Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 252,589 G→C 7.6% A97P (GCA→CCA)  yafO → mRNA interferase toxin of the YafO‑YafN toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913252,5890GC 7.6% 428.4 / 16.5 137A97P (GCA→CCA) yafOmRNA interferase toxin of the YafO‑YafN toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base G (73/53);  new base C (8/3);  total (81/56)
Fisher's exact test for biased strand distribution p-value = 5.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

CCTTTACCTGGCCATTAATCAAATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGAATTTATATTATGAATAACATACAAATAAGAAACTATCA  >  NC_000913/252440‑252737
                                                                                                                                                     |                                                                                                                                                    
ccTTTACCTGGCCATTAATCAAATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGagcaag                                                                                                                                                      >  1:1649113/1‑150 (MQ=255)
ccTTAACCTGGCCATTAATAAAATTTTAGCTAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCATAAGAACGAATGACGAAGCGCATTTAGTATATTGTCAGGGCGCGTTTGATGagaaag                                                                                                                                                      >  1:191059/1‑150 (MQ=255)
 cTTTACCTGGCCATTAATCAAATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGagcaagc                                                                                                                                                     >  1:769788/1‑150 (MQ=255)
   ttACCTGGCCATTAATCAAATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCAt                                                                                                                                                   <  2:768509/150‑1 (MQ=255)
    tACCTGGCCATTAATCAAATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATg                                                                                                                                                  <  1:1545524/150‑1 (MQ=255)
      ccTGGCCATTAATCAAATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGt                                                                                                                                                >  2:1203784/1‑150 (MQ=255)
      ccTGGCCATTAATCAAATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGt                                                                                                                                                <  1:1670581/150‑1 (MQ=255)
      ccTGGCCATTAATCAAATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTAAGGGGGCTTTTGATGAGCAAGCATGGt                                                                                                                                                >  2:270189/1‑150 (MQ=255)
         ggCCATTAATCAAATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGc                                                                                                                                             >  1:817227/1‑150 (MQ=255)
         ggCCATTAATCAAATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGc                                                                                                                                             >  1:1502165/1‑150 (MQ=255)
              ttAATCAAATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCAtt                                                                                                                                        <  2:672686/150‑1 (MQ=255)
               tAATCAAATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACCGTTGCGCCACTTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTg                                                                                                                                       <  2:1056922/150‑1 (MQ=255)
               tAATCAAATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTg                                                                                                                                       <  2:1340127/150‑1 (MQ=255)
                 aTCAAATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTTGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGcc                                                                                                                                     >  1:1549255/1‑150 (MQ=255)
                 aTCAAATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGcc                                                                                                                                     <  1:1651863/150‑1 (MQ=255)
                 aTCAAATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGcc                                                                                                                                     >  1:1463740/1‑150 (MQ=255)
                 aTCAAATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGcc                                                                                                                                     >  2:449603/1‑150 (MQ=255)
                  tCAAATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCATTTCAGCAGAACGCATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCCTTGcct                                                                                                                                    >  2:269379/1‑149 (MQ=255)
                   cAAATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCAt                                                                                                                                   >  2:724749/1‑150 (MQ=255)
                   aaaaTTTGAGCGAGTAGCTCATATTCATCTGGCAAATGAGAATATTCCAATTCCGCCACAGTTGCGCAAATTCAGCAGAACGAATGACGAATCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAtcaaacg                                                                                                                                   <  1:145093/149‑7 (MQ=255)
                                  tGCTGATATTCATCTGGCAAATGAGAATAATCCATTTCCGCAACAGTTGCGCCAATTCAGCAGAACGTATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAAcc                                                                                                                    <  2:1145652/150‑1 (MQ=255)
                                    cTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGTCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTc                                                                                                                  >  2:1451988/1‑150 (MQ=255)
                                    cTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAATAGCGCCAAATCAGCAGAACGCAACACGCCGCGCATTAGCTATAAAGTCCGGGGGCGTTAGATCAGCCAGCAAGGTTACTCATTGCCATTCAGCACCCTGACCCTc                                                                                                                  >  2:1210593/1‑150 (MQ=255)
                                      cATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGACCCTCAt                                                                                                                >  1:605165/1‑150 (MQ=255)
                                       atatTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATa                                                                                                               <  2:504039/150‑1 (MQ=255)
                                            cATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTg                                                                                                          >  1:519329/1‑150 (MQ=255)
                                            cATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTCCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGTGGGCGTTTGATGAGCAAGCAAGGTTGCTCATAGCGATTCTGAAACCTGAACCTCATAAAcag                                                                                                          >  1:276924/1‑148 (MQ=255)
                                            cATCTGGAAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGAGCATTTGGTATATTGTCAGCGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGACATTATGATAACTCAACCTCATAAACtt                                                                                                          >  2:518277/1‑149 (MQ=255)
                                             aTCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTgg                                                                                                         >  1:1556070/1‑150 (MQ=255)
                                              tCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGc                                                                                                        <  2:1212333/150‑1 (MQ=255)
                                               cTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCt                                                                                                       <  1:449603/150‑1 (MQ=255)
                                                 ggCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGATCCTCATAAACTGGCTCg                                                                                                     >  2:37883/1‑150 (MQ=255)
                                                 ggCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTAAGCAGAACGAATGACGAAGCTCATTTGGTAGATTGTCAGGGGGCGTTTGATGCGCAAGTATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCg                                                                                                     >  1:842866/1‑150 (MQ=255)
                                                   cAAATGAGAATAATCCATTTGCGCGACAGTTGCGGCAATTCAGCAGAACGAATGACGATGCGCTTTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCgag                                                                                                   <  2:594493/150‑1 (MQ=255)
                                                   cAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCgag                                                                                                   >  2:839720/1‑150 (MQ=255)
                                                    aaaTGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGATCCTCATATACTGGCTCgaga                                                                                                  >  2:262068/1‑150 (MQ=255)
                                                    aaaTGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCgaga                                                                                                  >  1:458966/1‑150 (MQ=255)
                                                        gagaATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaac                                                                                              <  1:1300312/150‑1 (MQ=255)
                                                               atCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCaaa                                                                                       >  2:1297074/1‑150 (MQ=255)
                                                                tCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCg                                                                                                     >  1:575897/1‑135 (MQ=255)
                                                                    tttCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCAt                                                                                  <  2:769788/150‑1 (MQ=255)
                                                                     ttCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGAATATTGTCATGGGGCGGTTGATGAGCAAGCATGTTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATa                                                                                 <  1:237954/150‑1 (MQ=255)
                                                                          ccACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACAGGCTCGAGATTACAACCAAATGCATAAAAtt                                                                            >  1:473833/1‑150 (MQ=255)
                                                                           cacaGTTGCGCCAATTCAGCAGAACGAATGACGATGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTg                                                                           <  1:1143189/150‑1 (MQ=255)
                                                                           cacaGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTg                                                                           >  2:1352408/1‑150 (MQ=255)
                                                                            acaTTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCGCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGATATAACAACCAAATGCATAAAATTgg                                                                          <  2:1299390/150‑1 (MQ=255)
                                                                            acaGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTgg                                                                          <  1:1203784/150‑1 (MQ=255)
                                                                             aaGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTggg                                                                         <  2:1415472/149‑1 (MQ=255)
                                                                               gTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGaa                                                                       >  2:55417/1‑150 (MQ=255)
                                                                                 tGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGaaaa                                                                     >  1:1016257/1‑150 (MQ=255)
                                                                                     ccAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGc                                                                 <  1:500282/150‑1 (MQ=255)
                                                                                     ccAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGc                                                                 <  1:652932/150‑1 (MQ=255)
                                                                                      cAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGGTTGATCATTGCCATAATGAACCCTGAACAGCATAAACTGGCTCGAGAAAACAACCAAATGAATAAAATTGGGAAAATGGCa                                                                >  2:704387/1‑150 (MQ=255)
                                                                                        aTTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGa                                                              <  1:430316/150‑1 (MQ=255)
                                                                                           cagcagAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGc                                                           >  2:1583487/1‑150 (MQ=255)
                                                                                           cagcagAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCACATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGc                                                           >  2:123199/1‑150 (MQ=255)
                                                                                                  aCGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGACCCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGc                                                    >  1:133820/1‑150 (MQ=255)
                                                                                                  aCGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGc                                                    <  1:480758/150‑1 (MQ=255)
                                                                                                  aCGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGc                                                    >  2:430867/1‑150 (MQ=255)
                                                                                                   cGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGTCAGAAGCGTTTCGCa                                                   >  2:894376/1‑150 (MQ=255)
                                                                                                    gAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGATCCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATTGCAGAAGCCTTACGcct                                                  >  1:251915/1‑148 (MQ=255)
                                                                                                    gAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCAt                                                  >  2:1238286/1‑150 (MQ=255)
                                                                                                      aTGACGAAGCTCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAATCCTGACCCTCATAAACTGTCTCTATATAACAACCAACTGCATAAAATTGGGAATCTGGAAGAACCGTTTCGCATGc                                                >  2:316562/1‑150 (MQ=255)
                                                                                                           gAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAGCCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGTAAATGGCAGAAGCGTTTCGCATGCGtttt                                           >  2:677851/1‑150 (MQ=255)
                                                                                                            aaGCGCATTTGGTATATTGTCAGGTGGCGTTTGATGAGCAAGCAAGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAAAAACCAAATGCATAAAATTTGTAAAATGGCAGAATCGTTTCGCATGCGttttt                                          >  2:1630622/1‑150 (MQ=255)
                                                                                                            aaGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGatttt                                          >  2:165403/1‑150 (MQ=255)
                                                                                                             aGCGCTTTTGGTATATTGTCAGGGGGCGTTTGATGACCAAGCATGGTTGCTCATTGCCATTCTGAAAACTGACCCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTATTTg                                         <  2:840183/150‑1 (MQ=255)
                                                                                                             aGCGCATTTGGTATTTTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTg                                         <  2:332519/150‑1 (MQ=255)
                                                                                                             aGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGATTGGTTGCTCATTGCGATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGTGTTTCGCATGCGTTTTTg                                         <  1:382381/150‑1 (MQ=255)
                                                                                                                gcATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCGCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGGTTCGCATGCGGGTTTGAAt                                      <  2:114348/150‑1 (MQ=255)
                                                                                                                 cATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGAAtt                                     <  2:1649113/150‑1 (MQ=255)
                                                                                                                  ttttGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGAAttt                                    <  2:1728102/149‑1 (MQ=255)
                                                                                                                   tttGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGAATTta                                   >  1:1432917/1‑150 (MQ=255)
                                                                                                                     tGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGAATTtata                                 >  2:738866/1‑150 (MQ=255)
                                                                                                                     tGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAAACAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGAATTtata                                 >  1:712547/1‑150 (MQ=255)
                                                                                                                      ggTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGAATTTatat                                <  2:458966/150‑1 (MQ=255)
                                                                                                                      ggTATATTGTCAGGGGGCGGTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGAATTTatat                                <  2:923106/150‑1 (MQ=255)
                                                                                                                        tataTTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATACACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGAATTTAtatta                              <  2:1502165/150‑1 (MQ=255)
                                                                                                                        tataTTGTCAGGGGGCGTTTGATGAGCAACCATGGTTGCTCATTGCAATTCTGAAACCTGAACCTCATTAAGTGGCTCGAGATAACATCCAAATGCTTAATATTGGGAAAATGGCAGAAGCGTTTCGCATGCTTTTTTGTATTTttatta                              >  1:168294/1‑150 (MQ=255)
                                                                                                                          tatTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTAAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGAATTTATATTATg                            <  2:979360/150‑1 (MQ=255)
                                                                                                                             tGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGAATTTATATTATGAAt                         >  2:1540353/1‑150 (MQ=255)
                                                                                                                              gTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGAATTTATATTATGAATa                        >  2:1107960/1‑150 (MQ=255)
                                                                                                                                 aGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGAATTTATATTATGAATAACa                     >  2:1081508/1‑150 (MQ=255)
                                                                                                                                     ggCGTTTGATGAGCAAGCATGGTAGCTCATTGCAATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATTCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGAATTTATATTATGAATAACATACa                 <  2:276924/150‑1 (MQ=255)
                                                                                                                                      gCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGAATTTATATTATGAATAACATACaa                >  1:712419/1‑150 (MQ=255)
                                                                                                                                        gTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAAGCTGAACCTCATAAACTGGCTCGAGATAACAACCTAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGAATTTATATTATGAATAACATACAAAt              <  2:983892/150‑1 (MQ=255)
                                                                                                                                          ttGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGAATTTATATTATGAATAACATACAAATaa            <  1:865739/150‑1 (MQ=255)
                                                                                                                                          gcaatgaGCAACCATGATTGCTCATTGCCAGTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCTGAAGCGTTTCGCATGCGTTTTTGAATTTATATTATGAATAACATACaaaata            >  2:591669/4‑147 (MQ=255)
                                                                                                                                           tgatgaGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGAATTTATATTATGAATAACATACAAATAAg           >  2:10322/1‑150 (MQ=255)
                                                                                                                                           caatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGAATTTATATTATGAATAACATACAAATAAg           >  1:655671/3‑150 (MQ=255)
                                                                                                                                           caatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAAAAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGAATTTATATTATGAATAACATACAAATAAg           >  2:242088/3‑150 (MQ=255)
                                                                                                                                            gatgaGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGAATTTATATTATGAATAACATACAAATAAGa          <  2:387409/150‑1 (MQ=255)
                                                                                                                                            aatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGAATTTATATTATGAATAACATACAAATAAGa          >  1:1196485/2‑150 (MQ=255)
                                                                                                                                             atgaGCAAGCATGGTAGCTCATTGCCATTCTGAAACCTGAACCTCAAAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATTGAAGAAGCGTTTCGCCTGCGTTATTGAATTTATATTATGAATAACATACAAATAAGaa         >  1:283572/1‑150 (MQ=255)
                                                                                                                                              tgaGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGACCCTCATAAACTGGCTCGAGATAACACCCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTGACGCATGCGTTTTTGAATTTATATTATGAATAACATACAAATAAGaaa        >  2:616655/1‑150 (MQ=255)
                                                                                                                                               gaGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGAATTTATATTATGAATAACATACAAATAAGAAAc       <  2:193034/150‑1 (MQ=255)
                                                                                                                                               gaGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTGTTGAATTTATATTATGAATTACATACAAATAAGAAAc       >  2:1002542/1‑150 (MQ=255)
                                                                                                                                                 gcaagcaTGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGAATTTATATTATGAATAACATACAAATAAGAAACTa     >  1:807432/1‑150 (MQ=255)
                                                                                                                                                  caagcaTGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAAGTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGAATTTATATTATGAATAACATACAAATAAGAAACTAt    >  1:1079788/1‑150 (MQ=255)
                                                                                                                                                    agcaTGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGAATTTATATTATGAATAACATACAAATAAGAAACTATCa  <  2:545097/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCTTTACCTGGCCATTAATCAAATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGAATTTATATTATGAATAACATACAAATAAGAAACTATCA  >  NC_000913/252440‑252737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: