ=255)
                                                                                                                     gTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAaataa                                 >  2:1707011/1‑150 (MQ=255)
                                                                                                                     gTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAaataa                                 >  1:620112/1‑150 (MQ=255)
                                                                                                                       cAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATATCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATc                               >  2:651780/1‑150 (MQ=255)
                                                                                                                          ggAAATGGATTTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAgg                            <  2:554033/150‑1 (MQ=255)
                                                                                                                          ggAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAgg                            >  1:1138925/1‑150 (MQ=255)
                                                                                                                           taaaTGTAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGCGAACATAATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGa                           <  1:93798/149‑1 (MQ=255)
                                                                                                                             aaTGGAATTTAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAAc                         <  1:222731/150‑1 (MQ=255)
                                                                                                                             aaTGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAAc                         <  2:525995/150‑1 (MQ=255)
                                                                                                                             aaTGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTGCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAAc                         <  1:1324685/150‑1 (MQ=255)
                                                                                                                                ggAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGc                      <  1:458997/150‑1 (MQ=255)
                                                                                                                                 gAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCg                     >  2:51463/1‑150 (MQ=255)
                                                                                                                                  aaTTGAACAAGCCCTCTCGCGTGAGAGGGCGTAGCAAGGAAGGGAGGAAACTTATTTCATATCGTTCTGCGGGAACTTCATTTCGCTGGAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCgg                    <  2:255392/150‑1 (MQ=255)
                                                                                                                                   aTTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGATCCTTTAATCAGCTTGTAGCCGAACCAAATAATCATGAACTGCggg                   >  2:302031/1‑150 (MQ=255)
                                                                                                                                       tgctaaGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATa                >  1:1342125/5‑150 (MQ=255)
                                                                                                                                        gctaaGCCCTCTCAACCGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATAc               >  1:914878/4‑150 (MQ=255)
                                                                                                                                        gctaaGCCCTCTCAACCGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGAAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATAc               >  2:1283582/4‑150 (MQ=255)
                                                                                                                                         ctaaGCCCTCTCAACCGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGCACAGCGGGATAcc              >  2:1587352/3‑150 (MQ=255)
                                                                                                                                         ctaaGCCCTCTCAACCGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATAcc              >  1:96527/3‑150 (MQ=255)
                                                                                                                                         aaaaGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATAcc              <  2:1300551/150‑1 (MQ=255)
                                                                                                                                            aGCCCTCTCAACCGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATACCAat           >  1:1249560/1‑150 (MQ=255)
                                                                                                                                                 tctcGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACg      <  1:105226/150‑1 (MQ=255)
                                                                                                                                                  ctcGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGt     >  2:1175634/1‑150 (MQ=255)
                                                                                                                                                     ggTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGc  <  2:622141/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CATCCCAGAAAGCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGC  >  NC_000913/2246882‑2247179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: