Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,247,032 T→C 7.5% intergenic (‑263/+31) cirA ← / ← lysP colicin IA outer membrane receptor and translocator; ferric iron‑catecholate transporter/lysine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,247,0320TC 7.5% 336.7 / 13.4 122intergenic (‑263/+31)cirA/lysPcolicin IA outer membrane receptor and translocator; ferric iron‑catecholate transporter/lysine transporter
Reads supporting (aligned to +/- strand):  ref base T (47/62);  new base C (6/3);  total (53/69)
Fisher's exact test for biased strand distribution p-value = 2.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.20e-01

Only 100 of 125 total aligned reads displayed.

ATCCCAGAAAGCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCC  >  NC_000913/2246883‑2247180
                                                                                                                                                      |                                                                                                                                                    
atcCCAGAAAGCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGt                                                                                                                                                      <  2:778515/150‑1 (MQ=255)
 tcCCAGAAAGCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGtt                                                                                                                                                     >  2:579069/1‑150 (MQ=255)
 tcCCAGAAAGCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGtt                                                                                                                                                     <  2:1347329/150‑1 (MQ=255)
  cccAGAAAGCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTg                                                                                                                                                    <  2:1187984/150‑1 (MQ=255)
  cccAGAAAGCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTg                                                                                                                                                    <  2:434464/150‑1 (MQ=255)
  cccAGAAAGCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTg                                                                                                                                                    <  1:1651018/150‑1 (MQ=255)
     aGAAAGCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTgaga                                                                                                                                                 <  1:579268/150‑1 (MQ=255)
         aGCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGc                                                                                                                                             <  1:553022/150‑1 (MQ=255)
          gCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCt                                                                                                                                            >  1:1531421/1‑150 (MQ=255)
          gCCGGAACAGCCTATGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCt                                                                                                                                            <  2:604549/150‑1 (MQ=255)
           ccGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCtt                                                                                                                                           >  2:105226/1‑150 (MQ=255)
              gAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGc                                                                                                                                        <  2:834151/150‑1 (MQ=255)
               aaCAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCtttttc                                                                                                                                       <  1:1699937/150‑5 (MQ=255)
               aaCAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCAACCGAGAGGGCtttttc                                                                                                                                       <  1:692712/150‑5 (MQ=255)
                aCAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCa                                                                                                                                       <  1:1672410/150‑1 (MQ=255)
                aCAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGATTAGCaa                                                                                                                                      >  2:452506/1‑150 (MQ=255)
                 cAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACTATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCaag                                                                                                                                     <  2:398196/150‑1 (MQ=255)
                 cAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCaag                                                                                                                                     <  2:144962/150‑1 (MQ=255)
                   gCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCaagga                                                                                                                                   >  2:800267/1‑150 (MQ=255)
                   gCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCaagga                                                                                                                                   >  2:621446/1‑150 (MQ=255)
                    ccTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCaaggaa                                                                                                                                  >  2:174065/1‑150 (MQ=255)
                       ctGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAggg                                                                                                                               >  2:774467/1‑150 (MQ=255)
                        tGATTTGTCAGCCTTTTTTGATTGCGGAGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGgga                                                                                                                              <  2:412385/150‑1 (MQ=255)
                         gATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGggag                                                                                                                             <  1:1018135/150‑1 (MQ=255)
                          aTTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGggagg                                                                                                                            <  1:621446/150‑1 (MQ=255)
                            ttGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGaa                                                                                                                          <  1:1621501/150‑1 (MQ=255)
                              gTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGaaaa                                                                                                                        >  2:1210940/1‑149 (MQ=255)
                                cAGCCTTTTTTGATAGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACtt                                                                                                                      >  2:1340150/1‑150 (MQ=255)
                                   cattttttGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTAtt                                                                                                                   <  1:326513/148‑1 (MQ=255)
                                    cTTTTTTGATTGCGGCGTAACGAGAATGCAACCGATACCAATTCTCAAGCCAAAGGTTAAATTTTATATCCGCATGATTTGTCAGGGAAAGGGAATTGAAAAAGCCCTCTAGTTTGAGAGGGCGTAGCAAGGAAGGGAGTACACTTAatt                                                                                                                  >  2:858573/1‑150 (MQ=255)
                                     ttttttGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTc                                                                                                                 <  2:1085802/150‑1 (MQ=255)
                                     ttttttGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGACAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTc                                                                                                                 >  1:1392565/1‑150 (MQ=255)
                                      tttttGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCt                                                                                                                <  2:451623/150‑1 (MQ=255)
                                        tttGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTa                                                                                                              <  1:30818/150‑1 (MQ=255)
                                          tGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATc                                                                                                            >  2:498425/1‑150 (MQ=255)
                                           gATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCg                                                                                                           <  1:1613900/150‑1 (MQ=255)
                                                     tAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGa                                                                                                 >  2:960468/1‑150 (MQ=255)
                                                     tAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATAGAAAAAGCCATCACGGTTCAGAGGGCTAAGCAAGGAATGGAGCAAAATTATTTCTTAACGTTCTGCGGGa                                                                                                 >  2:517399/1‑150 (MQ=255)
                                                     tAACGAAAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAAAGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGa                                                                                                 <  2:1100926/150‑1 (MQ=255)
                                                       aCGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAAc                                                                                               <  1:694040/150‑1 (MQ=255)
                                                        cGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACt                                                                                              <  1:611626/150‑1 (MQ=255)
                                                         gATAATGCAAACGATAACAATTATCAATCCAAATGTTAGATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACtt                                                                                             <  1:504814/150‑1 (MQ=255)
                                                         gATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGATGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACtt                                                                                             >  2:564664/1‑150 (MQ=255)
                                                          aTAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTc                                                                                            >  2:1520007/1‑150 (MQ=255)
                                                               gCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTc                                                                                       <  1:573550/150‑1 (MQ=255)
                                                                cAAGCGATAACAATTATCAATCCAAATGTTAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGc                                                                                     >  2:94103/1‑150 (MQ=255)
                                                                 aaaCGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGc                                                                                     >  1:925709/1‑150 (MQ=255)
                                                                 aaaCGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGc                                                                                     >  1:622141/1‑150 (MQ=255)
                                                                   aCGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTg                                                                                   >  1:579711/1‑150 (MQ=255)
                                                                    cGATAACTATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAATTGTAATTGAAAAAGCGCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGt                                                                                  <  2:255595/150‑1 (MQ=255)
                                                                      taaaCAATTATAAATACAAAAGGTAAAATTTACTTCCGCATTCTTTTGCACGGAAATGGAAATTAAAAAGCCCTCACGGTTGTGCTGGGCTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAg                                                                                <  1:733789/148‑1 (MQ=255)
                                                                        aaCAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCg                                                                              >  2:1034000/1‑150 (MQ=255)
                                                                         aCAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGATAGGGCTTAGCAAGGAAGGGAGGAAACTTATATCTTATCTTTCTGCGGGAACTTCATTTCGCTGTAGctt                                                                             >  2:1300676/1‑148 (MQ=255)
                                                                          cAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTa                                                                            >  2:905555/1‑150 (MQ=255)
                                                                           aaTTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTAc                                                                           <  2:1425199/150‑1 (MQ=255)
                                                                            aTTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACg                                                                          <  2:1484436/150‑1 (MQ=255)
                                                                              tATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGaa                                                                        >  1:1209403/1‑150 (MQ=255)
                                                                                   aTCCAAATTTTAAAATTTATATCCGGATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAg                                                                   <  1:41237/150‑1 (MQ=255)
                                                                                      cAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGGTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTc                                                                <  1:1554794/150‑1 (MQ=255)
                                                                                         aTGTTAAATTTTATATCCGCATGATTTGTCTGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCtt                                                             <  1:248667/150‑1 (MQ=255)
                                                                                         aTGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCtt                                                             <  2:1218553/150‑1 (MQ=255)
                                                                                         aTGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCtt                                                             <  2:1349230/150‑1 (MQ=255)
                                                                                          tGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCttt                                                            <  1:774467/150‑1 (MQ=255)
                                                                                           gTTAAATTTTATATCCGCATGATTTGACAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTa                                                           <  2:1392565/150‑1 (MQ=255)
                                                                                            ttAAATTTTATATCCTCATGATTTTTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTaa                                                          <  1:930097/150‑1 (MQ=255)
                                                                                            ttAAATTTTATATCCGCATTATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGGTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGTGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTaa                                                          <  1:1222329/150‑1 (MQ=255)
                                                                                                aTTTTATATCCGCATGATTTGACAGGGAAATTGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGTAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGAACGAAGTGAGTTCCTTTAATCac                                                      >  2:389226/1‑149 (MQ=255)
                                                                                                  tttATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCt                                                    <  1:1415858/150‑1 (MQ=255)
                                                                                                   ttATATCCTAATGATTTTTCAGTGAATTTTAATTGAAAAAGCACTATCGGGTTAGAGGGCTTAGCAAGGAAGGTAGGAAACTTATTTCTTATCGTTATGCTGGAACTTCATTTAGCTGTAGCGTACTAAGTGAGTTCCTTTAATCAGCtt                                                   <  1:1559511/150‑1 (MQ=255)
                                                                                                     atatCCGGAGGATTTGGCACGGAAATGGAATTGAAACAGCCCTCTGGGTTGAGAGGGCTTATCTATGAAGGGTGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGt                                                 <  1:1068496/150‑1 (MQ=255)
                                                                                                       atCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAg                                               >  1:798859/1‑150 (MQ=255)
                                                                                                        tCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGc                                              <  1:473079/150‑1 (MQ=255)
                                                                                                          cGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCg                                            >  1:800726/1‑150 (MQ=255)
                                                                                                             aTGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAAc                                         >  2:514281/1‑150 (MQ=255)
                                                                                                              tGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAAcc                                        >  2:40378/1‑150 (MQ=255)
                                                                                                                    gTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAaataa                                  >  1:1023723/1‑150 (MQ=255)
                                                                                                                    gTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAaataa                                  >  2:1707011/1‑150 (MQ=255)
                                                                                                                    gTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAaataa                                  >  1:620112/1‑150 (MQ=255)
                                                                                                                      cAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATATCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATc                                >  2:651780/1‑150 (MQ=255)
                                                                                                                         ggAAATGGATTTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAgg                             <  2:554033/150‑1 (MQ=255)
                                                                                                                         ggAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAgg                             >  1:1138925/1‑150 (MQ=255)
                                                                                                                          taaaTGTAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGCGAACATAATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGa                            <  1:93798/149‑1 (MQ=255)
                                                                                                                            aaTGGAATTTAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAAc                          <  1:222731/150‑1 (MQ=255)
                                                                                                                            aaTGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAAc                          <  2:525995/150‑1 (MQ=255)
                                                                                                                            aaTGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTGCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAAc                          <  1:1324685/150‑1 (MQ=255)
                                                                                                                               ggAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGc                       <  1:458997/150‑1 (MQ=255)
                                                                                                                                gAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCg                      >  2:51463/1‑150 (MQ=255)
                                                                                                                                 aaTTGAACAAGCCCTCTCGCGTGAGAGGGCGTAGCAAGGAAGGGAGGAAACTTATTTCATATCGTTCTGCGGGAACTTCATTTCGCTGGAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCgg                     <  2:255392/150‑1 (MQ=255)
                                                                                                                                  aTTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGATCCTTTAATCAGCTTGTAGCCGAACCAAATAATCATGAACTGCggg                    >  2:302031/1‑150 (MQ=255)
                                                                                                                                      tgctaaGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATa                 >  1:1342125/5‑150 (MQ=255)
                                                                                                                                       gctaaGCCCTCTCAACCGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATAc                >  1:914878/4‑150 (MQ=255)
                                                                                                                                       gctaaGCCCTCTCAACCGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGAAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATAc                >  2:1283582/4‑150 (MQ=255)
                                                                                                                                        ctaaGCCCTCTCAACCGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGCACAGCGGGATAcc               >  2:1587352/3‑150 (MQ=255)
                                                                                                                                        ctaaGCCCTCTCAACCGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATAcc               >  1:96527/3‑150 (MQ=255)
                                                                                                                                        aaaaGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATAcc               <  2:1300551/150‑1 (MQ=255)
                                                                                                                                           aGCCCTCTCAACCGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATACCAat            >  1:1249560/1‑150 (MQ=255)
                                                                                                                                                tctcGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACg       <  1:105226/150‑1 (MQ=255)
                                                                                                                                                 ctcGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGt      >  2:1175634/1‑150 (MQ=255)
                                                                                                                                                    ggTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGc   <  2:622141/150‑1 (MQ=255)
                                                                                                                                                     gTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGcc  >  1:87479/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
ATCCCAGAAAGCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCC  >  NC_000913/2246883‑2247180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: