Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,267,748 G→A 5.6% intergenic (+37/‑81) yeiQ → / → yeiR putative NAD‑dependent D‑mannonate oxidoreductase/Zn‑stimulated GTPase involved in zinc homeostasis; mutants are cadmium and EDTA sensitive; Zn(2+) binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,267,7480GA 5.6% 335.6 / 6.2 107intergenic (+37/‑81)yeiQ/yeiRputative NAD‑dependent D‑mannonate oxidoreductase/Zn‑stimulated GTPase involved in zinc homeostasis; mutants are cadmium and EDTA sensitive; Zn(2+) binding protein
Reads supporting (aligned to +/- strand):  ref base G (56/44);  new base A (4/2);  total (60/47)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01

Only 100 of 108 total aligned reads displayed.

GGTGATGATCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTA  >  NC_000913/2267601‑2267897
                                                                                                                                                   |                                                                                                                                                     
 gTGATGATCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAACGCGGATGAAGCTCACCTTCGTcc                                                                                                                                                    >  1:168405/1‑150 (MQ=255)
 gTGATGATCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTcc                                                                                                                                                    >  1:300368/1‑150 (MQ=255)
  tgatgaTCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCg                                                                                                                                                   >  2:646960/1‑150 (MQ=255)
  tgatgaTCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGACGAAGGTGAGCTTCATCCg                                                                                                                                                   <  1:1421420/150‑1 (MQ=255)
   gatgatCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACCCCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGc                                                                                                                                                  >  2:1190496/1‑150 (MQ=255)
   gatgatCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGc                                                                                                                                                  <  2:55150/150‑1 (MQ=255)
    atgatCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGACGAAGGTGAGCTTCATCCGct                                                                                                                                                 <  1:1567360/150‑1 (MQ=255)
     tgatCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGctc                                                                                                                                                >  1:800360/1‑150 (MQ=255)
      gatCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGctct                                                                                                                                               <  2:896403/150‑1 (MQ=255)
        tCTGCGAGATAACCCGCAGTTTGTGCAGGCCATCGACCAATCGTGGCAACAAAGCGTACAATGCGGCGCACATCAGGCTCTATTAAACCCCCTCATAATTTCACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTcc                                                                                                                                             <  2:1598552/150‑1 (MQ=255)
        tCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTcc                                                                                                                                             >  1:1449276/1‑150 (MQ=255)
         cTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTccc                                                                                                                                            >  2:54188/1‑150 (MQ=255)
          tGCCAGCTAACCCGCATTTTGTGCAGGCCATCGAACACGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTcccc                                                                                                                                           <  1:1431760/150‑1 (MQ=255)
          tGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATATAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTcccc                                                                                                                                           >  1:62386/1‑150 (MQ=255)
           gCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCGCCTTCGTCCGCTCTCCCCt                                                                                                                                          >  1:414995/1‑150 (MQ=255)
           gCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCt                                                                                                                                          >  1:1679201/1‑150 (MQ=255)
                  aaCCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCttt                                                                                                                                   >  2:1321857/1‑150 (MQ=255)
                  aaCCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCttt                                                                                                                                   <  1:844910/150‑1 (MQ=255)
                    cccGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTc                                                                                                                                 <  1:908109/150‑1 (MQ=255)
                    cccGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTc                                                                                                                                 >  1:390478/1‑150 (MQ=255)
                      cGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTg                                                                                                                               <  2:1686599/150‑1 (MQ=255)
                      cGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTg                                                                                                                               >  1:337526/1‑150 (MQ=255)
                      agCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATATCTGCGGTTAAAGCGGATGAAGCTAACCTTCGTCCACTCTCCCCTTCTATATTCTg                                                                                                                               >  1:337529/2‑150 (MQ=255)
                            ttGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCtttt                                                                                                                         <  1:1493634/150‑1 (MQ=255)
                              gtgCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACCCCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCtttttt                                                                                                                       >  2:1133068/1‑150 (MQ=255)
                               tgCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTa                                                                                                                      <  1:64664/150‑1 (MQ=255)
                               tgCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTCAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTa                                                                                                                      <  1:1696334/150‑1 (MQ=255)
                                     ccATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAgg                                                                                                                <  1:367576/150‑1 (MQ=255)
                                     ccATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAgg                                                                                                                <  2:62386/150‑1 (MQ=255)
                                      cATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCGTTTTTAGCCAGGa                                                                                                               >  1:1384063/1‑150 (MQ=255)
                                       aTCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGAt                                                                                                              >  1:1522698/1‑150 (MQ=255)
                                        tCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGAtt                                                                                                             <  2:28868/150‑1 (MQ=255)
                                          gAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGAGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCACCTTCTCTTTTCTGCCTTTTTTAGACAGGATTaa                                                                                                           >  1:1554914/1‑150 (MQ=255)
                                           aaCAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAAc                                                                                                          >  1:1473562/1‑150 (MQ=255)
                                           aaCAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAAc                                                                                                          <  2:644144/150‑1 (MQ=255)
                                            aCAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACg                                                                                                         >  1:104582/1‑150 (MQ=255)
                                               aGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTc                                                                                                      <  1:657590/150‑1 (MQ=255)
                                                 ccTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAg                                                                                                    <  2:803346/150‑1 (MQ=255)
                                                  cTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGt                                                                                                   >  1:878344/1‑150 (MQ=255)
                                                   tGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGtt                                                                                                  <  2:884327/150‑1 (MQ=255)
                                                      caacaaATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAAc                                                                                               <  1:550478/150‑1 (MQ=255)
                                                       aacaaATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACt                                                                                              >  1:362723/1‑150 (MQ=255)
                                                       aacaaATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACt                                                                                              >  2:705678/1‑150 (MQ=255)
                                                           aaTCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTAcc                                                                                          <  2:1201319/150‑1 (MQ=255)
                                                           aaTCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCGCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTAcc                                                                                          <  2:248382/150‑1 (MQ=255)
                                                               gTACAATTCGGCGCACATCAGGCGCTATTACACCCCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACATTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTAACAagag                                                                                      >  2:322875/1‑150 (MQ=255)
                                                               gTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCagag                                                                                      >  2:360158/1‑150 (MQ=255)
                                                                    aTTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTg                                                                                 <  2:878344/150‑1 (MQ=255)
                                                                     ttCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGc                                                                                <  2:390478/150‑1 (MQ=255)
                                                                      tCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCg                                                                               <  1:433460/150‑1 (MQ=255)
                                                                       cGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCgg                                                                              <  2:1324949/150‑1 (MQ=255)
                                                                         gcgcACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAg                                                                            >  1:1515496/1‑150 (MQ=255)
                                                                            cacaTCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCg                                                                         >  1:792457/1‑150 (MQ=255)
                                                                            cacaTCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGt                                                                        >  2:181860/1‑150 (MQ=255)
                                                                              caTCAGGCGCTATTAAACACACTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACATTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGtt                                                                       >  1:1040635/1‑150 (MQ=255)
                                                                                 cccgcgcTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGAGGAAGCTCACCTTCTTCCGCTCTCCCCTTCTCTTTTCTGCCTTTATTAGCCAGGATTAACGCTCAGTTAACTTACCACAGTATTGCGGAGGCGTtgtg                                                                    <  2:1533024/147‑1 (MQ=255)
                                                                                 cAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTtgtg                                                                    <  2:529522/150‑1 (MQ=255)
                                                                                 cAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTtgtg                                                                    >  2:705569/1‑150 (MQ=255)
                                                                                        tATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGa                                                             >  1:1454812/1‑150 (MQ=255)
                                                                                             aaCACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCGTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAAc                                                        >  2:562452/1‑150 (MQ=255)
                                                                                              acacCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAAcc                                                       >  2:1698372/1‑150 (MQ=255)
                                                                                                 cccTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCtca                                                    >  2:793302/1‑150 (MQ=255)
                                                                                                  ccTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCGTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTATCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCtcat                                                   >  1:438951/1‑150 (MQ=255)
                                                                                                  ccTCAAAATTAAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCTAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCtcat                                                   >  1:650185/1‑150 (MQ=255)
                                                                                                      aaaaTTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCAcc                                               >  1:1210130/1‑150 (MQ=255)
                                                                                                       aaaTTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCg                                              <  1:1582123/150‑1 (MQ=255)
                                                                                                        aaTTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCgg                                             <  2:300368/150‑1 (MQ=255)
                                                                                                           ttAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGttt                                          <  1:705678/150‑1 (MQ=255)
                                                                                                             aaCGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTtc                                        >  2:1241342/1‑150 (MQ=255)
                                                                                                              aCGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTtct                                       >  2:407278/1‑150 (MQ=255)
                                                                                                              aCGATTTATGCGGTAAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCACGATTAACGCTCAGTTAACTTACAAGAGTATTGCGGAGCCGTTGTGAACAGGACCAACCTCATCACCGGTTTtct                                       >  2:550089/1‑150 (MQ=255)
                                                                                                               cGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCCCCGGTTTtctc                                      >  2:142541/1‑150 (MQ=255)
                                                                                                               cGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTtctc                                      <  1:646960/150‑1 (MQ=255)
                                                                                                               cGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTATTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTtctc                                      <  2:832721/150‑1 (MQ=255)
                                                                                                                gATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCg                                     <  1:1321857/150‑1 (MQ=255)
                                                                                                                     cTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGGTTTCTCGGCAGc                                >  1:350738/1‑150 (MQ=255)
                                                                                                                       gCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCgg                              >  1:1517920/1‑150 (MQ=255)
                                                                                                                          gggagaGCGGACGAAGGTGAGCTTCATCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGaa                           >  2:1413405/6‑150 (MQ=255)
                                                                                                                          gggagaGCGGACGAAGGTGAGCTTCATCCGCTCTCCCCTTCTCTGTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGCCCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGaa                           >  2:25494/6‑150 (MQ=255)
                                                                                                                           ttAAAGCGGATGAAGCTCACCTTCGTCCGCGCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGaaa                          <  2:725313/150‑1 (MQ=255)
                                                                                                                             aaaGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCCCCGGTTTTCTCGGCAGCGGGAAAAc                        >  2:1679302/1‑150 (MQ=255)
                                                                                                                             aaaGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAAc                        >  2:782509/1‑150 (MQ=255)
                                                                                                                                gCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCAc                     <  2:104582/150‑1 (MQ=255)
                                                                                                                                gCGGATGAAGCTCACCTTCGGCCGCTCTCCCCTACTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCAc                     <  2:141243/150‑1 (MQ=255)
                                                                                                                                gCGGACGAAGGTGAGCTTCATCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCAc                     >  1:1247605/1‑150 (MQ=255)
                                                                                                                                gCGGACGAAGGTGAGCTTCATCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCAc                     >  1:1014613/1‑150 (MQ=255)
                                                                                                                                      taaGCTCACCTTCGTCCGCTCACCCCTTCTATTTTCTGCCTTTTTTAGCCAGGTTTACCGCTCAGTTAACTTACCAGATTATTGCGGAGCCGTTGTGACCAGGACCAACCACATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGAt               <  2:1449276/149‑1 (MQ=255)
                                                                                                                                      gAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGAt               <  1:498376/150‑1 (MQ=255)
                                                                                                                                        aGCTCACCTTCGTCCGCTCTCCCCTTCTCATTTCTGCCGTTTTTAGCCAGGATTACCGCTCAGTTTACTTACCAGCGTATTGCGGACACGTTGTGCCCCGGCCCACCCTCATCACCGGTTTTCTCGCCAGCGGGCACACCacgacgcgtc             >  2:301186/1‑146 (MQ=255)
                                                                                                                                         gCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGAttct            >  2:965314/1‑150 (MQ=255)
                                                                                                                                           tCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCGTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGAttcttc          <  2:329176/150‑1 (MQ=255)
                                                                                                                                           tCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGAttcttc          <  1:979786/150‑1 (MQ=255)
                                                                                                                                            cACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCa         >  2:336626/1‑150 (MQ=255)
                                                                                                                                             aCCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGCGCCGTTGTGCCCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCAt        >  2:72948/1‑150 (MQ=255)
                                                                                                                                              ccTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCa                      <  1:1349898/135‑1 (MQ=255)
                                                                                                                                              ccTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCa                      >  2:1349898/1‑135 (MQ=255)
                                                                                                                                              ccTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATc       >  2:1414988/1‑150 (MQ=255)
                                                                                                                                                ttCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTg     <  1:327706/150‑1 (MQ=255)
                                                                                                                                                ttCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATAAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTg     <  1:493114/150‑1 (MQ=255)
                                                                                                                                                   gTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTACCGCTCAGTTAACATACCAGAGTATTGCGGAGCCGTTGTGACCAGGCCCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCGTCATATGTTa  >  2:134937/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GGTGATGATCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTTA  >  NC_000913/2267601‑2267897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: